Comparison of Illumina and Oxford Nanopore sequencing data quality for Clostridioides difficile genome analysis and their application for epidemiological surveillance by Semeh Bejaoui, Sofie Holtsmark Nielsen, Astrid Rasmussen, John Eugenio Coia, Dorte Terp Andersen, Tobias Bruun Pedersen, Martin Vad Møller, Marc Trunjer Kusk Nielsen, Dorte Frees, Søren Persson

“…Nanopore sequences exhibited an average of 640 base errors per genome (~ 0.015% substitution rate), which was reflected by the incorrect assignment of over 180 alleles in core genome multilocus sequence typing (cgMLST) analysis. …”
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New evidence on the Late Bronze – Early Iron Age human nutrition in the Crimea (northern Pontic Region) based on the stable isotope analysis and radiocarbon dating by Kulkova Marianna A., Kashuba Maya N., Kulkov Aleksandr M., Kozhukhovskaya Yuliya V., Tikhomirov Vitaliy A., Streltsov Mihail A.

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Iron deficiency, supplementation, and sports performance in female athletes: A systematic review by Michael Pengelly, Kate Pumpa, David Bruce Pyne, Naroa Etxebarria

“…Conclusion: High-level ID female athletes experience a negative impact on endurance performance, which can be improved by supplementing with ∼100 mg of elemental iron per day or bi-daily. The decrements in other performance parameters characterizing a range of sports coincide with the severity of iron deficiency.…”
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Global burden of burns among children and adolescents: a trend analysis from the global burden of disease study 2019 by Hao Zhu, Hao Zhu, Ke Wang, Xiong Liu, Jianfeng Ji, Peng Yang, Feng Xu

“…Sex, age, region, and SDI were used as stratification variables in the study to evaluate the prevalence of burns injuries among kids and teenagers across 204 nations and territories between 1990 and 2019. YLD rates per 100,000 people and incidence rates together with their respective average annual percentage changes (AAPC) were computed to evaluate burden trends. …”
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Point-of-care tests, diagnostic uncertainty and antimicrobial stewardship in the ICU: procalcitonin or PCR to aid antibiotic-stop decisions – an observational cohort study by Luke Moore, Suveer Singh, Martine Nurek, Nabeela Mughal, Timothy Lau, Archit Singhal

“…After a negative PCR-POCT and borderline-positive PCT result, a final antibiotic decision was taken.Measures Proportion of stop decisions before versus after test results per scenario. The association of the final decision with the clinician’s change in confidence, willingness to request the biomarker(s) and the case trajectory was determined.Results Fewer clinicians than expected stopped antibiotics versus baseline (36%, 94/264 vs 42%, 110/264, p=0.045). …”
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Prevalence and factors associated with burnout among healthcare providers at Kasulu district in Kigoma region, 2024: an analytical cross-sectional study in a primary healthcare set... by Omary Ubuguyu, Godbless Henry Mfuru, Khadija I Yahya-Malima

“…Significant factors included age 21–30 years (adjusted prevalence ratio (aPR) 1.55, 95% CI 1.06 to 2.27), nursing profession (aPR 2.19, 95% CI 1.07 to 4.48), off-site residence (aPR 1.23, 95% CI 1.02 to 1.49), 6 months to 2 years of experience (aPR 1.44, 95% CI 1.12 to 1.86), dissatisfaction with salary (aPR 1.77, 95% CI 1.02 to 3.07), poor colleague relationships (aPR 1.25, 95% CI 1.03 to 1.51), ≥4 night shifts/week (aPR 2.54, 95% CI 1.33 to 4.86), attending ≥41 patients per day (aPR 1.52, 95% CI 1.06 to 2.19) and lack of academic growth opportunities (aPR 1.62, 95% CI 1.15 to 2.29).Conclusion Rural settings like Kasulu face unique challenges, including limited resources and heavy workloads, exacerbating burnout among healthcare providers. …”
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Effect of packaging materials and storage temperature on the shelf stability of Awaze paste by Biadge Kefale, Biadge Kefale, Mulugeta Admasu Delele, Mulugeta Admasu Delele, Solomon Workneh Fanta, Solomon Abate

“…The samples stored in glass bottles at refrigeration temperature (4°C) were found to have a color (a*) value difference of 11.5 to 13.85, a yeast and mold count value of 3.2 log cfu/g, and a TBC value of 2.97 log cfu/g, which were acceptable after 300 days of storage as per the international food standards.ConclusionTaking into account all parameters including physical changes (color, mold growth, texture), acidity, yeast and mold, TBC, and LAB count, Awaze paste could be stored in glass bottles at 4°C for up to 300 days.…”
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Evaluating a targeted selective speech, language, and communication intervention at scale – Protocol for the Happy Talk cluster randomised controlled trial. [version 2; peer review... by Pauline Frizelle, Darren Dahly, Aoife O'Shea, Aileen Murphy, Cristina McKean

“…Seventy-two clusters will receive the intervention (12 participants per cluster). Parents and pre/school staff engage in group training and coaching in the form of 12 1-hour sessions for parents and four staff workshops, over the course of the pre/school year. …”
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准分子激光原位角膜磨镶术前后角膜非球面分析 by 黄国富, 王铮, 杨斌, 张晓晓, 邱平, 周胜, 陈家祺

“…高阶像差改变与术后无眩光各空间频率对比敏感度6.3,4.0,2.5,1.6,1.0,0.7（cycles per degree,cpd）成负相关（P〈0.05）；高阶像差改变 与低、中、高度眩光对比敏感度中高频空间频率6．3，4．0，2．5，1．6cpd成负相关（P〈0.05），与低频空间频率（1．0，0.cpd）无相关关系（P〉0.05）。…”
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Reimbursement policies of Swiss health insurances for the surgical treatment of symptomatic breast hypertrophy: a retrospective cohort study by Astrid Navarra, Daniel Schmauss, Reto Wettstein, Yves Harder

“…Primary outcome measures included median duration between the first request for reimbursement sent to the health insurance and the receipt of its approval, the number of requests needed per patient, as well as the number and type of additional outpatient visits conducted by specialists other than plastic surgeons, including the need for further diagnostic investigations and therapeutic measures. …”
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Ethnic inequalities in mental and physical multimorbidity in women of reproductive age: a data linkage cohort study by Amelia Jewell, Matthew Broadbent, Stephani Hatch, Mark Ashworth, Megan Pritchard, Sarah Dorrington, Louise Howard, Raquel Catalao

“…Objectives Explore inequalities in risk factors, mental and physical health morbidity in non-pregnant women of reproductive age in contact with mental health services and how these vary per ethnicity.Design Retrospective cohort study.Setting Data from Lambeth DataNet, anonymised primary care records of this ethnically diverse London borough, linked to anonymised electronic mental health records (‘CRIS secondary care database’).Participants Women aged 15–40 years with an episode of secondary mental health care between January 2008 and December 2018 and no antenatal or postnatal Read codes (n=3817) and a 4:1 age-matched comparison cohort (n=14 532).Main outcome measures Preconception risk factors including low/high body mass index, smoking, alcohol, substance misuse, micronutrient deficiencies and physical diagnoses.Results Women in contact with mental health services (whether with or without severe mental illness (SMI)) had a higher prevalence of all risk factors and physical health diagnoses studied. …”
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Combination of autoimmune hepatitis to non-alcoholic steatohepatitis (Clinical case) by A. A. Driga, M. V. Mayevskaya, V. T. Ivashkin

“…Treatment included budenofalk 9 mg per day, that was followed by normalization of transaminases within 3 months. …”
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Effects of exercise training on the nigrostriatal glutamatergic pathway and receptor interactions in Parkinson’s disease: a systematic review by Shahid Ishaq, Iqbal Ali Shah, Shin-Da Lee, Shin-Da Lee, Bor-Tsang Wu

“…The majority of studies (89%) employed treadmill training, while 11% used voluntary wheel running, with training protocols consisting of 5 days per week for 4 weeks.ResultsExercise training reduced extracellular glutamate (Glu) and increased the expression of GLT-1, GS, Gln, and mGluR2/3 while down-regulating VGULT1 in the presynaptic terminal of the glutamatergic neurons within the nigrostriatal pathway in PD animal models. …”
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Global, regional, and national disease burden attributable to high systolic blood pressure in youth and young adults: 2021 Global Burden of Disease Study analysis by Chuan He, Saien Lu, Haijie Yu, Yingxian Sun, Xueyao Zhang

“…In 2021, the mortality and DALY rates were 4.29 (95% UI, 3.29–5.28) and 263.37 (95% UI, 201.40–324.46) per 100,000 population, respectively. The overall HSBP-related burden was higher in males than in females, with increasing and decreasing trends for males and females, respectively. …”
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Factors affecting cadmium toxicity to rice germinated in soils collected from downstream areas of abandoned zinc mines by P. Chanpiwat, A. Numprasanthai

“…A total of 36 paddy fields that were reported to produce rice grain with cadmium contents exceeding the national standard for cadmium in rice (0.4 milligrams per kilogram) were randomly selected for composite soil sample collection. …”
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Overweight and obesity among adolescents: health-conscious behaviours, acceptance, and the health behaviours of their parents by Anna Lewandowska, Grzegorz Rudzki, Tomasz Lewandowski, Anna Bartosiewicz, Michał Próchnicki, Aleksandra Stryjkowska-Góra, Barbara Laskowska, Małgorzata Sierpińska, Sławomir Rudzki, Sergii Pavlov

“…Methods A cross-sectional study was conducted per the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines. …”
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Mixed Comparative Evaluation of a Training Program Dedicated to Cystic Fibrosis Reference Centers: Protocol for the Pilot Implementation of Shared Decision-Making in the Treatment... by Nora Moumjid, Constance Gotte, Sophie Hommey, Stéphanie Poupon Bourdy, Julie Haesebaert, Isabelle Durieu, Quitterie Reynaud

“…ResultsForty patients will be included (8 patients in each center), that is, 60 consultation observations (2 consultations per patient in the intervention groups given the modalities of the SDM process) will be conducted in 2025. …”
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The impact of precipitation, temperature, and soil moisture on wheat yield gap quantification: evidence from Morocco by Lahcen Ousayd, Terence Epule Epule, Salwa Belaqziz, Victor Ongoma, Abdelhakim Amazirh, Abdelghani Chehbouni

“…Additionally, losses due to wheat yield gaps were considerable, ranging between $ 194 and 891 per hectare. The revenue loss due to Yield Gap I ranged from 49 to 71%, while the loss due to Yield Gap II ranged from 240 to 444%, depending on the method used to calculate the wheat yield gap. …”
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Participant Compliance With Ecological Momentary Assessment in Movement Behavior Research Among Adolescents and Emerging Adults: Systematic Review by Shirlene Wang, Chih-Hsiang Yang, Denver Brown, Alan Cheng, Matthew Y W Kwan

“…For example, studies that only prompted participants once per day had higher compliance (91% vs 77%), but more frequent prompts provided more comprehensive data for researchers at the expense of increased participant burden. …”
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Effects of Agrochemicals on Soil Bio-Physico-Chemical Properties in Potato Production Systems in the Highlands of South Western Uganda. by Twebaze, Jeniffer

“…Soil macrofauna samples were collected three times per season, and the observed organisms were counted and recorded to determine their abundance, evenness, and richness. …”
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