Identification of Type VI Collagen Synthesizing Cells in Human Diabetic Glomerulosclerosis Using Renal Biopsy Sections by Mohammed Shawkat Razzaque, Takehiko Koji, Takashi Harada, Takashi Taguchi

“…Type VI collagen‐specific oligonucleotide probes and monoclonal antibody were used to assess the relative expression of mRNA for \alpha1 (VI) chain and its translated protein in paraffin‐embedded renal biopsy sections of DN. …”
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The Use of Antisense-Mediated Inhibition to Delineate The Role of Inflammatory Agents in The Pathophysiology of Spinal Cord Injury by Damien D. Pearse, Francisco C. Pereira, Katina Chatzipanteli, Francisco W. Dalton Dietrich, Mary Bartlett Bunge

“…The secretion of these factors may be primarily responsible for secondary damage to surrounding uninjured tissue that potentiates the initial injury[3]. Antisense oligonucleotides (ASOs) are designed to hybridize to specific regions of specific mRNAs. …”
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PWS/AS MS-MLPA Confirms Maternal Origin of 15q11.2 Microduplication by Angelika J. Dawson, Janice Cox, Karine Hovanes, Elizabeth Spriggs

“…In the present study, a four-year-old nondysmorphic female patient with developmental delay was found to have a de novo ~5 Mb duplication within 15q11.2 by oligonucleotide genomic array. In order to determine the significance of this microduplication to the clinical phenotype, the parent-of-origin needed to be identified. …”
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16S rRNA gene sequencing of non-lactic acid bacteria cultures from lait caillé in Burkina Faso by Geoffroy Romaric Bayili, Clarisse S. Compaoré, Bréhima Diawara, Lene Jespersen, Hagretou Sawadogo-Lingani

“…Pure colonies were selected, then the penta-GTG oligonucleotide (GTG)5-based rep-PCR fingerprinting followed by cluster analysis and sequencing of the 16S rRNA gene was performed to identify the isolates. …”
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Role of HLA alleles polymorphism in systemic lupus erythematosus: A prospective study from North India by Ranjan S. Rana, Bitan Naik, Mahima Yadav, Usha Singh, Anup Singh, Shailja Singh

“…HLA typing was done by polymerase chain reaction (PCR)-sequence-specific oligonucleotide (SSO) method (SSO probe). Results: DRβ1*0301 was significantly increased in SLE patients when compared to controls and had the highest odds ratio. …”
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Genetic Analysis Reveals A Missense Mutation In Reactivation Gene-2 Causing Severe Combined Immunodeficiency Disease In A Pakistani Family by Shahida Awais, Lubna Siddique, Nida Shafi, Sobia Humerah, Summyah Niazi, Wafa Omer

“…Exons specific polymerase chain reaction (PCR) was performed using oligonucleotide primers and subsequently DNA Sanger sequenced which resulted into an missense variant in the gene RAG2. …”
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Genetic Dissection of Sympatric Populations of Brown Planthopper, Nilaparvata lugens (Stål), Using DALP-PCR Molecular Markers by M. A. Latif, M. Y. Rafii, M. S. Mazid, M. E. Ali, F. Ahmed, M. Y. Omar, S. G. Tan

“…N. bakeri was used as an out group. Three oligonucleotide primer pairs, DALP231/DALPR′5, DALP234/DALPR′5, and DALP235/DALPR′5 were applied in this study. …”
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Hybridization State Detection of DNA-Functionalized Gold Nanoparticles Using Hyperspectral Imaging by Richard C. Murdock, Omar A. Khan, Thomas J. Lamkin, Saber M. Hussain, Nancy Kelley-Loughnane

“…In this study, 4 nm and 13 nm gold nanoparticles (Au NPs) were synthesized, functionalized with complimentary oligonucleotides, and hybridized to form large networks of NPs. …”
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Transient degradation of NF-κB proteins in macrophages after interaction with mast cell granules by Noriko Ito, Yuai Li, Tsuneo Suzuki, Daniel J. Stechschulte, Kottarappat N. Dileepan

“…These data indicate that exposure of J774 cells to MCG leads to generation of altered nuclear p52, p65 and c-Rel, which retain intact N-terminal peptides, specific oligonucleotide binding and transactivating activity. …”
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Azurin a potent anticancer and antimicrobial agent isolated from a novel Pseudomonas aeruginosa strain by Nourhan A. Zaghloul, Mona K. Gouda, Yasser Elbahloul, Nancy M. El Halfawy

“…The presence of the azurin gene was confirmed in the genomic DNA using specific oligonucleotide primers, and azurin expression was also detected in the synthesized cDNA, which revealed that the azurin expression is active. …”
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Isolation, identification, and quantification of Prevotella intermedia and Prevotella nigrescens in subgingival plaque samples of periodontally healthy and chronic periodontitis pa... by Vijayalakshmi Sharadindu Kotrashetti, Kishore Gajanan Bhat, Vijay Mahadev Kumbar, Sachita Shrikant Naik

“…The slides were stained using a specific oligonucleotide probe for PI and PN and then subjected to streptavidin-biotinylated horseradish peroxidase complex. …”
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Transcriptome-Wide Analysis Reveals the Role of PPARγ Controlling the Lipid Metabolism in Goat Mammary Epithelial Cells by Hengbo Shi, Wangsheng Zhao, Changhui Zhang, Khuram Shahzad, Jun Luo, Juan J. Loor

“…To explore the large-scale effect of peroxisome proliferator-activated receptor γ (PPARG) in goat mammary epithelial cells (GMEC), an oligonucleotide microarray platform was used for transcriptome profiling in cells overexpressing PPARG and incubated with or without rosiglitazone (ROSI, a PPARγ agonist). …”
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Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva by Paweł Wityk, Agata Terebieniec, Robert Nowak, Jacek Łubiński, Martyna Mroczyńska-Szeląg, Tomasz Wityk, Dorota Kostrzewa-Nowak

“…The biosensor is based on electrochemical principles, employing oligonucleotide probes immobilized on a hydrophobic-coated electrode, which prevents air bubbles and salt crystal formation. …”
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Combining Microarray Technology and Molecular Epidemiology to Identify Genes Associated with Invasive Group B Streptococcus by Lixin Zhang, Usha Reddi, Usha Srinivasan, Sheng Li, Stephanie M. Borchardt, Parvathy Pillai, Puja Mehta, Anne N. Styka, Joan DeBusscher, Carl F. Marrs, Betsy Foxman

“…Comparative hybridization using an oligonucleotide microarray, combined with epidemiologic screening using the LOS microarray platform, enabled rapid identification of bacterial genes potentially associated with pathogenicity.…”
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Inhibiting C-Reactive Protein for the Treatment of Cardiovascular Disease: Promising Evidence from Rodent Models by Alexander J. Szalai, Mark A. McCrory, Dongqi Xing, Fadi G. Hage, Andrew Miller, Suzanne Oparil, Yiu-Fai Chen, Michelle Mazzone, Richard Early, Scott P. Henry, Thomas A. Zanardi, Mark J. Graham, Rosanne M. Crooke

“…Blood CRP is synthesized primarily in the liver and the liver is an organ where antisense oligonucleotide (ASO) drugs accumulate. Taking advantage of this we evaluated the efficacy of CRP specific ASOs in rodents with experimentally induced cardiovascular damage. …”
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The Study of Viral RNA Diversity in Bird Samples Using De Novo Designed Multiplex Genus-Specific Primer Panels by Andrey A. Ayginin, Ekaterina V. Pimkina, Alina D. Matsvay, Anna S. Speranskaya, Marina V. Safonova, Ekaterina A. Blinova, Ilya V. Artyushin, Vladimir G. Dedkov, German A. Shipulin, Kamil Khafizov

“…However, unlike the metagenomic studies using 16S rRNA for the detection of bacteria, it is impossible to create universal oligonucleotides to target all known and novel viruses, owing to their genomic diversity and variability. …”
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Development of real-time RT-PCR for N2 subtype avian influenza RNA-virus detection by P. B. Akshalova, A. V. Andriyasov, L. O. Scherbakova, S. N. Kolosov, N. G. Zinyakov, I. A. Chvala, D. B. Andreychuk

“…Therefore, a method for rapid detection of the said infectious agent is urgently required. Data on oligonucleotide primer selection and reverse transcription real-time polymerase chain reaction condition optimization for N2 AI virus detection are presented in the paper. …”
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Flanking monomer repeats define lower context complexity of sites containing single nucleotide polymorphisms in the human genome by N. S. Safronova, M. P. Ponomarenko, I. I. Abnizova, G. V. Orlova, I. V. Chadaeva, Y. L. Orlov

“…Such oligonucleotides are related to nucleotide polytracks. …”
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Long noncoding RNA GDIL acts as a scaffold for CHAC1 and XRN2 to promote platinum resistance of colorectal cancer through inhibition of glutathione degradation by Xuan Deng, Lu Chang, Lingyu Tang, Haoqin Jiang, Xiao Xu, Xinju Zhang, Jian Chen, Liu Dong, Qianqian Xu, Ruoshui Cao, Jianbin Xiang, Ming Guan

“…Suppression of GDIL with selective antisense oligonucleotide, restored drug sensitivity in platinum resistant cell lines and delayed drug resistance in cell line- and patient-derived xenografts. …”
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Monoallelic variants resulting in substitutions of MAB21L1 Arg51 Cause Aniridia and microphthalmia. by Hildegard Nikki Hall, Hemant Bengani, Robert B Hufnagel, Giuseppe Damante, Morad Ansari, Joseph A Marsh, Graeme R Grimes, Alex von Kriegsheim, David Moore, Lisa McKie, Jamalia Rahmat, Catia Mio, Moira Blyth, Wee Teik Keng, Lily Islam, Meriel McEntargart, Marcel M Mannens, Veronica Van Heyningen, Joe Rainger, Brian P Brooks, David R FitzPatrick

“…The predicted nucleotidyltransferase function of MAB21L1 could not be demonstrated using purified protein with a variety of nucleotide substrates and oligonucleotide activators. Induced expression of GFP-tagged wildtype and mutant MAB21L1 in human cells caused only modest transcriptional changes. …”
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