INTESTINAL MICROBIOME - A LEADING FACTOR IN HUMAN HEALTH AND DISEASES by E. Blum Hubert

“…Based on modern molecular, genetic, epigenetic microbiologic and biochemical studies it is, on the one hand, possible to identify disease-related point mutations and single nucleotide polymorphisms within genome-wide association analyses (GWAS). …”
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An integrative approach to the delimitation of pseudocryptic species in the Eucyclops speratus complex (Copepoda, Cyclopoida) with a description of a new species by Aleksandr Novikov, Dayana Sharafutdinova, Ekaterina Abramova, Tatyana Mayor, Elena Chertoprud

“…Eucyclops speratus (Lilljeborg, 1901) (Copepoda, Cyclopoida, Cyclopidae) was studied using various methods. Molecular genetic methods (comparison of COI and ITSn molecular markers) have shown that this species represents a species complex, and the following methods were used to search for differences between the species: analysis of qualitative and quantitative characters, linear morphometrics, landmark-based geometric morphometrics, and integumental pore pattern of the cephalothorax. …”
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MALT-lymphoma of the stomach: clinical presentation, diagnostics and treatment by Ye. V. Ledin, A. P. Seryakov, V. L. Astashov

“…Despite the extensive data obtained for the last two decades on clinical and pathomorphologic properties of this disease, its molecular genetic features, optimal medical approach for this nosological entity it is not developed yet. …”
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Comprehensive analysis of the mechanisms conferring resistance to phenamacril in the Fusarium species by Alexander Dumbai Joe, Runze Liu, Xiao Luo, Ruqiya Syed, Farhan Aslam, Zhenying Luo, Zhitian Zheng

“…We examine the molecular, genetic, and environmental factors contributing to this resistance. …”
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Post-mortem and histological lesions in the European minks (Mustela lutreola) induced by spontaneous infection with coronavirus SARS-CoV-2 by I. A. Subbotina, I. N. Gromov

“…Profound and detailed studies of the virus circulation in the European mink population and the clinical manifestations of the disease, comprehensive approaches to the disease diagnosis, including epidemiological studies, clinical and post-mortem examinations, molecular genetic laboratory diagnostics (polymerase chain reaction and full-genome sequencing) contributed to better understanding of the disease features. …”
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Role of autophagy defects and significance of adherent-invasive <i>Escherichia coli</i> in Crohn's disease development by I. V. Mayev, D. N. Andreev, D. V. Rakitina, Yu. P. Baykova

“…To present new data on a role of molecular genetic disorders of innate immunity at Crohn's disease (CD), and to demonstrate the role of adherent-invasive Escherichia coli (AIEC) as the microorganisms potentially involved in CD development.Summary. …”
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Prospects of Using Microbiota Correction Methods in the Treatment of Inflammatory Bowel Disease by A. I. Akinshina, D. V. Smirnova, A. V. Zagainova, V. V. Makarov, S. M. Yudin

“…In recent years, preference has been given to more accurate and faster molecular genetic methods allowing a more detailed study of the key mechanisms by which microbiota affects the intestine in Crohn’s disease (CD) and ulcerative colitis (UC), as well as of the effect of microbial metabolites on their pathogenesis. …”
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Agr-typing of methicillin-susceptible Staphylococcus aureus (MSSA) isolated from non-human primates by V. A. Kalashnikova

“…The present study is devoted to molecular genetic testing of S. aureus isolated from different biological samples taken from monkeys and is based on typing of agr polymorphic locus which acts as a regulator of pathogenic gene expression. …”
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Techniques of blood sampling for detection of African swine fever virus in wild boar and domestic pigs in the field conditions by A. S. Pershin, A. R. Shotin, E. O. Morozova, A. S. Igolkin, O. A. Manuylova, I. V. Shevchenko, А. А. Shevtsov, N. N. Vlasova

“…To collect field samples of biological material from animals for molecular genetic, virological, and serological tests, filter paper, as well as swabs, can be used. …”
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Comparative characterization of Leporipoxvirus members’ reproduction in continuous cell culture by O. G. Lapteva, S. G. Yurkov, I. P. Sindryakova, A. V. Lunitsin

“…Prepared cultures were identified as myxoma virus and Shope fibroma virus with molecular genetic analysis. Tests of the viruses for their antigenic relatedness showed that antibodies against myxoma virus were able to neutralize Shope fibroma virus also. …”
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Determination of damaging SNP’s in SHANK3 gene with in silico methods by İrem Gülfem Albayrak, Şeyma Yektar, Süeda Kaya

“…There is considerable evidence associating SHANK3 mutations to autism spectrum disorder; hence, it is worthwhile to investigate the underlying molecular genetic mechanisms of the disease. Results This research uses in silico approaches such as SIFT, PolyPhen-2, I-Mutant 2.0, and Project HOPE to find harmful SNPs, which are the single nucleotide polymorphisms the most prevalent types of genetic variation in humans, in the SHANK3 gene. …”
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Avian colibacillosis – current aspects by A. O. Gerasimova, O. B. Novikova, A. A. Savicheva

“…Bacteriological, serological and molecular genetic methods are used for the disease diagnosis. …”
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FREQUENCY OF RH-D NEGATIVE & WEAK D IN PAKISTANI POPULATION by Muhammad Usman et al

“…The expression of its antigens is complex, among that Rh-D antigen is the most important antigen because of its high immunogenicity. Molecular genetic of RHD gene revealed that weak D antigen is a Rh-D phenotype that possesses less numbers of D antigen epitopes on surface of red cells. …”
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MOLECULAR TYPING OF METHICILLIN-SUSCEPTIBLE STAPHYLOCOCCUS AUREUS (MSSA), ISOLATED FROM MONKEYS, BASED ON COAGULASE GENE POLYMORPHISM by V. A. Kalashnikova

“…The present study is devoted to the study of the polymorphism of a variable region of the coagulase gene and to the typing of Staphylococcus aureus isolates from monkeys of different species kept at Adler monkey farm. 115 Staphylococcus aureus isolates were studied using phenotypic and molecular genetic methods. Genotyping was performed using PCR, real-time PCR and PCR with subsequent restriction fragment length polymorphism analysis (PCR-RFLP). …”
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Genomic patterns of strain-specific genetic structure, linkage, and selection across fall armyworm populations by Ashley E. Tessnow, Rodney N. Nagoshi, Robert L. Meagher, Todd M. Gilligan, Ben M. Sadd, Yves Carrière, Holly N. Davis, Shelby J. Fleischer, Kelly Richers, John C. Palumbo, Patrick Porter, Jose Carlos Verle Rodrigues, Gregory A. Sword

“…Abstract Background Molecular genetic approaches have become vital to understanding the evolutionary processes that act on insect pest populations. …”
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Direct and Indirect Methods for Studying Human Gut Microbiota by V. T. Ivashkin, O. S. Medvedev, E. A. Poluektova, A. V. Kudryavtseva, I. R. Bakhtogarimov, A. E. Karchevskaya

“…Aim: To review the main methods of intestinal microbiota studying.Key points. Currently, molecular genetic methods are used mainly for basic research and do not have a unified protocol for data analysis, which makes it difficult to implement them in clinical practice. …”
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Polymorphism of renin — angiotensin system genes in progression of liver fibrosis in patients with chronic hepatitis C by O. V. Taratina, T. N. Krasnova, L. M. Samokhodskaya, T. N. Lopatkina, V. A. Tkachuk, N. A. Mukhin

“…Assessment of polymorphism of studied genes was carried out by molecular genetic methods.Results. In CHC patients of «rapidly progressing fibrosis» group in comparison to «slowly progressing fibrosis» group minor A-allele (50,0 and 33,3% respectively, p=0,0126) and «mutant» АА-genotype (27,3 and 11,1%, р=0,0324; OR АА=3,00; 95% CI 1,07-8,45), AGT gene on locus G-6A, as well as minor T-allele (р=0,0407) of AGT gene in M235T locus were significantly more common while MM genotype of M235T polymorphism of AGT gene (20,8 and 44,4%, respectively, p=0,0090; OR MM=0,33; 95%-CI 0,15–0,73) were significant less frequent. …”
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Development and validation of highly sensitive multiplex real-time RT-PCR assay for detection of classical swine fever virus genome by A. S. Sadchikova, A. S. Igolkin, R. S. Chernyshev, A. A. Kozlov, I. S. Kolbin, A. V. Sprygin, D. A. Biryuchenkov, I. A. Chvala, A. Mazloum

“…A set of anti-epidemic measures including mainly preventive vaccination and annual diagnostic monitoring using molecular-genetic and serological methods is required for CSF virus introduction prevention and rapid eradication of potential disease out breaks. …”
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Enzyme-linked immunosorbent assay for post-slaughter diagnosis of bovine leukosis by A. R. Mustafayev, M. O. Baratov

“…Postmortem diagnosis of enzootic bovine leukosis is made on the basis of the results of tests of biological materials from emergently slaughtered or fallen animals using pathomorphological, histological and molecular genetic methods that have some disadvantages. …”
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Genes of Inflammation and Placental Function GWAS Associated with Idiopathic Recurrent Miscarriage in the Kazakh Population by Alexandra Murtazaliyeva, Gulnara Svyatova, Galina Berezina, Gulfairuz Urazbayeva, Aigerim Sadyrbekova

“…Materials and Methods: Molecular genetic studies were performed by TaqMan using a single site-specific amplification and real-time genotyping method in 302 women with iRM and 300 with normal reproduction. …”
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