-
1221
Recent advances in detection techniques for vitamin analysis: A comprehensive review
Published 2025-02-01“…Here, we propose a comprehensive and critical evaluation of detection methods for water- and fat-soluble vitamins that have been studied over the past five years, including microbiology-, spectroscopy-, liquid chromatography–mass spectrometry-, electrochemistry-, sensor-, and immunoassay-based analysis techniques. …”
Get full text
Article -
1222
Full-length and N-terminally truncated recombinant interleukin-38 variants are similarly inefficient in antagonizing interleukin-36 and interleukin-1 receptors
Published 2025-01-01“…Methods The N-terminus of the IL-38 protein produced in a human keratinocyte cell line and of endogenous epidermal IL-38 isolated from healthy human skin was characterized by mass spectrometry. The effects of various recombinant forms of IL-38 on IL-36R- and IL-1R1-mediated responses were assessed in IL-36R HEK Blue reporter cells and in a normal human keratinocyte cell line. …”
Get full text
Article -
1223
Assessment of Volatile Chemical Composition of the Essential Oil of Jatropha ribifolia (Pohl) Baill by HS-SPME-GC-MS Using Different Fibers
Published 2013-01-01“…The Clevenger extractor was utilized in hydrodistillation of oil and chemical composition determined by gas chromatography coupled with mass spectrometry detector (GC-MS). The identification of compounds was confirmed by retention index (Kovats index) obtained from a series of straight chain alkanes (C7–C30) and by comparison with NIST and ADAMS library. …”
Get full text
Article -
1224
Identification of nuclear valosin-containing-protein-like as a target of anti-nuclear autoantibodies in systemic sclerosis
Published 2025-01-01“…Immunoprecipitation with fixed HEp-2 cells followed by SDS-PAGE and MALDI-TOF mass spectrometry was used to identify the autoantigen, which was verified by competitive inhibition experiments, recombinant HEK293 cell-based IFA, and Western and line blots based on the recombinant antigen. …”
Get full text
Article -
1225
Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida
Published 2025-01-01“…We employed an array of methods including molecular dynamics simulations, molecular docking, ChIP-seq, RNA-seq, mass spectrometry, gene mutagenesis, LacZ reporter assays, electrophoretic mobility shift assays, co-immunoprecipitation, and in vitro LexA degradation experiments. …”
Get full text
Article -
1226
A novel LC-MS/MS analysis of vitamin D metabolites in mice serum and hair: impact of diet and light exposure
Published 2025-02-01“…Serum and hair samples from mice were analyzed under various experimental conditions for vitamin D and its metabolites using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The experimental conditions included a vitamin D-deficient diet, a vitamin D-standard diet, and changes in ambient light exposure ranging from complete darkness to a regular light-dark cycle.ResultsMice fed a standard vitamin D diet and exposed to a regular light-dark cycle exhibited significantly higher levels of 25OHD3 in both serum and hair, indicating the synergistic effect of dietary vitamin D intake and light exposure. …”
Get full text
Article -
1227
Metabolites induced by citrus tristeza virus and ‘Candidatus Liberibacter asiaticus’ influence the feeding behavior of Diaphorina citri: an electrical penetration graph and LC–MS/M...
Published 2025-02-01“…Liquid chromatography with tandem mass spectrometry (LC–MS/MS) was applied to analyze the metabolites of the four groups of plants. …”
Get full text
Article -
1228
Evaluation of Airborne Particulate Matter and Volatile Organic Compounds Released by Three Types of Mosquito Repellents
Published 2024-03-01“…PM was measured with a scanning mobility particle sizer (SMPS) and optical particle spectrometer (OPS) in an exposure chamber; VOCs were collected with Tenax TA and analyzed by gas chromatography-mass spectrometry. SMPS measurements in the range of 10–420 nm showed that the coil-types had the highest concentration (13,048 particles cm‒3), followed by the mat-types (119 particles cm‒3), and liquid-types (42 particles cm‒3). …”
Get full text
Article -
1229
RETRACTED: Melanin Produced by Bordetella parapertussis Confers a Survival Advantage to the Bacterium during Host Infection
Published 2021-10-01“…Our results also indicate that the brown pigment produced by the bacterium is melanin, because HppD is involved in the biosynthesis of a type of melanin called pyomelanin, and homogentisic acid, the monomeric precursor of pyomelanin, was detected by high-performance liquid chromatography-mass spectrometry analyses. In an infection assay using macrophages, the hppD-deficient mutant was internalized by THP-1 macrophage-like cells, similar to the wild-type strain, but was less able to survive within the cells, indicating that melanin protects B. parapertussis from intracellular killing in macrophages. …”
Get full text
Article -
1230
Analysis of thyroid function and structure and serum metabolomics in pregnant rats exposed to airborne contaminants: Combined perchlorate, thiocyanate, and nitrate exposure
Published 2025-01-01“…We used liquid chromatography-tandem mass spectrometry metabolomics methods to investigate corresponding changes in serum metabolites and metabolic biomarkers and identify metabolism-related pathways. …”
Get full text
Article -
1231
Biocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce Drop
Published 2025-01-01“…Lipopeptide compounds produced by each strain were identified using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI–TOF MS) analysis. Both strains produced three types of lipopeptides, namely surfactins, iturins, and fengycins, whereas Bac20 showed the strongest intensity in its production of iturins, more than that of Bac45. …”
Get full text
Article -
1232
Rapid Screening and Quantitative Determination of Illegal Phosphodiesterase Type 5 Inhibitors (PDE-5i) in Herbal Dietary Supplements
Published 2021-01-01“…Liquid chromatography coupled with high-resolution mass spectrometry has become one of the most effective methods to identify and measure PDE-5i concentration. …”
Get full text
Article -
1233
Phosphorylation of Bok at Ser-8 blocks its ability to suppress IP3R-mediated calcium mobilization
Published 2025-01-01“…Methods Bok Ser-8 phosphorylation was characterized using purified proteins, G-protein coupled receptor agonists that increase cAMP levels in intact cells, mass spectrometry, and immunoreactivity changes. Also, using mammalian cells that exclusively or predominately express IP3R1, to which Bok binds strongly, and a fluorescent Ca2+-sensitive dye or a genetically-encoded Ca2+ sensor, we explored how endogenous and exogenous Bok controls the Ca2+ mobilizing function of IP3R1, and whether Bok phosphorylation at Ser-8, or replacement of Ser-8 with a phosphomimetic amino acid, is regulatory. …”
Get full text
Article -
1234
Engineering p‐Orbital States via Molecular Modules in All‐Organic Electrocatalysts toward Direct Water Oxidation
Published 2025-02-01“…Operando differential electrochemical mass spectrometry (DEMS) with isotope labeling identifies the adsorbate evolution mechanism (AEM). …”
Get full text
Article -
1235
Distribution and Antifungal Susceptibility Profile of Oropharyngeal Candida Species Isolated from People Living with HIV in the Era of Universal Test and Treat Policy in Uganda.
Published 2024“…Oropharyngeal swabs were collected and cultured on Sabouraud Dextrose agar to isolate Candida species identified using the matrix-assisted laser desorption ionization-time of flight mass spectrometry. Additionally, the antifungal susceptibility profile of Candida isolates to six antifungal drugs was determined using the VITEK® (Marcy-l’Étoile, France) compact system. …”
Get full text
Article -
1236
SNORA37/CMTR1/ELAVL1 feedback loop drives gastric cancer progression via facilitating CD44 alternative splicing
Published 2025-01-01“…Biotin-labeled RNA pull-down, mass spectrometry, cross-linking RNA immunoprecipitation, and in vitro binding assays were applied to explore interaction of snoRNAs with protein partners. …”
Get full text
Article -
1237
Effects of ultra-processed foods on the liver: insights from gut microbiome and metabolomics studies in rats
Published 2025-01-01“…Some serum biochemical indices, inflammatory factors, oxidative stress markers, hematoxylin–eosin (HE) staining of the liver, 16S ribosomal RNA (rRNA) and Liquid chromatography-mass spectrometry (LC–MS) of rat feces were detected.ResultsThe UPF diet-induced simple steatosis of the liver in rats without affecting the levels of IL-6, GSH, MDA, and SOD. …”
Get full text
Article -
1238
A preliminary study on the reference intervals of vitamin K in some areas of Beijing with normal physical examination population
Published 2025-01-01“…Serum VK1, MK4, and MK7 were firstly measured by high-performance liquid chromatography and mass spectrometry in 434 subjects. The reference intervals for three indicators were established by calculating the data of 2.5 and 97.5 percentiles. …”
Get full text
Article -
1239
Residual Characteristics and Dietary Risk Assessment of Dinotefuran and Tolfenpyrad in Tea Processing
Published 2025-01-01“…The samples were extracted using acetonitrile containing 5% formic acid, salted out, purified by dispersed solid phase extraction, detected by ultra-high performance liquid chromatography-tandem mass spectrometry, and quantified by an external standard method. …”
Get full text
Article -
1240
Screening Southeast Asian medicinal plants: Clausena lansium, Leea indica, Strobilanthes crispa, Vitex trifolia for anti-platelet aggregation and blood coagulation effects
Published 2025-02-01“…Liquid chromatography and time of flight mass spectrometry (LC-ToF-MS) analysis of Leea indica revealed gallic acid, methyl gallate, epigallocatechin, epigallocatechin-3-O-gallate, and other polyphenolic compounds. …”
Get full text
Article