Isolation of PCSK9-specific nanobodies from synthetic libraries using a combined protein selection strategy by Apisitt Thaiprayoon, Yodpong Chantarasorn, Worrapoj Oonanant, Anongnard Kasorn, Phoomintara Longsompurana, Satita Tapaneeyakorn, Pinpunya Riangrungroj, Fabien Loison, Andrew C. Kruse, Matthew P. DeLisa, Dujduan Waraho-Zhmayev

“…Overall, our combined protein selection method enables rapid and straightforward identification of potent antigen-specific Nbs in a manner that can be executed in a basic laboratory setting without the need for specialized equipment. …”
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The in vitro anticancer effects of FS48 from salivary glands of Xenopsylla cheopis on NCI-H460 cells via its blockage of voltage-gated K+ channels by Xiong Weichen, Fan Huizhen, Zeng Qingye, Deng Zhenhui, Li Guanhui, Lu Wancheng, Zhang Bei, Lai Shian, Chen Xin, Xu Xueqing

“…The results demonstrated that FS48 can be effective in suppressing the Kv currents, migration, and invasion of NCI-H460 cells in a dose-dependent manner, despite the failure to inhibit the proliferation. …”
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Cannabidiol attenuates lipid metabolism and induces CB1 receptor-mediated ER stress associated apoptosis in ovarian cancer cells by Xuanhe Fu, Zhixiong Yu, Fang Fang, Weiping Zhou, Yuxin Bai, Zhongjia Jiang, Biao Yang, Ye Sun, Xing Tian, Guangyan Liu

“…In this study, we have demonstrated that CBD significantly inhibits the proliferation of OCs through a cannabinoid receptor type 1 (CB1R)-mediated manner. Fatty acid metabolic profiling and flow cytometry analysis revealed that CBD has the ability to decrease fatty acid levels and significantly suppress the transcription of genes involved in fatty acid uptake and synthesis in ES-2 cells. …”
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Follistatin-Like 1: A Potential Mediator of Inflammation in Obesity by Nengguang Fan, Haiyan Sun, Yufan Wang, Yifei Wang, Lijuan Zhang, Zhenhua Xia, Liang Peng, Yanqiang Hou, Weiqin Shen, Rui Liu, Jiajing Yin, Yongde Peng

“…The expression of proinflammatory mediators including IL-6, TNF-α, and MCP-1 was upregulated by recombinant FSTL1 in a dose-dependent manner, paralleled with activation of the IKKβ-NFκB and JNK signaling pathways in the two cell lines. …”
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Macrophages facilitate tumor cell PD‐L1 expression via an IL‐1β‐centered loop to attenuate immune checkpoint blockade by Cheng Xu, Yu Xia, Bai‐Wei Zhang, Emmanuel Kwateng Drokow, Hua‐Yi Li, Sen Xu, Zhen Wang, Si‐Yuan Wang, Ping Jin, Tian Fang, Xiao‐Ming Xiong, Pu Huang, Ning Jin, Jia‐Hong Tan, Qing Zhong, Yu‐Xin Chen, Qi Zhang, Yong Fang, Fei Ye, Qing‐Lei Gao

“…Specifically, IL‐1β released from TAMs was triggered by lactate, the anaerobic metabolite of tumor cells, in an inflammasome activation‐dependent manner. IL‐1β sustained and intensified immunosuppression by promoting C‐C motif chemokine ligand 2 secretion in tumor cells to fuel TAMs recruitment. …”
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Policy research on role of traditional medicine in emergency health system construction based on the PMC index model: evidence from China by Yujing Zhang, Xia Tian, Zhao Chen, Ziteng Hu, Huizhen Li, Xingyu Zong, An Li, Fuqiang Zhang, Yaxin Chen, Haili Zhang, Lijiao Yan, Ning Liang, Nannan Shi, Yanping Wang

“…This indicates that the policy quality related to TCM use in emergency response was normally distributed and generally qualified, although room for further improvement exists; policies should follow the principles of science, reasonableness, and operability, and should be updated in a timely manner with continuous development of the governance period while focusing on the policy content, safeguards, and role measures. …”
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Investigation study of face mask consumption in Malaysia and its impact on environment by Low Chung-Yu, Bee Soo-Tueen, Law Yung-Siang, Lee Tin-Sin, Bee Soo-Ling

“…Life Cycle Assessment (LCA) is carried out in this work to investigate the environmental impacts of both period of time by assessing the input and output of the face masks in a cradle-to-grave manner. OpenLCA software were used by adapting consequential system model based on ReCiPe 2016 (H) method as impact assessment method. …”
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What about the donor site morbidity – how invasive is the free latissimus dorsi flap? by Nikolaus Wachtel, Riccardo E. Giunta, Marc Hellweg, Maximilian Hirschmann, Constanze Kuhlmann, Nicholas Moellhoff, Denis Ehrl

“…Additionally, in 40 patients with free LDM flap surgery, these parameters were assessed in an identical manner. Results: We measured higher values for all parameters assessing force in the shoulder joint on the dominant side of patients in the control group. …”
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Trust Region Policy Learning for Adaptive Drug Infusion with Communication Networks in Hypertensive Patients by Mai The Vu, Seong Han Kim, Ha Le Nhu Ngoc Thanh, Majid Roohi, Tuan Hai Nguyen

“…Besides the various analyses such as normal condition (without CTD strategy), stability, and hybrid noise, a CTD analysis is implemented to illustrate the functionality of the system in a wireless manner and interruption in real-time feedback data.…”
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The histone demethylase KDM5C enhances the sensitivity of acute myeloid leukemia cells to lenalidomide by stabilizing cereblon by Lu Zou, Dan Cao, Qing Sun, Wenjun Yu, Bingzong Li, Guoqiang Xu, Liang Zhou

“…Biochemical experiments found that the CRBN-interacting protein KDM5C could stabilize CRBN and enhance the antileukemia effect of Len in an enzyme activity-independent manner. Furthermore, our studies revealed that the small-molecule compound MLN4924 could increase CRBN by elevating KDM5C.The combination of MLN4924 and Len can further increase the sensitivity of primary AML cells and AML cell lines to Len. …”
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Goat’s Milk Powder Enriched with Red (<i>Lycium barbarum</i> L.) and Black (<i>Lycium ruthenicum</i> Murray) Goji Berry Extracts: Chemical Characterization, Antioxidant Properties,... by Danijel D. Milinčić, Aleksandar Ž. Kostić, Steva Lević, Uroš M. Gašić, Dragana D. Božić, Relja Suručić, Tijana D. Ilić, Viktor A. Nedović, Bojana B. Vidović, Mirjana B. Pešić

“…GMLB and GMLR in a dose-dependent manner (0.3–5 mg/mL) showed a growth-promoting effect on probiotic strains. …”
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Niraparib restricts intraperitoneal metastases of ovarian cancer by eliciting CD36-dependent ferroptosis by Ning Jin, Yi-yu Qian, Xiao-fei Jiao, Zhen Wang, Xin Li, Wen Pan, Jin-kai Jiang, Pu Huang, Si-yuan Wang, Ping Jin, Qing-lei Gao, Dan Liu, Yu Xia

“…In the context of abundant fatty acids, niraparib elevated intracellular levels of fatty acids and lipid peroxidation, leading to subsequent tumor cell ferroptosis in a p53 and BRCA-independent manner. Notably, under niraparib exposure, a critical fatty acid transporter CD36 was dramatically upregulated in tumors, facilitating excessive uptake of fatty acids. …”
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Transverse Carpal Ligament and Forearm Fascia Release for the Treatment of Carpal Tunnel Syndrome Change the Entrance Angle of Flexor Tendons to the A1 Pulley: The Relationship bet... by Nazım Karalezli, Harun Kütahya, Ali Güleç, Serdar Toker, Hakan Karabörk, Tunc C. Ogun

“…We saw that the entrance angle increased in all of five fingers in an increasing manner from procedure 1 to 3, and it was seen that the maximal increase is detected in the middle finger from procedure 1 to procedure 2 and the minimal increase is detected in little finger. …”
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Developmental exposure to perfluorooctane sulfonate(PFOS) impairs the endometrial receptivity by Rui Ren, Xinyue Zhou, Tianyu Jia, Bin Wang, Ahui Liu, Min Gao, Ji Song, Liyan Wang, Yuanxue Jing, Liulin Yu, Haofei Shen, Xuehong Zhang

“…The activity against endometrial mesenchymal cells was significantly reduced by PFOS intervention, and the apoptosis flow assay results showed that PFOS significantly promoted cell death in a concentration-dependent manner. Transmission electron microscopy results revealed mitochondrial damage in the PFOS-intervened group, and WB results showed that the expression levels of endometrial tolerance-related proteins Homeobox A10 (HOXA10) and integrin beta3 (ITGB3) were decreased, and the expression level of Forkhead box O1 (FOXO1) protein was increased. …”
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Effect of Chromatin-Remodeling Agents in Hepatic Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells In Vitro and In Vivo by Danna Ye, Tong Li, Philip Heraud, Rangsun Parnpai

“…We conclude that HDACis enhance hepatic differentiation in a time-dependent manner, while DNMTis do not induce the hepatic differentiation of rBM-MSCs in vitro. …”
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Characterization of the procoagulant phenotype of amniotic fluid across gestation in rhesus macaques and humans by Chih Jen Yang, Lyndsey E. Shorey-Kendrick, Cristina Puy, Ashley E. Benson, Phillip A. Wilmarth, Ashok P. Reddy, Keith D. Zientek, Kilsun Kim, Adam Crosland, Chaevien S. Clendinen, Lisa M. Bramer, Olivia L. Hagen, Helen H. Vu, Joseph E. Aslan, Owen J.T. McCarty, Joseph J. Shatzel, Brian P. Scottoline, Jamie O. Lo

“…Conclusion: Overall, our findings reveal that the addition of AF to plasma enhances coagulation in a manner dependent on phospholipids as well as the presence of proteases and other proteins that directly regulate coagulation. …”
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PB: A Message Transmission Method Based on Area Layer Division in UAV Networks by Lisong Wang, Feifei Zhu, Qing Zhou, Kui Li, Liang Liu

“…Message transmission between area layers adopts a geographical routing manner, that is, messages are transmitted to the area layer closer to the ground station. …”
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Expression of a Splice Variant of CYP26B1 in Betel Quid-Related Oral Cancer by Ping-Ho Chen, Ka-Wo Lee, Cheng-Chieh Hsu, Jeff Yi-Fu Chen, Yan-Hsiung Wang, Ker-Kong Chen, Hui-Min David Wang, Hurng-Wern Huang, Bin Huang

“…Cytotoxicity assays were used to measure the effects of arecoline on cell viability in a dose-dependent manner. In vitro and in vivo studies were conducted to evaluate the expression of the CYP26B1 splice variant. …”
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Evaluating the antagonist effect of naltrexone implant via opioid challenge tests with escalating doses of hydromorphone injection in former heroin dependent patients by Wei Qu, Xuyi Wang, Chongyang Dong, Tao Zhang, Shugui Yin, Zhijun Sun, Shiqiang Wang, Anni Guo, Wei Hao

“…The findings indicate that the NXT-IMP effectively blocks the agonistic effects of hydromorphone in a dose-dependent manner.…”
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Synthesis and release profile of ibuprofen-loaded zein and gelatin nanofiber scaffolds for potential transdermal application in burn wound treatment by umaima saleem memon, Murk saleem memon, Abdul Wahab Memon, Mehwish Shahzad, Murk Rehman, Farooq Ahmed, Zeeshan Khatri

“…The results indicate that the Ibuprofen is efficiently loaded onto the nanofibers, with a uniform distribution of the drug throughout the fiber matrix and ensures that the drug is released in a controlled and sustained manner, promoting effective wound healing.…”
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