A 7-Amino Acid Peptide Mimic from Hepatitis C Virus Hypervariable Region 1 Inhibits Mouse Lung Th9 Cell Differentiation by Blocking CD81 Signaling during Allergic Lung Inflammation by Wanzhou Zhao, Conghao Tan, Xi Yu, Ruihe Yu, Qibing Mei, Yun Cheng

“…However, it is unknown whether 7P regulates Th9 cell differentiation during ovalbumin- (OVA-) induced allergic lung inflammation. To address this, we studied wild-type mice treated with 7P and a control peptide in an in vivo mouse model of OVA-induced allergic inflammation and an in vitro cell model of Th9 differentiation, using flow cytometry, cytokine assays, and quantitative PCR. …”
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Interferon-γ production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particles by Takeharu Watanabe, Masayuki Hayashi, Masaaki Arai, Sho Matsushita, Hiroshi Handa, Masaaki Kawano

“…In this study, administration of chicken ovalbumin (OVA) incorporated within SV40 VLPs induced interferon (IFN)-γ production in response to the OVA CTL epitope. …”
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Differential Regulation of DC Function, Adaptive Immunity, and MyD88 Dependence by Two Squalene Emulsion-Based Vaccine Adjuvants by Jayachandra Reddy Nakkala, Yibo Li, Labone Akter, Xinliang Kang, Xinyuan Chen

“…AddaVax and AddaS03 similarly enhanced OVA-induced IgG and subtype IgG1 antibody responses as well as influenza vaccine-induced hemagglutination inhibition antibody titers, while AddaS03 more potently enhanced OVA-specific IgG2c antibody responses. …”
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白三烯受休拈抗剂孟鲁斯特对小鼠气道炎症的抑制作用 by 李雯

“…【方法｝致敏的BALB/c小鼠待续吸入10叭L雾化卯白蛋白(OVA)30 111in,OVA吸入前2d开始连续3cl 或5J尾静脉给予MK或生理盐水(saline),OVA吸入后24h及72h取材，瑞氏染色观察支气管肺泡泄洗液 (bronchoalveolar lavage fluid, BALF)中炎症细胞的渗出情汜ELISA方法检测血清、BALF和肺组织中有关细胞因子水平；HE、刚果红染色观察肺组织炎症细胞的渗出；免疫组化染色观察血管内皮细胞黏附分子-l(VCAM-1)和 嗜酸性粒细胞趋化因子(eot,ixin)的表达，原位杂交研究IL-5mRNA的变化，［绪果】给予MK5 d减少BALF嗜 酸性粒细胞渗出达90%以上，嗜酸性粒细胞减少随MK剂量(10、25、62.5mg/kg)的增加呈显效关系。…”
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基于16S rDNA基因测序探讨咳嗽变异性哮喘与肠道菌群的关系 by 沈灵, 曾琳琳, 骆巧媚, 范依霖, 贺倩, 段小云

“…目的基于“肺与大肠相表里”中医理论和16S核糖体DNA（16S rDNA）基因测序技术，探讨卵清蛋白（OVA）诱导的咳嗽变异性哮喘（CVA）对小鼠肠道菌群的影响。方法20只BALB/c雌性小鼠随机分为模型组和对照组，以OVA致敏并激发制备CVA小鼠模型。…”
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Modulation of Th1/Th2 Immune Responses by Killed Propionibacterium acnes and Its Soluble Polysaccharide Fraction in a Type I Hypersensitivity Murine Model: Induction of Different A... by Carla Cristina Squaiella-Baptistão, Daniela Teixeira, Juliana Sekeres Mussalem, Mayari Eika Ishimura, Ieda Maria Longo-Maugéri

“…We previously demonstrated that heat-killed P. acnes or its soluble polysaccharide (PS), extracted from the bacterium cell wall, suppressed or potentiated the Th2 response to ovalbumin (OVA) in an immediate hypersensitivity model, depending on the treatment protocol. …”
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T-bet基因转染对哮喘小鼠脾脏Th1/Th2平衡的影响

“…【目的】 观察气道内T-bet基因转染对哮喘小鼠脾脏淋巴细胞Th1/Th2平衡65380;T-bet及GATA-3 mRNA表达的影响65377; 【方法】 将C57BL/6小鼠32只随机分为4组,每组8只,分别为正常对照组(A组)65380;哮喘模型组(B组)65380;空质粒干预组(C组) 和T-bet质粒干预组(D组)65377;卵白蛋白(OVA) 抗原溶液腹腔注射致敏,滴鼻造模65377;空质粒组和T-bet质粒干预组OVA激发24 h前,分别经鼻滴入50 μg空质粒65380;重组T-bet质粒,对照组用生理盐水代替OVA65377;最后一次滴鼻激发48 h后处死小鼠,流式细胞仪检测各组实验小鼠的脾脏淋巴细胞的Th1/Th2, RT-PCR检测小鼠脾脏淋巴细胞的T-bet及GATA-3mRNA表达65377; 【结果】 哮喘小鼠气道转染pcDNA3-T-bet质粒后:①流式细胞仪检测发现,脾脏Th1百分率较哮喘模型组显著升高[(2.29 ± 1.55)% vs. (1.93 ± 1.14)%,P﹤0.05],Th2百分率显著降低[(0.93 ± 0.64)% vs. (1.63 ± 0.59)%];②RT-PCR检测发现脾脏淋巴细胞转录因子T-bet mRNA表达水平显著增加(0.53 ± 0.027 vs. 0.28 ± 0.035,P﹤0.05), GATA-3 mRNA表达水平显著降低(0.24 ± 0.022 vs. 0.58 ± 0.038,P﹤0.05)65377;而pcDNA3空质粒干预后与哮喘模型组比较无显著差异65377;【结论】 气道内转导pcDNA3-T-bet质粒后,可显著上调哮喘小鼠体内T-betmRNA表达,下调GATA-3 mRNA表达,有效改善哮喘小鼠体内Th1/Th2比例失衡,从而抑制哮喘小鼠的气道炎症,为哮喘的T-bet基因治疗提供依据65377;…”
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Structural and functional properties of hydrolyzed/glycosylated ovalbumin under spray drying and microwave freeze drying by Lili Liu, Xiaoning Dai, Huaibin Kang, Yunfeng Xu, Weiming Hao

“…Ovalbumin (OVA), the main protein in egg white, affects most of the functional properties of egg white protein in food processing. …”
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miR-224-5p Attenuates Allergic Responses in Mice with Allergic Rhinitis by Modulating the Th1/Th2 Response by Yuxiao Li, Ran An, Mingjin Wu, Jiayan He, Xiaoguang He

“…Human nasal epithelial cells (HNEpCs) were used to establish an AR cell model induced by Der P1, and C57BL/6 mice were used to establish an AR animal model induced by OVA (ovalbumin). RT-qPCR was used to determine the level of miR-224-5p; western blot analysis was used to determine GATA3; ELISA was used to determine the levels of OVA-specific IgE, IFN-γ, IL-4, IL-5, and IL-13; flow cytometry was used to determine the differentiation of Th1 and Th2 cells; and HE and PAS staining was used to observe the histopathological alterations in the mouse nasal mucosa and spleen. …”
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March1 E3 Ubiquitin Ligase Modulates Features of Allergic Asthma in an Ovalbumin-Induced Mouse Model of Lung Inflammation by Osama A. Kishta, Antoine Sabourin, Leora Simon, Toby McGovern, Maxime Raymond, Tristan Galbas, Abdelilah Majdoubi, Satoshi Ishido, James G. Martin, Jacques Thibodeau

“…Herein, we used an acute model of allergy to compare the asthmatic phenotype of March1-deficient and -sufficient mice immunized with ovalbumin (OVA) and later challenged by intranasal instillation of OVA in the lungs. …”
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Inhibition of Angiogenic Factor Production from Murine Mast Cells by an Antiallergic Agent (Epinastine Hydrochloride) In Vitro by K. Asano, A. Furuta, K. Kanai, S. Sakaue, H. Suzaki, T. Hisamitsu

“…Mast cells (5×105 cells/mL) presensitized with murine IgE specific for ovalbumin (OVA) were stimulated with 10 ng/mL OVA in the presence of various concentrations of EP for 4 hours. …”
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Pyrroloquinoline Quinone Administration Alleviates Allergic Airway Inflammation in Mice by Regulating the JAK-STAT Signaling Pathway by Zhihui Min, Jiebai Zhou, Ruolin Mao, Bo Cui, Yunfeng Cheng, Zhihong Chen

“…In vivo, PQQ was administered to mice with ovalbumin- (OVA-) induced asthma, and lung pathology and inflammatory cell infiltration were assessed. …”
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Development of an Enzyme-Linked Immunosorbent Assay Based on a Monoclonal Antibody for the Rapid Detection of Citrinin in Wine by Xingdong Yang, Yang Qu, Chenchen Wang, Lihua Wu, Xiaofei Hu

“…The immunogens (CIT-COOH-BSA, CIT-H-BSA) and detection antigen (CIT-COOH-OVA, CIT-H-OVA) were synthesised using the active ester method (-COOH) and formaldehyde addition method (-H). …”
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High protein diet increases the risk of allergic sensitization but not asthma in mice through modulation of the cytokine milieu toward Th2 bias by Waleed Al-Herz, MD, Fawaz Azizieh, PhD, Raj Raghupathy, PhD

“…Allergic sensitization was tested by measuring anti-ovalbumin (OVA) IgE, IgG1, and IgG2a antibodies. Cytokine levels were tested by multiplex ELISA in splenocyte supernatants after stimulation. …”
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Murine model for chronic rhinosinusitis: an interventional study by Amr F. Hamour, John JW. Lee, Ewa Wasilewski, Eric Monteiro, John M. Lee, Allan Vescan, Lakshmi P. Kotra

“…Experimental animals (n = 20) were induced a CRS disease state upon receiving intraperitoneal sensitization with ovalbumin (OVA), followed by intranasal OVA with Aspergillus oryzae protease. …”
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γ-Secretase Inhibitor Alleviates Acute Airway Inflammation of Allergic Asthma in Mice by Downregulating Th17 Cell Differentiation by Weixi Zhang, Xueya Zhang, Anqun Sheng, Cuiye Weng, Tingting Zhu, Wei Zhao, Changchong Li

“…GSI ameliorated the development of OVA-induced asthma, including suppressing airway inflammation responses and reducing the severity of clinical signs. …”
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Štetan uticaj prebirnih sječa na podmlađivanje prebirne šume jele i bukve na području Š.G. “Oštrelj” Drinić by Dalibor Nedimović

“…Ovo poboljšanje stanja podmlatka sa udaljavanjem od početka vlake jasno je vidljivo na samoj vlaci i pored vlake, dok je na 10 m od vlake ova progresija slabijeg intenziteta. …”
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OBJETO VIRTUALES DE APRENDIZAJE EN EL MARCO DE LA EDUCACIÓN 4.0: UNA ALTERNATIVA PEDAGÓGICA MEDIADA CON LAS TIC PARA EL LOGRO DE APRENDIZAJES SIGNIFICATIVOS by Wilson Rodríguez

“…Se concluye que en la actualidad existe un sinfín de metodologías para el diseño y construcción de estos recursos, sin embargo, en la producción de OVA, es necesario considerar la intencionalidad didáctica de los contenidos del recurso, el OVA tiene que ser entendido por los usuarios del mismo, es importante reconocer que siempre y cuando se presente una selección adecuada según el nivel de conocimiento de cada estudiante, así mismo se presentara una mayor aprehensión del conocimiento por parte del mismo. …”
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Therapeutic effects of chlorogenic acid on allergic rhinitis through TLR4/MAPK/NF-κB pathway modulation by Xiaoyan Xu, Lei Wang, Guangyao Wu, Xixia Li

“…Additionally, ovalbumin (OVA)-induced AR mice received different doses of CGA, and Toll-like receptor-4 (Tlr4) overexpression was induced. …”
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Diabetes Downregulates Allergen-Induced Airway Inflammation in Mice by Vinicius F. Carvalho, Emiliano O. Barreto, Ana Carolina S. Arantes, Magda F. Serra, Tatiana Paula T. Ferreira, Yago A. P. Jannini-Sá, Cory M. Hogaboam, Marco A. Martins, Patrícia M. R. Silva

“…Diabetes was induced by intravenous injection of alloxan into 12 h-fasted A/J mice, followed by subcutaneous sensitization with ovalbumin (OVA) and aluminum hydroxide (Al(OH)3), on days 5 and 19 after diabetes induction. …”
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