Ethyl Acetate Extract of <i>Cichorium glandulosum</i> Activates the P21/Nrf2/HO-1 Pathway to Alleviate Oxidative Stress in a Mouse Model of Alcoholic Liver Disease by Shuwen Qi, Chunzi Zhang, Junlin Yan, Xiaoyan Ma, Yewei Zhong, Wenhui Hou, Juan Zhang, Tuxia Pang, Xiaoli Ma

“…Methods: Ultra-Performance Liquid Chromatography coupled with Quadrupole-Orbitrap Mass Spectrometry (UPLC-Q-Orbitrap-MS) was used to identify CGE components. …”
Get full text

Comparative Proteome Analysis of Epicardial and Subcutaneous Adipose Tissues from Patients with or without Coronary Artery Disease by Yu xing Zhao, Hui juan Zhu, Hui Pan, Xue mei Liu, Lin jie Wang, Hong bo Yang, Nai shi Li, Feng ying Gong, Wei Sun, Yong Zeng

“…Isobaric Tagging for Relative and Absolute Quantitation (iTRAQ) analysis combined with liquid chromatography tandem-mass spectrometry (LC-MS/MS) was performed to identify the differentially expressed proteins. …”
Get full text

Novel Thiosemicarbazone Derivatives from Furan-2-Carbaldehyde: Synthesis, Characterization, Crystal Structures, and Antibacterial, Antifungal, Antioxidant, and Antitumor Activities by Wilfredo Hernández, Fernando Carrasco, Abraham Vaisberg, Evgenia Spodine, Maik Icker, Harald Krautscheid, Lothar Beyer, Carmen Tamariz-Angeles, Percy Olivera-Gonzales

“…The prepared compounds were characterized by spectroscopic studies (FT-IR and NMR) and electrospray mass spectrometry. The molecular structures of 2, 6, 7, and 8 have also been determined by X-ray crystallography. …”
Get full text

A Study of the Relationship between the Polymorphism and Mutation of rs682429 and rs3781590 in the LRP5 Gene and Bone Metabolism in Postmenopausal Type 2 Diabetic Women in Xinjiang by Jun Li, SiYuan Li, HuiRong Zhao, JiaJia Li, Shuang Wang, YanQiu Shi

“…Calcium (Ca), phosphorus (P), alkaline phosphatase (ALP), and clinical biochemical data were determined; haemoglobin A1c (HbA1c) was measured by HPLC; BMD of the femoral neck, hip, and lumbar spine (L1-4) was measured by dual-energy X-ray (DEXA); and the rs682429 and rs3781590 polymorphisms of the LRP5 gene were detected by time-of-flight mass spectrometry (TOF MS). Results. (1) The rs682429 polymorphism of the LRP5 genotype distribution was statistically significant (P<0.05) in group B compared with group A. (2) The triglycerides (TG) of women with the CT/TT genotype (mutant type) were higher than those of women with the CC genotype (wild type) (2.37±1.30 vs. 1.52±0.83, P<0.05) at the rs3781590 site of the LRP5 gene in group D. (3) Multiple linear regression analysis showed that TG (β=0.034, P<0.05) and body mass index (BMI) (β=0.013, P<0.05) were the influencing factors of BMD (L1-4) in T2DM patients. …”
Get full text

Exploring Innovative Approaches for the Analysis of Micro- and Nanoplastics: Breakthroughs in (Bio)Sensing Techniques by Denise Margarita Rivera-Rivera, Gabriela Elizabeth Quintanilla-Villanueva, Donato Luna-Moreno, Araceli Sánchez-Álvarez, José Manuel Rodríguez-Delgado, Erika Iveth Cedillo-González, Garima Kaushik, Juan Francisco Villarreal-Chiu, Melissa Marlene Rodríguez-Delgado

“…Thermal analytical methods, including pyrolysis gas chromatography–mass spectrometry (Py-GC-MS), provide compositional insights but are destructive and limited in morphological analysis. …”
Get full text

Targeting deubiquitinase USP7-mediated stabilization of XPO1 contributes to the anti-myeloma effects of selinexor by Junying Wang, Mengping Chen, Jinxing Jiang, Yike Wan, Xin Li, Minyue Zhang, Fei Xiao, Lu Zhong, Hua Zhong, Zhaoyu Qin, Jian Hou

“…Immunoprecipitation coupled with mass spectrometry (IP-MS), bioinformatics analysis and ubiquitination assays, were employed to identify the molecular targets responsible for SEL-induced degradation of XPO1. shRNA-mediated knockdown assays and small molecule inhibitors of USP7 were utilized to disrupt the function of USP7. …”
Get full text

The effects of Fraxini cortex and Andrographis herba on Escherichia coli-induced diarrhea in chicken by Yunying Wang, Zhenwei Sheng, Huicong Li, Xuewen Tan, Yingqiu Liu, Weimin Zhang, Wuren Ma, Lin Ma, Yunpeng Fan

“…The research also investigated the impact of these formulas on gut microbiota and serum metabolites in chickens, using 16S rRNA sequencing technology and metabolomics. Mass spectrometry technology and network pharmacology were used to analyze the optimal TCM formula corroborated by molecular docking and qPCR for further explore mechanism exploration. …”
Get full text

Comparative analysis of anticoagulant influence on PMI estimation based on porcine blood metabolomics profile measured using GC-MS by Patrycja Mojsak, Paulina Samczuk, Paulina Samczuk, Paulina Klimaszewska, Michal Burdukiewicz, Michal Burdukiewicz, Jaroslaw Chilimoniuk, Krystyna Grzesiak, Krystyna Grzesiak, Karolina Pietrowska, Justyna Ciborowska, Anna Niemcunowicz-Janica, Adam Kretowski, Adam Kretowski, Michal Ciborowski, Michal Ciborowski, Michal Szeremeta

“…Therefore, in this study, we employed gas chromatography-mass spectrometry (GC-MS)-based metabolomics to assess post-mortem changes in porcine blood samples collected with and without the addition of anticoagulant (EDTA). …”
Get full text

GC/MS Fatty Acid Profile of Marine-Derived Actinomycetes from Extreme Environments: Chemotaxonomic Insights and Biotechnological Potential by Marlene B. Cunha, André F. Jorge, Maria João Nunes, Joana R. Sousa, Maria João Lança, Marco Gomes da Silva, Susana P. Gaudêncio

“…Fatty acid methyl esters (FAMEs) were prepared from the ethyl acetate lipidic extracts of these strains and analyzed by gas chromatography–mass spectrometry (GC/MS), with FA identification performed using the NIST library. …”
Get full text

Effects of dietary l-carnosine supplementation on the growth, intestinal microbiota, and serum metabolome of fattening lambs by Yaxuan Meng, Yaxuan Meng, Yaxuan Meng, Tingting Xian, Tingting Xian, Guolei Kang, Hongna Wang, Tao Feng, Tao Feng, Tao Feng

“…The fecal microbiota was analyzed using 16S rRNA sequencing, and serum metabolites were analyzed using liquid chromatography–tandem mass spectrometry. Spearman correlation analysis was employed to assess the associations between intestinal microbiota and serum metabolite biomarkers. …”
Get full text

The compensatory enrichment of sphingosine-1-phosphate on HDL in FSGS enhances the protective function of glomerular endothelial cells compared to MCD by Yunfeng Xiong, Qiuping Ye, Lifang Liu, Wanjun Lin, Yonggen Liao, Ruiyu Gao, Jiaming Xu, Xinyu Zhang, Ruoyan Chen, Sihui Chen, Qiaoling Chen, Lixin Wei

“…Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyzed S1P concentrations, while real-time quantitative PCR evaluated the expression of enzymes involved in S1P metabolism. …”
Get full text

Source distribution, ecological risks, and controlling factors of heavy metals in river sediments: Receptor model-based study in a transboundary river basin by Abu Reza Md Towfiqul Islam, Abu Hena Md Fazla Rabbi, Amit Hasan Anik, Rahat Khan, Md Abdullah Al Masud, Grara Nedjoud, Abubakr M. Idris, Md Naimur Rahman, Venkatramanan Senapathi

“…The current investigation encompasses the analysis of twenty-seven sediment samples, utilizing inductively coupled plasma mass spectrometry, in the transboundary river basin of Bangladesh, specifically the Teesta River. …”
Get full text

Rainy and Dry Seasons Are Relevant Factors Affecting Chemical and Antioxidant Properties of Meliponini Honey by Flavia C. Lavinas, Brendo A. Gomes, Marcos V. T. Silva, Raissa Lima, Suzana G. Leitão, Mirian R. L. Moura, Rosineide C. Simas, Renata F. Barbosa, Fabricio O. Silva, Carla S. Carneiro, Igor A. Rodrigues

“…The mineral content was assessed via TXRF spectroscopy, and chemical fingerprinting was conducted using mass spectrometry. Chemometric tools were used for the samples’ discriminating analyses, including Principal Component Analysis (PCA) and Partial Least Squares–Discriminant Analysis (PLS-DA). …”
Get full text

Metabolic profiling of Perilla leaves of different colors: insights into metabolite variation and bioactive compound distribution by Senouwa Segla Koffi Dossou, Qianchun Deng, Feng Li, Lei Wang, Nanjun Jiang, Habtamu Kefale, Rong Zhou, Donghua Li, Meilian Tan, Linhai Wang

“…Thus, in this study, we applied LC-QqQ-MS (liquid chromatography triple quadrupole tandem mass spectrometry)-based widely targeted metabolic profiling to investigate variation in the metabolite profiles of green (PF1), green/purple (PF2) and purple (PF3) Perilla leaves. …”
Get full text

Prevalence of psychoactive substance use among acutely hospitalised patients in Oslo and Moscow: a cross-sectional, observational study by Stig Tore Bogstrand, Thomas Berg, Anners Lerdal, Danil Gamboa, Benedicte Jørgenrud, Evgeny A Bryun, Vigdis Vindenes, Evgenya A Koshkina, Aleksei V Nadezhdin, Saranda Kabashi, Elena J Tetenova, Anna Armika Tussilago Nyman, Alexey J Kolgashkin, Aleksei E Petukhov, Sergey N Perekhodov, Elena N Davydova, Gudmund Nordby

“…The study sites did not admit patients with surgical conditions and/or injuries.Primary and secondary outcomes The presence of psychoactive substances was determined through blood analysis using liquid chromatography-mass spectrometry. Secondary outcomes comprised demographic data (including age, gender, employment and marital status), degree of psychological distress, concurrent alcohol use, and self-reported alcohol, psychoactive drug and illicit substance use.Results 32.3% in Oslo and 12% in Moscow were positive for one or more psychoactive medicinal drugs (benzodiazepines, z-hypnotics, opioids or barbiturates). …”
Get full text

Composition of antifungal volatile organic compounds in Sextonia rubra fruit by molecular networks by Levasseur, Marceau, Santerre, Cyrille, Segret, Juliette, Elie, Nicolas, Genty, Christophe, Estevez, Yannick, Amusant, Nadine, Houël, Emeline, Eparvier, Véronique, Touboul, David

“…Hydrodistillation of its fruit was performed for the first time and the resulting hydrolate was analyzed by headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) together with the dried fruit. …”
Get full text

Leupaxin promotes hepatic gluconeogenesis and glucose metabolism by coactivation with hepatic nuclear factor 4α by Xiaomin Luo, Fang Liu, Lijun Zhu, Caizhi Liu, Ruhui Shen, Xiaoyin Ding, Yufan Wang, Xiaofang Tang, Yongde Peng, Zhijian Zhang

“…Methods: We initially used protein pull-down assays, mass spectrometry and luciferase assays to identify the coactivator that interacts with HNF4α in gluconeogenesis. …”
Get full text

The effects of ultra-selective beta1-antagonism on the metabolic and cytokine profile in septic shock patients receiving noradrenaline: a sub-investigation from the STRESS-L Random... by Jarrod L. Thomas, Kirsty C. McGee, Anower Hossain, Gavin D. Perkins, Anthony C. Gordon, Duncan Young, Danny McAuley, Mervyn Singer, Ranjit Lall, Tina Kramaric, Janet M. Lord, Tony Whitehouse, Luis A. J. Mur, for the STRESS-L. collaborators

“…Metabolite fingerprinting was carried out by flow infusion electrospray ionisation high-resolution mass spectrometry and metabolomic data were analysed using the R-based platform MetaboAnalyst. …”
Get full text

Investigating the neuroprotective effects of Dracocephalum moldavica extract and its effect on metabolomic profile of rat model of sporadic Alzheimer's disease by Marjan Talebi, Seyed Abdulmajid Ayatollahi, Mohammad Ali As’Habi, Farzad Kobarfard, Mona Khoramjouy, Farzaneh Niki Boroujeni, Mehrdad Faizi, Alireza Ghassempour

“…Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) was used to assess metabolite changes in various brain regions. …”
Get full text

The Identification and synthesis of metabolites of 4-(5-methyl-1,3,4-oxadiazole-2-yl)-benzenesulfonamide by Alexander L. Khokhlov, Ilya I. Yaichkov, Anton A. Shetnev, Valeria A. Panova, Julia A. Efimova, Sergey A. Ivanovskiy, Mikhail K. Korsakov, Nikita N. Volkhin, Sergey S. Petukhov

“…The structure of the obtained substances was confirmed by NMR spectroscopy and high-resolution mass spectrometry. At the final stage, animal biological fluids and model samples with the addition of the synthesized compounds were analyzed using HPLC-MS/MS to establish the structure of metabolites.Results and Discussion: N-hydroxy-4-(5-methyl-1,3,4-oxadiazole-2-yl)-benzenesulfonamide, 4-(5-(hydroxymethyl)-1,3,4-oxadiazole-2-yl)-benzenesulfonamide and 4-(5-methyl-1,3,4-oxadiazole-2-yl)-benzenesulfonic acid were identified and synthesized. …”
Get full text