Optimized for routine: highly sensitive fluorescent Telomeric Repeat Amplification Protocol (f-TRAP) by Silke Fähnrich, Anne Wedemann, Laura Steenpass, Wilhelm Gerhard Dirks

“…Here we present an optimized protocol for measuring TA that combines a fluorescently labeled bait primer and polymerase chain reaction (PCR) amplification with analytical capillary electrophoresis (CE) to achieve a detection limit of one telomerase-positive cell per ten thousand negative cells. In research laboratories today, a broad panel of TRAP assay protocols enables the assessment of the immortality of newly generated cell lines or the unambiguous evaluation of the reprogramming of induced pluripotent stem cells (iPSCs). …”
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Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis by Hong Pan, Ping Keun Lam, See W. Tong, Kevin K. Leung, Anthony Y. Teoh, Enders K. Ng

“…Sixteen pigs were divided into a 7 d group and 14 d group, each of which was further subdivided into an MSC-treated group and a control group. Five anastomoses per animal were established using LigaSure ForceTriad (Covidien, MA, USA), a tissue sealing system. …”
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Transcriptome size matters for single-cell RNA-seq normalization and bulk deconvolution by Songjian Lu, Jiyuan Yang, Lei Yan, Jingjing Liu, Judy Jiaru Wang, Rhea Jain, Jiyang Yu

“…ReDeconv introduces a scRNA-seq normalization approach, Count based on Linearized Transcriptome Size (CLTS), which corrects differential expressed genes typically misidentified by standard count per 10 K normalization, as confirmed by orthogonal validations. …”
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The History of a Hospital and Challenge of Expansion: CostEffectiveness and Surgical Coverage by Abdulrahman Hama Amin Hussein, Sanoor kadir Mahmood, Salah Qadir Mahmood, Sakar Abdulkarim Nidhamaddin

“…The results showed that there is an increase in the cost per quality-adjusted life year (QALY) gained with ophthalmic surgeries and DALY. …”
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The use of large language models in detecting Chinese ultrasound report errors by Yuqi Yan, Kai Wang, Bojian Feng, Jincao Yao, Tian Jiang, Zhiyan Jin, Yin Zheng, Yahan Zhou, Chen Chen, Lin Sui, Xiayi Chen, Yanhong Du, Jie Yang, Qianmeng Pan, Lingyan Zhou, Vicky Yang Wang, Ping Liang, Dong Xu

“…LLMs processed reports faster than the quickest radiologist (Claude 3.5 Sonnet: 13.2 s, GPT-4o: 15.0 s, radiologist: 42.0 s per report). This study demonstrates the potential of LLMs to enhance ultrasound report accuracy, outperforming human experts in certain aspects.…”
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Effect of Vigorous-Intensity Physical Activity on Incident Cognitive Impairment in High-Risk Hypertension. by Kazibwe, Richard, Schaich, Christopher L., Muhammad, Ahmad Imtiaz, Epiu, Isabella, Namutebi, Juliana H., Chevli, Parag A., Kazibwe, Joseph, Hughes, Timothy, Rikhi, Rishi R., Shapiro, Michael D., Yeboah, Joseph

“…Results: Participants in the high VPA category, compared with low VPA, experienced lower events rates (per 1000 person-years) of MCI (13.9 vs 19.7), probable dementia (6.3 vs 9.0), and MCI/probable dementia (18.5 vs 25.8). …”
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Psychosocial Burden in Parents Having Intellectually Disabled Children: A Hospital-based Study by Reena Kumari, Tapas Kumar Ray, Ananya Ray Laskar, Ritu Majumdar

“…These variables were a) per-capita monthly family income, b) socioeconomic status, c) study subjects’ occupation, and d) duration of disability. …”
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Impact of Pentoxifylline and Vitamin E on Ribavirin-Induced Haemolytic Anaemia in Chronic Hepatitis C Patients: An Egyptian Survey by M. Assem, M. Yousri

“…Both (ETR) and (SVR) were significantly higher in combined group than control group by both intention-to-treat analysis (71 versus 56%, P<.05 and 66 versus 49%, P<.05) and per-protocol analysis (85.5 versus 70.9%, P<.05 and 79.5 versus 62%, P<.05). …”
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Fetal Sacrococcygeal Teratoma: A Case Report of a Giant Tumor with an Excellent Outcome by Anna Kornete, Diana Bokucava, Natalija Vedmedovska

“… Sacrococcygeal teratoma (SCT) occurs in approximately 1 per 20,000–40,000 births and is the most frequently encountered fetal teratoma, with 75% of cases observed in female fetuses. …”
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Immediate Sequential Bilateral Cataract Surgery: A Systematic Review and Meta-Analysis by Line Kessel, Jens Andresen, Ditte Erngaard, Per Flesner, Britta Tendal, Jesper Hjortdal

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Optimizing polycrystalline diamond compact bit selection and drilling parameters for deviated wells in the Majnoon Field, Iraq by Ahmed N. Al-Dujaili, Maaly S. Asad, Najah Saeed, Nurbol Tileuberdi

“…The maximum weight on bit (WOB), Round per Minute (RPM), mud weight, and flow rate must be 25.45–73 klbs, 50-179.22, 10.5–10.8 ppg, and 662–940 gpm respectively, according to the drilling section. …”
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Wood Loss Assessment in Forest of Sal (Shorea robusta) by Heart Rot of Central Terai of Nepal by Shankar Tripathi, Yojana Adhikari

“…Before implementation of scientific forest management modality, the timber retained in stump per tree was found as 0.18 cubic feet.…”
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Spatial analysis of precipitation in Mazandaran province by ali mohammadpourzeidi, Bohloul Alijani, Mohammad Saligheh, MohammadSaleh Gerami

“…This range is also about 20 per cent of the province's total. About 65 percent of the total area of the province as well as the lack of a significant trend show.…”
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Nutrition and Reproductive Health: Sperm versus Erythrocyte Lipidomic Profile and ω-3 Intake by Gabriela Ruth Mendeluk, Mariano Isaac Cohen, Carla Ferreri, Chryssostomos Chatgilialoglu

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Effect of Scapular Intensive Training on Functional Recovery of Upper Extremities in Early Stroke by Min JIANG, Lun LUO, Fan BAI, Wenyuan SU, Chaomou HE, Tao XIANG

“…The control group were treated with conventional medical therapy and routine rehabilitation training, and the scapula reinforcement group increased scapular intensive training on the basis of the control group,40 minutes each time, once daily,7 days per week, continuous treatment for 4 weeks. Fugl-Meyer upper eExtremity motor function rating scale, Hong Kong version of hemiplegic upper extremity functional test (FTHUE-HK) scale and modified Barthel index (MBI) score were used to evaluate upper limb function and activities of daily living in patients with stroke.Results:After 4 weeks of treatment, Fugl-Meyer upper limb motor function scores, FTHUE-HK scale scores, and MBI index scores were significantly higher than those before treatment in both groups, with statistically significant differences (<italic>P</italic>&lt;0.05);and the scores of the evaluation scores of the scapular augmentation group were higher than those of the control group, with statistically significant differences (<italic>P</italic>&lt;0.05).Conclusion:The scapula reinforcement group can improve the upper extremity motor function and the activities of daily living in stroke patients.…”
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Incidence of neoplastic diseases in infants in Cienfuegos province from January 1988 to December 1998. by Luis Pedro Hernández Sosa, Sonia Pérez García, Mileny Acosta Fonseca

“…<br /><strong>Method: </strong>Descriptive- retrospective study with a universe that gathered all the patients less than 15 years of age who had a diagnosis of neoplasia in Cienfuegos province, Cuba in the period January1988 – December 1998 . The rate of incidence per 100 000 inhabitants aged less than 15 was obtained and the variables under study were identification data: age, sex, race, (classified as whites and no whites ), municipality of origin and type of tumour. …”
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Sensitivity Analysis of MTPA Control to Angle Errors for Synchronous Reluctance Machines by Martin Petrun, Jernej Černelič

“…This study investigated the sensitivity of maximum torque per ampere (MTPA) control in synchronous reluctance machines (SynRMs) to angle errors, examining specifically how deviations in the reference control trajectory affected performance. …”
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Waiting Behavior and Arousal in Different Levels of Crowd Density: A Psychological Experiment with a “Tiny Box” by Mira Beermann, Anna Sieben

“…Crowd density, defined as persons per square meter, is a basic measuring unit for describing and analyzing crowd dynamics and for planning pedestrian infrastructure. …”
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The specific heat loss combined with the thermoelastic effect for an experimental analysis of the mean stress influence on axial fatigue of stainless steel plain specimens by G. Meneghetti, M. Ricotta, B. Atzori

“…The energy dissipated to the surroundings as heat in a unit volume of material per cycle, Q, was recently proposed by the authors as fatigue damage index and it was successfully applied to correlate fatigue data obtained by carrying out fully reversed stress- and strain-controlled fatigue tests on AISI 304L stainless steel plain and notched specimens. …”
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APICAL MICROLEAKAGE OF VARIOUS BIOMATERIALS IN SIMULATED IMMATURE APICES by Fatih Tulumbacı, Volkan Arıkan, Aylin Akbay Oba, İşıl Sönmez Şaroğlu

“…In order to simulate immature teeth, 57 bovine maxillary incisor roots were prepared and randomly divided into three groups (n = 15 per group). The remaining teeth were included in negative (n=6) and positive (n=6) control groups. …”
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