Protocol to measure spontaneous locomotion, graded exercise oxygen consumption, and cross-sectional area of skeletal muscle cells in zebrafish by Mauricio Castro-Sepulveda, Cassandra Tabasso, Dogan Grepper, Adrien Martinotti, Axel K.F. Aguettaz, Sylviane Lagarrigue, Francesca Amati

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Protocol on utilizing murine gastric cancer organoids for modeling subcutaneous, orthotopic primary, and liver metastatic disease in mice by Joshua Konecnik, Ryan N. O’Keefe, Kiruthiga Raghunathan, Saumya P. Jacob, Megan O’Brien, Anne Huber, Purva Trivedi, Christine Dijkstra, Amr H. Allam, Michael Buchert, Matthias Ernst, Moritz F. Eissmann

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Protocol for the isolation, culture, and transfection of squid primary cells by Yoonji Kim, Hailey M. Tanner, Joshua J.C. Rosenthal, Clifford P. Brangwynne

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Protocol to study inter-tissue communication between the hypothalamus and white adipose tissue and lifespan using a chemogenetic approach in aged mice by Kyohei Tokizane, Shin-ichiro Imai

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Protocol for assessing mobilization of peritoneal B cells to the pre-metastatic omentum in an orthotopic mouse model of ovarian cancer by WonJae Lee, Hironari Akasaka, Honami Naora

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Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants by Shivraj M. Yabaji, Suruchi Lata, Igor Gavrish, Ming Lo, Aoife K. O’Connell, Hans P. Gertje, Colleen E. Thurman, Nicholas A. Crossland, Lester Kobzik, Igor Kramnik

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Protocol to study in vivo organ-specific migration of apoptotic splenocytes in mice with tumor and immune checkpoint inhibitor-induced colitis by Lukas M. Braun, Robert Zeiser

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Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice by Jiameng Sun, Brian J. DeBosch

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Protocol for inducing epidermis-specific gene deletion and establishing a UVB-irradiated skin inflammation model by Ye Liu, George L. Sen

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Protocol to generate genetically engineered organoid-initiated mouse models of esophageal cancer by Jian Wang, Jiajia Du, Xiangmeng Luo, Linjie Guo, Xiang Li, Ziling Dong, Zhenghao Lu, Xudong Wan, Lu Wang, Hongyu Liu, He Li, Ping Tan, Yanhong Ji, Yuan Li, Yaxin Li, Yushang Yang, Yong Yuan, Longqi Chen, Bing Hu, Yu Liu, Feifei Na, Chong Chen

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Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining by Beatriz Maio, Nagore Elu, Cristina Martinez-Gonzalez, Emily K. Osterweil, Susana R. Louros

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Whole-cell patch clamp and extracellular electrophysiology recordings in mouse brain slices by Huimin Chen, Xiangyi Shi, Fuzheng Guo

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Protocol for in vivo recording of neural activity in deep structures of mice brain via gradient lenses by calcium imaging by Stanislav Cherepanov, Patrice Mollard, Agnes O. Martin

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Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig by Yasmin Eckstein, Barbara Kessler, Arne Hinrichs, Istvan Novak, Anne von Thaden, Timo Lorenzen, Birgit Rathkolb, Armin Scholz, Andreas Blutke, Sietse-Jan Koopmans, Martin Hrabĕ de Angelis, Berit Østergaard Christoffersen, Eckhard Wolf, Simone Renner

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Protocol for microinjection of rapamycin into the zebrafish habenula by Olga Doszyn, Tomasz Dulski, Justyna Zmorzynska

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Protocol for extracellular recordings of mechanically stimulated pentascolopidial chordotonal neurons in Drosophila larvae by Marek B. Körner, Michal Mitro, Dmitrij Ljaschenko, Nicole Scholz

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Protocol to computationally average confocal images of zebrafish lymphatic vessels to identify phenotypes by Hannah Arnold, Virginia Panara, Amin Allalou, Katarzyna Koltowska

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Protocol for assessing the structural architecture, integrity, and cellular composition of murine bone ex vivo by Jonathan W. Lewis, Kathryn Frost, Georgiana Neag, Holly Adams, Emily Powell, Orla Gallagher, Ilaria Bellantuono, Amy J. Naylor, Helen M. McGettrick

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Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model by Tommy Chastel, Serena Filiberti, Stefania Mitola, Roberto Ronca, Andrei Turtoi, Michela Corsini

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Protocol for quantifying xenografted human cancer cells in zebrafish larvae using Cellpose by Karina Köpke, Carla-Sophie Lembke, Nynke Oosterhof, Emma S.C. Dijkstra, Judith T.M.L. Paridaen

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