Potential Effect of Exosomes Derived from Cancer Stem Cells and MSCs on Progression of DEN-Induced HCC in Rats by Faisal A. Alzahrani, Mohammed A. El-Magd, Ahmed Abdelfattah-Hassan, Ayman A. Saleh, Islam M. Saadeldin, Eman S. El-Shetry, Abdelnaser A. Badawy, Saleh Alkarim

“…CSCs-exosomes induced a significant increase in liver relative weight and serum levels of cancer markers (AFP and GGT) and liver enzymes (ALT, AST, and ALP), intensive immunostaining for the HCC marker GST-P, and an increased number and area of tumor nodules as compared to HCC rats injected by PBS. CSCs-exosomes also decreased apoptosis (marked by downregulation of Bax and p53 and upregulation of Bcl2, and increased immunostaining of PCNA), increased angiogenetic activity (revealed by upregulation of VEGF), enhanced metastasis and invasiveness (indicated by upregulation of P13K and ERK proteins and their downstream target MMP9 and downregulation of TIMP1), and induced epithelial mesenchymal transition (marked by increased serum and hepatic level of TGFβ1 mRNA and protein). …”
Get full text

Edaravone dexborneol alleviates pericyte-mediated fibrosis depositing extracellular matrix through TGF-β1/IL-11 in cerebral small vessel disease by Qingrui Duan, Zhiyang Liu, Yuxuan Xing, Haifeng Huang, Lin Zhu, Jiaxuan Liu, Peikun He, Guixian Ma, Yuhu Zhang, Kun Nie, Yuyuan Gao, Lijuan Wang

“…Methods We constructed the CCH mouse models that were subjected to either PBS or EDB at different concentrations. Measures of cognitive function, neuronal damage, white matter lesion (WML), the fibrous profiles of pericytes and ECM protein were investigated to assess the effect of EDB. …”
Get full text

铝佐剂Aβ42及其亚单位疫苗可有效诱导大鼠产生特异性抗体

“…将Al3,2肤疫苗及Al3J• _,, 与七聚赖氨酸耦联制成的亚单位疫苗分别接种千SD大鼠， 对照组用等体积PBS加铝佐剂。 用间接ELISA法检测 大鼠血清和脑匀浆上清液中特异性抗体的滴度；用Morris水迷宫测试大鼠的行为学变化；用HE染色观察大鼠 的脑、肝、脾和肾组织学变化；用接种后血清与老年性痴呆患者的脑片作免疫组织化学染色。…”
Get full text

Antibiotic-mediated dysbiosis leads to activation of inflammatory pathways by Jemma J. Taitz, Jemma J. Taitz, Jian Tan, Jian Tan, Duan Ni, Duan Ni, Camille Potier-Villette, Camille Potier-Villette, Georges Grau, Georges Grau, Ralph Nanan, Ralph Nanan, Ralph Nanan, Laurence Macia, Laurence Macia, Laurence Macia

“…While the impact of specific metabolites or taxa on host cells is well-documented, the broader impact of a disrupted microbiota on immune homeostasis is less understood, which is particularly important in the context of the increasing overuse of antibiotics.MethodsFemale C57BL/6 mice were gavaged twice daily for four weeks with Vancomycin, Polymyxin B, or PBS (control). Caecal microbiota composition was assessed via 16S rRNA sequencing and caecal metabolites were quantified with NMR spectroscopy. …”
Get full text

Effect and Mechanism of Running on Mouse Emphysema Model Induced by Cigarette Extract by LI Yongrong, XIE Haibin, LI hong, SUN Jie

“…The control group and the exercise group were intraperitoneally injected with 0.3 mL phosphate buffer saline (PBS) at the 0, 11th and 22th day in the experiment; the emphysema group and the emphysema + exercise group were intraperitoneally injected with 0.3 mL cigarette extract at the 0, 11th and 22th day in the experiment. …”
Get full text

Optimization and Evaluation of Conditions for Orthotopic Nude Mouse Models of Human Liver Tumor Cells by MENG Yu, LIANG Dongli, ZHENG Linlin, ZHOU Yuanyuan, WANG Zhaoxia

“…Different concentrations (8×106, 2.4×107, 7.2×107 cells/mL) and resuspension media (PBS, Matrigel) of human liver tumor cell suspensions HepG2-LUC and Hep3B-LUC were orthotopically inoculated into the liver lobes of 5-week-old female BALB/c nude mice (12 groups, 7 mice each) to construct human liver tumor nude mouse orthotopic cancer models. …”
Get full text

Acute Myocardial Infarction Reduces Respiration in Rat Cardiac Fibers, despite Adipose Tissue Mesenchymal Stromal Cell Transplant by Camila I. Irion, Eduarda L. Martins, Michelle L. A. Christie, Cherley B. V. de Andrade, Alan C. N. de Moraes, Raphaela P. Ferreira, Cibele F. Pimentel, Grazielle D. Suhett, Antonio Carlos C. de Carvalho, Rafael S. Lindoso, Adalberto Vieyra, Antonio Galina, Regina C. S. Goldenberg

“…Female rats were subjected to AMI by permanent left anterior descending coronary (LAD) ligation and were then treated with AD-MSCs or PBS in the border zone (BZ). Cardiac fibers were analyzed 24 hours (necrotic phase) and 8 days (fibrotic phase) after AMI for mitochondrial respiration, citrate synthase (CS) activity, F0F1-ATPase activity, and transmission electron microscopy (TEM). …”
Get full text

Carboxylated Graphene: An Innovative Approach to Enhanced IgA-SARS-CoV-2 Electrochemical Biosensing by Luciana de Souza Freire, Ariamna María Dip Gandarilla, Yonny Romaguera Barcelay, Camila Macena Ruzo, Barbara Batista Salgado, Ana P. M. Tavares, Francisco Xavier Nobre, Julio Nino de Souza Neto, Spartaco Astolfi-Filho, Ștefan Țălu, Pritesh Lalwani, Niranjan Patra, Walter Ricardo Brito

“…The developed biosensor efficiently detects IgA-SARS-CoV-2 across a dilution range of 1:1000 to 1:200 <i>v</i>/<i>v</i> in a phosphate-buffered saline (PBS) solution, with a limit of detection calculated at 1:1601 <i>v</i>/<i>v</i>. …”
Get full text

[^99mTc]Technetium and Rhenium Dithiocarbazate Complexes: Chemical Synthesis and Biological Assessment by André Gustavo de Araujo Fernandes, Alyne Eloise Lafratta, Carolina Portela Luz, Debora Levy, Daniele de Paula Faria, Carlos Alberto Buchpiguel, Ulrich Abram, Victor Marcelo Deflon, Fabio Luiz Navarro Marques

“…The radioactive complex is hydrophobic (LogP = 1.03), stable for 6 h in PBS and L-histidine solution; stable for 1 h in plasma, but unstable in the presence of L-cysteine. …”
Get full text

Assessment tuberculin skin test specificity in water buffalo using five different antigens by Alessandra Martucciello, Francesco Napolitano, Piera Mazzone, Francesco Grandoni, Maria Beatrice Boniotti, Monica Cagiola, Gabriele Di Vuolo, Felice Gerardo Giglio, Francesco Grippa, Giorgio Galiero, Federica Signorelli, Esterina De Carlo

“…Five intradermal injection sites on the animal’s shoulder (three on the right and two on the left side) were used on each animal, with a volume of 0.1 mL for all antigens (ESAT-6 and CFP-10 at 20 μg/mL, ESP at 100 μg/mL, PPDA and PPDB at 100 μg/mL), and PBS as negative control. Skin reactions were measured using calipers. …”
Get full text

人嗅黏膜来源间充质干细胞对实验性自身免疫性脑脊髓炎小鼠的治疗作用与机制 by 肖崇珺, 刘秋莉, 黄牡丹, 陈莉琳, 郑海清

“…造模后第16天（发病高峰期）将已发病小鼠随机分为治疗组和对照组，分别进行经尾静脉OMSC和等体积PBS治疗。造模后第24天经内眦静脉取血，CBA法检测小鼠血浆IL-10、IL-17、IFN-γ、IL-6水平。…”
Get full text

Efficacy of bivalent vaccine against black body syndrome (BBS) of barramundi Lates calcalifer B. by Anis Nugrahawati, Sri Nuryati, Sukenda Sukenda, Rahman Rahman, Margie Brite, Tiya Widi Aditya

“…Barramundi, 5‒6 cm in length, was intraperitoneally injected with bivalent vaccine and phosphate buffer saline (PBS). After a 21-day vaccination, fish was injected with pathogenic bacteria P. stutzeri, V. harveyi, the combination of P. stutzeri and V. harveyi with a dose of bacteria 107 CFU/fish. …”
Get full text

Experimental use of mycobacterial antigens in tuberculin skin test on water buffalo by Alessandra Martucciello, Federica Signorelli, Piera Mazzone, Francesco Grandoni, Giovanna Cappelli, Lucia Boccia, Giuseppe Parente, Monica Cagiola, Maria Beatrice Boniotti, Esterina De Carlo, Francesco Napolitano, Giorgio Galiero

“…PPDB and PPDA were always included in the TST, along with PBS as negative control. Skin reactions were measured with calipers before the test and every 24 h for 4 days. …”
Get full text

Uncovering the Mechanisms of BaBaoWuDanYaoMo Against Influenza A Virus and Virus-Induced Inflammation Based on Network Pharmacology and Pharmacological Evaluation by Lei B, Su Y, Chen R, Chen Z, Liu B, Chen Y, Zhou M, Wang X, Ma Q

“…The normal group was orally administrated PBS for 5 days. Lung indexes, histological changes and cytokines were determined on the 6th day.Results: BB could effectively inhibit A/Puerto Rico/8/1934 (H1N1), A/GZ/GIRD07/09 (H1N1), A/HK/Y280/97 (H9N2) and A/Aichi/68 (H3N2) with IC50 values of 116.5 ± 2.2, 59.86 ± 8.33, 102.87 ± 6.66 and 66.43 ± 6.785 μg/mL, respectively. …”
Get full text

Application of probiotic microcapsules Bacillus cereus BR2 with different doses for the prevention of Aeromonas hydrophila NFC1 infection in catfish Clarias sp. by Nurul Faoziyatunnisa, Munti Yuhana, Dinamella Wahjuningrum, Sri Nuryati, Usamah Afiff

“…Rancangan penelitian yang digunakan terdiri dari lima perlakuan dan tiga ulangan, yaitu (K-) pemberian pakan tanpa suplementasi probiotik dan diinjeksi PBS; (K+) pemberian pakan tanpa suplementasi probiotik dan diinfeksi A. hydrophila NFC1; (P1) pemberian pakan dengan suplementasi mikrokapsul B. cereus BR2 dosis 0,5% (b/b); (P2) pemberian pakan dengan suplementasi mikrokapsul B. cereus BR2 dosis 1%; (P3) pemberian pakan dengan suplementasi mikrokapsul B. cereus BR2 dosis 2%, masing-masing diinfeksi A. hydrophila NFC1. …”
Get full text

Transcriptomic and proteomic analysis of the jejunum revealed the effects and mechanism of protocatechuic acid on alleviating Salmonella typhimurium infection in chickens by Sheng Zhang, Qin Wang, Xiangtian Yao, Jingjing Dong, Guanhuo Li, Yingan Zang, Shouqun Jiang, Yibing Wang

“…On 14 and 16 d, birds in Sal and PA were orally challenged with 109 CFU S. typhimurium, while birds in Ctr received an equal amount of PBS. The results showed that protocatechuic acid improved growth performance and jejunal structure in Salmonella-infected chickens (P < 0.05). …”
Get full text

小鼠软脑膜淋巴管内皮细胞的发育特点及其对行为功能的影响 by 谭立人, 肖颂华, 雷鸣

“…将26只2周龄C57BL小鼠随机进行如下分组：PBS注射组 （<italic>n</italic>=10）、anti-Lyve-1中和抗体注射组 （<italic>n</italic>=9）、SAR131675小分子抑制剂注射组 （<italic>n</italic>=7），3组注射剂量均为2 μL。…”
Get full text

宏基因组联合UPLC-Q-TOF-MS/MS探讨地塞米松对大鼠肺炎的作用机制 by 李四菊, 张倩, 冷云, 潘碧妍, 谢智勇, 陈红英

“…然后Dex组给予2 mg/kg的Dex灌胃治疗12 d，余下两组均连续灌胃等量的无菌PBS缓冲液12 d。第19 d时收集大鼠的肺组织、血浆、粪便和肠道内容物。苏木精-伊红（H&amp;E）染色和Bio-plex悬浮芯片系统评价Dex的药效。…”
Get full text

Comparative evaluation of ACetic - MEthanol high salt dissociation approach for single-cell transcriptomics of frozen human tissues by Marina Utkina, Anastasia Shcherbakova, Ruslan Deviatiiarov, Ruslan Deviatiiarov, Ruslan Deviatiiarov, Alina Ryabova, Marina Loguinova, Valentin Trofimov, Anna Kuznetsova, Mikhail Petropavlovskiy, Rustam Salimkhanov, Denis Maksimov, Eugene Albert, Alexandra Golubeva, Walaa Asaad, Lilia Urusova, Ekaterina Bondarenko, Anastasia Lapshina, Alexandra Shutova, Dmitry Beltsevich, Oleg Gusev, Oleg Gusev, Oleg Gusev, Oleg Gusev, Larisa Dzeranova, Galina Melnichenko, Ildar Minniakhmetov, Ivan Dedov, Natalya Mokrysheva, Sergey Popov

“…We incorporated a high-salt washing buffer instead of the standard PBS to stabilize RNA and prevent RNases reactivation during rehydration. …”
Get full text

Therapeutic efficacy of LysGH15 against necrotising pneumonia caused by Staphylococcus aureus in a rabbit model by Bowei Zhang, Liran Song, Yongran Wang, Meimei Zhang, Chong Chen, Hui Ning, Li Wang, Cao Qiu, Xinwu Wang, Changjiang Sun, Xin Feng, Wenyu Han, Wenyu Han, Bin Wang, Yalu Ji, Jingmin Gu, Jingmin Gu

“…At 72 h postinfection, the number of bacteria in the lung tissues of the rabbits treated with LysGH15 was 7 × 104 CFU/g, which was significantly lower than that in the lung tissues of rabbits treated with PBS (7.76 × 106 CFU/g) or linezolid (6.38 × 105 CFU/g). …”
Get full text