Comprehensive characterization of higher order structure changes in methionine oxidized monoclonal antibodies via NMR chemometric analysis and biophysical approaches by Mingyue Li, Victor A. Beaumont, Shahajahan Akbar, Hannah Duncan, Arch Creasy, Wenge Wang, Kelly Sackett, Lisa Marzilli, Jason C. Rouse, Hai-Young Kim

“…We used a forced degradation method involving concentration-dependent oxidation by peracetic acid, in which Met oxidation is site-specifically quantified by liquid chromatography-mass spectrometry. Conventional biophysical techniques report nuanced results, in which CD detects no change to the secondary structure and little change in the tertiary structure. …”
Get full text

Entomological assessment of hessian fabric transfluthrin vapour emanators for protecting against outdoor-biting Aedes aegypti in coastal Tanzania. by Nicodem J Govella, Alphonce Assenga, Amos T Mlwale, Nosrat Mirzai, Eimear Heffernan, Jennie Moriarty, John Wenger, Vincent Corbel, Justin McBeath, Sheila B Ogoma, Gerry F Killeen

“…Preliminary measurements of transfluthrin vapour concentration in air samples collected near treated emanators were conducted by gas chromatography-mass spectrometry.<h4>Results</h4>Two full field experiments with four different emanator designs and three different transfluthrin formulations consistently indicated negligible reduction of human landing rates by wild Ae. aegypti. …”
Get full text

Elevated fasting serum xylitol levels are associated with a lower risk of incident type 2 diabetes among individuals with prediabetes in the Chinese population by Yicheng Qi, Xiaoyong Li, Yu Liu, Meng Wang, Yiming Wu, Qing Su, Li Qin, Jing Ma

“…The baseline levels of serum polyols in the fasting state were quantified using time-of-flight mass spectrometry. Multivariate conditional logistic regression was performed to assess the effects of the differential polyol levels on the risk of incident diabetes from prediabetes. …”
Get full text

Utility of Integrated Analysis of Pharmacogenomics and Pharmacometabolomics in Early Phase Clinical Trial: A Case Study of a New Molecular Entity by Jaeseong Oh, Sojeong Yi, Namyi Gu, Dongseong Shin, Kyung-Sang Yu, Seo Hyun Yoon, Joo-Youn Cho, In-Jin Jang

“…Metabolomics profiling was performed from plasma samples using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. A GSTM1 null polymorphism was identified in pharmacogenomics test and the drug concentrations was higher in GSTM1 null subjects than GSTM1 functional subjects. …”
Get full text

Model Analytical Development for Physical, Chemical, and Biological Characterization of Momordica charantia Vegetable Drug by Deysiane Oliveira Brandão, Geovani Pereira Guimarães, Ravely Lucena Santos, Fernando José de Lima Ramos Júnior, Karla Monik Alves da Silva, Fabio Santos de Souza, Rui Oliveira Macêdo

“…The herbal medicine raw material derived from the leaves and fruits in different particle sizes was analyzed using thermoanalytical techniques as thermogravimetry (TG) and differential thermal analysis (DTA), pyrolysis coupled to gas chromatography/mass spectrometry (PYR-GC/MS), and nuclear magnetic resonance (1H NMR), in addition to the determination of antimicrobial activity. …”
Get full text

Identification of ketamine and norketamine in dried bloodstains on crime-scene surfaces by Risha Jasmine Nathan, Babajide Okeleye, Rabiu Abdullahi, Waliyu Oyebode

“…Analytes were solvent-extracted using a dichloromethane: hexane (1:3 v/v) mixture, followed by gas chromatography-mass spectrometry (GC–MS) analysis with ketamine-d4 as the internal standard. …”
Get full text

Investigating the Allelopathic and Bioherbicidal Potential of <i>Solidago altissima</i> with a Focus on Chemical Signaling in <i>Trifolium repens</i> by Ho-Jun Gam, Arjun Adhikari, Yosep Kang, Md. Injamum-Ul-Hoque, Shifa Shaffique, Ji-In Woo, Jin Ryeol Jeon, Byeong-Kwan An, Min Young Back, Ki-Yong Kim, Sang-Mo Kang, In-Jung Lee

“…Gas chromatography-mass spectrometry analysis of <i>S. altissima</i> root extract identified a high concentration of methyl kolavenate, a clerodane diterpene predicted to act as a phytotoxic agent. …”
Get full text

Aroma profiling of long shelf-life yoghurt from milk with varying acidity: Insights from sensory evaluation and instrumental analysis by Jia Wang, Dongjie Zhang, Jie Li, Tingting Zou, Rong Wen, Yaqiong Hou, Huanlu Song, Hongliang Li, Hairan Ma, Jiao Shi

“…In this study, the aroma components of long shelf-life yoghurt produced from milk with varying acidity levels were identified using the Stir Bar Sorptive Extraction (SBSE) coupled with comprehensive two-dimensional gas chromatography−olfactometry−mass spectrometry technology (GC × GC-O-MS) for the first time. …”
Get full text

Simultaneous determination of nirmatrelvir, ritonavir, and beta-D-N4-hydroxycytidine in human plasma and epithelial lining fluid using LC-MS/MS and its clinical application to comp... by Wenjing Zhang, Lin Xia, Zhilong Yuan, Mengdan Liu, Yang Jiao, Zhuo Wang

“…The analysis method was developed and validated to simultaneously quantify nirmatrelvir, ritonavir, and beta-D-N4-hydroxycytidine (NHC) in human plasma and bronchoalveolar lavage fluid (BALF) by electrospray ionization mass spectrometry.Our method was validated over a linear range of 30–10000 ng/mL for both matrices, with precision and accuracy within 15 % across four concentrations. …”
Get full text

Glutamine missense suppressor transfer RNAs inhibit polyglutamine aggregation by Rasangi Tennakoon, Teija M.I. Bily, Farah Hasan, Sunidhi Syal, Aaron Voigt, Tugce B. Balci, Kyle S. Hoffman, Patrick O’Donoghue

“…In contrast, cells expressing tRNAAlaCUG selectively decreased insoluble polyQ aggregate formation by 2-fold. Mass spectrometry confirmed Ala mis-incorporation at an average level of ∼20% per Gln codon. …”
Get full text

Bioprocess exploitation of microaerobic auto-induction using the example of rhamnolipid biosynthesis in Pseudomonas putida KT2440 by Jakob Grether, Holger Dittmann, Leon Willems, Tabea Schmiegelt, Elvio Henrique Benatto Perino, Philipp Hubel, Lars Lilge, Rudolf Hausmann

Get full text

Mapping Nanoscale‐To‐Single‐Cell Phosphoproteomic Landscape by Chip‐DIA by Gul Muneer, Sofani Tafesse Gebreyesus, Ciao‐Syuan Chen, Tzu‐Tsung Lee, Fengchao Yu, Chih‐An Lin, Min‐Shu Hsieh, Alexey I. Nesvizhskii, Chao‐Chi Ho, Sung‐Liang Yu, Hsiung‐Lin Tu, Yu‐Ju Chen

“…Here, we present a Chip‐DIA strategy, integrating a microfluidic‐based phosphoproteomic chip (iPhosChip) with data‐independent acquisition mass spectrometry (DIA‐MS) for ultrasensitive nanoscale‐to‐single‐cell phosphoproteomic profiling. …”
Get full text

Data on compositional diversity of textural varieties of lake superior-type Banded Iron Formation (BIF) of the Njweng prospect, Mbalam iron ore district, southern CameroonMendeley... by Dieudonne Charles Isidore Ilouga, Cheo Emmanuel Suh, Akumbom Vishiti, Elisha Muntum Shemang

“…Chemical analysis was accomplished using a combination of the Inductively Coupled Plasma Emission Spectrometry (ICP-ES) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) on the major and trace elements respectively. …”
Get full text

Investigation of newborn blood metabolomics in varying intrauterine growth conditions by Shengwen Wang, Xiaofei Lin, Yu Zhou, Xin Yang, Mingming Ou, Linxin Zhang, Yumei Wang, Jing Gao

“…Blood samples were obtained from all newborns between 3 and 5 days after birth and analyzed using tandem mass spectrometry to detect blood metabolites. Subsequently, we screened for different metabolites and metabolic pathways among the groups using the MetaboAnalystR package (Version 1.0.1) in R software (R-3.6.0). …”
Get full text

Monitoring of the Gasoline Oxygenate MTBE and BTEX Compounds in Groundwater in Catalonia (Northeast Spain) by J. Fraile, J.M. Niaerola, L. Olivella, M. Figueras, A. Ginebreda, M. Vilanova, D. Barcela

“…Headspace (HS) gas chromatography with flame ionisation detection (HS-GC-FID) and purge and trap (P) gas chromatography-mass spectrometry (P) were used for the determination of methyl-tert-butyl ether (MTBE) and benzene, toluene, and xylenes (BTEX) in groundwater. …”
Get full text

Association of ATM Gene Polymorphism with PTC Metastasis in Female Patients by Yulu Gu, Xiaoli Liu, Yaqin Yu, Jieping Shi, Lizhe Ai, Hui Sun, Joseph Sam Kanu, Chong Wang, Yawen Liu

“…Four ATM single nucleotide polymorphisms (SNPs) were genotyped using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS). Association between genotypes and the gender-specific risk of metastasis was assessed by odds ratios (OR) and 95% confidence intervals (CI) under the unconditional logistic regression analysis. …”
Get full text

UPLC-MS/MS Method for Givinostat in Rat Plasma: Development, Validation, in vivo Pharmacokinetics Study and in vitro Metabolic Stability Research by Zhan R, Liu Y, Wu J, Shen Y, Xu X, Lin G, Chen X

“…Ruanjuan Zhan,1,&ast; Yanan Liu,1,2,&ast; Jun Wu,1,2 Yuxin Shen,1,2 Xinhao Xu,1,2 Guanyang Lin,1,2 Xiaocheng Chen1 1The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, People’s Republic of China; 2School of Pharmaceutical Sciences, Wenzhou Medical University, Zhejiang, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Guanyang Lin; Xiaocheng Chen, Email 13867702133@163.com; cxcwzcn@139.comBackground: Givinostat, a potent histone deacetylase (HDAC) inhibitor, is promising for the treatment of relapsed leukemia and myeloma.Purpose: This study aimed to develop and verify a quick assay for the measurement of givinostat concentration using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) with eliglustat as the internal standard (IS), establishing a basic pharmacokinetic profile for its pre-clinical application and metabolic stability in vitro.Methods: Sample preparation was performed via protein precipitation using acetonitrile. …”
Get full text

Synovial Fluid Cell Proteomic Analysis Identifies Upregulation of Alpha-Taxilin Proteins in Rheumatoid Arthritis: A Potential Prognostic Marker by Ashish Sarkar, Shivani Sharma, Prachi Agnihotri, Tanmoy Sarkar, Pooja Kumari, Rajesh Malhotra, Barun Datta, Vijay Kumar, Sagarika Biswas

“…Two-dimensional gel electrophoresis (2-DE) was performed using synovial fluid cells of RA and osteoarthritis (OA) patients, and 7 differentially expressed proteins were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS/MS). Αlpha-Taxilin (α-Taxilin) has been found as one of the novel, significantly up regulated protein in RA. …”
Get full text

Standard operating procedure combined with comprehensive quality control system for multiple LC-MS platforms urinary proteomics by Xiang Liu, Haidan Sun, Xinhang Hou, Jiameng Sun, Min Tang, Yong-Biao Zhang, Yongqian Zhang, Wei Sun, Chao Liu, Urine Test Sample Working Group

“…At present, the comparability of urinary proteomics data across diverse liquid chromatography−mass spectrometry (LC-MS) platforms remains an area that requires investigation. …”
Get full text

Tear Film Proteome of Healthy Domestic Cats by Jéssica Fontes Veloso, Paula Elisa Brandão Guedes, Luciana Carvalho Lacerda, Juliano Oliveira Santana, Irma Yuliana Mora-Ocampo, Carlos Priminho Pirovani, Arianne Pontes Oriá, Alexandre Dias Munhoz, Renata Santiago Alberto Carlos

“…Schirmer tear test strips were used to collect tear samples of twelve healthy cats, which were mixed, centrifuged, and placed in a single 1.5 mL microtube that was frozen at −20°C, until analysis by two-dimensional polyacrylamide gel and mass spectrometry associated with high-performance liquid chromatography. …”
Get full text