Loss of MEF2C function by enhancer mutation leads to neuronal mitochondria dysfunction and motor deficits in mice by Ali Yousefian-Jazi, Suhyun Kim, Jiyeon Chu, Seung-Hye Choi, Phuong Thi Thanh Nguyen, Uiyeol Park, Min-gyeong Kim, Hongik Hwang, Kyungeun Lee, Yeyun Kim, Seung Jae Hyeon, Hyewhon Rhim, Hannah L. Ryu, Grewo Lim, Thor D. Stein, Kayeong Lim, Hoon Ryu, Junghee Lee

“…To examine the alteration of MEF2C transcription by the SNP, we generated HEK293T cells carrying the major or minor allele by CRISPR-Cas9. To verify the role of MEF2C-knockdown (MEF2C-KD) in mice, we developed AAV expressing shRNA for MEF2C based on AAV-U6 promoter vector. …”
Get full text

Association between cadherin-related family member 3 rs6967330-A and human rhinovirus-C induced wheezing in children by Hanhaoyu Fu, Ri De, Yu Sun, Yao Yao, Runan Zhu, Dongmei Chen, Yutong Zhou, Qi Guo, Linqing Zhao

“…Human rhinoviruses (HRV) are the major pathogens in severe wheezing in young children. …”
Get full text

Multi-omics dissection of metabolic dysregulation associated with immune recovery in people living with HIV-1 by Lin-Yu Wan, Sin Man Lam, Hui-Huang Huang, Wen-Jing Cao, Xiang-Yi Cao, Xue-Meng Li, Li-Ping Zhang, Jia-Min Gao, Chao Zhang, Xing Fan, Yan-Mei Jiao, Guanghou Shui, Fu-Sheng Wang, Jin-Wen Song

“…Pathway analysis uncovered the biosynthesis of unsaturated fatty acids as the major dysregulated pathway in TNs, IRs, and INRs, while primary bile acid biosynthesis was the dysregulated pathway specifically in INRs. …”
Get full text

Orbital cycle records in shallow unconsolidated sediments: implications for global carbon cycle and hydrate system evolution in deep-sea area sediments of the Qiongdongnan Basin by Yang Dong, Yang Dong, Ren Wang, Ren Wang, Jinqiang Liang, Yulin He, Jinfeng Ren, Wanzhong Shi, Wanzhong Shi, Xiaosong Wei, Xiaosong Wei, Hao Du, Hao Du, Xiangyang Xie, Arthur B. Busbey

“…Simultaneously, through the analyses of major trace elements and total organic carbon content (TOC) in sediment samples, how astronomical orbital cycles influenced past environmental conditions. …”
Get full text

Long-Term Clinical Outcomes for Non-ST Elevation Acute Coronary Syndrome Patients with High-Risk Angiographic Findings Undergoing Percutaneous Coronary Intervention by Sida Jia, Ce Zhang, Yue Liu, Deshan Yuan, Xueyan Zhao, Runlin Gao, Yuejin Yang, Bo Xu, Zhan Gao, Jinqing Yuan

“…Prognosis impact on 2-year and 5-year major adverse cardiovascular and cerebrovascular events (MACCE) is analyzed. …”
Get full text

The use of antimicrobials in Italian buffalo farms by Gabriele Di Vuolo, Federico Scali, Claudia Romeo, Valentina Lorenzi, Chiara D. Ambra, Maria Serrapica, Giovanna Cappelli, Francesca Fusi, Esterina De Carlo, Giovanni Loris Alborali, Luigi Bertocchi, Domenico Vecchio

“… Antimicrobial resistance (AMR) is a major global health emergency; 25,000 people die each year in Europe from infections sustained by resistant bacteria. …”
Get full text

Etoposide targets 2A protease to inhibit enterovirus 71 replication by Qinqin Liang, Sai Shi, Qingjie Zhang, Yaxin Wang, Sheng Ye, Binghong Xu

“…ABSTRACT Enterovirus 71 (EV71) is a major pathogen that causes hand, foot, and mouth disease (HFMD) in infants and children. …”
Get full text

Comprehensive analysis of antimicrobial resistance dynamics among broiler and duck intensive production systems by Zsombor Szoke, Peter Fauszt, Maja Mikolas, Peter David, Emese Szilagyi-Tolnai, Georgina Pesti-Asboth, Judit Rita Homoki, Ildiko Kovacs-Forgacs, Ferenc Gal, Laszlo Stundl, Levente Czegledi, Aniko Stagel, Sandor Biro, Judit Remenyik, Melinda Paholcsek

“…Despite its limitations, this research provides key insights into AMR trends in two major poultry types, guiding targeted interventions and sustainable management strategies.…”
Get full text

Social determinants of mental health in asthma: an exploratory study by Sarah A. Hiles, Sarah A. Hiles, Hayley Lewthwaite, Hayley Lewthwaite, Vanessa L. Clark, Vanessa L. Clark, Anne E. Vertigan, Anne E. Vertigan, Anne E. Vertigan, Amber Smith, Amber Smith, Vanessa M. McDonald, Vanessa M. McDonald, Vanessa M. McDonald

“…Participants were on average 60 years old (SD = 14), 72% were female, 94% were Caucasian, 94% were Australian-born, 26% lived alone, 42% were working full- or part-time, and 83% lived in a major city of Australia. Anxiety and depression were relatively common (35% anxiety; 16% depression using HADS threshold of scores ≥8). …”
Get full text

Genomic epidemiology of third-generation cephalosporin-resistant Escherichia coli from companion animals and human infections in Europe by Adrien Biguenet, Benoit Valot, Farid El Garch, Xavier Bertrand, Didier Hocquet

“…Although it cannot rule out occasional transmission of bacteria between pets and humans within a household, it suggests that dogs and cats are not a major source of human infection with this antibiotic-resistant pathogen.…”
Get full text

Longitudinal exploration of cancer-related cognitive impairment in patients with newly diagnosed aggressive lymphoma: protocol for a feasibility study by Vincent Dore, Christopher C Rowe, Meinir Krishnasamy, Haryana Dhillon, Adam K Walker, Karla Gough, Priscilla Gates, Carlene Wilson, Eliza Hawkes, Yuliya Perchyonok, Janette L Vardy, Michiel de Ruiter

“…For some patients, their cognitive impairment may be transient, but for a subgroup, these symptoms can be long-standing and have a major impact on the quality of life. This paper describes the protocol for a study: (1) to assess the feasibility of collecting longitudinal data on cognition via self-report, neuropsychological testing, peripheral markers of inflammation and neuroimaging and (2) to explore and describe patterns of cancer-related cognitive impairment over the course of treatment and recovery in patients with newly diagnosed, aggressive lymphoma undergoing standard therapy with curative intent.Methods and analysis This is a prospective, longitudinal, feasibility study in which 30 newly diagnosed, treatment-naive patients with aggressive lymphoma will be recruited over a 12-month period. …”
Get full text

Probing the Dual Role of Ca²⁺ in the <i>Allochromatium tepidum</i> LH1–RC Complex by Constructing and Analyzing Ca²⁺-Bound and Ca²⁺-Free LH1 Comple... by Mei-Juan Zou, Shuai Sun, Guang-Lei Wang, Yi-Hao Yan, Wei Ji, Zheng-Yu Wang-Otomo, Michael T. Madigan, Long-Jiang Yu

“…Crucially for the ecological success of <i>Alc. tepidum</i>, Ca²⁺-bound LH1 complexes were more thermostable than Ca²⁺-free complexes, indicating that calcium plays at least two major roles in photosynthesis by <i>Alc. tepidum</i>—improving photocomplex stability and modifying its spectrum.…”
Get full text

Impacts of Recent Climate Trends and Human Activity on the Land Cover Change of the Abbay River Basin in Ethiopia by Asaminew Abiyu Cherinet, Denghua Yan, Hao Wang, Xinshan Song, Tianlin Qin, Mulualem T. Kassa, Abel Girma, Batsuren Dorjsuren, Mohammed Gedefaw, Hejia Wang, Otgonbayar Yadamjav

“…The Abbay River Basin, which originates in Ethiopia, is a major tributary and main source of the Nile River Basin. …”
Get full text

Knowledge, attitude and practices regarding vitamin D among adults in Ghana: a cross-sectional study by Abraham Ameyaw Kwabena, Benedicta Appiah, Samuel Ankomah Danso, Samuel Kwame Sopuruchi Agomuo, Samuel Kwarteng, Ebenezer Senu, Alfred Effah, Samuel Asamoah Sakyi, Linda Ahenkorah Fondjo

“…Abstract Background Vitamin D deficiency is a major public health concern, affecting approximately half of the world's population, partly due to limited public knowledge about vitamin D sources. …”
Get full text

Mechanical Ventilator-Associated Pneumonia in the COVID-19 Pandemic Era: A Critical Challenge in the Intensive Care Units by Mircea Stoian, Adina Andone, Sergiu Rareș Bândilă, Danusia Onișor, Sergiu Ștefan Laszlo, Gabriela Lupu, Alina Danielescu, Dragoș-Florin Baba, Anca Meda Văsieșiu, Andrei Manea, Adina Stoian

“…We evaluated the causative pathogens involved and their resistance to the major classes of antibiotics in patients with VAP and assessed the differences between patients with and without coronavirus disease 2019 (COVID-19). …”
Get full text

TCR-engaging scaffolds selectively expand antigen-specific T-cells with a favorable phenotype for adoptive cell therapy by Marco Donia, Özcan Met, Julie Westerlin Kjeldsen, Sine Reker Hadrup, Inge Marie Svane, Arianna Draghi, Anders Handrup Kverneland, Christina Heeke, Siri Amanda Tvingsholm, Marcus Svensson Frej, Vibeke Mindahl Rafa, Ulla Kring Hansen, Maria Ormhøj, Alexander Tyron, Agnete W P Jensen, Mohammad Kadivar, Amalie Kai Bentzen, Kamilla K Munk, Gitte N Aasbjerg, Jeppe S H Ternander, Tripti Tamhane, Christian Schmess, Samuel A. Funt, Søren Nyboe Jakobsen

“…Here, in this study, we present a novel technology for ex vivo expansion of antigen-specific T-cells; artificial antigen-presenting scaffolds (Ag-scaffolds) consisting of a dextran-polysaccharide backbone, decorated with combinations of peptide-Major Histocompatibility Complex (pMHC), cytokines and co-stimulatory molecules, enabling coordinated stimulation of antigen-specific T-cells.Methods The capacity of Ag-scaffolds to expand antigen-specific T-cells was explored in ex vivo cultures with peripheral blood mononuclear cells from healthy donors and patients with metastatic melanoma. …”
Get full text

Investigation of the Effects and Degradation Mechanism of Low-oxygen on the Stability of Blueberry Anthocyanins in Juice Simulation system by Zhouyang SHENG, Gengsheng XIAO, Lijuan ZHANG, Yujuan XU, Jijun WU, Yuanshan YU, Tenggen HU, Bo ZOU

“…Comparative analysis revealed that the degradation products of paeoniflorin under conventional conditions were mainly quercetin and vanillic acid, whereas the major degradation products of paeoniflorin in low-oxygen environment were vanillic acid and coumarin derivatives, and the degradation products of cornflowerin were protocatechuic acid regardless of oxygen conditions.…”
Get full text

Acceptability and feasibility of two interventions in the MooDFOOD Trial: a food-related depression prevention randomised controlled trial in overweight adults with subsyndromal sy... by Ulrich Hegerl, Edward Watkins, Miquel Roca, Matthew Owens, Mariska Bot, Ingeborg Annemarie Brouwer, Elisabeth Kohls, Brenda Penninx, Gerard van Grootheest, Mieke Cabout, Margalida Gili, Marjolein Visser, Bep Verkerk, Nadine Paans, Carisha Thesing, Deborah Gibson-Smith, Melany Horsfall, Lena Weiss, Amy Romijn, Hannah Bunce, Owain Winfield, Harriet Bunker-Smith, Fern Durbridge, Caterina Versari Molinares, Atikah Sapar, Miquel Tortella, Clara Homar Covas, M Angeles Pérez-Ara, Adoración Castro Gracia, José Luis Reig, Jana Hoesel, Ezgi Dogan, Sabrina Baldofski, Nicole Mauche

“…We also assessed baseline determinants of adherence and assessed whether better adherence resulted in lower depressive symptoms.Design Randomised controlled trial with a 2×2 factorial design conducted between 2015 and 2017.Setting Germany, the Netherlands, UK and Spain.Participants Community sample of 1025 overweight adults with elevated depressive symptoms without a current episode of major depressive disorder. Main eligibility criteria included age (18–75 years), being overweight or obese, and having at least mild depressive symptoms, shown by a Patient Health Questionnaire Score of ≥5. …”
Get full text

Micronutrient levels of global tropical reef fish communities differ from fisheries capture by Conor Waldock, Eva Maire, Camille Albouy, Vania Andreoli, Maria Beger, Thomas Claverie, Katie L. Cramer, David A. Feary, Sebastian C. A. Ferse, Andrew Hoey, Nicolas Loiseau, M. Aaron MacNeil, Matthew McLean, Camille Mellin, Simon Ahouansou Montcho, Maria Lourdes Palomares, Santiago de laPuente, Mark Tupper, Shaun Wilson, Laure Velez, Jessica Zamborain‐Mason, Dirk Zeller, David Mouillot, Loïc Pellissier

“…Our results remained unchanged after accounting for the under‐sampling of fish communities and under‐reporting of small‐scale fisheries catches—two major sources of uncertainty. This reported mismatch indicates that current estimates of fished micronutrients are not adequate to fully assess micronutrient inventories. …”
Get full text

Risk factors for the delayed discharge from anesthesia intensive care unit: a single-center retrospective study by Yue Qian, Jing Hao, Wei Zhu, Yan Yang, Zhuan-yun Zhang, Lu-yang Zhou, Xiao-ping Gu, Zheng-liang Ma, Yu-e Sun

“…Postoperative hemorrhage was the major cause of delayed discharge from an AICU, followed by septic shock, hypoperfusion and pulmonary insufficiency. …”
Get full text