Genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture by D. Grauda, K. Žagata, G. Lanka, V. Strazdina, V. Fetere, N. Lisina, N. Krasnevska, O. Fokina, A. Mikelsone, R. Ornicans, I. Belogrudova, I. Rashal

“…Flow cytometry was used to test ploidy of 3×103 cells of each green plantregenerant, and universal retrotransposon based iPBS (inter primer binding sites) method were used to establish genetic diversity of plants-regenerants. …”
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SPR Biosensor Based on Bilayer MoS₂ for SARS-CoV-2 Sensing by Talia Tene, Stefano Bellucci, Cristian Vacacela Gomez

“…The optimized configuration, consisting of a 45 nm silver layer, a 13 nm silicon nitride layer, 2 MoS₂ layers, and a 5 nm ssDNA layer, demonstrated superior performance for detecting SARS-CoV-2 in PBS solution. The biosensor exhibited high sensitivity at low viral concentrations, achieving a sensitivity of 375.01°/RIU, a detection accuracy of 0.002, and a quality factor of 38.34 at 1.0 mM SARS-CoV-2 concentration. …”
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IL-33 Aggravates DSS-Induced Acute Colitis in Mouse Colon Lamina Propria by Enhancing Th2 Cell Responses by Junfeng Zhu, Fangli Yang, Lixuan Sang, Jingbo Zhai, Xiaoqing Zhang, Dan Yue, Shengjun Li, Yan Li, Changlong Lu, Xun Sun

“…We found that DSS-induced acute colitis was aggravated by rIL-33 treatment. rIL-33-treated DSS mice showed markedly reduced levels of interferon- (IFN-)γ and IL-17A in their colon lamina propria lymphocytes (LPL), but the levels of Th2 cytokines, such as IL-5 and IL-13, in these cells were significantly increased, compared to DSS mice treated with PBS. Our results suggested that IL-33 stimulated CD4+T cells and caused the cell to adopt a Th2-type response but at the same time suppressed Th17 and Th1 cell responses. …”
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Machine Learning-Based Normal White Blood Cell Multi-Classification Optimization by Taeyeon Gil, Sukjun Lee, Onseok Lee

“…The proposed system in this study is expected to enhance the efficiency of the existing PBS tests.…”
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Rectal application of a highly osmolar personal lubricant in a macaque model induces acute cytotoxicity but does not increase risk of SHIV infection. by Sundaram A Vishwanathan, Monica R Morris, Richard J Wolitski, Wei Luo, Charles E Rose, Dianna M Blau, Theodros Tsegaye, Sherif R Zaki, David A Garber, Leecresia T Jenkins, Tara C Henning, Dorothy L Patton, R Michael Hendry, Janet M McNicholl, Ellen N Kersh

“…<h4>Results</h4>Lubricant increased pro-inflammatory cytokines and tissue sloughing while control buffer (phosphate buffered saline; PBS) did not. However, the estimated AID50 (50% animal infectious dose) was not different in lubricant- and control buffer-treated macaques (p = 0.4467; logistic regression models).…”
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Duration of feed application of mixed powders of Phyllanthus niruri and Allium sativum for the prevention of Aeromonas hydrophila infection in catfish Clarias sp. by Dinamella Wahjuningrum, Iis Widiani, Sri Nuryati

“…This research tested on three different of durations of feed application of P. niruri and A. sativum, namely A (powder of P. niruri and A. sativum feed application during 21 days and infected with A. hydrophila 0,1 mL IP), B (powder of P. niruri and A. sativum feed application during 14 days and injected IP with A. hydrophila 0,1 mL), C (powder of P. niruri and A. sativum feed application during seven days and injected IP with A. hydrophila 0,1 mL), and control, namely K- (without P. niruri and A. sativum feed application and injected IP with PBS 0,1 mL) and K+ (without P. niruri and A. sativum powder application and injected IP with A. hydrophila 0,1 mL). …”
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Bound lipase: an important form of lipase in rice bran (Oryza sativa) by Chengwei Yu, Bin Peng, Ting Luo, Zeyuan Deng

“…Rice bran residue possessed a steady lipase activity ((26.68 ± 3.69)%) after its endogenous lipase was extracted continuously by phosphate buffer solution (PBS) for 24 h. Therefore, the aim of this research was to explore whether there exist any bound lipases in rice bran (Oryza sativa). …”
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Assembled Nanostructured Architectures Studied By Grazing Incidence X-Ray Scattering by Davide Altamura, Teresa Sibillano, Dritan Siliqi, Liberato De Caro, Cinzia Giannini

“…Further examples of grazing incidence studies as performed by the authors with a table‐top set‐up are reported: a GISAXS study of 3D iron oxide nanocrystal superlattices, showing the importance of modelling in order to obtain structural information from data; a combined small/wide angle scattering (GISAXS/GIWAXS) study of 3D PbS nanocrystal superlattices; and a GIWAXS study of P3HT nanofibres, showing how the ordering at the molecular and atomic length scales can be obtained by exploring different angular ranges in the same grazing incidence geometry. …”
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Upregulation of TCPTP in Macrophages Is Involved in IL-35 Mediated Attenuation of Experimental Colitis by Baoren Zhang, Chenglu Sun, Yanglin Zhu, Hong Qin, Dejun Kong, Jingyi Zhang, Bo Shao, Xiang Li, Shaohua Ren, Hongda Wang, Jingpeng Hao, Hao Wang

“…Mice were treated with Pichia/IL-35, empty plasmid-transformed Pichia (Pichia/0), or PBS by gavage, respectively. The expression of TCPTP in macrophages (RAW264.7, BMDMs) and intestinal tissues after IL-35 treatment was detected. …”
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Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin Prevent the Expression of Proinflammatory Chemokines and Cytokines in Pigs by R. Frandoloso, S. Martínez-Martínez, E. F. Rodríguez-Ferri, S. Yubero, D. Rodríguez-Lázaro, M. Hernández, C. B. Gutiérrez-Martín

“…Two vaccines containing native proteins with affinity to porcine transferrin (NPAPTim and NPAPTit) were tested, along with two control groups: one inoculated with PBS instead of antigen (challenge group (CHG)), and another one nonimmunized and noninfected (blank group). …”
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Efficient Self-Assembly of mPEG End-Capped Porous Silica as a Redox-Sensitive Nanocarrier for Controlled Doxorubicin Delivery by Anh Khoa Nguyen, Thi Hiep Nguyen, Bui Quoc Bao, Long Giang Bach, Dai Hai Nguyen

“…The release of DOX from DOX/PNS-SS-ADA@CD-mPEG nanoparticles was controlled and prolonged up to 120 h in PBS medium (pH 7.4), compared to less than 40 h under reducing condition of 5 mM DTT. …”
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Tetrandrine Inhibits Titanium Particle-Induced Inflammatory Osteolysis through the Nuclear Factor-κB Pathway by Zige Liu, Yan Li, Fengying Guo, Chen Zhang, Guorui Song, Jiahao Yang, Desheng Chen

“…We established a Ti particle-induced inflammatory osteolysis mouse model and administered Tet or an equal volume of phosphate-buffered saline (PBS). Two weeks later, specimens were collected. …”
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Efficacy of human umbilical cord mesenchymal stem cell in the treatment of neuromyelitis optica spectrum disorders: an animal study by Chunran Xue, Haojun Yu, Xuzhong Pei, Xiaoying Yao, Jie Ding, Xiying Wang, Yi Chen, Yangtai Guan

“…Methods 106 hUC-MSCs prepared in 200 μl PBS were intravenously administered to a systemic NMOSD model on day 10 and day 14 after immunization. …”
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Efficacy of canal disinfectants Temoporfin, carbon nanoparticles, and Er: YAG laser on martens hardness, smear layer removal, and bond strength of glass fiber posts to canal dentin by Abdulaziz Alsahhaf

“…Aim: To investigate the effectiveness of unconventional post space disinfection techniques, specifically Er:YAG laser, Temoporfin, and Carbon Nanoparticles (CNPs), in improving Martens hardness (MH), eliminating the smear layer (SL), and enhancing the push-out bond strength (PBS) of glass fiber posts in canal dentin. Methods: An in vitro study was conducted utilizing 80 single-rooted extracted human teeth, which were decoronated and subjected to standardized root canal preparation. …”
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Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse by Lisi Deng, Guangze Liu, Xin Wu, Yaping Wang, Minghua Tong, Bing Liu, Kuidong Wang, Yuting Peng, Xiangping Kong

“…The survival rate of transplantation group significantly increased compared to PBS group. Furthermore, the transplanted cells were well integrated into injured livers and produced albumin, cytokeratin-18. …”
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Evaluation of Sericin/Polyvinyl Alcohol Mixtures for Developing Porous and Stable Structures by Maria C. Arango, Leander Vásquez Vásquez, Akemy Carolina Homma Parra, Santiago Rueda-Mira, Natalia Jaramillo-Quiceno, Josep Pasqual Cerisuelo, Amparo Cháfer, Catalina Álvarez-López

“…This study explored the effects of different SS/polyvinyl alcohol (PVA) ratios on porous scaffolds fabricated via freeze-drying, focusing on the mechanical stability, water absorption, and protein release in phosphate-buffered saline (PBS). The scaffold morphology revealed reduced porosity with higher SS content, while increased PVA content led to material folding and layering. …”
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Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice by Yangwenjie Wang, Yang Xiang, Ruiqi Wang, Xiangning Li, Jianxiong Wang, Siwang Yu, Ying Zhang

“…Mice were intraperitoneally injected with SFN (HIIT+SFN group) or PBS (HIIT and control mice) 4 times in 3 days prior to the exhaustive treadmill test. …”
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Efficiency of the Stool-PCR Test Targeting NADH Dehydrogenase (Nad) Subunits for Detection of Opisthorchis viverrini Eggs by Wansika Phadungsil, Supaporn Pumpa, Kridsada Sirisabhabhorn, Amornrat Geadkaew-Krenc, Rudi Grams, Mathirut Mungthin, Toon Ruang-Areerate, Poom Adisakwattana, Nipawan Labbunruang, Pongsakorn Martviset

“…A total of 166 stool samples were investigated microscopically by the PBS-ethyl acetate concentration technique (PECT); 75 samples were O. viverrini positive with 28 samples that were positive with single parasite (hookworm, A. lumbricoides, S. stercoralis, Taenia spp., and T. trichiura), 11 samples were with mixed infection, and 52 samples were without parasite detection. …”
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Candidate genes for domestication and resistance to cold climate according to whole genome sequencing data of Russian cattle and sheep breeds by N. S. Yudin, D. M. Larkin

“…We used the HapFLK, DCMS, FST and PBS methods to identify DNA regions with signatures of selection. …”
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Hydrogels of Poly(2-hydroxyethyl methacrylate) and Poly(N,N-dimethylacrylamide) Interpenetrating Polymer Networks as Dermal Delivery Systems for Dexamethasone by Marin Simeonov, Bistra Kostova, Rositsa Mihaylova, Elena Vassileva

“…Drug release profiles were studied in Franz diffusion cells in PBS media. Finally, the cytotoxicity of the PHEMA/PDMAM-based IPNs was studied against T-cell lymphoma cells (HUT-78) and a normal murine fibroblast cell line (CCL-1). …”
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