Biocontrol of gray spot disease on Polygonatum cyrtonema Hua by Bacillus velezensis GX1 by L. Sun, G.L. Deng, H.L. Wu, Q. He, S.H. Hu, J.Y. Li, X.Y. Li, S. Meng, A.N. He, Y.Q. Tian, X. Chen, S.H. Li

“…Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis revealed that strain GX1 produced significantly higher levels of surfactin A, surfactin B and plipastatin, relative to strain GX8, an ineffective strain. …”
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Advanced tissue technologies of blood-brain barrier organoids as high throughput toxicity readouts in drug development by Luisa Bell, Claire Simonneau, Chiara Zanini, Elena Kassianidou, Christelle Zundel, Rachel Neff, Bernd Steinhuber, Marco Tecilla, Alex Odermatt, Roberto Villaseñor, Nadine Stokar-Regenscheit

“…Here, we developed an adapted set of orthogonal image-based tissue methods including hematoxylin and eosin staining (HE), immunohistochemistry (IHC), multiplex immunofluorescence (mIF), and Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI) to validate CIVMs for drug toxicity assessments. …”
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Puerariae Radix protects against ulcerative colitis in mice by inhibiting NLRP3 inflammasome activation by Yu Ga, Yuanyuan Wei, Qingyu Zhao, Yimeng Fan, Yannan Zhang, Zhifang Zhang, Sijia Hao, Lixia Wang, Zhifen Wang, Jinlong Han, Shuang Wu, Zhihui Hao

“…The chemical constituents of PR via ultra-performance liquid chromatography/tandem mass spectrometry and identifi ed potential PR and UC targets using a network pharmacology (NP) approach were obtained to guide mouse experiments. …”
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Development of Ketoprofen Impurity A (1-(3-Benzoylphenyl)ethanone) as a Certified Reference Material for Pharmaceutical Quality Control by Nikolay A. Shulga, Vladimir I. Gegechkori, Natalya V. Gorpinchenko, Valery V. Smirnov, Sergey P. Dementyev, Galina V. Ramenskaya

“…The obtained product was purified using preparative chromatography and characterised by infrared spectroscopy (IR), 1H and 13C nuclear magnetic resonance (NMR), and mass spectrometry. The structure was verified using primary research methods to confirm its identity as the target product. …”
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Relationship between prenatal metals exposure and neurodevelopment in one-year-old infants in the CLIMB study by Xiao-Yuan Fan, Xian-Shu Lin, Bing-Rui Yang, Han-Wen Zhang, Feng Tang, Jia-Jia Tang, He-Bin Chi, Toby Mansell, Noora Kartiosuo, Yin-Yin Xia, Ting-Li Han, Hua Zhang, Philip Baker, Richard Saffery

“…The concentrations of 21 metallic elements and 2 metalloids in umbilical cord blood (UCB) serum were measured using inductively coupled plasma mass spectrometry (ICP-MS). Neurodevelopment was measured using Chinese version of Bayley Scales of Infant Development (BSID) for the Psychomotor Development Index (PDI) and the Mental Development Index (MDI). …”
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Quantitative proteomics and targeted metabolomics reveal the role of Cassia siamea L. extract in the response to high-fat diet-induced obesity in Drosophila by Ananya Dechakhamphu, Nattapong Wongchum, Theeraphan Chumroenphat, Alongklod Tanomtong, Sirithon Siriamornpun

“…Methods: CSE was obtained via extraction from C. siamea wood with 70 % ethanol and its phytochemical content was analyzed by gas chromatography–mass spectrometry (GC‒MS) and high-performance liquid chromatography (HPLC). …”
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Robust collection and processing for label-free single voxel proteomics by Reta Birhanu Kitata, Marija Velickovic, Zhangyang Xu, Rui Zhao, David Scholten, Rosalie K. Chu, Daniel J. Orton, William B. Chrisler, Tong Zhang, Jeremy V. Mathews, Benjamin M. Bumgarner, Demirkan B. Gursel, Ronald J. Moore, Paul D. Piehowski, Tao Liu, Richard D. Smith, Huiping Liu, Clive H. Wasserfall, Chia-Feng Tsai, Tujin Shi

“…Abstract With advanced mass spectrometry (MS)-based proteomics, genome-scale proteome coverage can be achieved from bulk tissues. …”
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The effects of age and sex on reference intervals for cobalamin, homocysteine, and serum and urinary methylmalonic acid in healthy adult dogs by Anna‐Lena Proksch, Sophia Schaefer, Vanessa Dreller, Judith Langenstein, Ralph Fingerhut, Natali Bauer, Andreas Moritz

“…Cobalamin and markersB12met were determined using chemiluminescence immunoassay (cobalamin) and liquid chromatography/tandem mass spectrometry (homocysteine, sMMA, uMMA:crea). In dogs with outlying data, changes in health, markersB12met, and onset of gastrointestinal signs were reevaluated after 9‐15 months. …”
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Alterations of gray matter volume and functional connectivity in patients with cognitive impairment induced by occupational aluminum exposure: a case-control study by Huaxing Meng, Bo Liu, Xiaoting Lu, Yan Tan, Shanshan Wang, Baolong Pan, Hui Zhang, Qiao Niu

“…Plasma aluminum levels were measured using inductively coupled plasma-mass spectrometry. Cognitive function was assessed using the Montreal Cognitive Assessment (MoCA) and an auditory-verbal learning test (AVLT). …”
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Technical note: “U–Th Analysis” – open-source software dedicated to MC-ICP-MS U-series data treatment and evaluation by I. K. Kerber, F. Kontor, A. Mielke, A. Mielke, S. Warken, S. Warken, N. Frank

“…<p>We present our stand-alone data analysis application for <span class="inline-formula"><math xmlns="http://www.w3.org/1998/Math/MathML" id="M1" display="inline" overflow="scroll" dspmath="mathml"><mrow class="chem"><msup><mi/><mn mathvariant="normal">230</mn></msup><mrow class="chem"><mi mathvariant="normal">Th</mi><mo>/</mo><mi mathvariant="normal">U</mi></mrow></mrow></math><span><svg:svg xmlns:svg="http://www.w3.org/2000/svg" width="43pt" height="15pt" class="svg-formula" dspmath="mathimg" md5hash="3e17e119ba9c72e5f696f4fcc9cdcb4a"><svg:image xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="gchron-7-1-2025-ie00001.svg" width="43pt" height="15pt" src="gchron-7-1-2025-ie00001.png"/></svg:svg></span></span> dating with multi-collector inductively coupled plasma mass spectrometry (MC-ICP-MS). The Python-based algorithm is equipped with a graphical user interface (GUI) and comprises raw data treatment, corrections, age calculation, and error estimation. …”
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Parasitic infections during pregnancy in Gabon affect glycosylation patterns of maternal and child antibodies by Yabo J. Honkpehedji, Anna O. Kildemoes, Koen A. Stam, Dieu L. Nguyen, Tom Veldhuizen, Angela van Diepen, Meral Esen, Peter G. Kremsner, Manfred Wuhrer, Ayôla A. Adegnika, Cornelis H. Hokke, Maria Yazdanbakhsh

“…IgG Fc glycosylation was measured by liquid chromatography-mass spectrometry, determining fucosylation, galactosylation, sialylation, bisection, and sialylation per galactose (SA/gal). …”
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Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice by Han Wang, Lizi Zhang, Xing Chen, Lingzi Hong, Junjie Zhao, Wen Qian, Lam Khue Pham, Belinda Willard, Xiaoxia Li, Katarzyna Bulek, Xiao Li

“…Adipocyte-specific Steap4-deficient mice exhibited increased fat mass and severe insulin resistance in our high-fat diet model. Mass spectrometry identified two classes of Steap4 interactomes: mitochondrial proteins and proteins involved in splicing. …”
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Interaction Between Malat1 and miR-499-5p Regulates Meis1 Expression and Function with a Net Impact on Cell Proliferation by Salma A. Fahim, Manon Ragheb, Ibrahim Hassan Fayed, Aya Osama, Ahmed Karam, Sameh Magdeldin, Rana Metwale, Mohamed Dief Allah Abdalmoneam Elsayed, Ahmed Abdellatif, Hesham A. Sadek, Shereen Ahmed El Sobky, Nada El-Ekiaby, Injie Omar Fawzy, Ahmed Ihab Abdelaziz

“…Enrichment analyses on proteins identified via mass spectrometry after manipulating Malat1, miR-499-5p, or Meis1 revealed a multitude of differentially expressed proteins related to cell cycle, cell division, and key pathways like Wnt and mTOR, essential for cell proliferation. …”
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Salivary proteomics profiling reveals potential biomarkers for chronic kidney disease: a pilot study by Bianca Uliana Picolo, Nathália Rabello Silva, Mário Machado Martins, Hebréia Oliveira Almeida-Souza, Letícia Cristina Machado de Sousa, Richard Costa Polveiro, Luiz Ricardo Goulart Filho, Robinson Sabino-Silva, Vivian Alonso-Goulart, Luciana Saraiva da Silva

“…Proteomics using saliva is a key strategy for identifying potential disease biomarkers.MethodsWe analyzed the untargeted proteomic profiles of saliva samples from 20 individuals with end-stage kidney disease (ESKD) (n = 10) and healthy individuals (n = 10) using liquid chromatography–tandem mass spectrometry (LC–MS/MS) to identify potential biomarkers for CKD. …”
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Antibiotic Resistance and Molecular Epidemiological Characteristics of Streptococcus agalactiae Isolated from Pregnant Women in Guangzhou, South China by Zhaomin Cheng, Pinghua Qu, Peifeng Ke, Xiaohan Yang, Qiang Zhou, Kai Lan, Min He, Nannan Cao, Sheng Qin, Xianzhang Huang

“…Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) mass peak analysis was performed to discriminate the specific sequence types (STs). …”
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Gut Microbiome and Metabolite Characteristics Associated With Different Clinical Stages in Non-Small Cell Lung Cancer Patients by Liu F, Lu X, Tang M, Chen Y, Zheng X

“…Subsequently, they performed 16S rRNA gene amplification sequencing and untargeted gas/liquid chromatography-mass spectrometry metabolomics analysis.Results: The study revealed that the alpha-diversity of the gut microbiome in NSCLC patients at different stages did not exhibit statistically significant differences. …”
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The effects of Thymus capitatus essential oil topical application on milk quality: a systems biology approach by Ralph Nehme, Chiara Gini, Elise Vanbergue, Sergine Even, Filippo Biscarini, Sonia Andrés, Lucie Rault, Faustine Noel, Valerie Hardit, Said Bouhallab, David M. Pereira, Riadh Ksouri, Philippe Roussel, Secundino López, Paola Cremonesi, Bianca Castiglioni, Donatella Caruso, Fiorenza Faré, Manuela Fontana, Fabrizio Ceciliani, Latifa Abdennebi-Najar

“…This study aimed to investigate the impact of Thymus capitatus essential oil (TCEO), known for its high antibacterial and antioxidant properties, on milk microbiota using 16S rRNA sequencing, the lipidomic profile via liquid chromatography-quadrupole time-of-flight mass spectrometry, udder skin microbiota, and inflammatory biomarkers of dairy cows at the end of lactation. …”
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Phytochemical Profiling, GC-MS Analysis and Antibacterial Activity of Sida acuta leave Extracts Against Helicobacter pylori by Kamoldeen Ajijolakewu, Ayobami Elias Ajadi, S A Ayoola, Sheriffdeen Issa Bale, Taiwo Ayodeji Sorunke, Saheed Abayomi Onifade, Tomisin Oyawoye

“…This research assessed the phytochemical profile, performed Gas Chromatography-Mass Spectrometry (GC-MS) analysis, and evaluated the antibacterial efficacy of Sida acuta extracts against H. pylori.  …”
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Establishment and application of a rapid new detection method for antimicrobial susceptibility testing of Klebsiella pneumoniae based on MALDI-TOF MS by Yapei Zhang, Fanghua Fan, Xuan Wang, Jie Zhu, Shilei Dong

“…Our objective was to establish a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based antimicrobial susceptibility test (AST) for K. pneumoniae that produces reliable results within a few hours. …”
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Systemic delivery of AAV5, AAV8, and AAV9 packaging a C5-12-microdystrophin-FLAG expression cassette in non-human primates by Mengping Liu, Erica Cook, Yanshan Dai, Erich Ehlert, Francois du Plessis, Jacek Lubelski, Bogdan G. Sleczka, Petia Shipkova, Zhuyin Li, Joshua Gamse, David Gordon, Leonard P. Adam, Paul C. Levesque, Glen B. Banks

“…At 1 month post-dose, we compared tissue vector genomes, mRNA, and microdystrophin-FLAG protein levels by meso-scale discovery-enzyme-linked immunosorbent assay, liquid chromatography-mass spectrometry, and immunofluorescence. The C5-12 promoter was highly selective for heart and skeletal muscles, when compared to off-target tissues such as peripheral blood mononuclear cells, lung, liver, and kidney. …”
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