In vivo brain delivery of BBB-enabled iduronate 2-sulfatase in rats by Will J. Costain, Arsalan S. Haqqani, Greg Hussack, Henk van Faassen, Etienne Lessard, Binbing Ling, Eric Brunette, Dao Ly, Hung Fang, Jennyfer Bultinck, Steven Geysens, Gwenda Pynaert, Kathleen Piens, Stefan Ryckaert, Franck Fudalej, Wouter Vervecken, Danica Stanimirovic

“…Methods: Single-domain antibody (sdAb)-enzyme fusion protein constructs were prepared in Yarrowia lipolytica. sdAb affinity and BBB permeability were characterized using SPR and an in vitro rodent BBB assay, respectively. …”
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Effects of Eleclazine (GS6615) on the proarrhythmic electrophysiological changes induced by myocardial stretch by Francisco J. Chorro, Francisco J. Chorro, Francisco J. Chorro, Luis Such-Miquel, Luis Such-Miquel, Samuel Cuñat, Oscar Arias-Mutis, Oscar Arias-Mutis, Patricia Genovés, Patricia Genovés, Manuel Zarzoso, Manuel Zarzoso, Antonio Alberola, Antonio Alberola, Luis Such-Belenguer, Luis Such-Belenguer, Irene Del Canto, Irene Del Canto

“…The aim of this study was to investigate the electrophysiological modifications induced by eleclazine basally and during acute ventricular stretch.MethodsLeft ventricular stretch was induced at baseline and during perfusion with eleclazine in 26 Langendorff rabbit heart preparations. Programmed stimulation and high-resolution mapping techniques were applied using multiple epicardial electrodes.ResultsAt baseline, both the ventricular refractory period measured at a fixed cycle length (250 m) and its surrogate obtained during ventricular fibrillation (VF) decreased significantly during stretch (baseline 128 ± 15 vs. stretch 110 ± 14 m; n = 15; p < 0.001, and baseline 52 ± 13 vs. stretch 44 ± 9 m; n = 11; p < 0.05), while the VF dominant frequency (DF) increased significantly (DF baseline 13 ± 3 vs. stretch 17 ± 5Hz; n = 11; p < 0.01). …”
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One-Step Solid Extraction for Simultaneous Determination of Eleven Commonly Used Anticancer Drugs and One Active Metabolite in Human Plasma by HPLC-MS/MS by Shouhong Gao, Zhengbo Tao, Jingya Zhou, Zhipeng Wang, Yunlei Yun, Mingming Li, Feng Zhang, Wansheng Chen, Yejun Miao

“…One-step solid-phase extraction was successfully applied using an Ostro sample preparation 96-well plate for plasma samples pretreated with acetonitrile containing 0.1% formic acid. …”
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A randomized, double-blind, Phase 1, single- and multiple-dose placebo-controlled study of the safety and pharmacokinetics of IN-006, an inhaled antibody treatment for COVID-19 in... by Thomas R. Moench, Lakshmi Botta, Brian Farrer, Jason D. Lickliter, Hyunah Kang, Yoona Park, Cheolmin Kim, Marshall Hoke, Miles Brennan, Morgan D. McSweeney, Zachary Richardson, John B. Whelan, Jong Moon Cho, Soo Young Lee, Frances Faurot, Jeff Hutchins, Samuel K. Lai

“…Study staff and participants were blinded to treatment assignment, except for pharmacy staff preparing the study drug. The ratio of active:placebo randomization to each cohort was set at 3:1. …”
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Evaluation of host immune responses to Mycobacteriophage Fionnbharth by route of delivery by Thomas Smytheman, Tiffany Pecor, Dana E. Miller, Debora Ferede, Suhavi Kaur, Matthew H. Harband, Hazem F. M. Abdelaal, Carlos A. Guerrero-Bustamante, Krista G. Freeman, Whitney E. Harrington, Lisa M. Frenkel, Graham F. Hatfull, Rhea N. Coler, Sasha E. Larsen

“…Furthermore, we found that highly-purified phage preparations did not activate mouse or human derived toll like receptor (TLR) 4 or TLR9 in HEKblue reporter assays. …”
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Transauricular vagus nerve stimulation in preventing post-traumatic stress disorder in emergency trauma surgery patients in China: a study protocol for a multicenter, double-blind,... by Jun Zhang, Xin Yu, Gang Chen, Yu Li, Qi-Hong Shen, Xinru Lin, Tieshuai Liu, Yunyun Yu, Jingwen Liang

“…The initial intervention will begin once informed consent is obtained and randomisation is completed in the preoperative preparation room, continuing until the surgery is finished. …”
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Double degree programs and education for sustainable development implementation: Experience of Ukrainian and UK universities cooperation in wartime by Iryna Skliar, Mykola Kostel’, Olena Melnyk, Kseniia Bliumska-Danko

“…Acknowledgment This contribution is prepared with the support and funding under Researchers at Risk Fellowships Programme led by the British Academy in partnership with the Academy of Medical Sciences, the Royal Academy of Engineering, the Royal Society and Council for At-Risks Academics (CARA), and in the framework of the realization the Erasmus+ KA 2 CBHE project “Universities-Communities: Strengthening Cooperation” (Grant Agreement 101083077), and Jean Monnet Centre of Excellence “EU Studies of Social Innovations in Education” (ESSIE) – 101085552 – ERASMUS-JMO-2022-COE.The European Commission’s support for the production of this publication does not constitute an endorsement of the contents that reflect the views only of the authors, and the Commission cannot be held responsible for any use that may be made of the information contained therein.…”
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Immunogenicity in humans of a transdermal multipeptide melanoma vaccine administered with or without a TLR7 agonist by Craig L Slingluff, Gina R Petroni, Walter C Olson, Kimberly A Chianese-bullock, Donna Deacon, Mark Smolkin, William W Grosh, Max O Meneveau, Kevin T Lynch, Elise P Salerno, Elizabeth Woodson, James W Patterson

“…We hypothesized that: (1) applying melanoma-derived peptides topically, in proximity to LC, could be immunogenic and safe, with low vaccine-site toxicity and (2) topical toll-like receptor 7 (TLR7) agonist would increase immunogenicity of the peptide vaccine.Methods Twelve melanoma peptides plus a tetanus helper peptide were combined with granulocyte macrophage colony stimulating factor (GM-CSF) and were administered topically on days 1, 8, and 15, to 28 patients randomized to one of four adjuvant preparations: (1) incomplete Freund’s adjuvant (IFA); (2) IFA plus a TLR7 agonist (imiquimod) administered on days 0, 7, 14; (3) dimethyl sulfoxide (DMSO) or (4) DMSO+ imiquimod administered on day 0, 7, 14. …”
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CD44-Receptors-Mediated Multiprong Targeting Strategy Against Breast Cancer and Tumor-Associated Macrophages: Design, Optimization, Characterization, and Cytologic Evaluation by Hussain Z, Abdulrahim Abdul Moti L, Jagal J, Thu HE, Khan S, Kazi M

“…In this research, we aimed to design a multiprong targeting strategy for concurrent action against different phenotypes of BC (MCF-7 and SK-BR-3) and tumor-associated macrophages (TAMs) for relapse-free treatment of BC.Methods: Paclitaxel (PTX) and tamoxifen (TMX) co-loaded chitosan (CS) nanoparticles (NPs) were prepared using the ionic-gelation method and optimized using the Design Expert® software by controlling different material attributes. …”
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Socio-demographic portrait of the applicant and the main motives for choosing the "Therapy and rehabilitation" speciality by S.V. Zakharov, O.B. Nekhanevych, O.O. Rusakova, O.V. Smolianova

“…Most applicants entering the speciality 227 "Therapy and rehabilitation" are goal-oriented, highly motivated, and prepared for rigorous study, which is the key to developing into competent and competitive specialists.…”
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Biodesulfurization of high-sulfur oil from the Karazhanbas field of Kazakhstan with deep eutectic solvents by A.O. Akimbek, G.A. Jamalova, A.K. Yernazarova, G.K. Kaiyrmanova, B.K. Yelikbayev, M.C. Pagano, A.G. Zazybin, Kh. S. Rafikova

“…The research methodology was based on the application of the Koch methods to determine the total number of microorganisms; light microscopy – for the study of microbiological preparations; genetic identification of bacteria based on the analysis of the nucleotide sequence of a fragment of the 16S rRNA gene; synthesis of deep eutectic solvents; testing of isolated bacteria – for sensitivity to Cr (VI), for the ability of microorganisms to use hydrocarbons of high-sulfur oil, for activity in sulfur-containing crude oil, for determination of the mass fraction of sulfur. …”
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Evaluasi Kinerja pelaksanaan Anggaran Berbasis Fuzzy Inference System by Sukarna Sukarna, Irman Hermadi, Yani Nurhadryani

“…The results of this studied are monitoring and evaluation of the web-based work unit in the form of real performance status as a basis for preparing recommendations in  order to improve budget performance.  …”
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Experiences of midwifery students and graduates in Somalia: evidence from qualitative data by Hawa Abdullahi, Asia Mohamed Mohamud, Maryan Abdulkadir Ahmed, Mohamed Ahmed Omar, Abdirisak A. Dalmar, Hannah Tappis, Shatha Elnakib

“…In terms of perceived preparedness, graduates felt well prepared for their role but identified gaps in abortion care, neonatal resuscitation, and usage of basic ultrasound.ConclusionThe challenges faced by midwifery students and newly graduated midwives have direct implications for the health outcomes of mothers and infants in conflict-affected settings. …”
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The Identification of Molecular Ploidy Status of Abnormal Pronuclear Zygotes Reveals a Significant Number of Euploid Blastocysts Available for Conception by Blair R. McCallie, Mary E. Haywood, Lauren N. Henry, Rachel M. Lee, William B. Schoolcraft, Mandy G. Katz-Jaffe

“…Biopsied trophectoderm cells were then lysed, and the isolated DNA was whole-genome amplified followed by library preparation. Next-generation sequencing was performed for PGT-A, and excess whole-genome amplified DNA was utilized for single nucleotide polymorphism beadchip array analysis. …”
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Value Propositions for Digital Shared Medication Plans to Boost Patient–Health Care Professional Partnerships: Co-Design Study by Benjamin Bugnon, Francesca Bosisio, Alain Kaufmann, Pascal Bonnabry, Antoine Geissbuhler, Christian von Plessen

“…The experience-based co-design had 4 phases: capturing, understanding, and improving experiences, followed by preparing recommendations and next steps. A qualitative, participatory methodology was used to iteratively explore collaborative medication management experiences and identify barriers and enabling mechanisms, including technology. …”
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Buffalo whey proteins as innovative raw material for nanoparticles building by Pablo A. Solis Cizek, Gustavo A. Crudeli, Darío Spelzini, Franco E. Vasile

“…An aqueous suspension containing BWPC (0.1% w/v) and GA (0.05% w/v) was prepared for this purpose. For comparative purposes, the behavior of mixtures containing cow whey (WPC) and GA, and the single components were also studied. …”
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Prediksi Stok dan Pengaturan Tata Letak Barang Menggunakan Kombinasi Algoritma Triple Exponential Smoothing dan FP-Growth by Kristoko Dwi Hartomo, Sri Yulianto Prasetyo, Rahmat Abadi Suharjo

“…By overcoming it, the purpose of the research is to regulate the procedures for goods and optimize the preparation of stock items. In this study using processed sales data. …”
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Linear Programming as a Tool for Managing the Training Process of Esports Teams by Oksana Shynkaruk, Nataliia Byshevets, Alla Aloshyna, Olena Iakovenko, Kostiantyn Serhiienko, Valerii Pinchuk, Oleksandr Petryk, Ivan Lut

“…Variant 2 facilitates the investigation of different scenarios for planning the training process of esports teams, demonstrating how the distribution of time across training types changes depending on set goals and preparation phases. Conclusions. Based on the proposed algorithm, linear programming variants were developed, successfully addressing the task of automating the planning of esports team training. …”
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Novel Nanozyme-Based Multicomponent in situ Hydrogels with Antibacterial, Hypoxia-Relieving and Proliferative Properties for Promoting Gastrostomy Tube Tract Maturation by Xiao F, Yan B, Yuan T, He Y, Zhang X, He X, Peng W, Xu Y, Cao J

“…In situ hydrogels were prepared by mixing the HP/ alginate solution, and the n-MO solution containing Ca2+. …”
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After neonatal care, what next? A qualitative study of mothers’ post-discharge experiences after premature birth in Kenya by Justinah Maluni, Dorothy Oluoch, Sassy Molyneux, Mwanamvua Boga, Caroline Jones, Florence Murila, Mike English, Sue Ziebland, Lisa Hinton

“…This study explored the post-discharge experiences of mothers of premature babies with the aim of identifying their needs and suggests strategies to strengthen and support their discharge preparation to care for their premature baby at home, and to and reduce mortality and readmission rates. …”
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