Assessment of Volatile Chemical Composition of the Essential Oil of Jatropha ribifolia (Pohl) Baill by HS-SPME-GC-MS Using Different Fibers by Celia Eliane de Lara da Silva, Willian Ferreira da Costa, Sandro Minguzzi, Rogério Cesar de Lara da Silva, Euclésio Simionatto

“…The Clevenger extractor was utilized in hydrodistillation of oil and chemical composition determined by gas chromatography coupled with mass spectrometry detector (GC-MS). The identification of compounds was confirmed by retention index (Kovats index) obtained from a series of straight chain alkanes (C7–C30) and by comparison with NIST and ADAMS library. …”
Get full text

Identification of nuclear valosin-containing-protein-like as a target of anti-nuclear autoantibodies in systemic sclerosis by Zitao Zeng, Ramona Miske, Madeleine Scharf, Yvonne Denno, Anthonina Ott, Stefanie Brakopp, Bianca Teegen, Winfried Stöcker, Elise Siegert, Elise Siegert, Sandra Saschenbrecker, Christian Probst, Lars Komorowski

“…Immunoprecipitation with fixed HEp-2 cells followed by SDS-PAGE and MALDI-TOF mass spectrometry was used to identify the autoantigen, which was verified by competitive inhibition experiments, recombinant HEK293 cell-based IFA, and Western and line blots based on the recombinant antigen. …”
Get full text

Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida by Rongchao He, Yanfei Zuo, Qiu Li, Qingpi Yan, Lixing Huang

“…We employed an array of methods including molecular dynamics simulations, molecular docking, ChIP-seq, RNA-seq, mass spectrometry, gene mutagenesis, LacZ reporter assays, electrophoretic mobility shift assays, co-immunoprecipitation, and in vitro LexA degradation experiments. …”
Get full text

Optimization of the "Loose Tea Blooming" Process for Guizhou Green Tea and Analysis of Characteristic Volatile Compounds by Linyao SONG, Hui XU, Siyao WU, Fengwei MA, Qiang FEI, Junyi DENG, Dajuan SHI, Yuze LIU, Yichen ZHAO, Xuan DONG, Su XU

“…Concurrently, volatile compounds in both the raw material and the final product were detected using headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS), followed by an analysis of the characteristic volatile compounds. …”
Get full text

A novel LC-MS/MS analysis of vitamin D metabolites in mice serum and hair: impact of diet and light exposure by Muhammad K. Hakeem, Asma Al-Menhali, Sampath K. Elangovan, Iltaf Shah

“…Serum and hair samples from mice were analyzed under various experimental conditions for vitamin D and its metabolites using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The experimental conditions included a vitamin D-deficient diet, a vitamin D-standard diet, and changes in ambient light exposure ranging from complete darkness to a regular light-dark cycle.ResultsMice fed a standard vitamin D diet and exposed to a regular light-dark cycle exhibited significantly higher levels of 25OHD3 in both serum and hair, indicating the synergistic effect of dietary vitamin D intake and light exposure. …”
Get full text

Ultrafast Sintering of Dense Li7La3Zr2O12 Membranes for Li Metal All‐Solid‐State Batteries by Faruk Okur, Huanyu Zhang, Julian F. Baumgärtner, Jaka Sivavec, Matthias Klimpel, Gregor Paul Wasser, Romain Dubey, Lars P.H. Jeurgens, Dmitry Chernyshov, Wouter vanBeek, Kostiantyn V. Kravchyk, Maksym V. Kovalenko

“…The chemical and structural evolution of LLZO membranes during the UFS process is characterized through in situ synchrotron X‐ray diffraction and thermogravimetric analysis‐mass spectrometry, complemented by an in‐depth investigation of surface chemistry using X‐ray photoelectron spectroscopy. …”
Get full text

Metabolites induced by citrus tristeza virus and ‘Candidatus Liberibacter asiaticus’ influence the feeding behavior of Diaphorina citri: an electrical penetration graph and LC–MS/M... by Jingtian Zhang, Fengchun Xia, Yuhe Li, Yangyang Liu, Fengnian Wu, Xiaoling Deng, Meirong Xu

“…Liquid chromatography with tandem mass spectrometry (LC–MS/MS) was applied to analyze the metabolites of the four groups of plants. …”
Get full text

Evaluation of Airborne Particulate Matter and Volatile Organic Compounds Released by Three Types of Mosquito Repellents by Soyeon Lee, Jongmin Park, Myoungho Lee, Minjung Kim, Boowook Kim, Kiyoung Lee, Kyungduk Zoh, Chungsik Yoon

“…PM was measured with a scanning mobility particle sizer (SMPS) and optical particle spectrometer (OPS) in an exposure chamber; VOCs were collected with Tenax TA and analyzed by gas chromatography-mass spectrometry. SMPS measurements in the range of 10–420 nm showed that the coil-types had the highest concentration (13,048 particles cm‒3), followed by the mat-types (119 particles cm‒3), and liquid-types (42 particles cm‒3). …”
Get full text

Dynamically Cyclic Fe2+/Fe3+ Active Sites as Electron and Proton-Feeding Centers Boosting CO2 Photoreduction Powered by Benzyl Alcohol Oxidation by Ben Lei, Gaofeng Zhou, Zhongyou Gong, Chao Liu, Ying Zhou, Vitaliy P. Guro, Yanjuan Sun, Jianping Sheng, Fan Dong

“…More importantly, in situ attenuated total reflection Fourier transform infrared spectroscopy and gas chromatography–mass spectrometry spectroscopy, with isotope labeling of Deuteron-benzyl alcohol and 13CO2, confirmed that proton transfer from the hydroxyl group generates reactive protons at the Fe2+/Fe3+ site, enabling efficient CO2 photoreduction through subsequent protonation steps. …”
Get full text

RETRACTED: Melanin Produced by Bordetella parapertussis Confers a Survival Advantage to the Bacterium during Host Infection by Yukihiro Hiramatsu, Takashi Nishida, Dendi Krisna Nugraha, Fuminori Sugihara, Yasuhiko Horiguchi

“…Our results also indicate that the brown pigment produced by the bacterium is melanin, because HppD is involved in the biosynthesis of a type of melanin called pyomelanin, and homogentisic acid, the monomeric precursor of pyomelanin, was detected by high-performance liquid chromatography-mass spectrometry analyses. In an infection assay using macrophages, the hppD-deficient mutant was internalized by THP-1 macrophage-like cells, similar to the wild-type strain, but was less able to survive within the cells, indicating that melanin protects B. parapertussis from intracellular killing in macrophages. …”
Get full text

Perspectives of proteomic analysis in ophthalmology by A.A. Krylova, A.V. Zakharchuk, O.I. Krivosheina, S.I. Pekov, I.A. Popov

“…</p> <p> <b>Keywords: </b>proteomic analysis, tear, aqueous humor, mass spectrometry, eye disorders<b>.</b> </p> <p> <b>For citation:</b> Krylova A.A., Zakharchuk A.V., Krivosheina O.I., Pekov S.I., Popov I.A. …”
Get full text

Pharmacokinetics and Bioequivalence of Two Fixed-Dose Combination Tablets of Valsartan/Amlodipine (80/5 Mg) in Healthy Chinese Subjects by Tian M, Huang J, Chen Y, Jin Q, Jiang H, Shi C, Mei J, Xu M, Yu X, Yang S

“…Plasma concentrations of valsartan and amlodipine were determined using ultrahigh-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS), and the noncompartmental analysis method was used for estimating the pharmacokinetic parameters.Results: Under fasting conditions, the within-subject standard deviations (Swr) of maximum plasma concentration (Cmax), area under the concentration–time curve from time 0 to the time of the last-measurable plasma concentration (AUC0−t), and area under the concentration–time curve from time 0 extrapolated to infinity (AUC0−∞) for valsartan were calculated to be ≥ 0.294 and evaluated by the reference-scaled average bioequivalence (RSABE) method. …”
Get full text

Microbial phenolic metabolites are associated with better frontal lobe cognition by Inés Domínguez-López, Isabella Parilli-Moser, Anna Vallverdú-Queralt, Anna Tresserra-Rimbau, Cinta Valls-Pedret, Zenaida Vázquez-Ruiz, Olga Castañer, Ramon Estruch, Emili Ros, Rosa M. Lamuela-Raventós

“…A novel method based on liquid chromatography coupled to mass spectrometry was used to identify urinary MPM (protocatechuic acid, enterodiol glucuronide, enterolactone glucuronide, urolithin B glucuronide, and vanillic acid glucuronide), and cognitive function was evaluated with neuropsychological tests. …”
Get full text

Analysis of thyroid function and structure and serum metabolomics in pregnant rats exposed to airborne contaminants: Combined perchlorate, thiocyanate, and nitrate exposure by Chenchen Wang, Xiaohui Xu, Jiangling Yuan, Ran An, Aierken Taxitiemuer, Yan Zhang, Tao Luo, Jing Zhou, Chang Wang, Ling Zhang, Shunhua Wu

“…We used liquid chromatography-tandem mass spectrometry metabolomics methods to investigate corresponding changes in serum metabolites and metabolic biomarkers and identify metabolism-related pathways. …”
Get full text

Biocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce Drop by Lihui Xu, Qinghua Shang, Mogens Nicolaisen, Rong Zeng, Shigang Gao, Ping Gao, Zhiwei Song, Fuming Dai, Jingze Zhang

“…Lipopeptide compounds produced by each strain were identified using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI–TOF MS) analysis. Both strains produced three types of lipopeptides, namely surfactins, iturins, and fengycins, whereas Bac20 showed the strongest intensity in its production of iturins, more than that of Bac45. …”
Get full text

Age but not vitamin D is related to sarcopenia in vitamin D sufficient male elderly in rural China by Weinuo Mi, Huifeng Zhang, Lina Zhang, Xingjia Li, Zhiguo Wang, Yu Sun, Tonggao Shen, Kuanlu Fan, Chao Liu, Shuhang Xu

“…Serum 25(OH)D levels were measured using the liquid chromatography with tandem mass spectrometry. Correlations of serum 25(OH)D level with sarcopenia and its components in Chinese elderly were identified by the binary logistic regression and linear regression analyses. …”
Get full text

Rapid Screening and Quantitative Determination of Illegal Phosphodiesterase Type 5 Inhibitors (PDE-5i) in Herbal Dietary Supplements by Thi Oanh Nguyen, Cao-Son Tran, Thi Thu Hang Do, Thi Minh Hoa Nguyen, Quang-Dong Bui, Cao-Tien Bui, Hong-Ngoc Nguyen, Thu-Hien Dang, Viet-Chien Dinh, Thi Anh Huong Nguyen, Thi Hong Hao Le

“…Liquid chromatography coupled with high-resolution mass spectrometry has become one of the most effective methods to identify and measure PDE-5i concentration. …”
Get full text

Phosphorylation of Bok at Ser-8 blocks its ability to suppress IP3R-mediated calcium mobilization by Caden G. Bonzerato, Katherine R. Keller, Richard J. H. Wojcikiewicz

“…Methods Bok Ser-8 phosphorylation was characterized using purified proteins, G-protein coupled receptor agonists that increase cAMP levels in intact cells, mass spectrometry, and immunoreactivity changes. Also, using mammalian cells that exclusively or predominately express IP3R1, to which Bok binds strongly, and a fluorescent Ca2+-sensitive dye or a genetically-encoded Ca2+ sensor, we explored how endogenous and exogenous Bok controls the Ca2+ mobilizing function of IP3R1, and whether Bok phosphorylation at Ser-8, or replacement of Ser-8 with a phosphomimetic amino acid, is regulatory. …”
Get full text

Valorization of local regional apple (Malus domestica Borkh.) cultivars versus commercial samples from Spain: Phenolic compounds by HPLC-MS/MS, cytotoxicity and biological potentia... by Adrián Millán-Laleona, Pilar Cebollada, Giovanni Caprioli, Diletta Piatti, Filippo Maggi, Ana Pina, Carlota Gómez-Rincón, Víctor López

“…The phenolic profile of the regional and commercial Spanish apple cultivars was performed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). For a deeper understanding of their anti-inflammatory and antioxidant potential, the 5-lipoxigenase inhibition and NO− scavenging were evaluated. …”
Get full text

Engineering p‐Orbital States via Molecular Modules in All‐Organic Electrocatalysts toward Direct Water Oxidation by Li‐Hong Yu, Xue‐Feng Zhang, Zi‐Ming Ye, Hong‐Gang Du, Li‐Dong Wang, Ping‐Ping Xu, Yuhai Dou, Li‐Ming Cao, Chun‐Ting He

“…Operando differential electrochemical mass spectrometry (DEMS) with isotope labeling identifies the adsorbate evolution mechanism (AEM). …”
Get full text