Improving genome assemblies by sequencing PCR products with PacBio by Xiaojing Zhang, Karen W. Davenport, Wei Gu, Hajnalka E. Daligault, A. Christine Munk, Hazuki Tashima, Krista Reitenga, Lance D. Green, Cliff S. Han

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Extraction of high-molecular-weight genomic DNA for long-read sequencing of single molecules by Baptiste Mayjonade, Jérôme Gouzy, Cécile Donnadieu, Nicolas Pouilly, William Marande, Caroline Callot, Nicolas Langlade, Stéphane Muños

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Comparative analysis of Illumina, PacBio, and nanopore for 16S rRNA gene sequencing of rabbit’s gut microbiota by Iliyass Biada, María Antonia Santacreu, Oscar González-Recio, Noelia Ibáñez-Escriche

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Oar_Finn: the genome assembly and annotation of an exceptionally fertile Finnsheep (Ovis aries) Ewe by Juha Kantanen, Melak Weldenegodguad, Kisun Pokharel

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Insights into the length and breadth of methodologies harnessed to study human telomeres by Tiernan Coulter, Claire Hill, Amy Jayne McKnight

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Complete genome sequence data of Xylella fastidiosa subspecies multiplex ST88 and ST89 indicate distinct introductions in FranceNCBINCBI by Amandine Cunty, Jessica Dittmer, Déborah Merda, Bruno Legendre, Benoit Remenant, Yannick Blanchard, Sophie Cesbron, Marie-Agnès Jacques, Pascal Gentit, Anne-Laure Boutigny

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First fine mapping of a strain of Rhizoctonia Solani AG-3, causing tobacco target spot by Pan Ma, Mengjuan Qiu, Rubing Xu, Zhao Wang, Tom Hsiang, Junbin Huang, Lu Zheng, Yanyan Li

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Chromosome-scale genome assembly and annotation of two geographically distinct strains of malaria vector Anopheles albimanus by Dieunel Derilus, Gareth D. Weedall, Michael W. Vandewege, Dhwani Batra, Mili Sheth, Lori A. Rowe, Ananias A. Escalante, Audrey Lenhart, Lucy Mackenzie Impoinvil

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Seq2scFv: a toolkit for the comprehensive analysis of display libraries from long-read sequencing platforms by Marianne Bachmann Salvy, Luca Santuari, Emanuel Schmid-Siegert, Nikolaos Lykoskoufis, Ioannis Xenarios, Bulak Arpat

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Discovery of Antibodies Against Endemic Coronaviruses with NGS-Based Human Fab Phage Display Platform by Oscar Chi-Chien Pan, Sean Miller, Ruchin Patel, Shreya Mukhopadhyay, Giancarlo Sarullo, Gwenny Go, Jennifer Galli, Jamie Hessels, Barbara Schlingmann-Molina, Emmanuel Ndashimye, Zhiyun Wen, Christopher Warren, Eberhard Durr, Lan Zhang, Kalpit A. Vora, Arthur Fridman, Zhifeng Chen

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High-Quality Complete Genome Sequence of Xanthomonas phaseoli pv. dieffenbachiae Outbreak Strain D182: The Causative Agent of Anthurium Bacterial Blight in Hawaii by Shefali Dobhal, Shu-Cheng Chuang, Dario Arizala, Lisa M. Keith, Anne M. Alvarez, Mohammad Arif

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High-throughput PacBio library preparation and sequencing techniques for genomic DNA and TNA by Meghan L. Bentz, Cristina Clines, Jasmine Padilla, Christopher Horn, Justin Lee, Mili Sheth

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Characterization of a novel AEL allele harboring a c.28 + 5G>A mutation on the ABO*A2.01 background: a study utilizing PacBio third-generation sequencing and functional assays by Lin-Nan Shao, Wen-Qian Song, Lu Zhou, Ling-Zi Pan, Ying Duan, Nan Xiao, Shi-Hang Zhou, Xiao-Hua Liang

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Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding? by Robert Lücking, M. Catherine Aime, Barbara Robbertse, Andrew N. Miller, Hiran A. Ariyawansa, Takayuki Aoki, Gianluigi Cardinali, Pedro W. Crous, Irina S. Druzhinina, David M. Geiser, David L. Hawksworth, Kevin D. Hyde, Laszlo Irinyi, Rajesh Jeewon, Peter R. Johnston, Paul M. Kirk, Elaine Malosso, Tom W. May, Wieland Meyer, Maarja Öpik, Vincent Robert, Marc Stadler, Marco Thines, Duong Vu, Andrey M. Yurkov, Ning Zhang, Conrad L. Schoch

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The first complete mitochondrial genome of sesame (Sesamum indicum L.) by Mingcheng Wang, Rui Li, Xuchen Yang

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Haplotype-resolved and gap-free genome of a floating aquatic plant from the Oryzeae tribe, Hygroryza aristata by Li-Yao Yang, Li-Kun Huang, Jin-Bin Lin, Cun-Jing Xu, Wei-Qi Tang, Bi-Guang Huang

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Reference genome of the leopard seal (Hydrurga leptonyx), a Southern Ocean apex predator by J. Canitz, S. S. Kienle, S. S. Kienle, K. van der Linde, R. Borras-Chavez, R. Borras-Chavez, E. S. Sperou, A. Leahy, A. Leahy, S. Rivera, S. Rivera, M. Autenrieth, J. I. Hoffman, J. I. Hoffman, J. I. Hoffman, J. I. Hoffman, J. I. Hoffman, C. A. Bonin

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Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation by Paul Coupland, Tamir Chandra, Mike Quail, Wolf Reik, Harold Swerdlow

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Residual DNA impurities in AAV vectors—nature and transcription by Hsin-I Jen, Patrick Wilkinson, Xiaohui Lu, Wei Zhang

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An epithelial cell culture model for sturgeon integument responds sensitively to 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure by Sumi Nechat, Melina Shadi, Andrea D. Schreier, Nann A. Fangue, John P. Sundberg, Robert H. Rice

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