Topologically constrained DNA-mediated one-pot CRISPR assay for rapid detection of viral RNA with single nucleotide resolutionResearch in context by Yanan Li, Ru Xu, Fenglei Quan, Yonghua Wu, Yige Wu, Yongyuan Zhang, Yan Liang, Zhenzhong Zhang, Hua Gao, Ruijie Deng, Kaixiang Zhang, Jinghong Li

“…Rapid and inexpensive at-home testing of viral nucleic acids with single-base resolution remains a challenge. Methods: Topologically constrained DNA ring is engineered as substrates for the trans-cleavage of Cas13a to yield an accelerated post isothermal amplification. …”
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Molecular evidence of the inhibitory potential of melatonin against sodium arsenite toxicity by Ramtin Farhadi, Marzieh Daniali, Maryam Baeeri, Reza Khorasani, Hamed Haghi-Aminjan, Mahdi Gholami, Mahban Rahimifard, Mona Navaei-Nigjeh, Mohammad Abdollahi

“…Due to these findings, this study aimed to evaluate the hypothesis that MLT may ameliorate pancreatic damage caused by exposure to SA. Methods: Forty-eight adult healthy male wistar rats aged 7–8 weeks were divided into eight for this research. …”
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Sex differences and determinants of coronary microvascular function in asymptomatic adults with type 2 diabetes by Jian L. Yeo, Abhishek Dattani, Joanna M. Bilak, Alice L. Wood, Lavanya Athithan, Aparna Deshpande, Anvesha Singh, J.Ranjit Arnold, Emer M. Brady, David Adlam, John D. Biglands, Peter Kellman, Hui Xue, Thomas Yates, Melanie J. Davies, Gaurav S. Gulsin, Gerry P. McCann

“…We aimed to evaluate the sex differences and sex-specific clinical determinants of CMD in adults with T2D without prevalent cardiovascular disease. Methods: Single center pooled analysis of four prospective studies comparing asymptomatic people with T2D and controls. …”
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Wealth-based inequalities in tuberculosis prevalence among households having children and young adults in India: insights from Indian demographic and health surveys (2015–2021) by Saurabh Singh, Quazi Syed Zahiruddin, Sorabh Lakhanpal, Suhas Ballal, Sanjay Kumar, Mahakshit Bhat, Shilpa Sharma, M Ravi Kumar, Yogesh Kumar Dhandh, Sarvesh Rustagi, Mohammed Alissa, Muhammad A. Halwani, Mohammed Garout, Hayam A Alrasheed, Maha F. Al-Subaie, Nawal A. Al Kaabi, Ali A. Rabaan, Sanjit Sah, Muhammed Shabil, Mahalaqua Nazli Khatib, Prakasini Satapathy

“…This study aims to bridge this gap by examining the association between TB and various socio-demographic factors, including socioeconomic status, nutritional status, and environmental conditions. Data and methods Utilizing data from the National Family Health Survey (NFHS) 4th and 5th round (2015-16 and 2019-21), this study conducted a comprehensive cross-sectional analysis. …”
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Modulation of local immunity by the vaginal microbiome is associated with triggering spontaneous preterm birth by Yijia Liang, Yijia Liang, Yijia Liang, Changying Zhao, Yan Wen, Dashuang Sheng, Tiantian Wei, Tianqi Hu, Junhui Dai, Guoping Zhao, Guoping Zhao, Guoping Zhao, Sijie Yang, Sijie Yang, Qinghua Wang, Lei Zhang, Lei Zhang

“…Additionally, it sought to uncover the microbial composition and functional characteristics of immune states linked to preterm birth, and to evaluate the impact of bacterial interactions on the initiation of preterm birth.MethodsUtilizing 16S rRNA sequencing data and local immune factor expression data from a publicly available longitudinal pregnancy cohort, we conducted immunophenotyping through unsupervised clustering of the immune factors. …”
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Associations of Retinal Microvascular Density and Fractal Dimension with Glaucoma: A Prospective Study from UK Biobank by Qi Chen, MD, Suyu Miao, MD, Yuzhe Jiang, MD, Danli Shi, MD, PhD, Weiyun You, MD, Lin Liu, MD, PhD, Mayinuer Yusufu, MTI, Yufan Chen, MD, Ruobing Wang, MD, PhD

“…Subjects: The UK Biobank subjects with fundus images and without a history of glaucoma. Methods: We employed the Retina-based Microvascular Health Assessment System to utilize the noninvasive nature of fundus photography and quantify retinal microvascular parameters including retinal vascular skeleton density (VSD) and fractal dimension (FD). …”
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Accumulation of advanced oxidation protein products aggravates bone-fat imbalance during skeletal aging by Yu-Sheng Huang, Jia-Wen Gao, Rui-Feng Ao, Xin-Yu Liu, Di-Zheng Wu, Jun-Long Huang, Chen Tu, Jing-Shen Zhuang, Si-Yuan Zhu, Zhao-Ming Zhong

“…We examined whether AOPPs accumulation contributes to the bone-fat imbalance during skeletal aging. Methods: Both young and aged mice were employed to assess the changes of AOPPs levels and its contribution to bone-fat imbalance during skeletal aging. …”
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Emergency volunteering willingness and participation: a cross-sectional survey of residents in northern China by Hong Sun, Chaojie Liu, Wei Xu, Ye Li, Qunhong Wu, Lijun Gao, Zheng Kang, Yanhua Hao, Ning Ning, Mingli Jiao, Libo Liang, Yu Cui, Mengli Shi, Chao Liang, Xiaowen Zhao, Jie Fei, Qiuyu Wei, Ming Yi

“…Objectives This study aimed to identify factors that influence people’s willingness to volunteer and participation in emergency volunteering in northern China.Design/Setting This study was conducted in Heilongjiang province in September and October 2014 using a mixed methods approach, which included a cross-sectional questionnaire survey on community residents and in–depth interviews with community residents and relevant organisational managers and officials in relation to emergency responses. …”
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Developmental dysfunction in a preclinical model of Kcnq2 developmental and epileptic encephalopathy by Miaomiao Mao, Nikola Jancovski, Yafit Kushner, Lucas Teasdale, Phan Truong, Kun Zhou, Samuel Reid, Linghan Jia, Ye Htet Aung, Melody Li, Christopher A. Reid, Sean Byars, Ingrid Scheffer, Steven Petrou, Snezana Maljevic

“…In this study, we aimed to characterise a KCNQ2 variant (K556E) found in a female patient with DEE using a heterologous expression system and a knock-in mouse model. Methods: Wild-type KCNQ2 or K556E variant were expressed in Chinese Hamster Ovary (CHO) cells (with or without KCNQ3) and their biophysical properties assessed using patch clamp recordings. …”
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Five-Year Follow-Up of a Slowly Resorbable Biosynthetic P4HB Mesh (Phasix) in VHWG Grade 3 Incisional Hernia Repair by L. M. Van den Dop, MD, M. M. J. Van Rooijen, MD, PhD, T. Tollens, MD, L. N. Jørgensen, MD, PhD, T. S. De Vries-Reilingh, MD, PhD, G. Piessen, MD, PhD, F. Köckerling, MD, PhD, M. Miserez, MD, PhD, M. Dean, MD, F. Berrevoet, MD, PhD, B. Dousset, MD, PhD, H. L. Van Westreenen, MD, PhD, F. Gossetti, MD, PhD, G. W. M. Tetteroo, MD, PhD, A. Koch, MD, PhD, M. F. Boomsma, MD, PhD, J. F. Lange, MD, PhD, J. Jeekel, MD, PhD

“…However, long-term outcomes after resorption are scarce. Methods:. Patients with VHWG grade 3 incisional midline hernia, who participated in the Phasix trial (Clinilcaltrials.gov: NCT02720042) were included by means of physical examination and computed tomography (CT). …”
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Urban environment during pregnancy, cognitive abilities, motor function, and externalizing and internalizing symptoms at 2–5 years old in 3 Canadian birth cohorts by Anne-Claire Binter, Dany Doiron, Martine Shareck, Tona Pitt, Sheila W. McDonald, Padmaja Subbarao, Wiliam D. Fraser, Suzanne C. Tough, Jeffrey Brook, Mònica Guxens

“…Objective: To assess the relationship of urban environmental exposures during pregnancy with cognitive abilities, motor function, externalizing and internalizing symptoms in children. Method: We included 6,279 mother–child pairs from 3 Canadian population-based birth-cohorts (3D Cohort Study in Montreal, Quebec City, and Sherbrooke, AOF Study in Calgary, CHILD Study in Edmonton, Vancouver, Toronto, and Winnipeg). …”
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Interaction between walkability and fine particulate matter on ischemic heart disease: A prospective cohort study in China by Jiayun Zhang, Peng Shen, Yixing Wang, Zihan Li, Lisha Xu, Jie Qiu, Jingjing Hu, Zongming Yang, Yonghao Wu, Zhanghang Zhu, Hongbo Lin, Zhiqin Jiang, Liming Shui, Mengling Tang, Mingjuan Jin, Feng Tong, Kun Chen, Jianbing Wang

“…Nevertheless, their joint impact on IHD warrants further investigation. Methods: This prospective cohort study was performed in Yinzhou, Ningbo, China, comprising 47,516 participants. …”
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Investigating neuropathological correlates of hyperactive and psychotic symptoms in dementia: a systematic review by Giulia Negro, Giulia Negro, Michele Rossi, Camillo Imbimbo, Alberto Gatti, Andrea Magi, Andrea Magi, Ildebrando Marco Appollonio, Ildebrando Marco Appollonio, Alfredo Costa, Alfredo Costa, Tino Emanuele Poloni, Tino Emanuele Poloni

“…This systematic review aims to elucidate the neuropathological basis of the HIDA and psychosis domains, exploring whether distinct proteinopathies and neural circuit dysfunctions are associated with these symptoms.MethodsThe review follows PRISMA guidelines, with a systematic search conducted across MEDLINE, CENTRAL, and EMBASE databases. …”
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Gut microbiota and inflammasome-mediated pyroptosis: a bibliometric analysis from 2014 to 2023 by Hang Zhang, Hang Zhang, Hang Zhang, Tian Zhao, Tian Zhao, Tian Zhao, Juan Gu, Juan Gu, Fushan Tang, Fushan Tang, Fushan Tang, Lei Zhu, Lei Zhu, Lei Zhu

“…Bibliometric analysis provides a comprehensive understanding of this relationship, offering valuable insights into emerging research trends.Materials and methodsLeveraging data spanning from 2014 to 2023 sourced from the Web of Science Core Collection, our analysis was conducted using advanced tools such as SCImago Graphica, VOSviewer, and CiteSpace software. …”
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The impact of maximum cross-sectional area of lesion on predicting the early therapeutic response of multidrug-resistant tuberculosis by Fuzhen Zhang, Yu Zhang, Zilong Yang, Ruichao Liu, Shanshan Li, Yu Pang, Liang Li

“…This study aims to merge the maximum lesion cross-sectional area observed via computed tomography (CT) imaging during treatment to predict therapeutic response. Methods: We retrospectively involved MDR-TB patients who completed 6 months of treatment from two hospitals. …”
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Trends in stroke-related mortality in atrial fibrillation patients in the United States: Insights from the CDC WONDER database by Muhammad Abdullah Naveed, Sivaram Neppala, Himaja Dutt Chigurupati, Muhammad Omer Rehan, Ahila Ali, Hamza Naveed, Bazil Azeem, Rabia Iqbal, Manahil Mubeen, Mashood Ahmed, Ayman R. Fath, Timir Paul, Bilal Munir

“…This study analyzed demographic trends and disparities in mortality rates due to stroke in AF patients aged ≥25 years. Methods: A retrospective analysis was conducted to acquire death data using the Centers for Disease Control and Prevention database from 1999 to 2020. …”
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Analyzing practice pattern in treating partial-thickness rotator cuff tears: a dual perspective from national database and American Shoulder and Elbow Surgeons PARCIAL research gro... by H. Mike Kim, MD, Emily Leary, PhD, Champ L. Baker, 3rd, MD, Leslie A. Barnes, MD, R. Alexander Creighton, MD, Frances Cuomo, MD, Matthew J. DiPaola, MD, Abdullah Foad, MD, James M. Gregory, MD, Brian F. Grogan, MD, Scott G. Kaar, MD, MBA, Eitan M. Kohan, MD, Sumant G. Krishnan, MD, Eddie Y. Lo, MD, John T. Moor, MD, Michael Nguyen, BS, Monica DiFiori, MD, Gabriel Masters, BA, Te Feng Arthur Chou, MD, Riccardo Raganato, MD, Lucas P. Bowen, BS, Jordan J. Harmon, BS, Tessa C. Griffin, BS, Alec E. Winzenried, MD, Evan M. Polce, BS, Cory J. Call, BS, Benjamin Nwadike, MD, Alvin Ouseph, MS, Monia Nazemi, MS, Kyle McCall, BA

“…This study aimed to assess the incidence of surgical repairs for PTRCTs in the United States (U.S.) using the Nationwide Ambulatory Surgery Sample (NASS) database and delineate practice patterns among a group of American Shoulder and Elbow Surgeons (ASES) members. Methods: The NASS database, representing approximately 67% of U.S. ambulatory surgical encounters, was queried to obtain the incidence of PTRCT and FTRCT repairs for the year 2019. …”
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Arbovirus vectors of epidemiological concern in the Americas: A scoping review of entomological studies on Zika, dengue and chikungunya virus vectors. by Reilly Jones, Manisha A Kulkarni, Thomas M V Davidson, RADAM-LAC Research Team, Benoit Talbot

“…The rationale of the review was to identify and characterize entomological studies that have been conducted in the Americas since the introduction of chikungunya virus in 2013, encompassing a period of arbovirus co-circulation, and guide future research based on identified knowledge gaps.<h4>Methods</h4>The preliminary search for this review was conducted on PubMed (National Library of Health, Bethesda, MD, United States). …”
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The Effects of a Red-Light Controllable Nitric Oxide Donor, NORD-1, on Erectile Dysfunction in Rats with Streptozotocin Induced Diabetes Mellitus by Yuji Hotta, Kyoya Oyama, Takuma Yoshida, Naoya Ieda, Taiki Mori, Yasuhiro Horita, Tomoya Kataoka, Yoko Furukawa-Hibi, Susumu Ohya, Hidehiko Nakagawa, Kazunori Kimura

“…In this study, we investigated whether the combination of NORD-1 and red-light irradiation effectively treated ED in streptozotocin (STZ)- treated rats with DM. Materials and Methods: Seven-week-old male Sprague-Dawley rats were used in this study. …”

Clustering patients with COVID-19 according to respiratory support requirements, and its impact on short- and long-term outcome (RECOVID study) by Rosario Menéndez, Raúl Méndez, Ana Latorre, Paula González-Jiménez, Germán Peces-Barba, María Molina-Molina, Pedro Pablo España, Estela García, Angélica Consuegra-Vanegas, Marta María García-Clemente, Carolina Panadero, Juan Marco Figueira-Gonçalves, David De la Rosa-Carrillo, Oriol Sibila, María Dolores Martínez-Pitarch, Nuria Toledo-Pons, Cecilia López-Ramírez, Wanda Almonte-Batista, Abigail Macías-Paredes, Mercedes Villamon, Marisol Domínguez-Álvarez, Eli Nancy Pérez-Rodas, Javier Lázaro, Sarai Quirós, Rosa Cordovilla, Irene Cano-Pumarega, Antoni Torres

“…In addition, we aimed to identify varying profiles of patients according to outcomes and the complexity of respiratory support needed.Methods Multicentre, observational study in 49 Spanish hospitals. …”
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