甲型H1N1流感病毒神经氨酸酶基因遗传进化分析

“…【目的】 了解季节性H1N1流感病毒与2009年新型H1N1流感病毒神经氨酸酶(NA)基因的遗传进化关系,探讨甲型H1N1流感病毒的遗传变异规律65377;【方法】 分别从2006年和2009年流感病人标本中分离并鉴定出季节性HIN1流感病毒和新型H1N1流感病毒,用RT-PCR技术扩增了病毒NA基因全序列,并对其分子进化和重要功能位点的遗传变异进行了分析65377; 【结果】 2009年新型H1N1流感病毒与2006年季节性HIN1流感病毒比较,NA基因的同源性较低(77.9% ~ 78.8 %),与世界各地不同年代代表株及WHO推荐的1979 ~ 2010年季节性流感疫苗株比较,NA基因的同源性也较低(78.1% ~ 79.3%),但与WHO推荐的2009年新型H1N1流感疫苗株比较同源性则高达99%以上;系统进化分析结果表明,2009年新型H1N1流感病毒NA基因与欧亚猪流感病毒株A/swine/Belgium/1/1983的亲缘关系最近;并发现自2005年以来季节性H1N1流感病毒NA基因的某些抗原位点和神经氨酸酶活性位点已发生了变异65377;【结论】 2009年新型H1N1流感病毒NA基因可能来源于欧亚猪流感病毒;接种季节性流感疫苗不能对本次流行的新型流感产生有效的免疫保护作用;季节性H1N1流感病毒在流行过程中NA基因已发生了一定的变异,有必要持续跟踪和监测病毒的变异情况65377;…”
Get full text

Adicción a teléfonos inteligentes, ansiedad, depresión y estrés en estudiantes de enfermería mexicanos by Cornelio Bueno-Brito, Eduardo Pérez-Castro, Josefina Delgado-Delgado

“…Resultados: El 46,70% (99) utiliza el teléfono más de 5 horas al día. El 38,20% (81) de los estudiantes presentó adicción a los teléfonos inteligentes. …”
Get full text

Utility of Integrated Analysis of Pharmacogenomics and Pharmacometabolomics in Early Phase Clinical Trial: A Case Study of a New Molecular Entity by Jaeseong Oh, Sojeong Yi, Namyi Gu, Dongseong Shin, Kyung-Sang Yu, Seo Hyun Yoon, Joo-Youn Cho, In-Jin Jang

Get full text

Efeito da burocracia no custo de transação econômica na Pró-Reitoria de Administração da Universidade Federal Rural do Semi-Árido by Fabianny de Sales Guerra, Luciana Batista Sales, Carlos Alano Soares de Almeida, Adriana Martins de Oliveira, Antônio Erivando Xavier Júnior

Get full text

Neonatal outcomes in offspring of mothers with pregestational diabetes: a hospital-based multicentre prospective cohort study protocol by Manu Raj, Vishnu Renjith, Renjitha Bhaskaran, Anju Philip Thurkkada, Radhamany Kunjukutty, Sobha S Nair, Annie Soman, Sethulakshmi Ramachandran

Get full text

Identification of ketamine and norketamine in dried bloodstains on crime-scene surfaces by Risha Jasmine Nathan, Babajide Okeleye, Rabiu Abdullahi, Waliyu Oyebode

“…The GC–MS method was linear in this range with a coefficient of determination, R 2 > 0.99. Recovery of the analytes was comparable (~ 100–120%) between DBS porous and whole blood, whereas it was considerably lower (~ 50%) in DBS non-porous samples due to the incomplete transfer of the stains from the glass into the extraction solvent mixture. …”
Get full text

Construction and validation of risk prediction models for renal replacement therapy in patients with acute pancreatitis by Fei Zuo, Lei Zhong, Jie Min, Jinyu Zhang, Longping Yao

Get full text

Is Weight Loss the Main Driver for A1C Improvement by Glucagon‐Like Peptide 1 (GLP‐1) Receptor Agonists? A 2.5‐Year Analysis in Real‐World Clinical Practice by Marwa Al‐Badri, Shilton Dhaver, Osama Hamdy

“…Methods We retrospectively evaluated 256 patients with T2D who were treated with exenatide (n = 84), dulaglutide (n = 99), or semaglutide (n = 73) for 2.5 years without interruption in real‐world clinical practice. …”
Get full text

Circulating resistin levels and mutation burden of the RETN gene variants predict long-term mortality in a Taiwanese population by Lung-An Hsu, Ming-Sheng Teng, Semon Wu, Mei-Siou Liao, Hsin-Hua Chou, Yu-Lin Ko

“…Participants with all three RETN variants (high mutation burden) also exhibited significantly higher hazard ratios for all-cause mortality and cancer mortality, at 3.99 and 5.55, respectively, compared to those without a high mutation burden. …”
Get full text

Single-cell sequencing analysis reveals cancer-associated pericyte subgroup in esophageal squamous cell carcinoma to predict prognosis by Kai Xiong, Bing Pan, Hao Fang, Ziyou Tao

Get full text

Magnitude and Its Associated Factors of Urinary Tract Infection among Adult Patients Attending Tigray Region Hospitals, Northern Ethiopia, 2019 by Abrha Hailay, Kidane Zereabruk, Guesh Mebrahtom, Woldu Aberhe, Degena Bahrey

Get full text

Severe Guillain-Barré syndrome with concurrent optic neuritis in a pediatric patient: a case report by Xin Xue, Yingcun Bao, Yakun Yu, Qun Li, Mei Liu, Xiaoling Li

Get full text

Análisis de la disponibilidad de agua útil del suelo en plantaciones de kiwi (Actinidia deliciosa A. Chev) del sudeste bonaerense by M. A. DAVID, H.D. LIGIER, E.E. SÁNCHEZ

Get full text

Effect of PCSK9 Inhibitor on Blood Lipid Levels in Patients with High and Very-High CVD Risk: A Systematic Review and Meta-Analysis by Yue Zhang, Yanrong Suo, Lin Yang, Xiaolu Zhang, Qun Yu, Miao Zeng, Wenlan Zhang, Xijuan Jiang, Yijing Wang

“…Compared with treatments without PCSK9 inhibitor, addition of PCSK9 inhibitors (evolocumab and alirocumab) had obvious decreasing effects on the levels of LDL-C [MD = −46.86, 95% CI (−54.99 to −38.72), P<0.00001], TC [MD = −31.92, 95% CI (−39.47 to −24.38), P<0.00001], TG [MD = −8.13, 95% CI (−10.48 to −5.79), P<0.00001], LP(a) [MD = −26.69, 95% CI (-27.93 to −25.44), P<0.00001], non-HDL-C [MD = −42.86, 95% CI (−45.81 to −39.92), P<0.00001], and ApoB [MD = −38.44, 95% CI (−42.23 to -34.65), P<0.00001] in high CVD risk patients. …”
Get full text

Anemia Prevalence and Risk Factors in Two of Ethiopia’s Most Anemic Regions among Women: A Cross-Sectional Study by Gebru Gebremeskel Gebrerufael, Bsrat Tesfay Hagos

Get full text

Experimental study on immiscible and miscible dynamic characteristics of CO2 and crude oil in visual slim tube by Xing Zhang, Dongchen Ma, Ruiming Zhao, Xiaoyu Zhang, Jiajia Feng, Meng Feng

Get full text

Funcionalidad familiar, habilidades sociales y estilos de vida en estudiantes durante la pandemia por la COVID-19 by Palmer José Hernández-Yepez, Fiorella Inga-Berrospi, Maria Guadalupe Chiroque-Becerra, Paola Ramos-Rupay, Pavel Jaime Contreras-Carmona, Mario Valladares Garrido

“…Las mujeres presentaron mejores niveles de estilos de vida saludable (RP: 0,96, IC 95 %: 0,92-0,99, p= 0,034). No se encontró asociación entre la funcionalidad familiar con el nivel de habilidades sociales (RP: 1,17; IC 95 %: 0,56-2,46, p= 0,678) y con estilos de vida saludable (RP: 1,07; IC 95 %: 0,87-1,31, p= 0,548). …”
Get full text

Remarkable improvement in drilling fluid properties with graphitic-carbon nitride for enhanced wellbore stability by Anwar Ahmed, Erum Pervaiz, Iftikhar Ahmed, Tayyaba Noor

“…The increase in Plastic Viscosity, and Apparent Viscosity was 23.8 %, and 38 %, respectively. Shale recovery was 99.6 % in g-C3N4 treated fluid compared to 88 % in the base fluid. …”
Get full text

Prevalence and influencing factors of regular physical activity among community-dwelling adults in Shaanxi province, 2018: an analysis of surveillance data by Yali TAO, Rina SA, Weihua WANG, Meng WU, Rong LIU, Lin QIU

“…Adults with a college degree or higher (22.53%), retired adults (30.99%), those living in urban areas (17.18%), and those with diabetes (16.23%) had higher weighted prevalence rates of regular physical activity. …”
Get full text

奥美拉唑及吗丁啉加中药降逆汤治疗反流性食管炎临床对比观察 by 陈欣童

“…【结果】中西医结合组的疗效(有效率97.5%)明显高于单用中药组(75%)或单用西药组(80%);患者的血清胃动素犤(182.6±43.9)pg/mL犦与胃泌素犤(99.2±31.7)pg/mL犦水平明显低于正常对照组犤(326.6±58.4)pg/mL,(163.1±28.7)pg/mL犦;3种疗法都能升高血清胃动素与胃泌素水平,而以中西医结合组的作用最好,胃动素与胃泌素平均升高110.9pg/mL和37.7pg/mL,优于单用中药组(27.6pg/mL和17.5pg/mL)或单用西药组(74.6pg/mL和21.7pg/mL)。…”
Get full text