Alternative splicing in the DBD linker region of p63 modulates binding to DNA and iASPP in vitro by Rebecca Lotz, Christian Osterburg, Apirat Chaikuad, Sabrina Weber, Masato Akutsu, Anne Christin Machel, Ulrike Beyer, Jakob Gebel, Frank Löhr, Stefan Knapp, Matthias Dobbelstein, Xin Lu, Volker Dötsch

“…Moreover, the concomitant loss of the charged amino acids in the alternatively spliced version decreases the affinity of p63 to its cognate DNA element two- to threefold. mRNAs encoding full-length p63, as well as its alternatively spliced version, are present in all tissues that we investigated, albeit in differing ratios. …”
Get full text

CAIL: Cross-Modal Vehicle Reidentification in Aerial Images Using the Centroid-Aligned Implicit Learning Network by Haoran Gao, Yiming Yan, Yanming He, Jianzheng Zhou, Zhengning Zhang, Yunchao Yang

“…Our proposed approach achieves significant and satisfactory performance on a cross-modal aerial images dataset, in terms of both mAP and rank-1 accuracy.…”
Get full text

Intensive critical care and management of asthmatic and smoker patients in COVID-19 infection by Lu Dongming, Yaqoob Obaid, Kumar Manish, Kushwah Ajay Singh, Sharma Rahul Kumar, Kumar Devinder, Mavai Yogendra, Khan Rukaiya

Get full text

Mini-cutting technique for the propagation of a Eucalyptus camaldulensis clone selected in a semiarid region by Francisco Emerson F. de Felice, Poliana C. Dias-Araujo, Ewerton S. Pinheiro, Carlos Vergara

“…Main results: The mini-stumps from E. camaldulensis clone showed 100% survival, 13 propagules per mini-stump/month and 900 sprouts/m2/month. The adventitious rooting was lower in control (55%) than in IAA (85%) and IBA (80%) at 2.000 mg.L-1. …”
Get full text

Direct Observations of North Pacific Subsurface Low Potential Vorticity Water Impinging on the Kuroshio by Ran Wang, Qiang Ren, Feng Nan, Fei Yu, Zifei Chen, Yansong Liu, Jianfeng Wang, Chuanzheng Zhang, Ruixiang Zhao, Hua Zheng, Xiaohua Zhu

“…In addition, the SLPVW impingement results in a significant subsurface velocity variation (about 10 cm/s) in the 200–400 m layer. These findings shed new light on the mechanisms of the intraseasonal variation in the Kuroshio subsurface layer.…”
Get full text

Single-cell transcriptomics of bronchoalveolar lavage during PRRSV infection with different virulence by Byeonghwi Lim, Seung-Chai Kim, Hwan-Ju Kim, Jae-Hwan Kim, Young-Jun Seo, Chiwoong Lim, Yejee Park, Sunirmal Sheet, Dahye Kim, Do-Hwan Lim, Kyeongsoon Park, Kyung-Tai Lee, Won-Il Kim, Jun-Mo Kim

“…Furthermore, anti-inflammatory M2-like macrophages (SPP1-CXCL14high) are identified and their counts increase during the peak lung-damage time point, likely contributing to local defense and lung recovery, which is not observed in high virulent infection. …”
Get full text

Assessment of Confidence Levels of Undergraduate Senior Dental Students and Interns’ During Endodontic Treatment Procedures in a Sample From Saudi Arabia by Madfa AA, Almansour MI, Al‑Zubaidi SM, Almohefer SA, Alajlan SS, Alsaleh MK, Alanazi YA, Alajlan AS

“…Ahmed A Madfa,1 Moazzy I Almansour,1 Saad M Al‑Zubaidi,1 Sami A Almohefer,1 Sarah S Alajlan,1 Mohammed K Alsaleh,1 Yosef A Alanazi,2 Abdalellah S Alajlan2 1Department of Restorative Dental Science, College of Dentistry, University of Ha’il, Ha’il, Kingdom of Saudi Arabia; 2Dental Student, College of Dentistry, University of Ha’il, Ha’il, Kingdom of Saudi ArabiaCorrespondence: Ahmed A Madfa, Department of Restorative Dental Science, College of Dentistry, University of Hail, Hail, Kingdom of Saudi Arabia, Tel +966165358200, Email ahmed_um_2011@yahoo.comBackground: The evaluation of students’ impression regarding their dental college experience is a crucial indicator of the effectiveness and achievement of dental education. …”
Get full text

HUMAN DESTRUCTIVENESS IN THE EXISTING PRACTICES OF LATE MODERNISM VIOLENCE: POSITIVE AND NEGATIVE DIMENSIONS by O. V. Marchenko, L. V. Martseniuk

“…Originality. Based on the works of M. Scheler and E. Fromm defining for philosophical anthropology and psychoanalysis, for the first time the conceptualization of positive and negative forms of manifestation of human destructiveness against the background of sociocultural transformations of late Modernism was carried out. …”
Get full text

干扰素-α/β体外增敏替莫唑胺对MGMT阳性胶质瘤干细胞作用

“…对MGMT阳性胶质瘤干细胞生长抑制实验显示,干扰素-α/β作用后提高了替莫唑胺的化疗敏感性,杀伤效应显著增强;RT-PCR、Western-blot检测结果表明,干扰素-α/β作用后,MGMT阳性胶质瘤干细胞NF-κB、MGMT 在mRNA及蛋白水平表达均明显降低。【结论】 对于MGMT阳性的胶质瘤干细胞,干扰素-α/β能够显著增加替莫唑胺的抗肿瘤效应,其机制可能是干扰素-α/β干预后,下调NF-KB的表达,从而降低了MGMT的转录表达,逆转替莫唑胺的化疗耐药。…”
Get full text

A Chisel Generator for Standardized 3-D Die-to-Die Interconnects by Harrison Liew, Farhana Sheikh, Jong-Ru Guo, Zuoguo Wu, Borivoje Nikolic

“…This work concludes with the results of a redundancy design space exploration tradeoff study, showing the benefits of a proposed spatial coding redundancy scheme in an example PHY using emulated 9-<inline-formula> <tex-math notation="LaTeX">$\mu $ </tex-math></inline-formula>m hybrid bonding for a 4 Tx/4 Rx module array with 4:1 coding redundancy ratio.…”
Get full text

β-巯基乙醇和bFGF分别诱导大鼠骨髓基质干细胞 成神经元样细胞的生物学性状

“…【目的】 比较β-巯基乙醇(BME)和bFGF分别诱导大鼠骨髓基质干细胞(MSC)所分化神经元样细胞的生物学性状65377; 【方法】 BME和bFGF分别作用于大鼠的MSC,分别于9 h和5 d后,进行神经元样细胞的细胞计数,然后用免疫荧光进行神经元特异性蛋白NF200的鉴定,RT-PCR的方法分析神经元特异性基因NF的表达;Western blotting对检测神经元特异性蛋白NF200的表达65377;【结果】 BME诱导的细胞在加入药物1 h后形态发生改变,9 h时有突起伸出,胞体变亮; bFGF诱导的细胞在诱导后第3天开始有突起伸出,呈神经元样,第5天突起之间相互连接,第7天胞体死亡;而两种药物所分化的神经元样细胞NF200的免疫荧光均成阳性,但bFGF诱导的神经元样细胞的阳性率要显著高于BME所诱导的神经元样细胞; 在RT-PCR中,bFGF诱导的神经元样细胞神经元特异性基因NF200的表达要高于BME所诱导的细胞; western blotting中,bFGF诱导的神经元样细胞神经元特异性蛋白NF200的表达要高于BME所诱导的细胞65377; 【结论】 bFGF和BME均可诱导MSC分化为神经元样细胞65377;但bFGF所诱导的神经元样细胞在从形态上更接近于神经元,而神经元特异性基因NF200 mRNA及神经元特异性蛋白NF200蛋白的表达,均高于BME所诱导的细胞65377;…”
Get full text

The Influence of Monoamine Oxidase Variants on the Risk of Betel Quid-Associated Oral and Pharyngeal Cancer by Ping-Ho Chen, Bin Huang, Tien-Yu Shieh, Yan-Hsiung Wang, Yuk-Kwan Chen, Ju-Hui Wu, Jhen-Hao Huang, Chun-Chia Chen, Ka-Wo Lee

“…A significant trend of MAO-A mRNA expression was found in in vitro studies. …”
Get full text

Polyp and tumor microenvironment reprogramming in colorectal cancer: insights from mucosal bacteriome and metabolite crosstalk by Hadi Feizi, Hossein Samadi Kafil, Andrey Plotnikov, Vladimir Kataev, Alexander Balkin, Ekaterina Filonchikova, Mohammad Ahangarzadeh Rezaee, Reza Ghotaslou, Mohammad Sadrkabir, Hiva Kadkhoda, Fadhil S. Kamounah, Sergei Nikitin

“…Conclusion These results proposed that beneficial bacteria including the Lachnospiraceae family depletion cross-talk with acetoacetate and BHB accumulations followed by an increased abundance of driver bacteria including G. morbillorum, and M. morganii may reprogram polyp microenvironment leading to tumor initiation. …”
Get full text

Integrated Bioinformatics-Based Identification of Potential Diagnostic Biomarkers Associated with Diabetic Foot Ulcer Development by Long Qian, Zhipeng Xia, Ming Zhang, Qiong Han, Die Hu, Sha Qi, Danmou Xing, Yan Chen, Xin Zhao

“…GO analyses revealed these DEGs to be significantly enriched in biological processes including sarcomere organization, muscle filament sliding, and the regulation of cardiac conduction, molecular functions including structural constituent of muscle, protein binding, and calcium ion binding, and cellular components including Z disc, myosin filament, and M band. These DEGs were also enriched in the adrenergic signaling in cardiomyoctes, dilated cardiomyopathy, and tight junction KEGG pathways. …”
Get full text

Safety and tumour-specific immunological responses of combined dendritic cell vaccination and anti-CD40 agonistic antibody treatment for patients with metastatic pancreatic cancer:... by Joachim G J V Aerts, Sjoerd H van der Burg, Martijn P Lolkema, Sai Ping Lau, Freek R van ’t Land, Marjolein Y V Homs, Casper H J van Eijck

“…Secondary endpoints include radiographic response according to the RECIST (V.1.1) and iRECIST criteria, and the detection of antitumour specific immune responses.Ethics and dissemination The Central Committee on Research Involving Human Subjects (CCMO; NL76592.000.21) and the Medical Ethics Committee (METC; MEC-2021-0566) of the Erasmus M.C. University Medical Center Rotterdam approved the conduct of the trial. …”
Get full text

Association between maternal gestation weight gain and preterm birth according to pre-pregnancy body mass index and HbA1c by Xiaoxiao Zhang, Binbin Yin, Kaiqi Wu, Lei Fang, Yan Chen

“…Among women with pp-BMI <18.5 kg/m2, women with inadequate GWG had a significantly increased risk of PTB compared with women in the control group (HbA1c 4.6–5.0%, appropriate GWG), and the risk increased with increasing HbA1c levels. …”
Get full text

Effects of cinnamon, rosemary and oregano on growth performance, blood biochemistry, liver enzyme activities, excreta microbiota and ileal morphology of Campylobacter jejuni‐challe... by Zahra Alimohammadi, Hassan Shirzadi, Kamran Taherpour, Enayat Rahmatnejad, Ali Khatibjoo

“…Except for the NC group, all chicks were orally challenged with 2 × 108 CFU/mL C. jejuni daily from days 21–25. Feed intake, body weight gain (BWG), feed conversion ratio (FCR), energy efficiency ratio (EER) and protein efficiency ratio (PER) were assessed during the rearing period (0–42 days). …”
Get full text

Utilizing Remote Sensing to Quantify Evapotranspiration: A Case Study of the Saiss Basin (Morocco) by Oumou Abdellah, Essahlaoui Ali, Khrabcha Abdelali, Essahlaoui Narjisse, Alitane Abdennabi, Kassou Amina, El Hafyani Mohammed, Van Rompaey Anton, Gobin Anne

“…Two satellite images were used at a resolution of 15m: one for January as a wet season and another for July as a dry period. …”
Get full text

Naked-Eye Molecular Testing for the Detection of <i>Xylella fastidiosa</i> in Mallorca (Balearic Island) Almond Orchards by Colorimetric LAMP by Amoia Serafina Serena, Ana Falcón-Piñeiro, Milica Pastar, José Manuel Garcìa-Madero, Nicoletta Contaldo, Mikael Muegge, Stéphane Compant, Pasquale Saldarelli, Angelantonio Minafra

“…The cLAMP assay was able to detect up to 100 CFU/mL of the <i>Xf</i> bacterial suspension diluted in healthy almond sap. …”
Get full text

Dipeptidyl Peptidase 4 Activity Is Related to Body Composition, Measures of Adiposity, and Insulin Resistance in Subjects with Excessive Adiposity and Different Degrees of Glucose... by Wellington S. Silva Júnior, Maria das Graças C. Souza, José Firmino Nogueira Neto, Eliete Bouskela, Luiz Guilherme Kraemer-Aguiar

“…First, we investigated the relationships of constitutive DPP4 activity, body composition (assessed by bioelectrical impedance analysis), and measures of adiposity and IR in fifty-two subjects of both sexes, 18-50 years, and BMI ≥25.0 kg/m2 who comprised three groups according to glucose tolerance. …”
Get full text