Eupalinolide B inhibits periodontitis development by targeting ubiquitin conjugating enzyme UBE2D3 by Wenhua Kuang, Ruishen Zhuge, Ping Song, Letai Yi, Shujie Zhang, Ying Zhang, Yin Kwan Wong, Ruixing Chen, Junzhe Zhang, Yuanbo Wang, Dandan Liu, Zipeng Gong, Peili Wang, Xiangying Ouyang, Jigang Wang

“…Furthermore, the direct binding site of EB to UBE2D3 was identified using high‐resolution mass spectrometry and confirmed by experiments. Taken together, EB ameliorates periodontitis by targeting UBE2D3 to suppress the ubiquitination degradation of IκBα, leading to inactivation of nuclear transcription factor‐κB signaling pathway. …”
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Changes in key volatile components associated with leaf quality of Pandanus amaryllifolius Roxb. alongside growth duration by Lu Yan, Ang Zhang, Xiaowei Qin, Huan Yu, Xunzhi Ji, Shuzhen He, Ying Zong, Chunhe Gu, Zhen Feng, Lisong Hu, Zhiqing Lu

“…Gas chromatography-mass spectrometry (GC–MS) was used to identify and analyze VOCs in different leaf positions of Pandan in this study. …”
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Metabolically activated and highly polyfunctional intratumoral VISTA+ regulatory B cells are associated with tumor recurrence in early-stage NSCLC by Domenico Lo Tartaro, Beatrice Aramini, Valentina Masciale, Nikolaos Paschalidis, Francesco Demetrio Lofaro, Anita Neroni, Rebecca Borella, Elena Santacroce, Alin Liviu Ciobanu, Anna Valeria Samarelli, Federica Boraldi, Daniela Quaglino, Alessandra Dubini, Michele Gaudio, Gloria Manzotti, Francesca Reggiani, Federica Torricelli, Alessia Ciarrocchi, Antonino Neri, Federica Bertolini, Massimo Dominici, Pier Luigi Filosso, Franco Stella, Lara Gibellini, Sara De Biasi, Andrea Cossarizza

“…Target liquid chromatography-tandem mass spectrometry confirmed the expression of VISTA on B cells. …”
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Intermittent fasting boosts antitumor immunity by restricting CD11b+Ly6Clow Ly6Glow cell viability through glucose metabolism in murine breast tumor model by Chenghao Fu, Zhehao Liang, Zemiao Niu, Ning Chen, Yuemin Li, Zhenhua Liang, Yanwei Huo, Hao Xi, Rong Wang, Yonghuan Yan, Xiaoru Gano, Mengtian Wang, Yun Huang, Yan Zhang, Mingming Gao, Pin Lü

“…Interestingly, we observed that Ly6Glow G-MDSC sharply decreased after fasting treatment, and the cell surface markers and protein mass spectrometry data showed potential therapeutic targets. …”
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NCAPD3 promotes diffuse large B-cell lymphoma progression through modulating SIRT1 expression in an H3K9 monomethylation-dependent manner by Tiange Lu, Juan Yang, Yiqing Cai, Mengfei Ding, Zhuoya Yu, Xiaosheng Fang, Xiangxiang Zhou, Xin Wang

“…Chromosome spreads, co-immunoprecipitation (co-IP), mass spectrometry (MS), and chromatin immunoprecipitation (ChIP) assays were conducted to untangle the role and mechanism of NCAPD3 in DLBCL. …”
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Elucidating the pharmacological foundations and mechanisms of the Sihai Shuyu formula in treating Graves’ disease through integrated serum metabolomics and network pharmacology wit... by Xiaoju Liu, Xingjia Li, Wenbin Huang, Yifan Cui, Fengyun Cheng, Guofang Chen, Guofang Chen, Xiaodong Mao, Chao Liu, Chao Liu, Shuhang Xu

“…Ultra-High Performance Liquid Chromatography with Quadrupole Time-of-Flight Mass Spectrometry (UPLC-Q-TOF-MS) was used in conjunction with network pharmacology and molecular docking to identify and predict SHSY’s active chemical components and targets. …”
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Gut bacteria Prevotellaceae related lithocholic acid metabolism promotes colonic inflammation by Liping Chen, Zhenghao Ye, Junhua Li, Lijia Wang, Yu Chen, Meiping Yu, Jian Han, Jiangeng Huang, Dongyan Li, Yongling Lv, Kai Xiong, De’an Tian, Jiazhi Liao, Ursula Seidler, Fang Xiao

“…Methods Bile acids in fecal samples from patients with IBD and DSS-induced colitis mice, with and without antibiotic treatment, were analyzed using ultraperformance liquid chromatography-mass spectrometry (UPLC-MS). The composition of the microbiota in fecal samples from IBD patients and DSS-colitis mice was characterized via Illumina MiSeq sequencing of the bacterial 16S rRNA gene V3-V4 region. …”
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Lentivirus‐mediated gene therapy for Fabry disease: 5‐year End‐of‐Study results from the Canadian FACTs trial by Aneal Khan, Dwayne L. Barber, William M. McKillop, C. Anthony Rupar, Christiane Auray‐Blais, Graeme Fraser, Daniel H. Fowler, Alexandra Berger, Ronan Foley, Armand Keating, Michael L. West, Jeffrey A. Medin

“…Globotriaosylceramide (Gb3) and lyso‐Gb3 levels in urine and plasma were assessed by mass spectrometry. qPCR assays measured vector copy number in PB leucocytes. …”
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Indoor, outdoor, and personal exposure to PM2.5 and associated health risk in community-dwelling elderly residents in Fuzhou city, 2021: a cross-sectional study by Shuling KANG, Xin LIN, Kan LIN, Baoying LIU, Chuancheng WU, Jianjun XIANG, Jingwen CHEN, Quan ZHOU

“…PM2.5 concentration was measured with the weighing method; polycyclic aromatic hydrocarbons (PAHs) and metal elemental components in collected PM2.5 were determined by ultra-high performance liquid chromatography and inductively coupled plasma mass spectrometry. The health risk associated with PAHs and metals in PM2.5 was assessed by using the health risk assessment model recommended by the United States Environmental Protection Agency. …”
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The First Large Identification of 3ANX and NX Producing Isolates of <i>Fusarium graminearum</i> in Manitoba, Western Canada by Maria Antonia Henriquez, Srinivas Sura, Sean Walkowiak, David Kaminski, Anne Kirk, Mark W. Sumarah, Parthasarathy Santhanam, Nina Kepeshchuk, Jules Carlson, E. RoTimi Ojo, Pam de Rocquigny, Holly Derksen

“…Mycotoxins were quantified in these samples using mass spectrometry. Using the same rice culture, genomic DNA was isolated, and the <i>Tri1</i> coding sequence along with its flanking regions (upstream and downstream of the <i>Tri1</i> gene) was amplified and sequenced. …”
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Genetic factors shaping the plasma lipidome and the relations to cardiometabolic risk in children and adolescentsResearch in context by Yun Huang, Sara Elizabeth Stinson, Malte Thodberg, Louise Aas Holm, Roman Thielemann, Karolina Sulek, Morten Asp Vonsild Lund, Cilius Esmann Fonvig, Min Kim, Kajetan Trost, Helene Bæk Juel, Trine Nielsen, Peter Rossing, Maja Thiele, Aleksander Krag, Cristina Legido-Quigley, Jens-Christian Holm, Torben Hansen

“…The genetic regulation of lipid species and their impact on cardiometabolic risk during early life remain unexplored. Methods: Using mass spectrometry-based lipidomics, we measured 227 plasma lipid species in 1149 children and adolescents (44.8% boys) with a median age of 11.2 years. …”
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Widespread trace bromine and iodine in remote tropospheric non-sea-salt aerosols by G. P. Schill, K. D. Froyd, K. D. Froyd, D. M. Murphy, C. J. Williamson, C. J. Williamson, C. A. Brock, T. Sherwen, M. J. Evans, M. J. Evans, E. A. Ray, E. A. Ray, E. C. Apel, R. S. Hornbrook, A. J. Hills, J. Peischl, J. Peischl, T. B. Ryerson, C. R. Thompson, I. Bourgeois, D. R. Blake, J. P. DiGangi, G. S. Diskin

“…Using the Particle Analysis by Laser Mass Spectrometry instrument, we found that bromine and iodine are present in 8 %–26 % (interquartile range, IQR) and 12 %–44 % (IQR) of accumulation-mode nSSA, respectively. …”
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Optimization of the Antibacterial Activity of a Three-Component Essential Oil Mixture from Moroccan <i>Thymus satureioides</i>, <i>Lavandula angustifolia</i>, and <i>Origanum major... by Amine Elbouzidi, Mohamed Taibi, Naoufal El Hachlafi, Mounir Haddou, Mohamed Jeddi, Abdellah Baraich, Saad Bougrine, Ramzi A. Mothana, Mohammed F. Hawwal, Waleed A. Alobaid, Abdeslam Asehraou, Bouchra El Guerrouj, Hanae Naceiri Mrabti, Francois Mesnard, Mohamed Addi

“…The chemical profiles of the EOs were analyzed using gas chromatography-mass spectrometry (GC-MS). The antibacterial activity was assessed against <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Pseudomonas aeruginosa</i> using minimum inhibitory concentration (MIC) tests, while antioxidant activity was evaluated through DPPH (2,2-diphenyl-1-picrylhydrazyl), and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assays. …”
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A key technology for synergistic backfilling of coal-based solid waste and high-salinity wastewater by Shuning DONG, Shujiang YU, Xingling DONG, Buqin ZHANG, Xiaoming GUO, Xiaodong WANG, Kai WANG, Shibin ZHU, Boqiang WU, Lei LIU

“…The mechanical properties, microstructures, and heavy metal leaching characteristics of the solidified filling paste were analyzed using the uniaxial compressive strength (UCS) test, scanning electron microscopy (SEM), and inductively coupled plasma-mass spectrometry (ICP-MS). Results and Conclusions The results indicate that the strength of all the solidified filling paste increased over time but gradually decreased with an increase in the quantity of mineral powders added and a decrease in the proportion of cementitious materials. …”
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Elevated mepolizumab levels in patients with severe asthma responsive to 1 year’s mepolizumab treatment by Takayasu Nishimaki, MD, Hitoshi Sasano, MD, PhD, Sonoko Harada, PhD, Tomohito Takeshige, MD, PhD, Yuuki Sandhu, MD, PhD, Yuki Tanabe, MD, Kei Matsuno, MD, PhD, Tetsutaro Nagaoka, MD, PhD, Jun Ito, MD, PhD, Ryo Atsuta, MD, PhD, Mayu Ohuchi, PhD, Shigehiro Yagishita, MD, PhD, Akinobu Hamada, PhD, Kazuhisa Takahashi, MD, PhD, Norihiro Harada, MD, PhD

“…We quantified the serum concentration of mepolizumab at 3, 6, and 12 months after treatment by liquid chromatography coupled with tandem mass spectrometry. Results: Twenty-five adult patients aged 20 years and older with severe asthma were included in the analysis. …”
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RECQL4 affects MHC class II‐mediated signalling and favours an immune‐evasive signature that limits response to immune checkpoint inhibitor therapy in patients with malignant melan... by Sara Egea‐Rodriguez, Renáta Váraljai, Thierry M. Nordmann, Restuan Lubis, Manuel Philip, Florian Rambow, Alexander Roesch, Michael Flaig, Susanne Horn, Raphael Stoll, Fang Zhao, Annette Paschen, Bert Klebl, Ian D. Hickson, Dirk Schadendorf, Matthias Mann, Iris Helfrich

“…Finally, we generated genetically engineered RECQL4 variants and conducted comprehensive multi‐omic profiling, employing techniques such as liquid chromatography with tandem mass spectrometry, to elucidate mechanistic insights. Results We identified RECQL4 as a critical negative regulator of poor prognosis and response to ICI therapy, but also demonstrated its suitability as an independent biomarker in melanoma. …”
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Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibiti... by Hui Luo, Jianhua Chen, Cao Li, Tian Wu, Siyue Yin, Guangping Yang, Yipin Wang, Zhihan Guo, Saifei Hu, Yanni He, Yingnan Wang, Yao Chen, Youqiang Su, Congxiu Miao, Yun Qian, Ruizhi Feng

“…Immunoprecipitation‐mass spectrometry and ultramicro‐quantitative proteomics were performed to identify the differential abundance proteins and affected interactome of TUBB8 protein. …”
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Biological activities of Usnea lethariiformis lichen extracts and UHPLC-ESI-QTOF-MS analysis of their secondary metabolites by Mauricio Piñeiro, Mauricio Piñeiro, Sofía Manrique, Jessica Gómez, Jessica Gómez, Juan Manuel Rodriguez, Patricia Barrera, Duilio Caballero, Miguel A. Sosa, Gabriel Vargas-Arana, Gabriel Vargas-Arana, Alejandro Tapia, Beatriz Lima, Beatriz Lima, Mario J. Simirgiotis

“…The metabolite profiling was obtained using ultra-high-resolution liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS) system. The trypanocidal and nematicidal activities were determined according to standardized protocols. …”
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Site-specific immunoglobulin G N-glycosylation is associated with gastric cancer progression by Tingting Xu, Jianmin Huang, Jiajing Lin, Yuanyuan Liu, Yi Wang, Wenkang Shen, Jianjie He, Shuyun Chen, Xi Zhu, Yuqin Que, Mengting Hu, Yu Chen, Liming Cheng, Honghao He, Xin Liu, Si Liu

“…Methods The IgG N-glycome in GC was characterized using a custom high-throughput orthogonal mass spectrometry approach. Multivariate analysis was employed to identify and assess glycomic alterations. …”
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Exometabolomic exploration of culturable airborne microorganisms from an urban atmosphere by R. Jin, W. Hu, W. Hu, P. Duan, M. Sheng, D. Liu, Z. Huang, M. Niu, L. Wu, J. Deng, P. Fu, P. Fu

“…Molecular compositions of exometabolites were analyzed using ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry. Annotation through the Kyoto Encyclopedia of Genes and Genomes database helped identify metabolic processes. …”
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