Application of urinary peptide-biomarkers in trauma patients as a predictive tool for prognostic assessment, treatment and intervention timing by Gökmen Aktas, Felix Keller, Justyna Siwy, Agnieszka Latosinska, Harald Mischak, Jorge Mayor, Jan Clausen, Michaela Wilhelmi, Vesta Brauckmann, Stephan Sehmisch, Tarek Omar Pacha

“…To test the hypothesis that peptide biomarkers could predict patient outcomes in severely injured patients, we initiated a pilot study involving consecutive urine sampling (on days 0, 2, 5, 10, and 14) and subsequent peptidome analysis using capillary electrophoresis coupled to mass spectrometry (CE-MS) of 14 severely injured patients and two additional intensive care unit patients. …”
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Distribution and Antifungal Susceptibility Profile of Oropharyngeal Candida Species Isolated from People Living with HIV in the Era of Universal Test and Treat Policy in Uganda. by Musinguzi, Benson, Turyamuhika, Laban, Mwesigwa, Alex, Nalumaga, Pauline Petra, Kabajulizi, Immaculate, Njovu, Israel Kiiza, Mwebesa, Edson, Luggya, Tonny, Ocheng, Francis, Kateete, David Patrick, Itabangi, Herbert, Mboowa, Gerald, Obondo, James Sande, Achan, Beatrice

“…Oropharyngeal swabs were collected and cultured on Sabouraud Dextrose agar to isolate Candida species identified using the matrix-assisted laser desorption ionization-time of flight mass spectrometry. Additionally, the antifungal susceptibility profile of Candida isolates to six antifungal drugs was determined using the VITEK® (Marcy-l’Étoile, France) compact system. …”
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SNORA37/CMTR1/ELAVL1 feedback loop drives gastric cancer progression via facilitating CD44 alternative splicing by Banghe Bao, Minxiu Tian, Xiaojing Wang, Chunhui Yang, Jiaying Qu, Shunchen Zhou, Yang Cheng, Qiangsong Tong, Liduan Zheng

“…Biotin-labeled RNA pull-down, mass spectrometry, cross-linking RNA immunoprecipitation, and in vitro binding assays were applied to explore interaction of snoRNAs with protein partners. …”
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Effects of ultra-processed foods on the liver: insights from gut microbiome and metabolomics studies in rats by Liping Shi, Zhuoyuan Li, Xiaojun Ma, Junru Wang, Yueping Wu, Yongbin Zhu, Yanrong Wang, Yue Yang, Minxiu Luo, Jiangping Li, Xian Sun, Xian Sun, Shulan He, Shulan He

“…Some serum biochemical indices, inflammatory factors, oxidative stress markers, hematoxylin–eosin (HE) staining of the liver, 16S ribosomal RNA (rRNA) and Liquid chromatography-mass spectrometry (LC–MS) of rat feces were detected.ResultsThe UPF diet-induced simple steatosis of the liver in rats without affecting the levels of IL-6, GSH, MDA, and SOD. …”
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Dietary phytochemicals alleviate the premature skin aging: A comprehensive review by Harpreet Singh, Y.T. Kamal, Jessica Pandohee, Arun Kumar Mishra, Aritra Biswas, Sourav Mohanto, Arvind Kumar, Sagnik Nag, Amrita Mishra, Mhaveer Singh, Himanshu Gupta, Hitesh Chopra

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A preliminary study on the reference intervals of vitamin K in some areas of Beijing with normal physical examination population by Lin Chen, Meiqi Chen, Shi Cheng, Jiaxin Fei, Dan Xu, Xueyun Hou, Nannan Li, Yuliang Yuan, Guijian Liu, Cheng An

“…Serum VK1, MK4, and MK7 were firstly measured by high-performance liquid chromatography and mass spectrometry in 434 subjects. The reference intervals for three indicators were established by calculating the data of 2.5 and 97.5 percentiles. …”
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Endoscopic surgery affects the gut microbiota and its metabolism in breast cancer patients by Jingtai Li, Fangfang Gao, Runwei Li, Zhilin Chen, Guoping Chen, Pingming Fan, Guankui Du, Guankui Du, Guankui Du

“…The gut microbiota composition was determined using 16S rRNA sequencing, while the metabolites were analyzed through liquid chromatography-tandem mass spectrometry (LC-MS/MS). Bioinformatics and statistical analyses were employed to identify significant alterations in microbial taxa abundance and to assess intergroup differences. …”
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Blanching-Induced Changes in Polyphenol Oxidase, Antioxidants and Phenolic Profile of Mangosteen Pericarp by Giroon Ijod, Nur Izzati Mohamed Nawawi, Rabiha Sulaiman, Noranizan Mohd Adzahan, Farooq Anwar, Ezzat Mohamad Azman

“…Additionally, the phenolic compounds were identified using liquid chromatography-mass spectrometry (LC-MS). Results and conclusions. Zero-order reaction kinetics (R2>0.800) showed that residual PPO activity was significantly (p<0.05) reduced in both blanched and steamed mangosteen pericarp. …”
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Residual Characteristics and Dietary Risk Assessment of Dinotefuran and Tolfenpyrad in Tea Processing by LI Chunyong, LÜ Ying, JIN Jing, WANG Xia, QIN Shu

“…The samples were extracted using acetonitrile containing 5% formic acid, salted out, purified by dispersed solid phase extraction, detected by ultra-high performance liquid chromatography-tandem mass spectrometry, and quantified by an external standard method. …”
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Screening Southeast Asian medicinal plants: Clausena lansium, Leea indica, Strobilanthes crispa, Vitex trifolia for anti-platelet aggregation and blood coagulation effects by Sogand Zareisedehizadeh, Fu-Xun Heng, Chay-Hoon Tan, Paul Chi-Lui Ho, Hwee-Ling Koh

“…Liquid chromatography and time of flight mass spectrometry (LC-ToF-MS) analysis of Leea indica revealed gallic acid, methyl gallate, epigallocatechin, epigallocatechin-3-O-gallate, and other polyphenolic compounds. …”
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Effect of SNORD113-3/ADAR2 on glycolipid metabolism in glioblastoma via A-to-I editing of PHKA2 by Zheng Cui, Xiaobai Liu, Tiange E, Hongda Lin, Di Wang, Yunhui Liu, Xuelei Ruan, Ping Wang, Libo Liu, Yixue Xue

“…ADAR2 mediates the A-to-I RNA editing of PHKA2 mRNA. Mass spectrometry analysis and in vitro kinase testing revealed that PHKA2 phosphorylates EBF1 on Y256, reducing the stability and expression of EBF1. …”
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A sterol panel for rare lipid disorders: sitosterolemia, cerebrotendinous xanthomatosis and Smith-Lemli-Opitz syndrome by Alexander Bauer Westbye, Lili L. Dizdarevic, Sandra R. Dahl, Emil Andreas Asprusten, Yngve Thomas Bliksrud, Anita Lövgren Sandblom, Ulf Diczfalusy, Per M. Thorsby, Kjetil Retterstøl

“…A simple and rapid method to extract free sterols from human serum and quantitate their concentration using isotope-dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) without derivatization was developed. …”
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Gas-phase fractionation DDA promotes in-depth DIA phosphoproteome analysis by Zhiwei Tu, Yabin Li, Shuhui Ji, Shanshan Wang, Rui Zhou, Gertjan Kramer, Yu Cui, Fei Xie

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Targeted protein degradation through site-specific antibody conjugation with mannose 6-phosphate glycan by Kaori Mukai, Robert Cost, Xin Sheen Zhang, Emily Condiff, Joanne Cotton, Xiaohua Liu, Ekaterina Boudanova, Björn Niebel, Peter Piepenhagen, Xinming Cai, Anna Park, Qun Zhou

“…The conjugate is mainly homogeneous by analysis using mass spectrometry, typically with one or two glycans coupled. …”
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Deciphering the pseudouridine nucleobase modification in human diseases: From molecular mechanisms to clinical perspectives by Shiheng Jia, Xue Yu, Na Deng, Chen Zheng, Mingguang Ju, Fanglin Wang, Yixiao Zhang, Ziming Gao, Yanshu Li, Heng Zhou, Kai Li

“…Highlights Methods to detect pseudouridine were introduced from classic mass spectrometry‐based methods to newer approaches such as nanopore‐based technologies and BID sequencing, each with its advantages and limitations. …”
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Prevalence of Vitamin K2 Deficiency and Its Association with Coronary Artery Disease: A Case–Control Study by Sameh A. Ahmed, Abdulaziz A. Yar, Anas M. Ghaith, Rayan N. Alahmadi, Faisal A. Almaleki, Hassan S. Alahmadi, Waleed H. Almaramhy, Ahmed M. Alsaedi, Man K. Alraddadi, Hussein M. Ismail

“…Methods: This case–control study enrolled CAD patients and healthy controls to assess and compare serum concentrations of two vitamin K2 analogs including menaquinone-4 (MK-4) and menaquinone-7 (MK-7) via ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). CAD risk factors were evaluated and related to serum levels of vitamin K2 analogs. …”
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Nivolumab plasma concentration and clearance associated with overall survival in patients with renal cell carcinoma by Aurélien Marabelle, Laurence Albiges, Angelo Paci, Cécile Dalban, David Combarel, Sophie Broutin, Christophe Maritaz, Louis Blondel

“…Applying the model to a patient population with renal cell carcinoma identified nivolumab clearance and plasma concentration as predictors of overall survival (OS).Methods A custom liquid chromatography with tandem mass spectrometry method for quantifying nivolumab plasma concentration was developed and validated following the European Medicines Agency guidelines for bioanalytical method validation. …”
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Ion suppression correction and normalization for non-targeted metabolomics by Iqbal Mahmud, Bo Wei, Lucas Veillon, Lin Tan, Sara Martinez, Bao Tran, Alexander Raskind, Felice de Jong, Yiwei Liu, Jibin Ding, Yun Xiong, Wai-kin Chan, Rehan Akbani, John N. Weinstein, Chris Beecher, Philip L. Lorenzi

“…Abstract Ion suppression is a major problem in mass spectrometry (MS)-based metabolomics; it can dramatically decrease measurement accuracy, precision, and sensitivity. …”
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Removal of Matrix Interferences by Nano-MgO and Co-Adsorbents for Accurate Multi-Pesticide Residue Analysis in the Chinese Medicinal Herb, Paeoniae Radix Alba by Chunyu Wang, Xinquan Wang, Jiao Wang, Shanshan Di, Zhiwei Wang, Hao Xu, Huiyu Zhao, Changshan Zhao, Peipei Qi

“…Various PRA samples were extracted using 8 mL 0.5% acetic acid-acetonitrile solution and purified by a dispersive solid-phase extraction method with 30 mg nano-MgO, 40 mg primary secondary amine (PSA), and 40 mg octadecylsilane (C18) as the cleanup adsorbents, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). 70.7% of pesticides showed a weak matrix effect after the purification process, indicating that this method can give the precise quantitative analysis of trace pesticides residue. …”
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Association between gut microbiota and short-chain fatty acids in children with obesity by Shihan Li, Xinyu Ma, Hong Mei, Xuening Chang, Peiling He, Lingli Sun, Han Xiao, Shiqiong Wang, Ruizhen Li

“…Serum metabolite profiles were analyzed using high-performance liquid chromatography/mass spectrometry (LC/MS). The Statistical Package for the Social Sciences (SPSS, version 26) and R software were used for data analysis. …”
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