Ex vivo biotransformation of lady’s mantle extracts via the human gut microbiota: the formation of phenolic metabolites and their impact on human normal and colon cancer cell lines... by Katarzyna Jakimiuk, Aleksandra Kruk, Marta Kinga Lemieszek, Jakub W. Strawa, Sebastian Granica, Adrian Wiater, Michał Tomczyk

“…Samples from AVD1, AVD2, and AVD3 increase the lactate dehydrogenase (LDH) released from damaged colon cancer cells in a dose-dependent manner. At 250 μg/mL, AVD1, AVD2, and AVD3 elevated the LDH level by 12.6%, 25.3%, and 30.0%, respectively. …”
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Intercellular TIMP-1-CD63 signaling directs the evolution of immune escape and metastasis in KRAS-mutated pancreatic cancer cells by Chu-An Wang, Ya-Chin Hou, Yi-Kai Hong, Yu-Jing Tai, Chieh Shen, Pei-Chi Hou, Jhao-Lin Fu, Cheng-Lin Wu, Siao Muk Cheng, Daw-Yang Hwang, Yung-Yeh Su, Yan-Shen Shan, Shaw-Jenq Tsai

“…In vitro and in vivo studies reveal that early infiltrating macrophage-derived tissue inhibitor of metallopeptidase 1 (TIMP-1) is the key factor in maintaining the ERKactiveDUSP2low cell population in a CD63-dependent manner. The ERKactiveDUSP2low cancer cells further exacerbate macrophage-mediated cancer malignancy, including loss of epithelial trait, increased lymphangiogenesis, and immune escape. …”
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Normalization of tumor vasculature by imiquimod: proposal for a new anticancer therapeutic indication for a TLR7 agonist by Jarosz-Biej Magdalena, Czapla Justyna, Ciepła Joanna, Smolarczyk Ryszard, Drzyzga Alina, Sprus-Lipka Dorota, Pilny Ewelina, Matuszczak Sybilla, Cichoń Tomasz

“…Imiquimod normalized tumor vasculature both structurally (by reducing vessel tortuosity and increasing pericyte coverage) and functionally (by improving tumor perfusion) in a dose-dependent manner. Hypoxia regions in tumors of treated mice were significantly reduced after IMQ administration. …”
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Sandpiper Optimization Algorithm With Region Growing Based Robust Retinal Blood Vessel Segmentation Approach by Ibrahim AlMohimeed, Mohamed Yacin Sikkandar, A. Mohanarathinam, Velmurugan Subbiah Parvathy, Mohamad Khairi Ishak, Faten Khalid Karim, Samih M. Mostafa

“…This method has been specifically designed to improve the generalization capabilities and accuracy of retinal vessel segmentation manners, vital for the precise detection of retinal diseases like vein occlusion and DR. …”
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Effect of carbon quantum dots on the anticorrosive action of the tin coatings by Taiane Fíngolo Duarte, Victor Magno Paiva, Braulio Soares Archanjo, Clara Muniz da Silva de Almeida, Natasha Midori Suguihiro, Rodrigo José Corrêa, Emerson Schwingel Ribeiro, Eliane D'Elia

“…The SEM and XDR show that this concentration favored the growth of the film in a more uniform manner, providing a more homogeneous barrier when compared to the pure Sn film. …”
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MicroRNA-122 protects against interferon-α-induced hepatic inflammatory response via the Janus kinase–signal transducer and activator of transcription pathway by Fanwei Liu, Bowen Liu, Shanshan Xu, Yinhua Ni, Xiaoli Liu

“…Furthermore, silencing IFIT1 via an siRNA elicited an inflammatory response, whereas IFIT1 overexpression ameliorated hepatic inflammation and fibrosis in a manner comparable to that induced by IFN-α treatment. …”
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Rosiglitazone, a Ligand to PPARγ, Improves Blood Pressure and Vascular Function through Renin-Angiotensin System Regulation by María Sánchez-Aguilar, Luz Ibarra-Lara, Leonardo Del Valle-Mondragón, María Esther Rubio-Ruiz, Alicia G. Aguilar-Navarro, Absalom Zamorano-Carrillo, Margarita del Carmen Ramírez-Ortega, Gustavo Pastelín-Hernández, Alicia Sánchez-Mendoza

“…We conclude that RGZ lowers blood pressure values by increasing the expression of RAS axis proteins ACE2 and AT2R, decreasing the levels of AngII and increasing levels of Ang-(1-7) in a PPARγ-dependent manner. The in silico analysis is a valuable tool to predict the interaction between PPARγ and RAS.…”
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Comprehensive safety and toxicity analysis of 2,2’-Bipyridine derivatives in combating MRSA biofilm formation and persistence by Priyanka, Priyanka, Mohini Sharma, Bhavna Vaid, Bhavna Vaid, Ram Bharti, Ram Bharti, Sachin Raut, Sachin Raut, R. S. Jolly, Neeraj Khatri, Neeraj Khatri

“…They inhibited biofilm formation and eradicated biofilms in a dose-dependent manner against MTCC 737 and MRSA 831, and they effectively eliminated MRSA persister cells, outperforming the reference antibiotic vancomycin. …”
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Oncolytic peptide LTX-315 induces anti-pancreatic cancer immunity by targeting the ATP11B-PD-L1 axis by Meng Wang, Xing Huang, Tingbo Liang, Tianyu Tang, Gang Zhang, Minghao Lu, Zhengtao Hong, Junming Huang, Xiao Zhi

“…As regards the mechanism, ATP11B interacted with PD-L1 in a CKLF-like MARVEL transmembrane domain containing 6 (CMTM6)-dependent manner. The depletion of ATP11B promoted CMTM6-mediated lysosomal degradation of PD-L1, thus reactivating the immune microenvironment and inducing an antitumor immune response. …”
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A Phase I/II Study of Ultra-Hypofractionated Carbon-ion Radiation therapy for Low- and Intermediate-Risk Localized Prostate Cancer by Noriyuki Okonogi, MD, PhD, Hiroshi Tsuji, MD, PhD, Kana Kobayashi, MD, PhD, Mio Nakajima, MD, PhD, Shuri Aoki, MD, PhD, Takanobu Utsumi, MD, PhD, Hiroyoshi Suzuki, MD, PhD, Koichiro Akakura, MD, PhD, Tomohiko Ichikawa, MD, PhD, Hitoshi Ishikawa, MD, PhD

“…Ten patients were enrolled in each group, and the CIRT dose was increased in a stepwise manner if there were fewer than 4 cases of DLT. The initial CIRT dose was 36 Gy, followed by 40 Gy or 44 Gy. …”
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Quinazolinone Derivative MR2938 Protects DSS-Induced Barrier Dysfunction in Mice Through Regulating Gut Microbiota by Ling Lv, Mireguli Maimaitiming, Jichen Yang, Shuli Xia, Xin Li, Pingyuan Wang, Zhiqing Liu, Chang-Yun Wang

“…MR2938, a quinazolin-4(3H)-one derivative derived from the marine natural product penipanoid C, alleviated DSS-induced colitis in a dose-dependent manner. Herein, we aimed to investigate the impact of MR2938 on the gut microbiota in dextran sodium sulfate (DSS)-induced colitis in mice and to elucidate the role of the gut microbiota in the therapeutic mechanism of MR2938 for alleviating colitis. …”
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A clean energy Korea by 2035: Transitioning to 80% carbon-free electricity generation by Won Young Park, Yong Hyun Song, Nikit Abhyankar, Hee Seung Moon, Umed Paliwal, Eunsung Kim, Nina Khanna, Sanghyun Hong, James Hyungkwan Kim, Kenji Shiraishi, Jiang Lin, Seung Wan Kim, Amol Phadke

“…We find that accelerated renewable energy deployment by 2035 is achievable in a cost-effective and reliable manner, offering substantial economic, environmental, and energy security benefits. …”
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Quantitative proteomics and targeted metabolomics reveal the role of Cassia siamea L. extract in the response to high-fat diet-induced obesity in Drosophila by Ananya Dechakhamphu, Nattapong Wongchum, Theeraphan Chumroenphat, Alongklod Tanomtong, Sirithon Siriamornpun

“…In vitro, CSE significantly inhibited pancreatic lipase activity (IC50 = 67.73±3.24 µg/mL) in a noncompetitive manner. In a high-fat diet-fed Drosophila model, CSE supplementation (10 mg/mL) reduced lipase activity and triglyceride levels and improved body weight, suggesting antiobesity and potential antidiabetic effects. …”
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Enhancement of CD8+T cell cytotoxicity activity by IFN-α implies alternative pathologic role in systemic lupus erythematosus by Chen-xing Zhang, You-ying Mao, Yu-pin Tan, Mei-yu Zhang, Kang Shao, Shu-jun Wang, Ping Ji, Jia-yuan Wang, Lei Yin, Ying Wang

“…PI3K inhibitor reversed increased granzyme B synthesis upon IFN-α costimulation in a dose-dependent manner. Conclusion: In summary, elevated serum level of IFN-α was responsible for increased secretion of granzyme B and enhanced cytotoxicity of CD8+T cells in SLE and this process may be related to PI3K pathway. …”
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Ruxolitinib synergizes with regulatory T cells to improve inflammation but has no added benefits in decreasing albuminuria in SLE by Mi-Ae Lyu, Ximing Tang, Maria Gabriela Raso, Meixian Huang, Ke Zeng, Tara Sadeghi, Christopher R. Flowers, Simrit Parmar

“…A xenogeneic SLE model was utilized to study their in vivo combination.ResultsIn a dose-dependent manner, ruxolitinib addition synergizes with UCB-Tregs to suppress SLE-PBMC proliferation, inhibit CD8+ T cells, and reduce phosphorylation of STAT3/STAT5/AKT in CD8+ T cells. …”
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Platinum drugs upregulate CXCR4 and PD‐L1 expression via ROS‐dependent pathways, with implications for novel combined treatment in gastric cancer by Xiaoyu Kang, Lin Zhang, Shushang Liu, Fei Wang, Haiming Liu, Fenli Zhou, Fei Wu, Haohao Zhang, Daiming Fan, Yongzhan Nie, Zhangqian Chen

“…Increased levels of CXCR4 and PD‐L1 expression, in a dose‐ and time‐dependent manner upon cisplatin treatment, were observed in GC cells (p < 0.05). …”
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Hypermethylation in Calca Promoter Inhibited ASC Osteogenic Differentiation in Rats with Type 2 Diabetic Mellitus by Lei Wang, Feng Ding, Shaojie Shi, Xingxing Wang, Sijia Zhang, Yingliang Song

“…Meanwhile, it could significantly enhance the formation of ALP and calcium nodules in ASCs-T2DM, increase the expression of osteogenesis-related genes in vitro, and promote the healing of calvarial defects of T2DM rat in a concentration-dependent manner. 5-az intervention indicated that the reduction of the methylation level in Calca target fragment of ASCs-T2DM indeed escalated the gene expression, which may be related to DNMT1. …”
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PD-L1 positive platelets mediate resistance to immune checkpoint inhibitors in patients with colorectal cancer by Jiacheng Li, Jia Liu, Shifeng Yang, Yu Xia, Qingzhe Meng, Biying Sun, Yansong Liu, Bin Zhao, Jiaqi Jin, Hui Xu, Lihong Wang, Pengxia Zhang, Zhuoxin Cheng

“…Animal experiments revealed that antiplatelet pharmacological agents and genetic knockout of platelets potentiated the antitumour effect of the PD-L1 mAb treatment in a CD8 + T cell dependent manner. Conclusions Our data suggest that PD-L1 positive platelets suppress CD8 + T cell immunity. …”
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Combining In Vitro, In Vivo, and In Silico Approaches to Explore the Effect of <i>Ceratonia siliqua</i> and <i>Ocimum basilicum</i> Rich Phenolic Formula on Lipid Metabolism and Pl... by Mohammadine Moumou, Amani Tayebi, Abderrahmane Hadini, Omar M. Noman, Abdulsalam Alhalmi, Hamza Ahmoda, Souliman Amrani, Hicham Harnafi

“…Accordingly, the CBF prevented LRP oxidation in a concentration-dependent manner. This formula is not toxic in mice (LD₅₀ > 2000 mg/kg body weight). …”
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Interactive effect of dietary metabolizable energy levels with amino acid density in male broiler chickens: Carcass yield, nutrient intake, digestibility and excretion by Mehdi Toghyani, Shemil MacElline, Peter H. Selle, Sonia Y. Liu

“…Increasing AA density enhanced breast meat yield but increased white striping and woody breast scores in a linear manner (P < 0.01). Dietary treatments didn't affect ileal digestibility of crude protein and gross energy, except for improved starch digestibility with 6.0% AA density (P < 0.01). …”
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