Relation among zootechnical performance, biochemical indicators, water quality, and small invertebrates (zooplankton) abundance reared in biofloc-supplemented systems by Ahmed Said Al-Souti, Marwa M. Zaher, Amr M. Helal, Dina T. Meshhal, Mansour D. H., Afify D. G. Al-Afify, Mohamed M. Rafaey, Abeer El-Saharty, Ehab El-Haroun, Marian G. Nassif, Abdallah Tageldein Mansour, Abdallah Tageldein Mansour, Mohamed Ashour

“…Compared to the control group (T0, zero biofloc water supplementation), the influence of five biofloc supplementation levels was applied as follows: 14.2, 28.4, 42.6, 56.8, and 71 g L−1 (T1, T2, T3, T4, and T5, respectively). The biofloc was prepared in an external fermentor fiberglass tank (300 L) and added to the fish tanks to keep the biofloc levels constant during the experiment. …”
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In vitro anticancer effects of frankincense and its nanoemulsions for enhanced cancer cell targeting by Rayya A. Al-Balushi, Aiswarya Chaudhuri, Raghuram Kandimalla, Ashanul Haque, Khalaf M. Alenezi, Mohd. Saeed, Mohammad Changez, Thuraya Al Harthy, Mohammed Al Hinaai, Samra Siddiqui, Ashish Kumar Agrawal, Farrukh Aqil, Farrukh Aqil

“…FO-based nanoemulsions (FO-NEs) were prepared and optimized using a 3-factor, 3-level Box-Behnken Design (BBD), with 10% FO (v/v), 40% surfactant (cremophor EL), and co-surfactant (Transcutol P). …”
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Diagnostics of lung cancer by fragmentated blood circulating cell-free DNA based on machine learning methods by Ivan O. Meshkov, Alexander P. Koturgin, Pavel V. Ershov, Liubov A. Safonova, Julia A. Remizova, Valentina V. Maksyutina, Ekaterina D. Maralova, Vasilisa A. Astafieva, Alexey A. Ivashechkin, Boris D. Ignatiev, Antonida V. Makhotenko, Ekaterina A. Snigir, Valentin V. Makarov, Vladimir S. Yudin, Anton A. Keskinov, Sergey M. Yudin, Anna S. Makarova, Veronika I. Skvortsova

“…The aim of this work is to create a method for processing next-generation sequencing (NGS) data and an interpretable binary classification model (CM), which analyzed cfDNA fragmentation features for distinguishing healthy subjects and subjects with LC.Methods148 healthy subjects and 138 subjects with LC were included in the study. cfDNA fractions, isolated from blood plasma biospecimens, were used for DNA libraries preparations and NGS on the NovaSeq 6,000 Illumina system with a coverage of 100 million reads/sample. …”
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Reinforcing cancer immunotherapy with engineered porous hollow mycobacterium tuberculosis loaded with tumor neoantigens by Jie Jiang, Feng-Ying Huang, Shu-Zhen Dai, Guang-Hong Tan, Ming-Hui Chen, Hengyu Chen, Wu-Ping Zheng, Ri-Hong Wu, Weijing Xie

“…Therefore, we suggest using MTb as a carrier to enhance APC phagocytosis of neoantigens, thereby promoting antigen cross-presentation.Methods The successful preparation of the MTb carrier (phMTb) was confirmed through electron and confocal microscopy. …”
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Study protocol: type II hybrid effectiveness-implementation study of routine functional status screening in VA primary care by Francesca M. Nicosia, Kara Zamora, LauraEllen Ashcraft, Gregory Krautner, Marybeth Groot, Bruce Kinosian, Cathy C. Schubert, Sumedha Chhatre, Helene Moriarty, Orna Intrator, Andrea Wershof Schwartz, Ariela R. Orkaby, Jason Prigge, Rebecca T. Brown

“…Abstract Background Maintaining functional status, defined as the ability to perform daily activities such as bathing, dressing, and preparing meals, is central to older adults’ quality of life, health, and ability to remain independent. …”
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A phase 1b clinical trial to determine the safety, tolerability and immunogenicity of simian adenovirus and poxvirus vectored vaccines against Mycobacterium avium complex subspecie... by Jeremy Sanderson, Jeremy Aboagye, Rebecca Makinson, Katerina Rapi, Samuel Provstgaard-Morys, Lisa Stockdale, Alison Lawrie, Isabelle Lanigan, Nishat Halim, Abdel Douiri, Emily Greenlay, Rayka Malek, Emma Gray, Lindsey West, Fatima El Oulidi, Paul Ian Cross, Michael Stallibrass, Sarah C. Gilbert, Adrian V.S. Hill, Katie J. Ewer

“…The Sponsor was involved in protocol development, trial conduct, including data monitoring and analysis, and the preparation of this manuscript in line with the Medicines for Human Use (Clinical Trials) Regulations 2004 and amendments.…”
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Whole genomic analysis uncovers high genetic diversity of rifampicin-resistant Mycobacterium tuberculosis strains in Botswana by Tuelo Mogashoa, Tuelo Mogashoa, Johannes Loubser, Ontlametse T. Choga, Ontlametse T. Choga, Justice Tresor Ngom, Wonderful T. Choga, Wonderful T. Choga, Mpaphi B. Mbulawa, Tuduetso Molefi, One Stephen, Topo Makhondo, Kedumetse Seru, Patience Motshosi, Boitumelo Zuze, Joseph Makhema, Rosemary M. Musonda, Dimpho Otukile, Chawangwa Modongo, Botshelo T. Kgwaadira, Keabetswe Fane, Simani Gaseitsiwe, Rob M. Warren, Sikhulile Moyo, Sikhulile Moyo, Sikhulile Moyo, Sikhulile Moyo, Anzaan Dippenaar, Elizabeth M. Streicher

“…Genomic DNA was extracted using the cetyltrimethylammonium bromide (CTAB) method. Library preparation was performed using the Illumina DNA prep kit following the manufacturer's instructions. …”
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Influence of diet nutrient density and a multicarbohydrase containing α-galactosidase on performance, carcass characteristics, breast myopathies, and nutrient utilization of broile... by J.P. Gulizia, J.I. Vargas, S.M. Bonilla, J.R. Hernandez, L.M. Almeida, P. Soster, S. Thuekeaw, B. Hutson, S. Llamas-Moya, T. Duong, W.J. Pacheco

“…Additional treatments were prepared by supplementing 0.2 g CAG per kg feed to the basal diets. …”
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Development and use of a fully automatic clinical intelligent milk dispenser (一种全自动临床智能配奶机的研制与使用) by ZHANG Rongbang (张榕邦), OUYANG Li (欧阳丽), JIN Mu (金募), LIU Lingzhi (刘羚芷), LIN Yan (林妍), ZHANG Zhiyuan (张秩源)

“…To design a new type of fully automatic intelligent milk dispenser that is different from traditional milk preparation methods, which is based on Internet technology and combined with artificial intelligence. …”
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Therapeutic potential of Curcuma longa against monkeypox: antioxidant, anti-inflammatory, and computational insights by Farouk Boudou, Amal Belakredar, Ahcen Keziz, Linda Aissani, Huda Alsaeedi, David Cronu, Mikhael Bechelany, Mikhael Bechelany, Ahmed Barhoum

“…Molecular docking and molecular dynamics (MD) simulations were performed to evaluate the interactions between curcuminoids and monkeypox virus proteins.ResultsThe aqueous extract of C. longa was prepared via decoction, yielding 7.80% ± 0.81% extract with curcumin as the predominant compound (36.33%). …”
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Nursing cooperation of robot-assisted laparoscopic partial nephrectomy through retroperitoneal approach (经后腹腔入路达芬奇机器人肾部分切除术的护理配合) by ZHANG Han (张含), LIN Li (林丽), DONG Kui (董奎), LIU Qing (刘青)

“…Perfect preoperative nursing preparation and intraoperative nursing cooperation, especially special postural nursing play an important role in ensuring the smooth operation and preventing surgical complications. …”
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Detection of cervical precancerous lesions and cancer by small-scale RT-qPCR analysis of oppositely deregulated mRNAs pairs in cytological smears by Anastasia A. Artyukh, Mikhail K. Ivanov, Mikhail K. Ivanov, Sergei E. Titov, Sergei E. Titov, Victoria V. Dzyubenko, Sergey E. Krasilnikov, Sergey E. Krasilnikov, Anastasia O. Shumeikina, Anastasia O. Shumeikina, Anastasia O. Shumeikina, Nikita A. Afanasev, Anastasia V. Malek, Sergei A. Glushkov, Eduard F. Agletdinov

“…The method is suitable for the analysis of cervical cytological smears prepared by a routine method. Further clinical validation is necessary to clarify its clinical potential.…”
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Neuropilin1-dependent paracrine signaling of cancer cells mediated by miRNA exosomal cargo by Claudia Palazzo, Roberta Mastrantonio, Noemi Gioelli, Erika Testa, Francesco Recco, Donatella Lucchetti, Giulia Villari, Alessio D’Alessio, Alessandro Sgambato, Flavio Mignone, Guido Serini, Maria Teresa Viscomi, Luca Tamagnone

“…The quality of exosome-enriched preparations was validated by the presence of specific markers in western blotting, as well as by light scattering and nanoparticle tracking analysis. …”
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Construction and application of the three-level storage information system in the management of low-cost surgical consumables (手术室低值耗材三级库信息化管理模式的构建及应用)... by XU Ying (许瑛), YANG Lijun (杨利君), MENG Yuehua (孟月华), LIU Lin (柳琳), ZHANG Lianen (张怜恩)

“…Results Time spent in preparing and supplying low-cost surgical consumables and interval time between two operations were reduced after implementation of three-level storage information system (P＜0. 05). …”
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Supported biopsychosocial self-management for back-related leg pain: a randomized feasibility study integrating a whole person perspective by Brent Leininger, Roni Evans, Carol M. Greco, Linda Hanson, Craig Schulz, Michael Schneider, John Connett, Francis Keefe, Ronald M. Glick, Gert Bronfort

“…This feasibility study was done in preparation for a full-scale trial comparing a whole-person supported self-management intervention to medical care for chronic BRLP. …”
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Validation study of synthetic models for esophageal atresia with tracheoesophageal fistula (EA/TEF): A simulation-based training in pediatric surgery by Zahradníková Petra, Hnilicová Silvia, Lindák Martin, Pechanová Rebeka, Števková Dominika, Vitovič Pavol, Laurovičová Miroslava, Horn František, Tvrdoň Tomáš, Babala Jozef

“…Conclusion: Simulation training is essential for teaching MIS in paediatric surgery, improving skills, and should be part of specialisation preparation. Synthetic EA/TEF models received high ratings as effective training tools for thoracoscopic EA/TEF training. …”
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Study on the correlation between microstructural evolution and hydrogen storage properties in hyper-eutectic Mg-xNi (x = 15, 20, 25) alloys by So Jin Jung, Seok Su Sohn, Da Hye Lee, Won-Seok Ko, Tae Jun Ha, Ryun-Ho Kwak, Byeong-Chan Suh, Young Min Kim, Hyung-Ki Park

“…Mg-xNi (x = 15, 20, 25 (at%)) alloys were prepared using vacuum induction melting. These alloys consisted of a primary Mg2Ni phase and an Mg–Mg2Ni lamellar structure, with the proportion of the primary Mg2Ni phase increasing as the Ni content rose. …”
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Rat models of postintracerebral hemorrhage pneumonia induced by nasal inoculation with Klebsiella pneumoniae or intratracheal inoculation with LPS by Ruihua Wang, Changlian Gan, Rui Mao, Yang Chen, Ru Yan, Geng Li, Tianqin Xiong, Jianwen Guo

“…Bronchoalveolar lavage fluid was collected for leukocyte counting, and supernatant was prepared to measure MPO activity. Ileum was isolated for immunofluorescence staining for tight junction proteins ZO-1 and γδ TCRs/IL-17A and for Alcian blue–nuclear fast red staining of acidic mucins. …”
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Single Dose of Attenuated Vaccinia Viruses Expressing H5 Hemagglutinin Affords Rapid and Long-Term Protection Against Lethal Infection with Highly Pathogenic Avian Influenza A H5N1... by Fumihiko Yasui, Keisuke Munekata, Tomoko Fujiyuki, Takeshi Kuraishi, Kenzaburo Yamaji, Tomoko Honda, Sumiko Gomi, Misako Yoneda, Takahiro Sanada, Koji Ishii, Yoshihiro Sakoda, Hiroshi Kida, Shosaku Hattori, Chieko Kai, Michinori Kohara

“…Background/Objectives: In preparation for a potential pandemic caused by the H5N1 highly pathogenic avian influenza (HPAI) virus, pre-pandemic vaccines against several viral clades have been developed and stocked worldwide. …”
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Shared genetic risk and causal associations between Post-traumatic stress disorder and migraine with antithrombotic agents and other medications by Charlotte K. Bainomugisa, Dagmar Bruenig, Heidi G. Sutherland, Lyn R. Griffiths, Dale R. Nyholt, Divya Mehta

“…Cross-disorder genetic analyses were applied to investigate the genetic overlap and causal associations using GWAS summary statistics of PTSD (n = 214408), migraine (n = 873341) and 23 medication use traits (n = 78808–305913) including anti-depressants, anti-migraine preparations and beta-blocking agents.Across the entire genome, anti-thrombotic agents had a significant and negative genetic correlation with PTSD (rG = −0.2, PFDR = 0.032) and a positive genetic correlation with migraine (rG = 0.26, PFDR = 2.23 x 10−8). …”
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