PGC-1α辅助激活人PEPCK基因转录

“…【目的】构建人磷酸烯醇式丙酮酸羧激酶(PEPCK)基因启动子荧光素酶报告质粒PGL3-hPCK-luc，并探讨过氧化物酶体增殖物活化受体γ辅助激活因子1α(PGC-1α)在转录因子肝细胞核因子4α(HNF4α)介导下对人PEPCK基因启动子活性的影响【方法】从人全血基因组DNA中克隆PEPCK启动子基因片段，并重组进荧光素酶报告载体PGL3-basic，将pcDNA3.1-PGC-1αpcDNA3.0-HNF4α表达质粒与PGL3-hPCK-luc按不同组合共转染进人肝癌细胞株HepG2细胞或正常人肝细胞株LO2细胞,培养48 h后检测细胞裂解液中荧光素酶活性【结果】通过酶切鉴定及测序证明扩增的人PEPCK启动子片段成功插入载体质粒中转染后人PEPCK启动子基因荧光素酶活性较空白对照组增加60倍(P < 0.05)；共转染质粒进HepG2细胞或LO2细胞后，HNF4α均可以增强人PEPCK启动子基因荧光素酶活性，在HepG2细胞，荧光素酶活性是对照组的2.69倍(P < 0.05)，而在LO2细胞中，荧光素酶活性是对照组的3.64倍(P < 0.05)；PGC-1α可以明显增强HNF4α对人PEPCK启动子的激活作用，在HepG2细胞，荧光素酶活性是PGL3-HPCK-luc+HNF4α组的2.01倍(P < 0.05)，而在LO2细胞中,荧光素酶活性是PGL3-HPCK-luc+HNF4α组的2.82倍(P < 0.05)而与单一转染组相比，共转染pcDNA3.1-PGC-1α与pcDNA3.0-HNF4α显著增加了PEPCK的mRNA和蛋白表达水平(P < 0.05)【结论】人PEPCK启动子基因报告质粒成功构建，且转录因子HNF4α可以激活人PEPCK启动子活性，辅助激活因子PGC-1α可以进一步加强HNF4α的作用…”
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Efficacy and Safety of Non-Vitamin K Anticoagulants for Atrial Fibrillation in Relation to Different Renal Function Levels: A Network Meta-Analysis by Hao Jin, Kongbo Zhu, Lina Wang, Yifan Li, Jingjun Meng, Hong Zhi

“…And it does not coincide with patients taking dabigatran 110 mg with dose reduction for other factors including aged ≥75 years, renal impairment (CrCL 30–50 mL/min), gastritis, esophagitis, or gastroesophageal reflux, receiving concomitant verapamil, and so on. …”
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Dietary Micronutrient Intake and the Prevalence of Metabolic Conditions among Children from the United States–Affiliated Pacific Region in the Children’s Healthy Living Program by Lucia A Seale, Ashley B Yamanaka, Kristi Hammond, Eunjung Lim, Lynne R Wilkens, Pauline McFall, Tanisha F Aflague, Patricia Coleman, Travis Fleming, Leslie Shallcross, Jonathan Deenik, Rachel Novotny

“…Objectives: The study examined the association between micronutrient intake with body mass index (BMI in kg/m2) and the presence of insulin resistance proxy, acanthosis nigricans (AN), in 3529 children aged 2–8 y from the USAP region in the Children’s Healthy Living study. …”
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Early-life famine exposure may modify the association between long-term temperature variability and cardio-cerebrovascular diseases: a nationwide study by Yao Wu, BinBin Su, Junqing Xie, Bo Wen, Xiaolan Wu, Mengfan Wang, Yanhui Dong, Yi Song, Jun Ma

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Corrigendum: Organizational readiness for change towards implementing a sepsis survivor hospital to home transition-in-care protocol by Elaine Sang, Elaine Sang, Ryan Quinn, Michael A. Stawnychy, Michael A. Stawnychy, Michael A. Stawnychy, Michael A. Stawnychy, Jiyoun Song, Jiyoun Song, Jiyoun Song, Karen B. Hirschman, Karen B. Hirschman, Karen B. Hirschman, Sang Bin You, Sang Bin You, Katherine S. Pitcher, Katherine S. Pitcher, Nancy A. Hodgson, Nancy A. Hodgson, Nancy A. Hodgson, Patrik Garren, Melissa O'Connor, Sungho Oh, Sungho Oh, Kathryn H. Bowles, Kathryn H. Bowles, Kathryn H. Bowles, Kathryn H. Bowles

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Effect of sun dry brewer spent yeast on chemical composition, in vitro digestibility, and ruminal degradation kinetics of wheat straw by Geberemariyam Terefe, Mulugeta Walelgne, Dereje Fekadu, Getu Kitaw, Mesfin Dejene, Aeimro Kehaliu, Bethlehem Mekonnen, Yohannse Habteyesus

“…The sample (3 g and 2 mm sieve size) with nylon bags (6.5 X 14 cm and 50 μm pore size) was entered sequentially and manually pressed deep into the liquid phase of the ventral sac of the rumen and incubated in the rumens (6, 12, 24, 48, 72, and 96 h) of three fistulated Boran × Holstein–Friesian steers. …”
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Alterations in DNA Damage Repair Genes Before and After Neoadjuvant Cisplatin-based Chemotherapy in Muscle-invasive Bladder Cancer by Ursula Lemberger, Büsra Ernhofer, Sigurd Krieger, Andreas Bruchbacher, André Oszwald, Ekaterina Laukhtina, Andrea Haitl, Melanie R. Hassler, Bernhard Englinger, Eva Compérat, Shahrokh F. Shariat

“…Key findings and limitations: Twenty-five patients (54%) had a response (<pT2 pN0 cM0) to NAC. Responders had more somatic DDR gene variants in preNAC (53 vs 11; p < 0.001) and postNAC (51 vs 9; p = 0.038) tumor tissue in comparison to nonresponders, as well as significantly greater phosphorylation of H2AX after NAC. …”
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The control effect and induced disease resistance mechanism of Bacillus tequilensis on wheat powdery mildew by Qiuyan Bi, Fen Lu, Jie Wu, Xiangyu Liu, Xiuying Han, Jianjiang Zhao

“…The results revealed that B. tequilensis controlled WPM at an effective concentration of 1 × 107 cfu/mL or higher. The inhibitory effect in vitro was 90.91–100.00 %. …”
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A new approach for evaluating regional permafrost changes: A case study in the Hoh Xil on the interior Qinghai‒Tibet Plateau by Yu-Xin Zhang, Lin Zhao, Chang-Wei Xie, Guo-Jie Hu, Shu-Hua Yang, De-Fu Zou, Yong-Ping Qiao, Xiang-Fei Li, Jia-Jie Peng

“…The simulated ground temperature profiles in four boreholes from various permafrost zones revealed an error of less than 0.7 °C below 5 m in depth. Model validation demonstrates the reliability of this approach for predicting long-term permafrost changes. …”
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3-Hydroxyanthranic acid inhibits growth of oral squamous carcinoma cells through growth arrest and DNA damage inducible alpha by Guifang Gan, Xinxia Zhou, Qiaoping Zheng, Xianfu Gao, Xu Chen, Han Zhang, Jinghao Liu, Zhaopeng Shi, Fuxiang Chen

“…Exogenous 3-HAA hampered growth of OSCC cells both in vitro and in vivo. 3-HAA induced growth arrest, G2/M-phase arrest, and apoptosis of OSCC cells. RNA-seq indicated that 3-HAA significantly increased GADD45A expression. 3-HAA promoted transcription of GADD45A by transcription factor YY1. …”
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Liposome-like encapsulation of fish oil-based self-nano emulsifying formulation for improved bioavailability by Iqbal Ahmad, Aparana Dogra, Tanya Nagpal, Chandvi Sharma, Sudhakar Singh, Nikhil Shaiva, Gaurav Saini, Kanishk Luhach

“…The optimized formulation exhibited significantly smaller particle sizes (100–300 nm), low PDI (0.149–0.303), and a stable zeta potential(-32.5 to -27.9 mV), potentially contributing to improved absorption and stability. …”
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Study of the hereditary non-syndromic ophthalmological pathology of child population of the Karachay-Cherkess Republic: estimations of genetic load and molecular genetic analysis by V.V. Kadyshev, A.V. Marakhonov, S.I. Kutsev, R.A. Zinchenko

“…Zinchenko^1,3<br> ¹ Research Centre of Medical Genetics of the Russian Academy of Medical Sciences, Moscow,&nbsp;Russian Federation<br> ² Pirogov Russian National Research Medical University, Moscow, Russian Federation<br> ³ M. F. Vladimirsky Moscow Regional Research and Clinical Institute (MONIKI), Moscow,&nbsp;Russian Federation<br> <br> <b>Abstract</b><br> The publication continues the previously presented data on the study of the epidemiology of ophthalmic diseases in the children of the Karachay-Cherkess Republic. …”
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Antioxidant effect of Lactobacillus fermentum HFY02-fermented soy milk on D-galactose-induced aging mouse model by Tiantian Hu, Rui Chen, Yu Qian, Ke Ye, Xingyao Long, Kun-Young Park, Xin Zhao

“…The activities of GSH, GSH-Px, SOD, CAT and T-AOC in serum, brain and liver of soybean milk fermented by LF-HFY02 were significantly increased, while the content of MDA and the level of AGEs in hippocampal were significantly decreased compared with D-galactose induced group. Further more, the mRNA expression of GSH and SOD in mouse liver were obviously up-regulated by soybean milk fermented by LF-HFY02. …”
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Ear Infection and Its Associated Risk Factors in First Nations and Rural School-Aged Canadian Children by Chandima P. Karunanayake, William Albritton, Donna C. Rennie, Joshua A. Lawson, Laura McCallum, P. Jenny Gardipy, Jeremy Seeseequasis, Arnold Naytowhow, Louise Hagel, Kathleen McMullin, Vivian Ramsden, Sylvia Abonyi, Jo-Ann Episkenew, James A. Dosman, Punam Pahwa, The First Nations Lung Health Project Research Team, The Saskatchewan Rural Health Study Team

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Ｄｌｌ４和ＶＥＧＦＲ在氧诱导视网膜病小鼠模型中的表达及意义

“…两组各取产后７ ｄ（７ ｄ ｐｏｓｔ－ｐａｒｔｕｍ，ｐ７）、ｐ１２、ｐ１７新生鼠 ５只，摘除眼球，取视网膜提取ＲＮＡ，以ＲＴ－ＰＣＲ 方法检测Ｄｌｌ４、 ＶＥＧＦＲ－１、 ＶＥＧＦＲ－２在视网膜中ｍＲＮＡ的表达。【结果】 ＲＴ－ＰＣＲ结果显示，在ｐ７和ｐ１２时间点时，两组间ＶＥＧＦＲ－１表达差异无统计学意义（Ｐ ＞ ０．０５）；在ｐ１７时间点时，两组间ＶＥＧＦＲ－１表达差异有统计学意义（Ｐ ＜ ０．０５），实验组表达量低于对照组；ＶＥＧＦＲ－２在各时间位点两组间表达差异无统计学意义（Ｐ ＞ ０．０５）；在ｐ７时间点时，两组间Ｄｌｌ４表达差异无统计学意义（Ｐ ＞ ０．０５）；在ｐ１２和ｐ１７时间点时，两组间Ｄｌｌ４表达差异有统计学意义（Ｐ ＜ ０．０５，Ｐ ＜ ０．０５），实验组表达量低于对照组；实验组不同时间点ＶＥＧＦＲ－１ 、Ｄｌｌ４的表达，随着时间的延长而下降（Ｐ ＜ ０．０５， Ｐ ＜ ０．０５）；ＶＥＧＦＲ－２的表达，随着时间的延长而升高（Ｐ ＜ ０．０５）；对照组不同时间点的ＶＥＧＦＲ－２表达随着时间延长而增加（Ｐ ＜ ０．０５），而ＶＥＧＦＲ－１和Ｄｌｌ４的表达均不随时间变化而变化（Ｐ ＞ ０．０５，Ｐ ＞ ０．０５）；Ｄｌｌ４的表达与ＶＥＧＦＲ－１的表达呈正相关关系，相关系数ｒ ＝ ０．９０５，Ｐ ＜ ０．００１；而Ｄｌｌ４与ＶＥＧＦＲ－２、ＶＥＧＦＲ－１与ＶＥＧＦＲ－２之间不存在相关关系。…”
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学龄儿童眼球后极部脉络膜厚度及相关因素分析 by 王丹阳, 谭倩, 秦嘉敏, 郭惠宇, 矫操, 张亮, 钟兴武

“…【结果】7~16岁学龄儿童脉络膜厚度在黄斑中心凹下为（SF）250（204~314）μm，以黄斑中心凹为中心向鼻侧和下方变薄，向颞侧和上方变厚，最薄点位于鼻侧3mm处，最厚位于颞侧1mm处，随近视度数增加，黄斑区各测量点脉络膜厚度均有不同程度变薄，且黄斑中心凹及周围1mm处脉络膜变薄较周边更加显著（成组秩和检验，P<0.05）。…”
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Effects of Metformin Added to Insulin in Adolescents with Type 1 Diabetes: An Exploratory Crossover Randomized Trial by Daizhi Yang, Jinhua Yan, Hongrong Deng, Xubin Yang, Sihui Luo, Xueying Zheng, Jing Lv, Wen Liang, Mengjie Hong, Zekai Wu, Bin Yao, Jianping Weng, Wen Xu

“…Seventeen patients with mean±SD age 14.4±2.3 years, body mass index 18.17±1.81 kg/m2, median (IQR) diabetes duration 4.50 (3.58, 6.92) years, and HbA1c 9.0% (8.5%, 9.4%) were enrolled. …”
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Optimization of osmotic pressure swelling method in the process of hemoglobin extraction from red blood cells by ZHANG Honghui, ZHOU Wentao, HAO Shasha, WANG Hong, LIU Jiaxin, YANG Chengmin, LI Shen, LI Fengjuan

“…[Results] The optimal matching conditions for preparing red blood cell swelling solution were obtained through orthogonal experiment as follows: osmotic pressure molar concentration was 30 mOsmol/Kg, pH was 7.8, and phosphate buffer to red blood cell volume ratio was 6∶1. …”
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Ethanol extract of Polygonatum cyrtonema Hua mitigates non-alcoholic steatohepatitis in mice by Dongliang Chen, Yue Shen, Yue Shen, Fang Huang, Bo Huang, Shangfu Xu, Shangfu Xu, Lisheng Li, Lisheng Li, Jie Liu, Zheng Li, Xia Li

“…PCE restored the expression of AMPK, SIRT1, SREBP1 and PPAR-α both in mRNA and protein levels. RNAseq identified unique gene expression profiles in response to high-fat diet (HFD) compared to the PCE treatments. …”
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Dynamic development of gut microbiota and metabolism during and after weaning of kittens by Hong Zhang, Yang Ren, Siyu Wei, Hongli Jin, Yizhen Wang, Mingliang Jin

“…Results Significant changes in the immune response and gut microbiota were observed in kittens following weaning. Specifically, IgM levels increased significantly (P < 0.01, n = 20), while IgA and IgG levels showed a sustained elevation. …”
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