Standardization and validation of a novel reverse transcriptase polymerase chain reaction method for detecting virulent strains of the infectious bursal disease virus by Vladimir Longa-Bobadilla, Phillip Ormeño-Vásquez, Manuel Criollo-Orozco, Luis Tataje-Lavanda, Katherine Huamán-Gutierrez, Ángela Montalván, Mirko Zimic, Manolo Fernández-Sanchez, Manolo Fernández-Díaz

“…For many years, the development of molecular techniques for either diagnostic purposes or surveillance of the appearance of new pathogenic strains has mainly focused on targeting the VP2 genomic region. …”
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Los olvidados: Non-BRCA variants associated with Hereditary breast cancer in Mexican population by Dione Aguilar, María Lourdes Garza-Rodríguez, Carolina Elizabeth Muñiz-Garza, Fernando Alcorta Nuñez, Cynthia Mayte Villarreal-Garza, Oscar Vidal-Gutiérrez, Diana Cristina Pérez-Ibave, Carlos Horacio Burciaga-Flores

“…HBOC pathogenesis is caused mainly by germline pathogenic variants (GPV) in BRCA1 and BRCA2 genes. …”
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Society for Immunotherapy of Cancer: updates and best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) image analysis and data sharing by Ignacio I Wistuba, Michael T Tetzlaff, Carlo B Bifulco, Benjamin Green, Sacha Gnjatic, Scott J Rodig, Joel C Sunshine, Janis M Taube, Keith E Steele, Marlon C Rebelatto, David L Rimm, Ana Lako, Edwin R Parra, Guray Akturk, Michael Angelo, Shirley Greenbaum, Noah F Greenwald, Cyrus V Hedvat, Travis J Hollmann, Kurt A Schalper, Cláudia S Ferreira, Konstanty Korski, Emanuel Schenck, Michael J Surace, Jennifer H Yearley, Jeffrey S Roskes, Margaret Eminizer, Logan L Engle, Daniel Jiménez-Sánchez, Jamie Rodriguez-Canales, Alexander S Szalay

“…While the current effort is mainly focused on best practices for characterizing the tumor microenvironment, these principles are broadly applicable to any mIHC/IF assay and associated image analysis.…”
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Progress in voluntary medical male circumcision for HIV prevention supported by the US President’s Emergency Plan for AIDS Relief through 2017: longitudinal and recent cross-sectio... by Carlos Toledo, Melissa Habel, Stephanie M Davis, Jonas Z Hines, Jonathan M Grund, Renee Ridzon, Brittney Baack, Jonathan Davitte, Anne Thomas, Valerian Kiggundu, Naomi Bock, Paran Pordell, Caroline Cooney, Irum Zaidi

“…Objective This article provides an overview and interpretation of the performance of the US President’s Emergency Plan for AIDS Relief’s (PEPFAR’s) male circumcision programme which has supported the majority of voluntary medical male circumcisions (VMMCs) performed for HIV prevention, from its 2007 inception to 2017, and client characteristics in 2017.Design Longitudinal collection of routine programme data and disaggregations.Setting 14 countries in sub-Saharan Africa with low baseline male circumcision coverage, high HIV prevalence and PEPFAR-supported VMMC programmes.Participants Clients of PEPFAR-supported VMMC programmes directed at males aged 10 years and above.Main outcome measures Numbers of circumcisions performed and disaggregations by age band, result of HIV test offer, procedure technique and follow-up visit attendance.Results PEPFAR supported a total of 15 269 720 circumcisions in 14 countries in Southern and Eastern Africa. …”
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Application of artificial intelligence brain structure-based paradigm to predict the slip condition impact on magnetized thermal Casson viscoplastic fluid model under combined temp... by Umar Farooq, Shan Ali Khan, Haihu Liu, Muhammad Imran, Lotfi Ben Said, Aleena Ramzan, Taseer Muhammad

“…One of the main reasons for the popularity of ANNs is their wonderful capability to handle very complex and nonlinear mathematical problems. …”
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The microbiota-derived bile acid taurodeoxycholic acid improves hepatic cholesterol levels in mice with cancer cachexia by Morgane M. Thibaut, Martin Roumain, Edwige Piron, Justine Gillard, Axelle Loriot, Audrey M. Neyrinck, Julie Rodriguez, Isabelle Massart, Jean-Paul Thissen, Joshua R. Huot, Fabrizio Pin, Andrea Bonetto, Nathalie M. Delzenne, Giulio G. Muccioli, Laure B. Bindels

“…Using three mouse models of cancer cachexia (the C26, MC38 and HCT116 models), we evidenced a reduction in the hepatic levels of several secondary bile acids, mainly taurodeoxycholic (TDCA). This reduction in hepatic TDCA occurred before the appearance of cachexia. …”
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Trends in the Use of Sphingosine 1 Phosphate in Age-Related Diseases: A Scientometric Research Study (1992-2020) by Qiong He, Gaofeng Ding, Mengyuan Zhang, Peng Nie, Jing Yang, Dong Liang, Jiaqi Bo, Yi Zhang, Yunfeng Liu

“…This study includes a discussion of the (1) historical overview of the topic; (2) main contributors: journals, countries, institutes, funding agencies, and authors; (3) collaboration between institutes and authors; (4) research hot spots and zones; and 5) research trends and frontiers. …”
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The transcriptional regulator Lrp activates the expression of genes involved in the biosynthesis of tilimycin and tilivalline enterotoxins in Klebsiella oxytoca by Miguel A. De la Cruz, Hilda A. Valdez-Salazar, Diana Rodríguez-Valverde, Santa Mejia-Ventura, Nayely Robles-Leyva, Tania Siqueiros-Cendón, Quintín Rascón-Cruz, Nancy León-Montes, Jorge Soria-Bustos, Fernando Chimal-Cázares, Roberto Rosales-Reyes, María L. Cedillo, Jorge A. Yañez-Santos, J. Antonio Ibarra, Javier Torres, Jorge A. Girón, James G. Fox, Miguel A. Ares

“…Altogether, our data present a new perspective on the role of Lrp as a regulator in cytotoxin-producing K. oxytoca strains and how it controls the expression of genes involved in the biosynthesis of their main virulence factors.IMPORTANCETilimycin (TM) and tilivalline (TV) are enterotoxins that are a hallmark for the cytotoxin-producing Klebsiella oxytoca strains, which cause antibiotic-associated hemorrhagic colitis. …”
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Composite Flours Based on Black Lentil Seeds and Sprouts with Nutritional, Phytochemical and Rheological Impact on Bakery/Pastry Products by Christine (Neagu) Dragomir, Sylvestre Dossa, Călin Jianu, Ileana Cocan, Isidora Radulov, Adina Berbecea, Florina Radu, Ersilia Alexa

“…The increase in micronutrient intake by fortifying wheat flour with black lentil flour in both germinated and ungerminated form is more significant compared to the increases recorded in the case of the main macronutrients (Ca, Na, Mg, and K). The micronutrients increased in the composite flours in the order: Cu < Zn < Fe < Mn. …”
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Comparing International Guidelines for the Remission of Hypertension After Bariatric Surgery by Carina Vieira Dias, Ana Lúcia Silva, Joana Dias, Paulo Cardoso, Rute Castanheira, Andreia Fernandes, Filipa Nunes, Tina Sanai, Mercedes Sanchez, João Maia-Teixeira, Ana Luísa De Sousa-Coelho

“…Dissimilar hypertension guidelines are available for clinicians, namely those prepared by the American Heart Association (AHA) and the European Society of Cardiology (ESC), which may lead to distinctive appreciation of health outcomes of patients with obesity after bariatric and metabolic surgery, such as hypertension remission. The main goal of this study was to compare the effects of applying stricter (AHA) versus looser (ESC) blood pressure criteria on hypertension diagnosis pre-bariatric surgery and remission assessment one year post-op. …”
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Exploring the relationship between proactive inhibition and restrictive eating behaviours in severe and enduring anorexia nervosa (SE-AN) by Savani Bartholdy, Bethan Dalton, Samantha J. Rennalls, Maria Kekic, Jessica McClelland, Iain C. Campbell, Owen G. O’Daly, Ulrike Schmidt

“…Results Both SE-AN and HC participants showed slower reaction times under conditions of uncertainty, indicating proactive inhibition in both groups. There was a main effect of group, with SE-AN participants showing significantly slower reaction times compared to HC. …”
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Chemoimmunotherapy as induction treatment in concurrent chemoradiotherapy for patients with nasopharyngeal carcinoma stage IVa by Kun-Peng Wu, Qing-Qing Li, Xu-Qiang Luo, Xiao-Xi Wang, Yan-Zhen Lai, Dan Tian, Hong-cheng Yang, Xue-Ling Wei, Lan-Ying Wang, Qiu-Ming Li, Dao Zhu, Si-Jie Chen, Yang-Si Li

“…Background Chemoimmunotherapy is the first-line therapy for patients with recurrent or metastatic nasopharyngeal carcinoma (NPC) and is currently the main induction treatment option for patients with locoregionally advanced NPC. …”
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Clinical outcome of non-alcoholic fatty liver disease: an 11-year follow-up study by Steven Dooley, Juan Du, Xiaoping Tang, Lan Chen, Jinzhu Jia, Fujun Li, Yanyan Shi, Christoph Meyer, Zhongwei Zhu, Yanming Zhang, Roman Liebe, Keming Hu, Tingting Zhou, Huier Zhang, Hong-Lei Weng, Tong Huang

“…Except one male patient who developed cirrhosis, no patients with NAFLD progressed into severe liver disease.Conclusion Diabetes, hypertension and hyperuricaemia are the main clinical outcomes of NAFLD. Eleven years of NAFLD are not sufficient to cause severe liver disease. …”
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Analysis of the dynamics of occupational morbidity and its consequences in Ukraine in comparison with other countries of the world by M.H. Prodanchuk, A.V. Basanets, O.P. Kravchuk, K.Y. Hashynova, V.A. Hvozdetskyy

“…According to the State Statistics Committee of Ukraine, the number of people working in the main sectors of the economy in conditions that do not meet sanitary and hygienic standards and requirements is 836,600 people, or 28.4% of the registered number of full-time employees. …”
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Evaluation of Wisconsin and CaPTHUS Indices Usefulness for Predicting Monoglandular and Multiglandular Disease in Patients with Primary Hyperparathyroidism through the Analysis of... by Loredana De Pasquale, Eleonora Lori, Antonio Mario Bulfamante, Giovanni Felisati, Luca Castellani, Alberto Maria Saibene

“…Background. The main challenge for treating primary hyperparathyroidism (PHPT) is to understand if it is caused by a single adenoma (80–85% of the cases) or by a multiglandular disease (15–20%), both preoperatively and intraoperatively. …”
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Dynamic rationing using agent-based modeling of the assembly process of equipment for nuclear power plants by I. A. Loskutov, D. A. Skvortsova, V. G. Iskandarova

“…There is a study of the use of models of a typical assembly process — the assembly of a cabinet frame, consisting of seven main operations. The paper presents structured, empirically analyzed values of fatigue categories and process time for 85 employees in graphical form by operations. …”
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Emergence of Carbapenem-Resistant <i>bla</i>_POM-1 Harboring <i>Pseudomonas otitidis</i> Isolated from River Water in Ghana by Frederick Ofosu Appiah, Samiratu Mahazu, Isaac Prah, Taira Kawamura, Yusuke Ota, Yohei Nishikawa, Mitsunori Yoshida, Masato Suzuki, Yoshihiko Hoshino, Toshihiko Suzuki, Tomoko Ishino, Anthony Ablordey, Ryoichi Saito

“…However, its prevalence in Ghana is unknown, mainly due to misidentification or inadequate research. …”
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Prevalence of Diabetes and Associated Risk Factors among a Group of Prisoners in the Yaoundé Central Prison by Sylvain Raoul Simeni Njonnou, Jérôme Boombhi, Martine Claude Etoa Etoga, Aimée Tiodoung Timnou, Ahmadou Musa Jingi, Kevin Nkem Efon, Esther Astrid Mbono Samba Eloumba, Marie-Josiane Ntsama Essomba, Odette Kengni Kebiwo, Alice Ninon Tsitsol Meke, Stéphane Talbit Ndjonya, Mesmin Dehayem Yefou, Eugène Sobngwi

“…The prevalence of diabetes in the Yaoundé Central Prison was 9.4%. The main cardiovascular risk factors were a sedentary lifestyle (91.1%), hypertension (39.6%), smoking (31.6%), and alcohol consumption (28.1%). …”
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Anaesthesia provision, infrastructure and resources in the Heilongjiang Province, China: a cross-sectional observational study by Jian Zhang, Hui Wang, Fei Han, Ge Jiang, Xi Zhang, Guonian Wang, Xiaoyu Zheng, Jingshun Zhao, Dandan Yao, Wanchao Yang, Xuesong Ma, Xiaodi Lu, Xidong Zhu, Meijun Chen, Mingyue Zhang

“…Objective The aim of this study was to explore the current status of the anaesthesia provision, infrastructure and resources in the Heilongjiang Province, China.Design A cross-sectional observational study of hospitals, anaesthesiologists, assistant anaesthesiologists and anaesthetic nurses in the Heilongjiang Province.Setting All hospitals in the Heilongjiang Province.Participants The hospitals, anaesthesiologists (attending physicians, associate chief physicians and chief physicians), assistant anaesthesiologists (licenced assistant physicians, resident physicians and other trainees) and anaesthetic nurses.Main outcome measures Standard descriptive statistics (percentages and numbers) were used to summarise the data.Results The investigation involved 1123 hospitals, 405 of these hospitals had anaesthesiology departments (36.06%). …”
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IMMUnity Unveiled: A Translational NETwork for tackling PARKinson's Disease – IMMUPARKNET [version 2; peer review: 2 approved] by Tamara Saksida, Alessia di Flora, Marina Romero-Ramos, Vladimirs Pilipenko, Laura Muñoz Delgado, Franca Marino, Yasemin Gursoy Ozdemir, Neda Nikolovski, Cristoforo Comi, Dale Lawson, Inês Figueira, Mitilda Gugu, Simona Poletti, Shubhra Acharya, Dogukan Pira

“…Although widely recognized, the role of immunity in the onset and development of PD is still unclear. The main goal of IMMUPARKNET is to fill this knowledge gap by establishing an innovative, interdisciplinary research network and fostering exchanges of expertise among specialists from different countries and institutions. …”
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