Site-selective photo-crosslinking for the characterisation of transient ubiquitin-like protein-protein interactions. by Zac Sandy, Zijuan Wang, Deepak Behera, Benjamin M Foster, Finlay A Martin, Kira Brüninghoff, Kathleen M Cain, Wolfgang Dörner, Maria Jose Cabello-Lobato, Josep V Forment, Matthew J Cliff, Igor Larrosa, Perdita Barran, Duncan L Smith, Henning D Mootz, Christine K Schmidt

“…The stabilised protein complexes can be purified using affinity purification and identified by mass spectrometry. The resultant covalent bonds can withstand even the harshest washing conditions, allowing for the removal of indirect binders whilst retaining and capturing weak and transient interactors that are commonly lost during wash steps. …”
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Coloring hot-dip galvanization of steel samples in industrial zinc-manganese baths by Godzsák M., Lévai G., Vad K., Csik A., Hakl J., Kulcsár T., Kaptay G.

“…The results are analyzed by Glow-discharge optical emission spectroscopy (GD-OES) and Secondary Neutral Mass Spectrometry (SNMS) techniques. It is shown that depending on the Mn-content and on the wall thickness of the steel the samples are coated by MnO of various thicknesses (in the range between 30 - 230 nm). …”
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Investigating Metabolic Phenotypes for Sarcoidosis Diagnosis and Exploring Immunometabolic Profiles to Unravel Disease Mechanisms by Mohammad Mehdi Banoei, Abdulrazagh Hashemi Shahraki, Kayo Santos, Gregory Holt, Mehdi Mirsaeidi

“…<b>Methods:</b> Serum samples from patients with sarcoidosis (<i>n</i> = 40, including stage 1 to stage 4), were analyzed for metabolite levels by semi-untargeted liquid chromatography–mass spectrometry (LC-MS). Metabolomics data from patients with sarcoidosis were compared with those from patients with COVID-19 and healthy controls to identify distinguishing metabolic biosignatures. …”
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Energy Metabolism and Lipidome Are Highly Regulated during Osteogenic Differentiation of Dental Follicle Cells by Oliver Pieles, Marcus Höring, Sadiyeh Adel, Torsten E. Reichert, Gerhard Liebisch, Christian Morsczeck

“…Expression and activity of metabolic markers were evaluated by western blot analysis and specific assays, relative amount of mitochondrial DNA was measured by real-time quantitative polymerase chain reaction, the oxidative state of cells was determined by a glutathione assay, and the lipidome of cells was analyzed via mass spectrometry (MS). Moreover, osteogenic markers were analyzed after the inhibition of fatty acid synthesis by 5-(tetradecyloxy)-2-furoic acid or C75. …”
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A Critical Review of Proteomic Studies in Gestational Diabetes Mellitus by Tao Zhou, Lu Huang, Min Wang, Daozhen Chen, Zhong Chen, Shi-Wen Jiang

“…In the present review, we comprehensively summarized recent studies of gestational diabetes using mass spectrometry-based proteomic technologies. Focused on the entire experimental design and proteomic results, we showed that these studies have covered a broad range of research contents in terms of sampling time, sample types, and outcome associations. …”
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Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione by Nebojša Đ. Pantelić, Bojana B. Zmejkovski, Željko Žižak, Nebojša R. Banjac, Bojan Đ. Božić, Tatjana P. Stanojković, Goran N. Kaluđerović

“…A novel triphenyltin(IV) compound with 1-(4-carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione was synthesized and characterized by IR, NMR spectroscopy, mass spectrometry, and elemental analysis. In vitro anticancer activity of ligand precursor and synthesized organotin(IV) compound was determined against tumor cell lines: human adenocarcinoma (HeLa), human myelogenous leukemia (K562), and human breast cancer (MDA-MB-453), using microculture tetrazolium test (MTT) assay. …”
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Resistance of Biomphalaria alexandrina to Schistosoma mansoni and Bulinus truncatus to Schistosoma haematobium Correlates with Unsaturated Fatty Acid Levels in the Snail Soft Tissu... by Marian Elias, Rasha S. Hanafi, Samia El-Bardicy, Ebtisam A. Hafez, Rashika El Ridi

“…Snails were examined for presence or lack of infection association with soft tissue and hemolymph content of proteins, cholesterol, and triglycerides, evaluated using standard biochemical techniques and palmitic, oleic, linoleic, and arachidonic acid, assayed by ultraperformance liquid chromatography-tandem mass spectrometry. Successful schistosome infection of B. alexandrina and B. truncatus consistently and reproducibly correlated with snails showing highly significant (up to P<0.0001) decrease in soft tissue and hemolymph content of the monounsaturated fatty acid, oleic acid, and the polyunsaturated fatty acids, linoleic, and arachidonic acids as compared to naïve snails. …”
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Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp. bulgaricus in skimmed mil... by Yue Yang, Wenxia Zhou, Lingyu Yang, Yilun Chen, Dongxiao Sun-Waterhouse, Dapeng Li

“…This study aimed to help fill this knowledge gap and examined the degradation mechanism of organophosphorus pesticide, chlorpyrifos, in milk by Lactobacillus delbrueckii ssp. bulgaricus using gas chromatography-tandem mass spectrometry (GC-MS/MS) combined with transcriptome analysis. …”
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Simultaneous Quantitative and Qualitative Analysis of Flavonoids from Ultraviolet-B Radiation in Leaves and Roots of Scutellaria baicalensis Georgi Using LC-UV-ESI-Q/TOF/MS by Wen-Ting Tang, Min-Feng Fang, Xiao Liu, Ming Yue

“…Liquid chromatography-ultraviolet detection coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (LC-UV-ESI-Q/TOF/MS) method was applied for simultaneous quantitative and qualitative analysis of flavonoids in leaves and roots of S. baicalensis by enhanced UV-B radiation. …”
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Phytochemical Profiling, Bioactivity, and Insecticidal Effectiveness of <i>Mammea americana</i> L. Leaf Extracts Against <i>Ferrisia</i> sp. by Mike Vázquez-Torres, Nilka Rivera-Portalatín, Irma Cabrera-Asencio

“…Micro-Soxhlet extraction with chloroform, dichloromethane, and methanol was used, and key phytochemicals were identified via Gas Chromatography-Mass Spectrometry (GC-MS). The extracts were mainly composed of sesquiterpenes, carboxylic acids, coumarins, esters, diterpenes, and other bioactive compounds. …”
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Effect of Sun-exposure before Moisture-yellowing on the Aroma of Yellow Tea by Xinying CHEN, Junran QI, Lixia ZHANG

“…The tea samples were analyzed by the sensory evaluation and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) methods. The results showed that the optimal quality of yellow tea was obtained by sun-exposure to the leaf for 0.5 hours under the environmental conditions of light intensity 1700±50 μmol∙m−2∙s−1, outdoor temperature 35±5 ℃, and air humidity 30%±5%, and then moisture-yellowing for 3 hours at a temperature of 45 ℃ and humidity of 70%. …”
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Comparative proteomic analysis of astrocytoma tissues from patients with and without seizures by Thanakorn Khaosuwan, Kittinun Leetanaporn, Pongsakorn Choochuen, Raphatphorn Navakanitworakul, Anukoon Kaewborisutsakul, Thara Tunthanatip, Surasak Sangkhathat, Wararat Chiangjong, Kanitpong Phabphal

“…In this study, we performed proteomic analysis of astrocytoma tissue from patients with and without seizures using mass spectrometry-based techniques. We detected 131 differentially expressed proteins (42 upregulated and 89 downregulated). …”
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Incorporation of recombinant proteins into extracellular vesicles by Lactococcus cremoris by Tina Vida Plavec, Kristina Žagar Soderžnik, Giulia Della Pelle, Špela Zupančič, Robert Vidmar, Aleš Berlec

“…The presence of vesicular structures (50–200 nm) in the samples was confirmed by transmission electron microscopy and by flow cytometry using membrane-specific stain. Mass spectrometry analyses confirmed the presence of recombinant proteins in the EVs fraction, with amounts ranging from 13.17 to 100%, highlighting their significant incorporation into the vesicles, together with intrinsic L. cremoris NZ9000 proteins that were either more abundant in the cytoplasm (ribosomal proteins, metabolic enzymes) or present in the membrane. …”
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Systematic application of UPLC-Q-ToF-MS/MS coupled with chemometrics for the identification of natural food pigments from Davidson plum and native currant by Thomas Owen Hay, Melissa A. Fitzgerald, Joseph Robert Nastasi

“…The species were quantitatively assessed for colour properties using the CIELAB colour system in conjunction with Ultra Performance Liquid Chromatography-Quadrupole Time of Flight Tandem Mass Spectrometry (UPLC-Q-ToF-MS/MS). The results highlight diverse phenolic, flavonoid, and significant anthocyanin levels in Davidson plum and native currant, contributing to their robust red hues, comparable to commercial blueberry standards. …”
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Lipidomic Signature of Pregnant and Postpartum Females by Longitudinal and Transversal Evaluation: Putative Biomarkers Determined by UHPLC-QTOF-ESI⁺-MS by Alexandra Traila, Marius Craina, Carmen Socaciu, Andreea Iulia Socaciu, Diana Nitusca, Catalin Marian

“…While metabolomics has been utilized to study pregnancy-related metabolic changes, comprehensive comparisons between pregnant and non-pregnant states, particularly using ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS), remain limited. Methods: This study aimed to explore the dynamic, longitudinal metabolic shifts during pregnancy by profiling plasma samples from 65 pregnant women across three time points (6–14 weeks, 14–22 weeks, and >24 weeks) and 42 postpartum women. …”
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Fusarium oxysporum & Fusarium solani: Identification, Characterization, and Differentiation the Fungal Phenolic Profiles by HPLC and the Fungal Lipid Profiles by GC-MS by Nashwa M. Shalapy, Wenyi Kang

“…Moreover, lipid criteria of Fusarium oxysporum and Fusarium solani extracts had been displayed by gas chromatography-mass spectrometry (GC-MS) and their fatty acids had been identified to define the prolific species of the most biological and valuable fatty acids. …”
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Differentiation and characterization of volatile compounds in five common milk powders using HS-GC-IMS, HS-SPME-GC–MS, and multivariate statistical approaches by Yaxi Zhou, Diandian Wang, Jian Zhao, Yu Guo, Wenjie Yan

“…This study analysed and identified the flavor characteristics of five common types of milk powders in China, including yak milk powder, donkey milk powder, camel milk powder, goat milk powder, and cow milk powder, using Headspace-Gas Chromatography-Ion Mobility Spectrometry (HS-GC-IMS), Headspace Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry (HS-SPME-GC–MS), and multivariate statistical analysis. …”
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Detection of Bioactive Compounds Produced by in Vitro Culture of Jojoba Plants (Simmondsia chinesi (Link) Schn.) Using GCMS and FTIR by Aman H. A. Althuluth, Aqeel A. Suhaim, Murtadha S. Auda

“…The quality of the formed callus and the most important secondary compounds were analyzed using Gas chromatography–mass spectrometry (GC–MS)and Fourier-transform infrared spectroscopy (FTIR).The MS media(murashige and skoog) supplemented with 1 mg L-1 NAA + 2.5 mg L-1 TDZ and 2 mg L-1 NAA + 5 mg L-1 TDZ was most effective for callus formation, with the short period of time and the highest percentages of callus formation and fresh weight.GC-MS analysis identified various active compounds in the jojoba plant callus. …”
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Comparative Pharmacokinetic Study of Three Major Bioactive Components in the Normal and Different Courses of Liver-Stagnation and Spleen-Deficiency Syndrome Depressive Rats after I... by Kaiwen Luo, Mengdie Wang, Xiangxiang Pan, Yadong Xing

“…To reveal the pharmacokinetic process of narirutin, naringin, and honokiol in normal and different courses of liver-stagnation and spleen-deficiency syndrome depressive rats after intragastric administration of Zhi-Zi-Hou-Po decoction (ZZHPD), a rapid ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) method was proposed in this study. …”
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PFAS contamination in tap water: Target and suspect screening of zwitterionic, cationic, and anionic species across Canada and beyond by Termeh Teymoorian, Gabriel Munoz, Sébastien Sauvé

“…An additional ∼ 200 suspect-target PFAS were screened using high-resolution Orbitrap mass spectrometry. The results revealed widespread contamination of PFAS in tap water. …”
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