Antibiotic Susceptibility Profile of Aeromonas Species Isolated from Wastewater Treatment Plant by Isoken H. Igbinosa, Anthony I. Okoh

“…Antibiotic susceptibility was determined using the disc diffusion method, and polymerase chain reaction (PCR) assay was employed for the detection of antibiotics resistance genes. …”
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Prevalence of Blood-Borne Viruses in Health Care Workers of a Northern District in Pakistan: Risk Factors and Preventive Behaviors by Muhammad Zuhaib Khan, Shahab Saqib, Sayed Irtiza Hussain Shah Gardyzi, Javaria Qazi

“…Prevalence was investigated using serological assays followed by real time polymerase chain reaction (RT-PCR) based characterization. …”
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Herpes Simplex Virus Pneumonia: Importance of Aspiration Etiology by Kentaro Odani, Mitsuhiro Tachibana, Rintaro Tamashima, Yutaka Tsutsumi

“…HSV-1 infection was confirmed by immunostaining with monospecific monoclonal antibodies and by type-specific real-time polymerase chain reaction. It is very likely that HSV pneumonia was provoked by aspiration of infected exudates from the upper airway (namely, sequential infection from the tongue, epiglottis, and trachea to lung). …”
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Absence of the Genetic Marker IS6110 from a Strain of Mycobacterium tuberculosis Isolated in Ontario by Susan T Howard, Matthew T Oughton, Albert Haddad, Wendy M Johnson

“…However, the insertion element IS6110, which is used for epidemiological tracing of M tuberculosis, was not detected in this strain by either restriction fragment length polymorphism analysis or by polymerase chain reaction. Two other genetic markers associated with the M tuberculosis complex, IS1081 and the direct repeat element, were present. …”
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Reference Gene and Protein Expression Levels in Two Different NAFLD Mouse Models by Layanne C. C. Araujo, Silvana Bordin, Carla R. O. Carvalho

“…Histological staining techniques were used to verify the morphology and quantify the amount of lipid droplets present in the liver. Real-time polymerase chain reaction and immunoblotting were employed for monitoring protein expression and gene expression levels, respectively. …”
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Impacts of Horticultural Mineral Oils and Two Insecticide Practices on Population Fluctuation of Diaphorina citri and Spread of Huanglongbing in a Citrus Orchard in Sarawak by Stephen Chan Teck Leong, Fatimah Abang, Andrew Beattie, Roland Jui Heng Kueh, Sing King Wong

“…Levels of HLB as determined by Polymerase Chain Reaction (PCR) were 42.2%, 9.4%, 11.4%, and 22.7%, respectively. …”
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Anti-amoebic effects of selected herbal extracts against Acanthamoeba species isolated from different borehole water samples from Budiriro District in Harare, Zimbabwe by Kudzai Fambisai, Petros Muchesa, Farisai Chidzwondo, Rumbidzai Mangoyi

“…Acanthamoeba castellannii species were isolated and confirmed present in Budiriro borehole water samples using non-nutrient agar, microscope, real-time Polymerase Chain Reaction (PCR), and gel electrophoresis. The selected plant samples, Murumanyama (Xeroderris stuhlmannii) bark extract, Munhundurwa (Solanum incanum) fruit extract and Mufandichimuka (Myrothamnus flabellifolius) stem and leaf extracts were then investigated for their anti-amoebic effects against the isolated Acanthamoeba castellannii, using agar well diffusion method. …”
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Candida Albicans Candidalysin ECE1 Gene − A Potent Virulence Factor for Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders by Mani B. Subramani, Krishnan Mahalakshmi, Bharathy Jaya, Sankar Leena Sankari, Venkatesan N. Kumar

“…Methods and Material: A total of 60 C. albicans isolates from OSCC (n = 23), OPMDs (n = 23) and healthy subjects (n = 14) were subjected to a polymerase chain reaction (PCR) for the detection of ECE1 gene. …”
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Circulating LncRNA Is Potentially Up-regulated in Coronary Artery Disease by Niloofar Avazpour, Mohammadreza Hajjari, Saeed Yazdankhah, Azita Sahni, Ali Mohammad Foroughmand

“…The expression level was examined by semi-quantitative reverse transcriptase polymerase chain reaction technique. Our data shows that expression of HOTAIR is up-regulated in blood samples of patients with CAD.…”
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Cardiopulmonary Arrest and Pulmonary Hypertension in an Infant with Pertussis Case Report by Patrick Ovie Fueta, Harry Onoriode Eyituoyo, Oghogho Igbinoba, Jon Roberts

“…Bordetella pertussis infection was confirmed on respiratory polymerase chain reaction. She was treated with antibiotics, ventilatory management, and other supportive care. …”
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Association of Low Expression of NUMB in Peripheral Blood with Acute Myocardial Infarction by Heyu Meng, Lihong Li, Jianjun Ruan, Yanqiu Chen, Zhaohan Yan, Jinsha Liu, Xiangdong Li, Cuiying Mao, Ping Yang

“…The real-time quantitative polymerase chain reaction was used to measure the mRNA expression level of the NUMB gene in peripheral blood. …”
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Investigation into the sero-molecular prevalence of Brucella melitensis in small ruminants in districts Mohmand and Charsadda Khyber Pakhtunkhwa Pakistan. by Sohaib Ul Hassan, Farhan Anwar Khan, Muhammad Shuaib, Muhammad Shahid, Said Sajjad Ali Shah, Shahrood Ahmed Siddiqui, Anthony Pokoo-Aikins, Ayman A Swelum

“…A total of 400 blood samples were collected from sheep and goats (n = 200 from each species) and analyzed by Rose Bengal precipitation test (RBPT), the indirect enzyme-linked immunosorbent assay (i-ELISA) and polymerase chain reaction (PCR). The findings of the study indicated 13.5% and 7% of sheep while 12.5% and 12.5% of goat's samples by RBPT and (i-ELISA) respectively. …”
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Evidence of High-Risk Human Papillomavirus in Esophageal Cancer in East Azerbaijan Province, Northwest of Iran by Zahra Sadeghian, Hossein Bannazadeh Baghi, Vahdat Poortahmasebi, Javid Sadeghi, Alka Hasani, Arezoo Azadi, Mahin Ahangar Oskouee

“…Methods. 140 paraffin-embedded specimens of esophageal tissues were investigated using nested-polymerase chain reaction (nested-PCR) with primer designing for the L1 region of HPV genome. …”
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ASPM Promotes the Progression of Anaplastic Thyroid Carcinomas by Regulating the Wnt/β-Catenin Signaling Pathway by Liang Jiang, Shuai Zhang, Ning An, Guoqing Chai, Changhong Ye

“…The data from two data sets (GSE76039 and GSE33630) were extracted and analyzed for the expression of ASPM, followed by a further validation in collected ATC patients using quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting. The effect of ASPM on cell proliferation, migration, invasion, and cell cycle was explored in ATC cell lines by in vitro inhibition of ASPM, while ASPM-mediated tumorigenicity was investigated in a xenograft tumor model. …”
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Comparison of different methods for identification of meat species in meat products by Yusuf Özşensoy, Seyda Şahin

“…The identification of meat species in meat products was investigated using Agar Gel Immunodiffusion (AGID), and Enzyme-Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction (PCR) methods.<p> <b>Results:</b> All of AGID, ELISA and PCR methods detected positivity for cattle meat in same samples. …”
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Determine the prevalence and fluoroquinolone resistance pattern of Escherichia coli ST131 among clinical isolates from vaginitis patients by Noor Ismeal Nasser, Mohammed Sabri Abdul-Razzaq, Nadia M. Al-Hili

“…Materials and Methods: A total of 150 high vaginal swabs were taken from female patients with aerobic vaginitis and were screened for the isolation and identification of E. coli ST131 using polymerase chain reaction technology that targets ST131-specific sequence polymorphisms in both the mdh and gyrB genes. …”
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COVID-19 and Pulmonary Tuberculosis Coinfection in a Moroccan Patient with Pulmonary Embolism: A Case Report and Literature Review by Imane Zouaki, Zakaria Chahbi, Mohamed Raiteb, Mohamed Zyani

“…We present a case of a sixty-seven-year-old male patient who was admitted to our COVID-19 emergency department with a diagnosis of COVID-19 pneumonia, confirmed by nasopharyngeal swab’s polymerase chain reaction (PCR) for detection of SARS-CoV-2. …”
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M2-like macrophage-derived exosomes inhibit osteoclastogenesis via releasing miR-1227-5p by Shan Chen, Jian Liu, Lilei Zhu

“…Osteoclastogenesis was assessed using tartrate-resistant acid phosphatase staining and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The molecular mechanism was evaluated using microarray analysis, RT-qPCR, dual-luciferase reporter analysis, and RNA pull-down assay. …”
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Detection of Helicobacter pylori in City Water, Dental Units' Water, and Bottled Mineral Water in Isfahan, Iran by Ahmad Reza Bahrami, Ebrahim Rahimi, Hajieh Ghasemian Safaei

“…In the present study, totally 200 water samples were collected in Isfahan province and tested for H. pylori by cultural method and polymerase chain reaction (PCR) by the detection of the ureC (glmM) gene. …”
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CircRNA FAT1 Regulates Osteoblastic Differentiation of Periodontal Ligament Stem Cells via miR-4781-3p/SMAD5 Pathway by Yu Ye, Yue Ke, Liu Liu, Tong Xiao, Jinhua Yu

“…The abilities of circFAT1 and miR-4781-3p in regulating PDLSC differentiation were analyzed by western blot, reverse transcription-polymerase chain reaction (RT-PCR), alkaline phosphatase (ALP), and Alizarin red staining (ARS). …”
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