中华草龟源维氏气单胞菌的分离及生物学特性鉴定 by 赵允清 邵明珠 郭家媚 葛 成 周云宵吴同垒 张志强 任 海 张召兴 张艳英

“…结果显示:CRQ1株对斑马鱼的半数致死量( LD₅₀)为3.57×10⁷cfu/mL,携带<i>aerA、act、aha、Ser、Exu、Lip、Hly、ompA</i>和<i>LuxS</i> 9种毒力基因; 分离菌对替米考星、环丙沙星和恩诺沙星等9种药物敏感,对阿米卡星、链霉素和磺胺间甲氧嘧啶等8种药物中度敏感,对新斯的明耐药; 该分离菌携带四环素类耐药基因<i>tet(A)、tet(E),</i>氟喹诺酮类耐药基因<i>oqxA、gyrA、gyrB、parC、parE,</i>氨基糖苷类耐药基因<i>aac</i>2<i>、aac(</i>6<i>)-Ib、aadB、aac</i>4<i>,</i>磺胺类耐药基因<i>sul-</i>1<i>,</i>大环内酯类耐药基因<i>ermB</i>,分离菌含有多类抗菌药物的耐药基因,但未表达出相应的耐药表型,这与用药时间长短和用药频率有关。…”
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Dynamics of retinal changes in early-stage Parkinson’s disease by Ane Murueta-Goyena, Sara Teijeira-Portas, Elisa Blanco Martín, Raquel Vázquez-Picón, Blanca Ruiz Bajo, Jone Bocos, Jorge Sánchez-Molina, Patricia Alves Dias, Ioana Croitoru, Iñaki Rodríguez Agirretxe, Rocío Del Pino, Marian Acera, Beatriz Tijero, Oihane Sáez-Atxukarro, David Romero-Bascones, Juan Carlos Gómez-Esteban, Javier Aritz Urcola, Javier Ruiz Martínez, Iñigo Gabilondo

“…In PD patients, the baseline GCIPL thickness in central regions was increased by up to 4 μm, and the rate of thinning in the parafoveal GCIPL was − 0.61 [0.29] µm/year faster over a one-year follow-up period than in controls in the 2- to 3-mm ring (p = 0.039). …”
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Identification of standing dead trees in Robinia pseudoacacia plantations across China’s Loess Plateau using multiple deep learning models by Li Zhang, Xiaodong Gao, Shuyi Zhou, Zhibo Zhang, Tianjie Zhao, Yaohui Cai, Xining Zhao

“…As expected, the YOLOv9-ECA model demonstrated significant advancements, achieving a detection speed of 123.5f/s, a mAP of 97.8%, and an F1 score of 0.97, outperforming other models in both detection efficiency and accuracy. …”
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Advanced approach to primary microsurgical debridement of extensive scleral lacerations involving simultaneous vitrectomy and chorioretinal wound closure by S.S. Shamkin, S.N. Subbotina, A.B. Stepanyants, D.V. Salov

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CDK14 regulates the development and repair of lung by Jian-wei Chen, Yu-xiang Wang, Rong-rong Gao, Lan-yue Ma, Jing Zhong, Jia-xin Yang, Zhao-hua Deng, Yu-yan Li, Xiao-ling Li, Ya-hai Shu, Wen-jing Guo, Zi-yuan Zhou, Xiao Yu Tian, Jinjin Ma, Yang Liu, Qi Chen

“…Mechanistically, Cdk14 ablation inhibited the proliferation of epithelial and vascular endothelial cells, inducing cell cycle arrest at the G2/M phase. Through RNA-seq analysis of both endothelial and epithelial cells, we found that knockdown of Cdk14 controls the expression of signal transducers and activator of transcription 1 (STAT1) as well as associated genes in interferon signaling. …”
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Dual-immunomodulatory effects on RAW264.7 macrophages and structural elucidation of a polysaccharide isolated from fermentation broth of Paecilomyces hepiali by Baoming Tian, Renjian Liu, Qi Li, Hanshan Xu, Ming Cai, Rongfa Guan, Peilong Sun, Kai Yang

“…The nitric oxide (NO) production, phagocytosis activity, and cytokine secretion were significantly increased (P < 0.05) after PHP40-2 treatment; however, PHP40-2 could inhibit NO production and cytokine secretion in LPS-induced RAW264.7 macrophages (P < 0.05). After mRNA and protein expression analysis, toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway was discovered to play an important role in the dual-immunomodulatory effects of PHP40-2. …”
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Association of ERAP1 and ERAP2 gene polymorphisms and ERAP2 protein with the susceptibility and severity of rheumatoid arthritis in the Ukrainian population by Iryna Kril, Iryna Kril, Andrzej Wiśniewski, Agnieszka Tarnowska, Khrystyna Lishchuk-Yakymovych, Yaryna Bojko, Piotr Kuśnierczyk, Valentyna V. Chopyak, Izabela Nowak

“…ERAP2 concentration above 5.85 ng/mL indicated a severe phase of RA.ConclusionsSome ERAP1 and ERAP2 polymorphisms seem to be related to susceptibility to RA or the severity of the disease. …”
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Synthesis of Fe3O4@MIL-101-OH/Chitosan for adsorption and release of doxorubicin by Iman Najafipour, Nafiseh Emami, Pegah Sadeh, Adonis Amoli, Sareh Mosleh-Shirazi, Ali Mohammad Amani, Hesam Kamyab, Shreeshivadasan Chelliapan, Seyed Reza Kasaee, Ehsan Vafa

“…Also, this nanocomposite has a high specific surface area (610.36 m2/g). In this article, the effect of pH, contact time, and drug concentration on DOX adsorption were investigated, and the results showed that at optimal conditions, pH = 8, contact time = 90 min, and drug concentration is 350 ppm, the adsorption capacity of MIL-101-OH/Chitosan and Fe3O4@MIL-101-OH/Chitosan are equal to 185 and 174.3 mg/g. …”
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Sex-differences in the risk of carpal tunnel syndrome: results from a large Ontario, Canada worker cohort by Fanni R. Eros, Paul A. Demers, Jeavana Sritharan

“…Results A total of 3,224 CTS cases among females (f) and 2,992 cases among males (m) were identified in the cohort. We observed elevated risks of CTS in many occupations requiring repetitive and forceful manual work: slaughtering and meat cutting, canning, curing and packing (HRf = 2.28, 95%CI = 1.42–3.68; HRm = 1.95, 95%CI = 1.33–2.85); welding and flame cutting (HRf = 1.83, 95%CI = 1.01–3.32; HRm = 1.65, 95%CI = 1.35–2.01); motor vehicle fabricating and assembling (HRf = 2.14, 95%CI = 1.74–2.62; HRm = 2.43, 95%CI = 2.04–2.89); and packaging (HRf = 2.06, 95%CI = 1.43–2.97; HRm = 1.82, 95%CI = 1.12–2.98). …”
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Behaviors of Water Intake, Hydration Status, and Related Hydration Biomarkers among Physically Active Male Young Adults in Beijing, China: A Cross-Sectional Study by Na Zhang, Jianfen Zhang, Xing Wang, Yibin Li, Yi Yan, Guansheng Ma

“…The median daily total water intake (TWI), TDF, and WFF were 2701, ik1789, and 955 mL, respectively. Among participants, 17 participants (16%) were in euhydration status, 47 participants (43%) were in hypohydration, and 45 participants (41%) were in middle hydration. …”
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Effects of Anthocyanin and Flavanol Compounds on Lipid Metabolism and Adipose Tissue Associated Systemic Inflammation in Diet-Induced Obesity by Roel A. van der Heijden, Martine C. Morrison, Fareeba Sheedfar, Petra Mulder, Marijke Schreurs, Pascal P. H. Hommelberg, Marten H. Hofker, Casper Schalkwijk, Robert Kleemann, Uwe J. F. Tietge, Debby P. Y. Koonen, Peter Heeringa

“…Furthermore, HFD+B, but not HFD+E, significantly prevented the early upregulation of circulating neutrophil chemoattractant mKC. However, no differences in AT histopathology were observed between the HFD types. …”
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Transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female Jilin white geese by Jin Yu, Ichraf Mabrouk, Qiuyuan Liu, Yuxuan Zhou, Yupu Song, Jingyun Ma, Fengshuo Liu, Xiangman Hu, Zhiyi Yang, Yuxia Zeng, Jingtao Hu, Yongfeng Sun

“…These DEGs were enriched in Gene Ontology (GO) terms related to the microtubule cytoskeleton, cytoskeleton, cell adhesion, and biological adhesion and the KEGG enrichment analysis suggested that the DEGs were mainly enriched in “MAPK signaling pathway”, “Wnt signaling pathway”, “Calcium signaling pathway”, “Focal adhesion”, “Neuroactive ligand-receptor interaction”, “Melanogenesis”, “mTOR signaling pathway”, “ECM-receptor interaction”, and “TGF-beta signaling pathway” in the two comparison groups. …”
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Association between sex steroid hormones and α-klotho: Results from the NHANES 2013-2016 and Mendelian randomization study by Andong Guo, Pengcheng Chen, Jishuang Cao, Chenrui Wu, Sentai Ding

“…Results: In men, significant positive correlations with α-Klotho were consistent across both unadjusted and fully adjusted models for total testosterone (TT), bio-available testosterone (Bio-T), estradiol (E2) and sex hormone-binding globulin (SHBG) (Model 3: TT: β = 3.54, 95 % CI: 1.63–5.44, P = 0.0003; Bio-T: β = 1.74, 95 % CI: 0.73–2.74, P = 0.0007; E2: β = 0.25, 95 % CI: 0.11–0.38, P = 0.0003; SHBG: β = 0.95, 95 % CI: 0.63–1.27, P < 0.0001); In premenopausal women, we detected a potential nonlinear relationship between TT levels and α-Klotho, with α-Klotho levels rising to a peak at a TT level of 72.2 ng/mL, after which they declined. Furthermore, results from MR analyses reaffirmed positive associations of TT and Bio-T with α-Klotho in men (TT: β = 3.54, 95 % CI: 1.63–5.44, P = 0.0003; Bio-T: β = 1.74, 95 % CI: 0.73–2.74, P = 0.0007). …”
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Mast Cells Positive to Tryptase and c-Kit Receptor Expressing Cells Correlates with Angiogenesis in Gastric Cancer Patients Surgically Treated by Michele Ammendola, Rosario Sacco, Giuseppe Sammarco, Giuseppe Donato, Valeria Zuccalà, Roberto Romano, Maria Luposella, Rosa Patruno, Carlo Vallicelli, Giorgio Maria Verdecchia, Davide Cavaliere, Severino Montemurro, Girolamo Ranieri

“…In a series of 25 gastric cancer patients with stage T3N2-3M0 (by AJCC for Gastric Cancer 7th Edition), immunohistochemistry and image analysis methods were employed to evaluate in the tumour tissue the correlation between the number of mast cells positive to tryptase (MCPT), c-KitR expressing cells (c-KitR-EC), and microvascular density (MVD). …”
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Integrating single-cell RNA-seq and bulk RNA-seq to explore prognostic value and immune landscapes of methionine metabolism-related signature in breast cancer by Yanxian Gao, Ziyu Feng, Hailong Zhao, Xinghai Liu, Muyu Zhu, Xiafei Yu, Xiaoan Liu, Xian Wu, Jing Tao

“…Subsequent in vitro experiments demonstrated that silencing APOC1 reduced the generation of tumor-associated macrophages (TAMs) with an M2 phenotype while significantly decreasing the proliferation, invasion, and migration of MDA-MB-231 and MDA-MB-468 breast cancer cell lines.ConclusionWe established a prognostic risk score consisting of genes associated with methionine metabolism, which helps predict prognosis and response to treatment in BC. …”
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Machine vision-based detection of key traits in shiitake mushroom caps by Jiuxiao Zhao, Jiuxiao Zhao, Wengang Zheng, Wengang Zheng, Yibo Wei, Yibo Wei, Qian Zhao, Qian Zhao, Jing Dong, Jing Dong, Xin Zhang, Xin Zhang, Mingfei Wang, Mingfei Wang

“…After conducting correlation analysis between phenotypic features and shiitake mushroom caps weight, four most correlated phenotypic features were identified: Area, Perimeter, External rectangular width, and Long axis; they were divided into four groups based on their correlation rankings. Finally,M3 group using GWO_SVM algorithm achieved optimal performance among six mainstream machine learning models tested with an R²value of 0.97 and RMSE only at 0.038 when comparing predicted values with true values. …”
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Development of a prognostic nomogram and risk stratification system for elderly patients with esophageal squamous cell carcinoma undergoing definitive radiotherapy: a multicenter r... by Yuanji Xu, Chuyan Lin, Chun Han, Xin Wang, Yidian Zhao, Qingsong Pang, Xinchen Sun, Gaofeng Li, Kaixian Zhang, Ling Li, Xueying Qiao, Yu Lin, Zefen Xiao, Junqiang Chen

“…Results Significant prognostic factors identified included diabetes, tumor volume (GTVp), tumor length, location, and clinical stages (T, N, M), and RT response. Multivariate analysis confirmed these as independent factors for OS. …”
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Cecum microbiota composition, fermentation characteristics, and immunometabolic biomarkers of Yunshang black goat fed varying dietary energy and protein levels by Binlong Fu, Xiaoqi Zhao, Muhammad Khan, Yanting Jiang, Weijuan Li, Maida Mushtaq, Baiji Danzeng, Xiaojun Ni, Zobia Azeem, Qingyong Shao, Bai Xue, Yina Ouyang

“…VFA metabolism, absorption, cytokine expression, and tight junction protein mRNA in cecal tissue were also analyzed. Genes like MCT-1 and SLC16A4, linked to VFA absorption, positively correlated with Paludibacter, which was associated with immune markers (TLR-3, TLR-4, IFN-γ) and Occludin expression. …”
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Longitudinal genetic studies of cognitive characteristics by R. N. Mustafin, A. V. Kazantseva, R. F. Enikeeva, S. B. Malykh, E. K. Khusnutdinova

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人 工 皮 Bio Fill®的 实 验 研 究

“…结果:①BF 组 诸兔术后能饮水、进食,次晨即恢复活泼可爱状态; 生理盐水组精神状态差,有 2 兔术后 20 h 内死亡,其余各兔术后 3 d 才恢复正常活动;②实 验组兔伤后第 1、2、3、6 天的基础代谢比对照组低(P <0. 05);③实验组兔伤后第 1、2、3、6 天血清丙二醛含量较对照组低(P <0. 05);④两组 兔的生化 8 项变化无显著差异;⑤液体接种法的10 个菌种培养后全部有细菌生长, BF 无抑菌或杀菌能力; 平板接种法的结果相同,但 BF 下面 培养基上无细菌生长,或者已生长的菌落缩小、消失,在BF 上面接种的细菌不能穿过 BF,于其下面培养基上生长;⑥用 BF 覆盖的创面,光镜 下见 BF 呈均质红染膜状,其下有薄层的坏死组织,再下为正常的肉芽组织,无排斥反应;⑦电镜见 BF 的纵切面厚约 50～ 100 μ m, 由多层弯曲 的纤维组成,纤维间有小而弯曲的窦道样间隙,有微孔通向 BF 的表面。结论: 用 BF 覆盖创面的兔病理生理改变比对照组轻,恢复快; BF 不能 抑菌或杀菌,但能防止细菌从外界侵入创面; 用 BF 覆盖的培养基不利于细胞生长; 应用 BF 后无排斥现象。…”
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