RET inhibition overcomes resistance to combined CDK4/6 inhibitor and endocrine therapy in ER+ breast cancer by Charlotte K. Kindt, Sidse Ehmsen, Sidse Ehmsen, Sidse Ehmsen, Sofie Traynor, Benedetta Policastro, Nikoline Nissen, Mie K. Jakobsen, Monique F. Hundebøl, Lene E. Johansen, Martin Bak, Elsa Arbajian, Johan Staaf, Henrik J. Ditzel, Henrik J. Ditzel, Henrik J. Ditzel, Carla L. Alves

“…The clinical relevance of RET expression in ER+ breast cancer was investigated by gene array analysis of primary tumors treated with endocrine therapy and by immunohistochemical scoring of metastatic lesions from patients who received combined CDK4/6i and endocrine therapy.ResultsWe show that RET is upregulated in ER+ breast cancer cell lines resistant to combined CDK4/6i and fulvestrant compared to isogenic cells resistant to fulvestrant alone. siRNA-mediated silence of RET in high RET-expressing, combined CDK4/6i- and fulvestrant-resistant cells reduced their growth partially by affecting cell cycle regulators of the G2-M phase and E2F targets. Notably, targeting RET with selpercatinib in combination with CDK4/6i inhibited the growth of CDK4/6i-resistant cell lines and resensitized ER+ breast cancer patient-derived organoids resistant to CDK4/6i. …”
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A self-activated and protective module enhances the preclinical performance of allogeneic anti-CD70 CAR-T cells by Zhao Zhang, Lianfeng Zhao, Tinghui Huang, Zhengliang Chen, Zhengliang Chen, Yaoyao Zhao, Yaoyao Zhao, Junqing Liang, Xudong Ao, Xiaoqiong Jia, Lei Kang, Linghui Kong, Qi Jing, Jianhua Hu, Lili Gu, Feiyan Pan, Zhigang Hu, Lingfeng He, Muya Zhou, Jiannan Chen, Zhigang Guo

“…Anti-CD70 UCAR-T cells were then generated by knocking out TRAC, B2M, and HLA-DRA. To address the limitations of UCAR-T therapy, we developed an 'all-in-one' self-activated and protective (SAP) module, integrated into the CAR scaffold. …”
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Comparative evaluation of sensititre YeastOne and VITEK2 antifungal susceptibility tests with CLSI broth microdilution method of clinical Cryptococcus isolates in Taiwan by Tzu-Ping Weng, Shin-Wei Wang, Shih-Ting Lo, Shu-Li Su, Ming-I Hsieh, Pei-Jane Tsai, Pei-Fang Tsai, Chi-Jung Wu, Nan-Yao Lee, Wen-Chien Ko, Po-Lin Chen

“…Additionally, all C. neoformans isolates were susceptible to AMB with their MIC ≤1 µg/mL according to the clinical breakpoint defined by EUCAST. …”
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Maintenance of patient-reported health-related quality of life post neoadjuvant relugolix prior to the initiation of prostate radiation therapy by Min Jung Koh, Min Ji Koh, Jessica Y. Hsueh, Lindsey Gallagher, Malika Danner, Alan Zwart, Marilyn Ayoob, Deepak Kumar, Michael Carrasquilla, Paul Leger, Nancy A. Dawson, Simeng Suy, Sean P. Collins

“…Our investigation determined clinical significance based on minimally important difference (MID) calculated as 0.5 times the baseline standard deviation.ResultsAmong the 87 patients, average age was 71 years, 42% were non-white, and 24% were considered obese (BMI ≥30 kg/m²). Relugolix was initiated a median of 4 months before SBRT initiation (IQR: 3.9–5.4), with 87% of patients reaching profound castration (<20 ng/dl). …”
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Comparative evaluation of ACetic - MEthanol high salt dissociation approach for single-cell transcriptomics of frozen human tissues by Marina Utkina, Anastasia Shcherbakova, Ruslan Deviatiiarov, Ruslan Deviatiiarov, Ruslan Deviatiiarov, Alina Ryabova, Marina Loguinova, Valentin Trofimov, Anna Kuznetsova, Mikhail Petropavlovskiy, Rustam Salimkhanov, Denis Maksimov, Eugene Albert, Alexandra Golubeva, Walaa Asaad, Lilia Urusova, Ekaterina Bondarenko, Anastasia Lapshina, Alexandra Shutova, Dmitry Beltsevich, Oleg Gusev, Oleg Gusev, Oleg Gusev, Oleg Gusev, Larisa Dzeranova, Galina Melnichenko, Ildar Minniakhmetov, Ivan Dedov, Natalya Mokrysheva, Sergey Popov

“…This technique aims to preserve cell morphology and RNA integrity, minimizing transcriptome changes and providing a more accurate representation of mature mRNA. We compared the ability of enzymatic, ACME HS, and nuclei isolation methods to preserve major cell types, gene expression, and standard quality parameters across 41 tissue samples. …”
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Prevalence of chronic kidney disease among Chinese adults with diabetes: a nationwide population-based cross-sectional studyResearch in context by Weiping Jia, Rong Yu, Limin Wang, Dalong Zhu, Lixin Guo, Jianping Weng, Hong Li, Mei Zhang, Xiaoqi Ye, Zhiguang Zhou, Dajin Zou, Qiuhe Ji, Xiaohui Guo, Yinan Zhang, Dong Lang, Jiarui Wu, Jing Wu, Xuhong Hou, Xiaohui Guo, Kai Wu, Liming Chen, Demin Liu, Guangyao Song, Linyi Shu, Jing Yang, Yan Wang, Dongmei Li, Qiansha Guo, Ling Li, Na Wu, Yadong Sun, Huifang Cheng, Hongyu Kuang, Huijuan Zhang, Weiping Jia, Xuhong Hou, Dalong Zhu, Jian Zhu, Hong Li, Fenping Zheng, Qiu Zhang, Honglin Hu, Gang Chen, Xingquan Yang, Xiaoyang Lai, Jianping Liu, Li Chen, Ming Dong, Zhigang Zhao, Jian Liu, Guangda Xiang, Junxia Zhang, Zhiguang Zhou, Jian Peng, Jianping Weng, Longyi Zeng, Yuzhen Liang, Guoqiao Li, Kaining Chen, Leweihua Lin, Qifu Li, Qingfeng Cheng, Xingwu Ran, Dawei Chen, Hong Li, Xin Nian, Lihui Yang, Shuyou Meng, Jing Xu, Junhong Long, Jing Liu, Qi Zhang, Qingxiang Dai, Xiaomin Xie, Guirong Bai, Jun Li, Tao Li, Zhong Dong, Tao Zhang, Sijia Liu, Yong Yang, Kun Geng, Wenlong Zheng, Zhihong Li, Hui Wang, Zuojun Wu, Miao Wang, Jixin Sun, Jianwei Zhou, Jun An, Huaqing Shen, Yanfang Li, Zeping Ren, Xiuli Xue, Shaohui Jia, Yongping Zheng, Jiaoxia Li, Yonggang Qian, Ping Ma, Yan Su, Liang Cao, Xuyi Yang, Guowei Pan, Guangzhi Pan, Ling Jin, Quanfu Yu, Yunfei Liu, Yingli Zhu, Zhifang Cheng, Jun Yang, Chundi Jia, Qing Wang, Shichun Yan, Jingyang Huang, Yumei Chi, Li Zhang, Ruifa Pang, Yan Shi, Yan Lu, Yiling Wu, Jie Yu, Chunxiang Wu, Jinyi Zhou, Yunxia Wu, Ning Zhang, Jing Sun, Xiumei Tian, Jieming Zhong, Jingying Chen, Kaixu Xie, Lingjuan Fu, Xiaohua Wang, Yeji Chen, Ling Zeng, Zhenqian Cao, Maomin Yang, Biao Hu, Wenling Zhong, Qingyan Liu, Zhiding Huang, Qingbin Lai, Yuyu Zhang, Liping Zhu, Yujing Zhao, Wuming Tao, Yonghai Tu, Lin Zhou, Xiaolei Guo, Benzheng Chai, Liping Ding, Yuewei Zou, Dongzhi Li, Li Hua, Shixian Feng, Jianli Guo, Wanguang Liu, Qingxiang Li, Yunzhi Zheng, Minli Xu, Lan Zhang, Chunbo Li, Yingchao Wan, Chi Hu, Shengping Xu, Biyun Chen, Huilin Liu, Xiaojia Bian, Wenwei Chen, Honghua Li, Yanjun Xu, Fan Weng, Hong Wu, Weijin Zhang, Liankai Zhu, Jun Meng, Jin Yang, Yongsong Zeng, Gaoke Zhai, Lingfeng Qin, Yi Lu, Zhenwang Fu, Shukuan Wu, Caigang Li, Na Wang, Lianfen Chen, Xianbin Ding, Qi Zhou, Yiling Xie, Rui Chen, Dongsheng Yang, Ying Deng, Youping Hu, Bo Huang, Ping Wang, Peng Cai, Changyan Peng, Xiaofang Chen, Ling Li, Liang Xiao, Chenglin Wang, Chaogang Tan, Daobin Chen, Min Chen, Mingfang Qin, Zhaomin Lei, Jianzhi Qiu, Wei Yu, Yunfei Li, Guoxia Bai, Ying Wang, Lin Qiu, Ning Wang, Xudong Zhao, Ani Yan, Jianfei Liu, Tingcai Wang, Kaihua Hao, Zaihong Zhang, Zhanqi Xian, Tenghuan Ma, Zhihua Xu, Yi Yang, Hongli Wang, Rong Li, Zhonggang Zhao, Caixia Fan, Purhati, Hongjun Xu, Liping Gao

“…CKD was identified by the presence of albuminuria (urine albumin-to-creatinine ratio ≥30 mg/g) and/or decreased estimated glomerular filtration rate (eGFR, <60 mL/min/1.73 m2). The latter was calculated using the CKD-EPI equation incorporating serum cystatin C and creatinine. …”
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Should the Food and Drug Administration Limit Placebo-Controlled Trials? by Max Goodman, Connor Pedersen

“… [2] Keränen T, Halkoaho A, Itkonen E, Pietilä A-M. Placebo-controlled clinical trials: How trial documents justify the use of randomisation and Placebo. …”
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High level non-carbapenemase carbapenem resistance by overlaying mutations of mexR, oprD, and ftsI in Pseudomonas aeruginosa by Yan Yang, Xue Li, Lang Sun, Xiu-Kun Wang, You-Wen Zhang, Jing Pang, Guo-Qing Li, Xin-Xin Hu, Tong-Ying Nie, Xin-Yi Yang, Jian-Hua Liu, Gerrit Brandis, Xue-Fu You, Cong-Ran Li

“…The OprD (Trp415*) truncation affected porin structure, and the constructed mutant Pae d1 Green oprD Trp415* increased meropenem MIC by 16-fold (from 0.25 to 4 µg/mL). The contribution of ftsI mutation to meropenem resistance was confirmed by clinical linkage analysis and was estimated to cause a two-fold increase in meropenem MIC by comparing the resistant clinical isolate with the Pae d1 Green oprD Trp415*∆mexR double mutant. …”
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Spirituality as predictor of psychological well-being at work in the Moroccan context: A cross-sectional study by Mohamed MAKKAOUI, Fatima-Zahra HANNOUN, Khalid OUAZIZI, Amelia RIZZO, Driss AIT ALI, Abdelhalim CHERQUI, Kaoutar KHABBACHE, Murat YILDIRIM, Mahmood BAHRAMIZADEH, Sefa BULUT, Łukasz SZARPAK, Michal PRUC, Kavita BATRA, Gabriella NUCERA, Francesco CHIRICO, Hicham KHABBACHE

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EZH1/2抑制剂UNC1999对外周血免疫细胞表型的影响 by 刘丹平, 池沛冬, 梅美华, 游莹, 黄俊琪

“…结果相比对照组，UNC1999组经典型单核细胞（CD14⁺⁺CD16^-）比例上调［（19.53±1.79）% <italic>vs</italic>. （66.60±5.02）%，<italic>t</italic>=13.31，<italic>P</italic>=0.006］，中间型单核细胞（CD14⁺⁺CD16⁺）和非经典型单核细胞（CD14⁺CD16⁺）比例下调［（35.08±3.97）% <italic>vs</italic>. （15.42±2.89）%，<italic>t</italic>=6.130，<italic>P</italic>=0.026；（35.50±3.53）% <italic>vs</italic>. （8.40±3.12）%，<italic>t</italic>=25.740，<italic>P</italic>=0.002］； CD56^dimCD16⁺、CD56^domCD16⁺ NK细胞亚群比例下调［（3.39±0.86）% <italic>vs</italic>. （0.27±0.06）% ，<italic>t</italic>=4.882，<italic>P</italic>=0.040；（80.50±0.64）% <italic>vs</italic>. （0.63±0.23）% ，<italic>t</italic>=133.100，<italic>P</italic>&lt;0.000 1］；初始B细胞比例上调［（10.67±1.76）% <italic>vs</italic>. （37.99±3.76）%，<italic>t</italic>=17.690，<italic>P</italic>=0.003］，记忆B细胞、过渡B细胞、浆细胞比例下调［（23.39±4.20）% <italic>vs</italic>. （11.82±1.90）%，<italic>t</italic>=7.059，<italic>P</italic>=0.020；（3.58±0.47）% <italic>vs</italic>. （1.52±0.56）%，<italic>t</italic>=26.970，<italic>P</italic>=0.001；（0.18±0.03）% <italic>vs</italic>. （0.00±0.00）%，<italic>t</italic>=8.647，<italic>P</italic>=0.013］；DC比例上调［（0.20±0.05）% <italic>vs</italic>. （ 1.38±0.13）%，<italic>t</italic>=16.500，<italic>P</italic>=0.004］，其中pDC/DC下调［（24.90±1.95）% <italic>vs</italic>. （12.70±2.11）%，<italic>t</italic>=7.566，<italic>P</italic>=0.017］，mDC/DC上调［（32.41±13.14）% <italic>vs</italic>. （60.87±8.43）%，<italic>t</italic>=8.252，<italic>P</italic>=0.014］；CD8⁺T细胞亚群中CD8⁺中枢记忆T细胞、CD8⁺PD-1⁺比例上调［（5.62±1.24）% <italic>vs</italic>. （18.38±2.34）%，<italic>t</italic>=15.600，<italic>P</italic>=0.004 ；（2.50±1.02）% <italic>vs</italic>. （18.34±2.69）% ，<italic>t</italic>=8.822，<italic>P</italic>=0.013］，CD8⁺效应记忆T细胞比例下调［（28.27±10.15）% <italic>vs</italic>. （15.62±9.48）% ，<italic>t</italic>=19.480，<italic>P</italic>=0.003］；CD4⁺T细胞亚群中CD4⁺CD27⁺比例下调［（82.77±2.66）% <italic>vs</italic>. （56.00±9.01）%，<italic>t</italic>=5.715，<italic>P</italic>=0.029］。…”
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Grain Yield Stability Analysis of Lentil Genotypes by AMMI Indices by Payam Pezeshkpour, Reza Amiri, Iraj Karami, Amir Mirzaei

“…In the field, each plot consisted of 4 m planting rows with a distance of 25 cm and a density of 200 seeds per square meter. …”
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Intervention effect and mechanism of Dracocephalum moldavica L. extract on bleomycin-induced pulmonary fibrosis in rats by Xiaoyu SUN, Li CHEN, Ruifang GAO, Hui YU, Min JIN

“…Real-time fluorescence quantitative PCR (RT-qPCR) was used to detect α-SMA and Col-I mRNA levels in lung tissue. Enzyme-linked immunosorbent assay (ELISA) was used to detect tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in rats. …”
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Макроморфологічні та мікроморфологічні дослідження ґрунтів Консулівського городища (І ст. до н. е. – ІІ ст. н. е.)... by Жанна Матвіїшина, Анатолій Кушнір, Надія Гаврилюк, Матера Марчин

“…The modern soil in the interval of 0.0–0.8 m corresponds to the characteristics of low-humus southern chernozems formed on a light loamy substrate. …”
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Prolonged venous transit is associated with worse neurological recovery in successfully reperfused large vessel strokes by Janet Mei, Hamza Adel Salim, Dhairya A. Lakhani, Licia Luna, Aneri Balar, Mona Shahriari, Nathan Z. Hyson, Francis Deng, Adam A. Dmytriw, Adrien Guenego, Vaibhav Vagal, Victor C. Urrutia, Elisabeth B. Marsh, Hanzhang Lu, Risheng Xu, Rich Leigh, Dylan Wolman, Gaurang Shah, Benjamin Pulli, Kambiz Nael, Gregory W. Albers, Max Wintermark, Jeremy J. Heit, Tobias D. Faizy, Argye E. Hillis, Raf Llinas, Vivek Yedavalli

“…After adjusting for age, sex, hypertension, diabetes, atrial fibrillation, administration of intravenous thrombolysis (IVT), Alberta Stroke Program Early CT Scores (ASPECTS), mTICI 2c and/or 3, Tmax >6 s volume, and hemorrhagic transformation, PVT+ was significantly associated with lower NIHSS percent change (B = −0.163, 95%CI −0.326 to −0.001, p = 0.049) and was less likely to achieve higher than 70% NIHSS improvement (OR = 0.331, 95% CI 0.127–0.863, p = 0.024). …”
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Neoandrographolide inhibits mature osteoclast differentiation to alleviate bone loss and treat osteoporosis by Kai Tang, Kai Tang, Kai Tang, Wei Deng, Wei Deng, Wei Deng, Wei Deng, Zhiying Huang, Zhiying Huang, Zhiying Huang, Simin Chen, Simin Chen, Simin Chen, Zilin Zhu, Zilin Zhu, Shukun Lin, Shukun Lin, Lubin Zhong, Lubin Zhong, Quanxin Zheng, Quanxin Zheng, Wenhua Zhao, Wenhua Zhao, Zhida Zhang, Zhida Zhang, Ling Mo, Ling Mo, Ling Mo

“…Finally, we performed in vivo experiments to further investigate the inflammation.ResultsNEO exhibits no significant cytotoxic effects on BMMs at concentrations less than or equal to 30 μM while exerting inhibitory effects on OC differentiation during its early and middle stages. …”
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Genetic characteristics involving the PD-1/PD-L1/L2 and CD73/A2aR axes and the immunosuppressive microenvironment in DLBCL by Tingting Zhang, Zhenzhen Zhang, Xiubao Ren, Jin He, Zhengzi Qian, Bin Meng, Lei Jiao, Hengqi Liu, Lanfang Li, Lihua Qiu, Shiyong Zhou, Wenchen Gong, Huilai Zhang, Xianhuo Wang

“…CD73 genetic mutations did not increase mRNA and protein expression. Patients with genetically altered CD73 tended to have a better overall survival than patients with wild-type CD73. …”
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Geohazard features of the Gulf of Naples and Pontine Islands (Eastern Tyrrhenian Sea) by Francesca Budillon, Eleonora Martorelli, Alessandro Conforti, Giovanni De Falco, Alessandro Bosman, Gabriella Di Martino, Marco Firetto Carlino, Martina Misuraca, Sara Innangi, Martina Pierdomenico, Andrea Sposato, Renato Tonielli, Daniele Casalbore, Roberto Capperucci, Aida Maria Conte, Francesco G. Falese, Michela Ingrassia, Ennio Marsella, Cristian Montanaro, Eleonora Morelli, Roberto Sisinni, Francesco L. Chiocci

“…The surveyed area encompasses a recently formed continental margin, which connects the internal segments of the Apennine fold-and-thrust belt, verging NE, to the Tyrrhenian Sea bathyal plain, a 3000-m-deep back-arc basin that has developed since the Middle-Late Miocene. …”
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A Path to the Stars: The Evolution of the Species by Fabrizio Vitali, Guido Chincarini, Mario Zannoni, Stefano Covino, Emilio Molinari, Stefano Benetti, Carlotta Bonoli, Favio Bortoletto, Enrico Cascone, Rosario Cosentino, Francesco D'Alessio, Paolo D'Avanzo, Vincenzo De Caprio, Massimo Della Valle, Alberto Fernandez-Soto, Dino Fugazza, Enrico Giro, Demetrio Magrin, Giuseppe Malaspina, Lech Mankiewicz, Raffaella Margutti, Ruben Mazzoleni, Luciano Nicastro, Alberto Riva, Marco Riva, Ruben Salvaterra, Paolo Spanò, Monica Sperandio, Mauro Stefanon, Gino Tosti, Vincenzo Testa

“…Then, we proposed to study the feasibility of a 4 m fast pointing class telescope, equipped with a multichannel imagers, from Visible to Near Infrared. …”
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The Effect of Adding Surfactants on Appearance Specification and Nutrients Content of Processed Corn Grain using Steam-Flaked Technique by Farzaneh Mohamady, Mohsen Danesh Mesgaran, Seyed Alireza Vakili, Abdolmansour Tahmasebi, Mohammadreza Hosseindokht

“…The ethanolic extract of Laurus nobilis (Ln) was provided by dissolving 100 g of dried and ground Ln in 500 mL of 96% v/v ethanol/water and shaking for 72 hours, then, the plant extract was prepared using evaporating method. …”
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Solid Predominant Histology and High Podoplanin Expression in Cancer-Associated Fibroblast Predict Primary Resistance to Osimertinib in EGFR-Mutated Lung Adenocarcinoma by Yuji Uehara, MD, Hiroki Izumi, MD, PhD, Tetsuro Taki, MD, PhD, Tetsuya Sakai, MD, PhD, Hibiki Udagawa, MD, PhD, Eri Sugiyama, MD, PhD, Shigeki Umemura, MD, PhD, Yoshitaka Zenke, MD, PhD, Shingo Matsumoto, MD, PhD, Kiyotaka Yoh, MD, PhD, Shoko Kubota, MD, PhD, Keiju Aokage, MD, PhD, Naoya Sakamoto, MD, PhD, Shingo Sakashita, MD, PhD, Motohiro Kojima, MD, PhD, Michiko Nagamine, MD, PhD, Yukio Hosomi, MD, PhD, Masahiro Tsuboi, MD, PhD, Koichi Goto, MD, PhD, Genichiro Ishii, MD, PhD

“…We also investigated HGF mRNA expression levels, using The Cancer Genome Atlas Program and Singapore Oncology Data Portal cohorts. …”
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