Effect of SIS3 on Extracellular Matrix Remodeling and Repair in a Lipopolysaccharide-Induced ARDS Rat Model by Qiong Liang, Qiqing Lin, Yueyong Li, Weigui Luo, Xia Huang, Yujie Jiang, Chunyan Qin, Jin Nong, Xiang Chen, Suren Rao Sooranna, Liao Pinhu

“…A selective inhibitor of Smad3 (SIS3) was intraperitoneally injected into the disease model, while phosphate-buffered saline (PBS) was used in the control group. Animal tissues were then evaluated using histological analysis, immunohistochemistry, RT-qPCR, ELISA, and western blotting. …”
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Synthesis and characterization of a magnetic bacterial cellulose-chitosan nanocomposite and evaluation of its applicability for osteogenesis by Nahid Rezazadeh, Effat Alizadeh, Somaieh Soltani, Soodabeh Davaran, Neda Esfandiari

“…The biodegradability of the scaffolds was assessed using PBS for 8 weeks at 37°C. The cytotoxicity and osteogenic differentiation of the nanocomposite were studied using human adipose-derived mesenchymal stem cells (ADSCs) and alizarin red staining. …”
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The Mycoplasma hyopneumoniae protein Mhp274 elicits mucosal and systemic immune responses in mice by Mengqi Xie, Zhongshun Huang, Yun Zhang, Yujie Gan, Huiying Li, Dan Li, Honglei Ding

“…Fifty-two six-week-old SPF female BALB/c mice were divided into five groups: a commercial inactivated vaccine-immunized group, three recombinant protein-inoculated groups, and a PBS-treated group. The physical parameters and body weights of the mice were observed during the experiment. …”
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Evaluation of tick salivary and midgut extracellular vesicles as anti-tick vaccines in White-tailed deer (Odocoileus virginianus) by Julia Gonzalez, Cristina Harvey, Cárita de Souza Ribeiro-Silva, Brenda Leal-Galvan, Kelly A. Persinger, Pia U. Olafson, Tammi L. Johnson, Adela Oliva Chavez

“…Two control deer were injected with adjuvant and PBS only. On day 58, WTD were infested with 100 A. americanum nymphs, 50 females, and 50 males that were allowed to feed to repletion. …”
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Adipose-Derived Mesenchymal Stem Cells Migrate and Rescue RPE in the Setting of Oxidative Stress by Aya Barzelay, Shira Weisthal Algor, Anat Niztan, Sebastian Katz, Moshe Benhamou, Itay Nakdimon, Noam Azmon, Sandy Gozlan, Daphna Mezad-Koursh, Meira Neudorfer, Michaella Goldstein, Benjamin Meilik, Anat Loewenstein, Adiel Barak

“…Under such conditions, ASC-treated mice showed preservation of nuclear layers in the outer nuclear layer and stronger staining of RPE and photoreceptor layer, compared to PBS-treated mice. Taken together, our results indicate that ASCs are able to home in on damaged RPE cells and protect against damage to the RPE and PR layers caused by oxidative stress. …”
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Solid Self-Microemulsifying Drug Delivery System for Improved Oral Bioavailability of Relugolix: Preparation and Evaluation by Li ZL, Deng GX, Fang CZ, Zhao YQ, Yuan J, Chen L, Zhong HJ, Guo F

“…In phosphate buffered saline (PBS) (pH 6.8) release medium containing 1% tween 80, the vitro release studies showed 86% cumulative drug release for RLGL-S-SMEDDS and 3.6% cumulative drug release for RLGL suspensions. …”
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Stability and Efficacy of Live-Attenuated Vibrio harveyi Vaccines Under Different Storage Conditions in Zebrafish (Danio rerio) Models by Mohd Hafiz Ngoo Muhamad-Sofie, Aslah Mohamad, Mohamad Azzam-Sayuti, Mohammad Noor Azmai Amal, Mohd Zamri-Saad, Md Shirajum Monir, Ina Salwany Md Yasin

“…Groups 1, 2, and 3 were intraperitoneally injected with different LAVh vaccine storage (fresh, stale, and freeze-dried, respectively), while Group 4 received 0.01 M phosphate-buffered saline (PBS) and served as the unvaccinated control. Fish were monitored for 21 days post-vaccination for safety, stability, efficacy, and antibody analysis. …”
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Human Umbilical Cord-Derived Mesenchymal Stem Cell Therapy Ameliorates Nonalcoholic Fatty Liver Disease in Obese Type 2 Diabetic Mice by Bing Li, Yu Cheng, Songyan Yu, Li Zang, Yaqi Yin, Jiejie Liu, Lin Zhang, Yiming Mu

“…Thirty-week-old male C57BL/6 db/db mice were infused with human UC-MSCs or phosphate-buffered saline (PBS) via the tail vein once a week for six weeks. …”
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Effects of a New Nano-Silver Fluoride-Containing Dentifrice on Demineralization of Enamel and Streptococcus mutans Adhesion and Acidogenicity by Joás Araújo Teixeira, Amitis Vieira Costa e Silva, Valdeci Elias dos Santos Júnior, Paulo Correia de Melo Júnior, Manuela Arnaud, Maria Goretti Lima, Miguel Angel Pelagio Flores, Thayza Christina Montenegro Stamford, José Ricardo Dias Pereira, Andréa Gadelha Ribeiro Targino, André Galembeck, Aronita Rosenblatt

“…Fragments of deciduous enamel were treated with dentifrice slurries, containing bacterial suspension and PBS-treated saliva. The quantification of the microorganisms that adhered to the enamel was determined after 24 hours of incubation, and media pH readings were performed after 2 hours and 24 hours. …”
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Systemic Resolvin E1 (RvE1) Treatment Does Not Ameliorate the Severity of Collagen-Induced Arthritis (CIA) in Mice: A Randomized, Prospective, and Controlled Proof of Concept Study by Rafael Scaf de Molon, Rogier M. Thurlings, Birgitte Walgreen, Monique M. Helsen, Peter M. van der Kraan, Joni Augusto Cirelli, Marije I. Koenders

“…Thus, this study is aimed at determining whether systemic RvE1 treatment reduces arthritis onset and severity in murine collagen-induced arthritis (CIA) and spontaneous cytokine production by human rheumatoid arthritis (RA) synovial explants. 10-week-old DBA1/J male mice were subjected to CIA and treated systemically with 0.1 μg RvE1, 1 μg RvE1, 5 mg/kg anti-TNF (positive control group), PBS (negative control group), or with a combination of 1 μg of RvE1 plus 5 mg/kg anti-TNF using prophylactic or therapeutic strategies. …”
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Combined Transplantation of Adipose Tissue-Derived Stem Cells and Endothelial Progenitor Cells Improve Diabetic Erectile Dysfunction in a Rat Model by Qiyun Yang, Wanmei Chen, Chi Zhang, Yun Xie, Yong Gao, Cuncan Deng, Xiangzhou Sun, Guihua Liu, Chunhua Deng

“…The normal group and the DED group were received intracavernous injection with phosphate-buffered saline (PBS). And the other groups were received intracavernous injection with ADSCs (1×106), EPCs (1×106), and ADSCs/EPCs (0.5×106/0.5×106), respectively. …”
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Potential Effect of Exosomes Derived from Cancer Stem Cells and MSCs on Progression of DEN-Induced HCC in Rats by Faisal A. Alzahrani, Mohammed A. El-Magd, Ahmed Abdelfattah-Hassan, Ayman A. Saleh, Islam M. Saadeldin, Eman S. El-Shetry, Abdelnaser A. Badawy, Saleh Alkarim

“…CSCs-exosomes induced a significant increase in liver relative weight and serum levels of cancer markers (AFP and GGT) and liver enzymes (ALT, AST, and ALP), intensive immunostaining for the HCC marker GST-P, and an increased number and area of tumor nodules as compared to HCC rats injected by PBS. CSCs-exosomes also decreased apoptosis (marked by downregulation of Bax and p53 and upregulation of Bcl2, and increased immunostaining of PCNA), increased angiogenetic activity (revealed by upregulation of VEGF), enhanced metastasis and invasiveness (indicated by upregulation of P13K and ERK proteins and their downstream target MMP9 and downregulation of TIMP1), and induced epithelial mesenchymal transition (marked by increased serum and hepatic level of TGFβ1 mRNA and protein). …”
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Edaravone dexborneol alleviates pericyte-mediated fibrosis depositing extracellular matrix through TGF-β1/IL-11 in cerebral small vessel disease by Qingrui Duan, Zhiyang Liu, Yuxuan Xing, Haifeng Huang, Lin Zhu, Jiaxuan Liu, Peikun He, Guixian Ma, Yuhu Zhang, Kun Nie, Yuyuan Gao, Lijuan Wang

“…Methods We constructed the CCH mouse models that were subjected to either PBS or EDB at different concentrations. Measures of cognitive function, neuronal damage, white matter lesion (WML), the fibrous profiles of pericytes and ECM protein were investigated to assess the effect of EDB. …”
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Acute Myocardial Infarction Reduces Respiration in Rat Cardiac Fibers, despite Adipose Tissue Mesenchymal Stromal Cell Transplant by Camila I. Irion, Eduarda L. Martins, Michelle L. A. Christie, Cherley B. V. de Andrade, Alan C. N. de Moraes, Raphaela P. Ferreira, Cibele F. Pimentel, Grazielle D. Suhett, Antonio Carlos C. de Carvalho, Rafael S. Lindoso, Adalberto Vieyra, Antonio Galina, Regina C. S. Goldenberg

“…Female rats were subjected to AMI by permanent left anterior descending coronary (LAD) ligation and were then treated with AD-MSCs or PBS in the border zone (BZ). Cardiac fibers were analyzed 24 hours (necrotic phase) and 8 days (fibrotic phase) after AMI for mitochondrial respiration, citrate synthase (CS) activity, F0F1-ATPase activity, and transmission electron microscopy (TEM). …”
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Carboxylated Graphene: An Innovative Approach to Enhanced IgA-SARS-CoV-2 Electrochemical Biosensing by Luciana de Souza Freire, Ariamna María Dip Gandarilla, Yonny Romaguera Barcelay, Camila Macena Ruzo, Barbara Batista Salgado, Ana P. M. Tavares, Francisco Xavier Nobre, Julio Nino de Souza Neto, Spartaco Astolfi-Filho, Ștefan Țălu, Pritesh Lalwani, Niranjan Patra, Walter Ricardo Brito

“…The developed biosensor efficiently detects IgA-SARS-CoV-2 across a dilution range of 1:1000 to 1:200 <i>v</i>/<i>v</i> in a phosphate-buffered saline (PBS) solution, with a limit of detection calculated at 1:1601 <i>v</i>/<i>v</i>. …”
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[^99mTc]Technetium and Rhenium Dithiocarbazate Complexes: Chemical Synthesis and Biological Assessment by André Gustavo de Araujo Fernandes, Alyne Eloise Lafratta, Carolina Portela Luz, Debora Levy, Daniele de Paula Faria, Carlos Alberto Buchpiguel, Ulrich Abram, Victor Marcelo Deflon, Fabio Luiz Navarro Marques

“…The radioactive complex is hydrophobic (LogP = 1.03), stable for 6 h in PBS and L-histidine solution; stable for 1 h in plasma, but unstable in the presence of L-cysteine. …”
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Assessment tuberculin skin test specificity in water buffalo using five different antigens by Alessandra Martucciello, Francesco Napolitano, Piera Mazzone, Francesco Grandoni, Maria Beatrice Boniotti, Monica Cagiola, Gabriele Di Vuolo, Felice Gerardo Giglio, Francesco Grippa, Giorgio Galiero, Federica Signorelli, Esterina De Carlo

“…Five intradermal injection sites on the animal’s shoulder (three on the right and two on the left side) were used on each animal, with a volume of 0.1 mL for all antigens (ESAT-6 and CFP-10 at 20 μg/mL, ESP at 100 μg/mL, PPDA and PPDB at 100 μg/mL), and PBS as negative control. Skin reactions were measured using calipers. …”
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Efficacy of bivalent vaccine against black body syndrome (BBS) of barramundi Lates calcalifer B. by Anis Nugrahawati, Sri Nuryati, Sukenda Sukenda, Rahman Rahman, Margie Brite, Tiya Widi Aditya

“…Barramundi, 5‒6 cm in length, was intraperitoneally injected with bivalent vaccine and phosphate buffer saline (PBS). After a 21-day vaccination, fish was injected with pathogenic bacteria P. stutzeri, V. harveyi, the combination of P. stutzeri and V. harveyi with a dose of bacteria 107 CFU/fish. …”
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Experimental use of mycobacterial antigens in tuberculin skin test on water buffalo by Alessandra Martucciello, Federica Signorelli, Piera Mazzone, Francesco Grandoni, Giovanna Cappelli, Lucia Boccia, Giuseppe Parente, Monica Cagiola, Maria Beatrice Boniotti, Esterina De Carlo, Francesco Napolitano, Giorgio Galiero

“…PPDB and PPDA were always included in the TST, along with PBS as negative control. Skin reactions were measured with calipers before the test and every 24 h for 4 days. …”
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Uncovering the Mechanisms of BaBaoWuDanYaoMo Against Influenza A Virus and Virus-Induced Inflammation Based on Network Pharmacology and Pharmacological Evaluation by Lei B, Su Y, Chen R, Chen Z, Liu B, Chen Y, Zhou M, Wang X, Ma Q

“…The normal group was orally administrated PBS for 5 days. Lung indexes, histological changes and cytokines were determined on the 6th day.Results: BB could effectively inhibit A/Puerto Rico/8/1934 (H1N1), A/GZ/GIRD07/09 (H1N1), A/HK/Y280/97 (H9N2) and A/Aichi/68 (H3N2) with IC50 values of 116.5 ± 2.2, 59.86 ± 8.33, 102.87 ± 6.66 and 66.43 ± 6.785 μg/mL, respectively. …”
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