A new atypical splice mutation in PKD2 leading to autosomal dominant polycystic kidney disease in a Chinese family by Junlin Zhang, Yiting Wang, Yingwang Zhao, Fang Liu

“…In addition, reverse transcription-polymerase chain reaction (RT-PCR) was performed to test for abnormal splicing and assess its potential pathogenicity. …”
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A Prospective Study of Clinical Features of Anterior Uveitis in Taiwan by Wei-Yu Chiang, Shih-Chou Chen, Shwu-Jiuan Sheu, Hsi-Kung Kuo

“…The clinical diagnoses, ocular presentations, and laboratory data, including the results of the aqueous polymerase chain reaction tests, were investigated in these patients. …”
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Investigation of west nile virus infection in brown bears (ursus arctos) in turkey by Yakup Yıldırım, Fethiye Çöven, Erdoğan Uzlu, Volkan Yılmaz, Emrah Çoban, Ayşegül Çoban, Çağan Hakkı Şekercioğlu

“…Reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of West Nile Virus nucleic acid.…”
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Signature Prediction Modeling in Cytolethal Distending Toxin-Producing Strains by Maryam Javadi, Mana Oloomi, Saeid Bouzari

“…It was shown that they could be used as biomarkers for research purposes and clinical diagnosis by polymerase chain reaction, or in vaccine development. cdt genes and several other genetic biomarkers were identified as signature sequences in CDT producer strains. …”
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Acute Pancreatitis as a Possible Unusual Manifestation of COVID-19 in Children by Niloufar Bineshfar, Alireza Mirahmadi, Fereshteh Karbasian, Elham Pourbakhtyaran, Abdollah Karimi, Mehdi Sarafi

“…He was diagnosed with acute pancreatitis based on an abnormal amylase level and abdomen computed tomography (CT) and later found to be infected by SARS-CoV-2, by a positive reverse transcriptase-polymerase chain reaction (RT-PCR) test.…”
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Immunological and Molecular Method for the Diagnosis of Human Bocavirus in Patients with Respiratory Infections in Mosul, Iraq by Anmar A. AlTaie, Noor Raad Abdulghany, Muhammad Abdul-Ghani Muhammad, Mohammad M. Salih, Iman Mutasher Aufi

“…The samples were stored in a viral transport medium until indirect enzyme-linked immunosorbent assay and real-time polymerase chain reaction (RT-PCR) were performed. Results: Out of 70 samples, the prevalence rate of immunoglobulin (Ig)M antibodies was 37 (53%), IgG was 33 (47%), and viral DNA for HBoV was 24 (34%), respectively. …”
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Maternal, fetal, and perinatal outcomes of COVID-19-infected pregnancies in Saudi Arabia: a meta-analysis and systematic review by Ahmad Alwazzan

“…Pregnancy cases only from Saudi Arabia and reverse transcription polymerase chain reaction confirmed COVID-19 positive were included. …”
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Visceral Leishmaniasis or Systemic Lupus Erythematosus Flare? by Sunny Garg, Mousumi Kundu, Amit Nandan Dhar Dwivedi, Lalit Prashant Meena, Neeraj Varyani, Asif Iqbal, Kamlakar Tripathi

“…Despite negative results of rk-39 test and bone marrow biopsy, a very high suspicion for visceral leishmaniasis (VL) led us to go for direct agglutination test (DAT) and polymerase chain reaction (PCR) for leishmanial antigen that revealed positive results. …”
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Systematic Screening for SARS-CoV-2 to Detect Asymptomatic Infections: An Epitome of Taiwan’s Outbreak by Chih-Chien Cheng, Chia-Chen Liu, Ju-Chuan Yen, Ting-Fang Chiu, Yi-Ning Liu, Thomas Y. Hsueh, Sheng-Huang Hsiao

“…As care providers and gatekeepers of infectious diseases, Taipei City Hospital has to perform regular polymerase chain reaction (PCR) results of admitted patients and healthcare workers (HCWs) to achieve these goals. …”
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Flavonoids-rich extract of Aframomum melegueta (black pepper) improves antioxidant status and modulates aging process in lead-induced neurotoxic Drosophila melanogaster by Mohammed Sani Jaafaru

“…The expression and activities of the phase II antioxidant and age-related enzymes were determined using quantitative polymerase chain reaction (qPCR) to assess the oxidative and longevity effects of the extract. …”
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Clinical Significance of Molecular Diagnostic Tools for Bacterial Bloodstream Infections: A Systematic Review by Jean Pierre Rutanga, Therese Nyirahabimana

“…The majority of these tools focus on amplification based techniques such as polymerase chain reaction (PCR) which allows the detection of nucleic acids (both DNA and small RNAs) that are specific to bacterial species and sequencing or nucleic acid hybridization that allows the detection of bacteria in order to reduce delay of appropriate antibiotic therapy. …”
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Genetic Analysis of CYP2C9 with Reference to Drug Response in Epilepsy Patients of Pakistan by Hafsa Maqbool, Tayyaba Saleem, Nadeem Sheikh, Aqsa Ashfaq

“…Genomic DNA was isolated from blood, and amplification of rs1799853 (430C > T) and rs1057910 was carried out by polymerase chain reaction. Genotypes of CYP2C9 SNPs were determined by Sanger’s sequencing. …”
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Genetic Study In a Pakistani Family Reveals Autosomal Recessive Type of Artemis Deficiency by Saddaf Ayub, Abeerah Zainub, Nida Shafi, Zara Khalid Khan, Lubna Siddique, Syed Irfan Raza

“…Genetic analysis was performed on patients and available parents. Polymerase Chain Reaction (PCR) based amplification and electrophoresis in patients, parents and control was performed. …”
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Rare but Not so Rare? The Evolving Spectrum of Whipple's Disease by John M Conly, B Lynn Johnston

“…Once considered the ideal case report, recent characterization of Tropheryma whippelii by means of broad range bacterial ribosomal DNA polymerase chain reaction (PCR) analysis (2,3) and its subsequent cultivation (4) has led to a veritable explosion of individual case reports, case series and hitherto unrecognized manifestations of the disease, such that it is now considered an underdiagnosed infectious disease (5). …”
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HEMAGGLUTINATION AND ANTIGENIC PROPERTIES OF AVIBACTERIUM PARAGALLINARUM ISOLATES RECOVERED IN THE RUSSIAN FEDERATION AND REPUBLIC OF BELARUS by A. V. Potehin, V. A. Yevgrafova, D. B. Andreychuk

“…No serological relatedness was identifed between the tested isolates and reference strain of serogroup A both using agglutination test (≤23.6%) and HI (≤12.2%). Polymerase chain reaction demonstrated that all the isolates recovered in the Russian Federation and Republic of Belarus in 2014-2016 belonged to serogroup B.…”
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Changes in Anterior Chamber Parameters Measured by Pentacam in Coronavirus Disease 2019 Infection: A Longitudinal Study by Saeed Shokoohi-Rad, Mojtaba Abrishami, Elham Bakhtiari, Asma Payandeh, Hamid-Reza Heidarzadeh

“…All subjects exhibited a confirmed diagnosis of COVID-19 with a positive result of nasopharyngeal reverse transcription-polymerase chain reaction evaluation. Corneal Pentacam (Oculus, Dutenhofen, Germany) imaging was performed at least two weeks after recovery from systemic COVID-19. …”
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Noninvasive Monitoring of mRFP1- and mCherry-Labeled Oncolytic Adenoviruses in an Orthotopic Breast Cancer Model by Spectral Imaging by Anton V. Borovjagin, Lacey R. McNally, Minghui Wang, David T. Curiel, Mary J. MacDougall, Kurt R. Zinn

“…Following intratumoral injection, localization and replication of each virus in orthotopic breast cancer xenografts were analyzed by spectral imaging and verified by quantitative polymerase chain reaction. Fluorescence in tumors increased up to 2,000-fold by day 4 and persisted for 5 to 7 weeks, showing oscillatory dynamics reflective of CRAd replication cycles. …”
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Brainstem and Cerebellar Involvement in Ramsay Hunt Syndrome by Vijay Letchuman, Charles D. Donohoe

“…Clinical awareness that Ramsay Hunt syndrome may also involve the brainstem and cerebellum is critical in evaluating the clinical neurologic findings and expanding the diagnostic workup to include brain magnetic resonance imaging and cerebrospinal fluid analysis, including varicella zoster polymerase chain reaction. Encephalitis requires longer duration administration of high-dose intravenous acyclovir in conjunction with steroids. …”
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Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses. by Kouichi Kitamura, Minetaro Arita

“…Reverse transcription-polymerase chain reaction (RT-PCR) assays targeting their VP1, VP4, and partial VP2 (VP4-pVP2) genomic regions are used for detection and identification. …”
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Detection of Leishmania donovani DNA within Field-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) in Three Cutaneous Leishmaniasis Endemic Foci of Kurunegala District, Sri La... by Tharaka Wijerathna, Nayana Gunathilaka, Kithsiri Gunawardena, Yoshito Fujii, Deepa Gunasekara

“…Field-caught sand flies were identified to the species level followed by DNA extraction. The polymerase chain reaction (PCR) was performed using species-specific primers to detect Leishmania donovani parasites. …”
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