Seq2scFv: a toolkit for the comprehensive analysis of display libraries from long-read sequencing platforms by Marianne Bachmann Salvy, Luca Santuari, Emanuel Schmid-Siegert, Nikolaos Lykoskoufis, Ioannis Xenarios, Bulak Arpat

“…Integrating high-throughput sequencing with computational approaches marks a pivotal advancement in antibody discovery, expanding the antibody space to explore. In this context, a major breakthrough has been the full-length sequencing of single-chain variable fragments (scFvs) used in in vitro display libraries. …”
Get full text

Clinical Characteristics and Management of the Hydatid Cyst of the Liver: A Study from a Tertiary Care Center in Nepal by Utsav Joshi, Roshan Subedi, Amar Jayswal, Vishakha Agrawal

“…Abdominal ultrasonography and computed tomography scan were the major imaging studies used to establish a diagnosis. …”
Get full text

Exploring influencing factors of healthy lifestyles in rural area among older adults with diabetes based on socioecological model by Yudong Miao, Xinran Li, Zhanlei Shen, Dongfang Zhu, Junwen Bai, Jingbao Zhang, Ruizhe Ren, Dan Guo, Clifford Silver Tarimo, Jinxin Cui, Mingyue Zhen, Jiajia Zhang, Yalin Song

“…In contrast, lower HLS were significantly associated with the presence of complications, depression, higher income, and residing farther from major roads. This study identified the key modifiable factors influencing HLS among older adults with diabetes. …”
Get full text

Targeting tRNA‐Derived Non‐Coding RNA Alleviates Diabetes‐Induced Visual Impairment through Protecting Retinal Neurovascular Unit by Jin Yao, Wen Yao, Jun‐Ya Zhu, Yan Liu, Jin‐Hong Liu, Yu‐Ke Ji, Xi‐Shen Ni, Wan Mu, Biao Yan

“…Abstract Diabetes is a major risk factor for compromised visual health, leading to retinal vasculopathy and neuropathy, both of which are hallmarks of neurovascular unit dysfunction. …”
Get full text

Multi-functional role of apolipoprotein E in neurodegenerative diseases by Sadequl Islam, Arshad Noorani, Yang Sun, Makoto Michikawa, Kun Zou

“…Genetic diversity in the apolipoprotein E (ApoE) gene has been identified as the major susceptibility genetic risk factor for sporadic Alzheimer’s disease (SAD). …”
Get full text

Falciparum Malaria in Febrile Patients at Sentinel Sites for Influenza Surveillance in the Central African Republic from 2015 to 2018 by Romaric Nzoumbou-Boko, Brice Martial Yambiyo, Carine Ngoagouni, Ulrich Vickos, Alexandre Manirakiza, Emmanuel Nakouné

“…Malaria is a major public health issue in the Central African Republic (CAR) despite massive scale-up of malaria interventions. …”
Get full text

Unraveling the degradation mechanism of multiple pyrethroid insecticides by Pseudomonas aeruginosa and its environmental bioremediation potential by Hui Liu, Wen-Juan Chen, Zeling Xu, Shao-Fang Chen, Haoran Song, Yaohua Huang, Kalpana Bhatt, Sandhya Mishra, Mohamed A. Ghorab, Lian-Hui Zhang, Shaohua Chen

“…UPLC-MS/MS analysis identified 2-(4-ethoxyphenyl) propan-2-ol and 3-phenoxybenzoic acid as the major metabolites of etofenprox biodegradation. A new esterase gene (estA) containing conserved motif (GDSL) and catalytic triad (Ser38, Asp310 and His313) was cloned from strain PAO1. …”
Get full text

A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells by Anna Babin-Ebell Gonçalves, Yifang Mao, Tinja Baljkas, Felix Wiedmann, Larissa Eis, Franziska Pilz, Manuel Winkler, Sina W. Kürschner-Zacharias, Marlene Hoffarth, Charlotta Funaya, Réza Shahidi, Cyrill Géraud, Chi-Chung Wu, Constanze Schmidt, Sergij Goerdt, Philipp-Sebastian Reiners-Koch, Mahak Singhal

“…However, the isolation of LSECs remains a major technological bottleneck in studying molecular mechanisms governing LSEC functions. …”
Get full text

Andrographolide Protects against HG-Induced Inflammation, Apoptosis, Migration, and Impairment of Angiogenesis via PI3K/AKT-eNOS Signalling in HUVECs by Ming-Xia Duan, Heng Zhou, Qing-Qing Wu, Chen Liu, Yang Xiao, Wei Deng, Qi-Zhu Tang

“…Andrographolide (Andr) is a major component isolated from the plant Andrographis paniculata. …”
Get full text

Hydroxide Sludge/Hydrochar-Fe Composite Catalysts for Photo-Fenton Degradation of Dyes by Zaina Izghri, Ghizlane Enaime, Mehdi Eouarrat, Lhoussaine Chahid, Layla El Gaini, Abdelaziz Baçaoui, Abdelrani Yaacoubi

“…The prepared composite was characterized by XRD, SEM, EDS, ICP, and FT-IR. The effect of major parameters, including pH, H2O2 concentration, and a dose of composite on the removal of MB has been studied. …”
Get full text

Pilot clinical trial of neoadjuvant toll-like receptor 7 agonist (Imiquimod) immunotherapy in early-stage oral squamous cell carcinoma by Angela J. Yoon, Richard D. Carvajal, Evan M. Graboyes, John M. Kaczmar, William G. Albergotti, Alexandra E. Kejner, Scott H. Troob, Elizabeth Philipone, Jean-Sebastien Anoma, Kent E. Armeson, Elizabeth G. Hill, Mary S. Richardson, Tina R. Woods, Bhishamjit S. Chera, Farzad Nourollah-Zadeh, Byung J. Lee, Subramanya Pandruvada, Antonis Kourtidis, Christina Kingsley, Elizabeth C. O’Quinn, Stephanie Mills, Victoria C. Jordan, Mike Spencer, Danielle Fails, Trevor D. McKee, Mark Zaidi, Alan Brisendine, Shane Horn, Shikhar Mehrotra, Besim Ogretmen, Jason G. Newman

“…Similarly, 60% of patients had immune-related major pathologic response (irMPR) with two complete pathologic responses, and 40% had partial response (PR) with the percent residual viable tumor ranging from 25% to 65%. …”
Get full text

Tenascin C-Guided Nanosystem for Precision Delivery of Obeticholic Acid in Liver Fibrosis Therapy by Yawen Wang, Lei Yang, Qing Xu, Taiyu Liu, Hongliang He, Lisha Liu, Lifang Yin

“…The dense fibrotic ECM acts as a major physiological barrier, necessitating the development of a targeted delivery strategy to achieve effective therapeutic outcomes. …”
Get full text

Novel Excavation and Construction Method for a Deep Shaft Excavation in Ultrathick Aquifers by Chengyong Cao, Chenghua Shi, Linghui Liu, Jianwen Liu, Mingfeng Lei, Yuexiang Lin, Yichao Ye

“…The shaft excavation with the depth of 41.6 m below the ground surface (BGS) is divided into two major phases, that is, (1) the first part of the excavation (the depth of 23.6 m BGS) is conducted by the way of conventional dewatering and braced excavation (Phase I) and (2) the second excavation with the depth of 23.6 m to 41.6 m BGS is carried out by the novel underwater excavation without drainage technique (Phase II). …”
Get full text

A systematic review of Aspilia africana (Pers.) C.D. adams traditional medicinal uses, phytoconstituents, bioactivities, and toxicities by Gang, Roggers, Okello, Denis, Yeongjun, Ban, Kang, Youngmin

“…The extracts and bioactive phytochemicals of A. africana have revealed several pharmacological prop-erties, including antimalarial, anticancer, wound healing, anti-inflammatory, antidiabetic, and antimicrobial activities. However, the major components responsible for these bioactivities and their mechanisms of action in some diseases have not yet been clearly identified. …”
Get full text

Exogenous Interleukin-37 Alleviates Hepatitis with Reduced Dendritic Cells and Induced Regulatory T Cells in Acute Murine Cytomegalovirus Infection by Yufei Ruan, Zhengwang Wen, Ke Chen, Jianan Xi, Bo Wu, Zhiyong Xu, Minzhi Jiang, Junling Zhang, Yiping Chen, Qi Liu

“…Human cytomegalovirus (HCMV) infection is globally distributed, and the liver is one of the major targeting organs. So far, the mechanisms for cell and organ damage have not fully been elucidated and the treatments for the infection are mainly at symptoms. …”
Get full text

Expression of EZH2 and H3K27me3 predicts tumor biology of urothelial carcinoma by Rasheeda Mohamedali, Suvradeep Mitra, Swarnendu Mandal, Prasant Nayak, Amit K. Adhya, Suvendu Purkait

“…Background: Enhancer of zeste homolog 2 (EZH2) is one of the major epigenetic modifiers involved in the transcriptional repression of target genes through trimethylation of H3K27 (lysine 27 residue of histone H3). …”
Get full text

Research on Seepage Characteristics of Y-Shaped Fractures under Different Fracture Roughness by Ruifeng Tang, Jing Xie, Shouning Xue, Zhilong Zhang, Junjun Liu, Bengao Yang

“…And there is a threshold DM between 1.1 and 1.2 so that the major influencing factor on hydraulic conductivity changes. …”
Get full text

Cyanobacteria cultivation using olive milling wastewater for bio-fertilization of celery plant by S. Rashad, A.S. El-Hassanin, S.S.M. Mostafa, G.A. El-Chaghaby

“…Olive milling wastewater is a major problem facing the Mediterranean countries producing olive oil like Egypt. …”
Get full text

Turkish suicide patterns: Longitudinal analysis of suicide trends in Turkiye (2000 -2022) by Ayhan Tabur, Feruza Turan Sonmez

“…Suicide is a major global public health issue and a growing concern in Turkiye, driven by demographic and societal changes. …”
Get full text

SEVERE CONSTRICTED HEAD – AN EXTREME FORM OF CROUZON SYNDROME POSING CHALLENGING FRONTO-ORBITAL ADVANCEMENT: A CASE SERIES REPORTS by Ciptomurti Lupitasari, Lobredia Zarasade, Magda Rosalina Hutagalung

“…Crouzon syndrome with acanthosis nigricans is distinct from the classic Crouzon syndrome, characterized by thick and dark skin in body folds. The major problems resulting from constricted head are related to craniostenosis, orbitostenosis developing from abnormalities of the skull base associated with progressive rise in intracranial and intraorbital pressures which could progress to hydrocephalus and cerebellar herniation. …”
Get full text