microRNA-320d通过PBX3抑制子宫内膜癌JEC细胞上皮-间质转化功能 by 龚凤球, 姚书忠

“…【目的】研究microRNA-320d（miR-320d）对子宫内膜癌JEC细胞上皮-间质转化功能的影响及其相关机制。【方 法】 JEC子宫内膜癌细胞株分别转染miR-320d mimics和negative control mimic，分别作为M320d、NCM组，并设立未转染对 照control组，采用RT-PCR法检测各组细胞miR-320d含量。…”
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MicroRNA-208a Dysregulates Apoptosis Genes Expression and Promotes Cardiomyocyte Apoptosis during Ischemia and Its Silencing Improves Cardiac Function after Myocardial Infarction by Hasahya Tony, Kai Meng, Bangwei Wu, Aijia Yu, Qiutang Zeng, Kunwu Yu, Yucheng Zhong

“…Aims. miR-208a is associated with adverse outcomes in several cardiac pathologies known to have increased apoptosis, including myocardial infarction (MI). …”
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Regulation of innate immune response by miRNAs up-regulated in Stevens-Johnson syndrome with severe ocular complications by Mayumi Ueta, Hiromi Nishigaki, Hokoru Yoshioka, Shigeru Kinoshita, Chie Sotozono

“…We analyzed plasma samples from 100 patients with chronic stage SJS/TEN with SOC and 92 healthy controls to examine the expression levels of eight specific miRNAs (let-7a-5p, let-7d-3p, let-7e-5p, miR-146a-5p, miR-130a-3p, miR-151a-3p, miR-151a-5p, miR-27b-3p) using quantitative RT-PCR (RT-qPCR). …”
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Plasma miRNAs in polycystic ovary syndrome drive endometrial cancer progression: insights into molecular pathways and therapeutic targets by Xuedan Lai, Ling Wu, Peihong Lin, Lijiao You, Jianwen Ye

“…Twelve of these miRNAs were associated with UCEC overall survival, with miR-142, miR-424, and miR-331 acting as protective factors, while the remaining 9 miRNAs were identified as risk factors. …”
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Evaluating the safety and effectiveness of α-blockers versus mirabegron for medical expulsive therapy in ureteral calculi: A Systematic review and meta-analysis. by Huilei Yan, Xiaoni Li, Xiaobo Zheng, Yuanshan Cui, Jing Huang, Yan Cheng

“…This meta-analysis evaluated the safety and effectiveness of α-B versus mirabegron (MIR) in treating UC.<h4>Methods</h4>From January 1980 to October 2024, we extensively searched the Pubmed, Web of science, Cochrane and EMBASE databases to identify randomized controlled trials (RCTs) that compared the effectiveness of α-B and MIR in managing UC. …”
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Metastatic melanoma: An integrated analysis to identify critical regulators associated with prognosis, pathogenesis and targeted therapies. by Zeinab Chaharlashkar, Yousof Saeedi Honar, Meghdad Abdollahpour-Alitappeh, Sepideh Parvizpour, Abolfazl Barzegar, Effat Alizadeh

“…MiRNAs analysis revealed hsa-miR-181c-5p, hsa-miR-30b-3p, hsa-miR-3680-3P, hsa-miR-4659a-3p, hsa-miR-4687-3P, and hsa-miR-6808-3P could regulate the hub genes, whereas RP11-553K8.5 and SRP14-AS1 were identified as the top significant lncRNA. …”
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Bioinformatics analysis of miRNAs germacrone protection on diabetic nephropathy by Binqi Wang, Wenfang He, Zhixuan Xie, Jinshi Zhang, Yan Ren, Qiang He, Juan Jin

“…Subsequently, the expression level of mmu-miR-542-5p, mmu-miR-149-5p and mmu-miR-196a-2-3p were validated in a validation dataset. …”
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Deciphering the Regulatory Network of Some Key Drought-responsive Genes and microRNAs in Canola by Maryam Pasandideh Arjmand, Naser Farrokhi, Habibollah Samizadeh Lahiji, Mohammad Mohsenzadeh Golfazani

“…The hub genes were regulated by vital drought-responsive miRNAs such as miR9558, miR854, miR172, miR834, miR390, and miR167. …”
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microRNA-21对膀胱癌T24细胞增殖的影响 及对VEGF和AP1蛋白的调节

“…【目的】 研究microRNA-21(miR-21)对膀胱癌T24细胞系中血管内皮生长因子(vascular endothelial growth factor, VEGF)和活化因子蛋白-1(activator protein-1, AP1)及mRNA表达的调节作用,探讨miR-21对膀胱癌T24细胞增殖的影响65377; 【方法】 用人工合成的miR-21相应的双链互补DNA片段,插入pSilencerTM 3.1-H1 neo载体表达载体,经测序鉴定后作为microRNA的表达质粒;用脂质体将miR-21高表达质粒转染进T24膀胱癌细胞,经G418筛选获得阳性克隆65377;用RT-PCR技术和Western blot方法分别检测该细胞系中VEGF和AP1的mRNA和蛋白表达情况65377;四甲基偶氮唑蓝(MTT)法检测膀胱癌T24细胞增殖的变化情况65377;【结果】 Western blot结果显示miR-21能够增加VEGF和AP1蛋白的表达,RT-PCR方法分析显示VEGF和AP1 mRNA水平升高65377;MTT显示转染后细胞的生长速率比未转染的细胞明显加快65377;【结论】 miR-21促进T24膀胱癌细胞增殖, 提示可能与VEGF和AP1 的低表达有关65377;…”
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Genetic Variants of Neurotransmitter-Related Genes and miRNAs in Egyptian Autistic Patients by Ahmed M. Salem, Samira Ismail, Waheba A. Zarouk, Olwya Abdul Baky, Ahmed A. Sayed, Sawsan Abd El-Hamid, Sohair Salem

“…The study also analyzed sequence variations of miR-431 and miR-21. MAOA uVNTR was genotyped by PCR, MAOB and DRD2 polymorphisms were analyzed by PCR-based RFLP, and miR-431 and miR-21 were sequenced. …”
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Mechanism of Inhibition of Inflammation in Osteoarthritis by Tougu Xiaotong Capsule via Regulating Ras-Raf-MEK1/2-ERK1/2 Signaling Pathway by Munan LIN, Xiang SHAO, Limei XU, Xin ZHANG, Zaishi ZHU, Yidan HAN, Xinwei DUAN, Jianmei CHEN, song GAO, Xihai LI

“…Isolated chondrocytes of passage two were divided into blank group, model group(treatment with 10 ng/mL of LPS for 8 h), inhibitor group(treatment with 10 ng/mL of U0126 for 30 min followed by 10 ng/mL of LPS for additional 8 h)and TXC group(treatment with 10 ng/mL of LPS plus 300 μg/mL of TXC for 8 h).Western blot was carried out to detect the expression of Ras, Raf, p-ERK1/2, MEK1/2, MMP-3 and MMP-13.qPCR was used to detect the mRNAs expression of MEK1/2, ERK1/2, MMP-3 and MMP-13, as well as the expression of miR-27b, miR-34a and miR-146a.Results:1)The cytoplasm of isolated cells(P2)from the cartilage of knee joint of 4-week-old SD male rats was stained brown by typeⅡcollagen immunohistochemical staining as compared with the negative control cells, which indicated that the extracted cells were chondrocytes.2)The expression of Ras, Raf, MEK1/2, MMP-3, MMP-13 in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05), but the expression of p-ERK1/2 had no significant difference(<italic>P</italic>&gt;0.05);the expression of Ras, Raf, p-ERK1/2, MEK1/2, MMP-3, MMP-13 in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05), but the expression of MEK1/2 in the TXC group had no significant difference compared to that in the model group(<italic>P</italic>&gt;0.05).3)The mRNA expression of MEK1/2, ERK1/2, MMP-3 and MMP-13 in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01);their expression in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05).4)The expression of miR-27b in the model group was significantly lower than that in the blank group(<italic>P</italic>&lt;0.01), but its expre-ssion in both the inhibitor group and the TXC group was higher than that in the model group(<italic>P</italic>&lt;0.01);the expression of both miR-34a and miR-146a in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05);however, their expression in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05).Conclusion:Tougu Xiaotong capsule regulates the expression of key factors in Ras-Raf-MEK1/2-ERK1/2 signaling pathway and miR-27b, miR-34a and miR-146a to regulate the inflammatory of chondrocytes, thereby inhibiting osteoarthritis inflammation and protecting articular cartilage.…”
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MicroRNA Deregulation in Anaplastic Thyroid Cancer Biology by Cesar Seigi Fuziwara, Edna Teruko Kimura

“…Upregulation of miRNAs, such as miR-146b, miR-221, and miR-222, is observed in ATC and also in differentiated thyroid cancer (papillary and follicular), indicating that these miRNAs’ overexpression is essential in maintaining tumorigenesis. …”
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The Search of miRNA Related to Invasive Growth of Nonfunctioning Gonadotropic Pituitary Tumors by Joanna Boresowicz, Paulina Kober, Natalia Rusetska, Maria Maksymowicz, Agnieszka Paziewska, Michalina Dąbrowska, Natalia Zeber-Lubecka, Jacek Kunicki, Wiesław Bonicki, Jerzy Ostrowski, Janusz A. Siedlecki, Mateusz Bujko

“…When miRNA profiles of invasive and noninvasive tumors were compared, 29 miRNAs were found differentially expressed. Hsa-miR-184, hsa-miR-181a-2-3p, hsa-miR-93-3p, hsa-miR-574-5p, hsa-miR-185-5p, and hsa-miR-3200-5p showed a potential clinical value according to ROC curve analysis. …”
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Comparison of glimepiride and linagliptin in the treatment of non-alcoholic hepatic disease with type 2 diabetes mellitus by Feng Tian, Yali Guo, Liping Zhou, Qunying Yao, Xiaoyan Liang, Jiaxin Lu, Aiping He, Jie Shen

“…This study aimed to determine whether miR-210 can predict the effects of glimepiride and linagliptin on NAFLD with T2DM. …”
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MicroRNA-3145 as a potential therapeutic target for hepatitis B virus: inhibition of viral replication via downregulation of HBS and HBX by Amrizal Muchtar, Amrizal Muchtar, Daichi Onomura, Daichi Onomura, Dan Ding, Dan Ding, Hironori Nishitsuji, Kunitada Shimotohno, Shunpei Okada, Keiji Ueda, Koichi Watashi, Takaji Wakita, Kei Iida, Kei Iida, Hironori Yoshiyama, Hisashi Iizasa

“…The expression of its precursor, pri-miR-3145, increased in response to the the virus infection, and miR-3145 downregulated the hepatitis B virus S (HBS) antigen and hepatitis B virus X (HBX), thereby inhibiting viral replication. …”
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Clinical Evaluation of the Diagnostic Role of MicroRNA-155 in Breast Cancer by Fatemeh Hosseini Mojahed, Amir Hossein Aalami, Vahid Pouresmaeil, Amir Amirabadi, Mahdi Qasemi Rad, Amirhossein Sahebkar

“…Biochemical markers, including microRNAs (miRs), may facilitate the diagnosis and prognosis of breast cancer. …”
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Mechanism of TGIF1 on glycolipid metabolism disorders in mice with type 2 diabetes by Yuchen Li, Li Tao, Shikai Wang, Fuyan Bai, Liping Zheng, Lijun Hou

“…TGIF1 was enriched on the miR-106b-5p promoter and promoted miR-106b-5p expression. miR-106b-5p inhibited EGR2 expression. miR-106b-5p inhibition or EGR2 overexpression partially reversed the alleviative effect of TGIF1 overexpression on glycolipid metabolism disorders.Conclusion TGIF1 reduces glycolipid metabolism disorders in mice with T2D through the miR-106b-5p/EGR2 axis.…”
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Integrated microRNA and mRNA signatures associated with overall survival in epithelial ovarian cancer. by Joanna Lopacinska-Jørgensen, Douglas V N P Oliveira, Guy Wayne Novotny, Claus K Høgdall, Estrid V Høgdall

“…Several interaction pairs between 6 miRNAs (hsa-miR-126-3p, hsa-miR-223-3p, hsa-miR-23a-5p, hsa-miR-27a-5p, hsa-miR-486-5p, and hsa-miR-506-3p) and 8 mRNAs (ATF3, CH25H, EMP1, HBB, HBEGF, NAMPT, POSTN, and PROCR) were identified and the findings appear to be well supported by the literature. …”
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Three-dimensional Middle Infrared Spectroscopy Study on Crystalline and Amorphous Structures and Thermostability of Corn, Wheat, and Pea Starch by KONG Xiao-mei, DU Lin-nan, JIANG Meng-wei, CHAI Jia-xin, CHANG Mei-ling, ZHANG Yong, CHANG Ming, XU Yuan-yuan

“…The structure and thermal denaturation of three types of starch, including corn starch, wheat starch, and pea starch, were studied using one-dimensional mid infrared (MIR) spectroscopy, temperature dependent one-dimensional MIR spectroscopy and two-dimensional MIR spectroscopy. …”
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Effects of Ginsenoside Biopolymer Nanoparticles on the Malignant Behavior of Non-Small-Cell Lung Cancer by Weizheng Zhou, Chengliang Cai, Hui Shi, Hai Jin, Xiaowei Wang

“…Thirdly, the knockdown of miR-192 significantly inhibited H125 cell proliferation, invasion, and migration and also enhanced H125 cell apoptosis. …”
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