Miniaturization and characterization of patient derived hepatocellular carcinoma tumor organoid cultures for cancer drug discovery applications by David A. Close, Paul A. Johnston

“…Non-invasive sequentially acquired transmitted light images showed that seeding cryopreserved cells from non-tumoral and HCC PDTOs into 96- or 384-well plates in reduced growth factor Matrigel (rgf-MG) that were fed with growth medium every 3 days supported organoid growth up to 15 days. …”
Get full text

Structural Characterization of B-DNA d(CGTGAATTCACG)₂ in Complex with the Specific Minor Groove Binding Fluorescent Marker Hoechst 33342 by Hristina Sbirkova-Dimitrova, Rusi Rusew, Hristo Gerginov, Annie Heroux, Boris L. Shivachev

“…DAPI and Hoechst 33342 are fluorescent dyes that bind to the minor groove of DNA, fluorescing brightly in the blue region with an emission maximum at approximately 461 nm when excited by ultraviolet light (~350 nm). This work focuses on the binding interactions of Hoechst 33342 with the specific DNA sequence d(CGTGAATTCACG)₂. …”
Get full text

The CuePed Trial: How Does Environmental Complexity Impact Cue Effectiveness? A Comparison of Tonic and Phasic Visual Cueing in Simple and Complex Environments in a Parkinson’s Dis... by Rodney Marsh, Michael H. Cole, Nadeeka N. W. Dissanayaka, Tiffany R. Au, Sandra Clewett, John D. O’Sullivan, Peter A. Silburn

“…We assembled 20 participants with PD and FoG and familiarized them with a belt-worn, laser-light cueing device (Agilitas™). The device was designed with 2 cueing modalities—gait-dependent or “phasic” cueing and gait-independent or “tonic” cueing. …”
Get full text

Preparation and In Vivo Expression of CS-PEI/pCGRP Complex for Promoting Fracture Healing by Chun-Liang Li, Feng Qin, Rong-rong Li, Jia Zhuan, Hai-Yong Zhu, Yu Wang, Kai Wang

“…The particle size and zeta potential of CS-PEI/pCGRP nanoparticles were detected by dynamic light scattering. The binding of CS-PEI to pCGRP was detected by gel retardation assay. …”
Get full text

Redo Aortic Valve Replacement After Prior Transcatheter versus Surgical Aortic Valve Replacement: Insights from Western Experience by Arber Aliu, Klodiana Durici, Ira Lazo, Altin Veshti

“…This trend underscores the need for this informative article, which aims to provide insights into the risks and outcomes of aortic valve reoperations, particularly in light of efforts by interventional cardiologists to expand the indications for TAVR to younger and lower-risk patients. …”
Get full text

Synergistic effects of PGPRs and fertilizer amendments on improving the yield and productivity of Canola (Brassica napus L.) by Haji Muhammad, Muhammad Ijaz, Abdul Sattar, Sami Ul-Allah, Ahmad Sher, Muhammad Asif, Muhammad Dilshad, Khalid Mahmood, Muhammad Waheed Riaz, Muhammad Saqlain Zaheer, Muhammad Rizwan, Salim Manoharadas

“…Additionally, the application of Bacillus subtilis with biochar at 2 tons/ha improved the yield and quality of canola, as well as the structure and nutrient regulation of the soil. Conclusion In light of these results, we recommend the application of Bacillus subtilis to canola seeds along with either 2 t/ha biochar or the entire recommended dose of N: P:K (140:55:40 kg/ha). …”
Get full text

Improving remote sensing dehazing quality through local hybrid correction and optimization of atmospheric attenuation model based on wavelength by Daihong Zhao, Kun Shi, Zheng Li, Zheng Li, Meixiang Chen

“…Additionally, we refined the transmittance attenuation model using light reflection at the 550 nm wavelength from verdant landscapes, which improved the model’s alignment with real-world conditions. …”
Get full text

H3K4 methylation regulates development, DNA repair, and virulence in Mucorales by Macario Osorio-Concepción, Carlos Lax, Damaris Lorenzo-Gutiérrez, José Tomás Cánovas-Márquez, Ghizlane Tahiri, Eusebio Navarro, Ulrike Binder, Francisco Esteban Nicolás, Victoriano Garre

“…Additionally, set1 null strains were more sensitive to SDS, EMS, and UV light, indicating severe impairment in the repair process of the cell wall and DNA lesions and a correlation between Set1 and these processes. …”
Get full text

Prosocial competency-based model of a future teacher by E. I. Eroshenkova, I. S. Shapovalova, E. A. Karabutova, S. V. Anokhina, O. S. Miroshnikova

“…Such pedagogical competencies as “subject proficiency” and “humanism, respect for the individual, humanity” are among the leading ones. In the light of priorities within the competency-based model of a future teacher, the flexibility of the specific university training programmes is also revealed. …”
Get full text

Three-Dimensional Digital Documentation for the Conservation of the Prambanan Temple Cluster Using Guided Multi-Sensor Techniques by Anindya Sricandra Prasidya, Irwan Gumilar, Irwan Meilano, Ikaputra Ikaputra, Rochmad Muryamto, Erlyna Nour Arrofiqoh

“…First, APDs were established from the literature to derive the associated multiple detail levels; then, multiple sensors and modes of light detection and ranging (Lidar) scanners and photogrammetry were utilized according to their detail requirements and, finally, point cloud data were processed, integrated, assessed, and validated by the proof of the existence of an APD. …”
Get full text

Mass gatherings in Italy: a study from the 2015 Milan Expo by Pietro Marino, Enzo Albergoni, Aida Andreassi, Gianluca Chiodini, Lucia Colombi, Cristina Corbetta, Gabriella Nucera, Marco Salmoiraghi, Alberto Zoli

“…Our data indicate that 13,579 visitors were rescued in the ‘Red Area’ from 1 May to 31 October (with an average of 73 cases per day); 9,501 of them needed initial treatment or observation time at the First Aid Points, 1,289 of them were hospitalised (1% Red code, 29% Yellow code, 70% Green code); 65% of patients (57% female, with a mean age of 37 years old) had medical problems. Fatigue, light- headedness, dizziness, syncope, loss of consciousness and headache were the prevalent medical diagnoses. …”
Get full text

A systematic review of the transformation of Lake Beseka from brine to nearly fresh lake water by Yosef Abebe, Taye Alemayehu, Bizuneh Asfaw Abebe, Tena Alamirew, Beteley Teckola, Esayas Alemayehu

“…Large-scale water quality parameters demonstrate rapid alterations brought on by freshwater discharge into lakes, which may shed light on their use for livestock and crop production. …”
Get full text

A phenomenological qualitative study based on the multi-theory model (MTM) of health behavior change to identify factors and ways to design interventions for quitting gambling amon... by Laurencia BONSU, Sidath KAPUKOTUWA, Anita CHATTERJEE, Asma AWAN, Manoj SHARMA

“…Similarly, the sustenance phase is investigated, shedding light on the mechanisms that contribute to the continuation of gambling quitting behaviors over time. …”
Get full text

MORPHOFUNCTIONAL CHARACTERISTICS OF LEAVES AND FRUITS IN MALOIDEAE (ROSACEAE): b. THE ROLE OF SURFACE TISSUES IN THE FORMATION OF RESISTANCE TO FUNGAL DISEASES by T. Kh. Kumachova, O. O. Beloshapkina, A. S. Voronkov, A. S. Ryabchenko

“…However, in this work we combined the methods of light, electron (SEM, TEM) and confocal microscopy. …”
Get full text

Effect of substrate temperature and stages duration on recycling of agro-industrial residues through Pleurotus ostreatus production by Sami Abou Fayssal, Milena Yordanova, Youssef Sassine

“…This study also sheds light on the correlation between selected parameters, yield, and substrate composition.Method The experiment consisted of 2 sole substrates wheat straw (WS; control) and SCG, and 6 mixtures of WS, OLPR, and SCG (v/v): WS:SCG 3:1, WS:SCG 1:3, WS:OLPR:SCG 1:1:1, WS:OLPR:SCG 3:1:1, WS:OLPR:SCG 1:3:1, and WS:OLPR:SCG 1:1:3.Results Increased SCG proportions caused the substrate temperature to decrease, whereas, OPLR proportions caused it to rise. …”
Get full text

The Importance of Sleep in Athletic Performance - A Systematic Review by Julia Białeta, Katarzyna Rowińska, Karolina Siembab, Michalina Jurkiewicz, Agnieszka Napieralska, Wiktor Garbarczyk, Albert Kapla, Alicja Černohorská, Wiktoria Pysiewicz, Daria Bednarczyk

“…Evidence reveals that sleep interventions such as prolonging sleep duration, napping during the day and blue-enriched light exposure lead to improving sleep in athletes7. …”
Get full text

Calves gastrointestinal nematodes and Eimeria prevalence and associated risk factors in dairy farms, southern Ethiopia by Maireg Hailu, Kassahun Asmare, Endrias Zewdu Gebremedhin, Vincenzo Di Marco Lo Presti, Maria Vitale, Desie Sheferaw

“…Based on their morphological appearance, Strongyle type (20.3%), Trichuris (4.2%) and Ascaris (16.3%) eggs, Eimeria oocysts (21.5%) were observed. In the light of this finding, the authors would like to advise the need for strategic intervention. …”
Get full text

Influence of Gd alloying and ultrasonic vibration hybrid treatment on the microstructures, mechanical properties and strengthening mechanisms of Al/Mg interface by compound casting by Qingqing Li, Wenming Jiang, Yuancai Xu, Linghui Yu, Ziwei Peng, Yunchang Xin, Zitian Fan

Get full text

Modulation of the Microstructure and Enhancement of the Photocatalytic Performance of g-C3N4 by Thermal Exfoliation by Xinshan Zhao, Junwei Yu, Tingyu Meng, Yuanyuan Luo, Yanzhen Fu, Zhao Li, Lin Tian, Limei Sun, Jing Li

“…The analysis conducted using XRD, FT-IR, XPS, SEM, and elements mapping tests, illustrated an increase in nitrogen-vacancy and oxygen content on the surface of the CN photocatalyst, resulting in a porous and sparse structure, changes in crystal size, and improved visible light absorption performance. The photocatalytic reduction experiments of hexavalent chromium (Cr(VI)) showed that the CN-540 showed the highest reduction rate of 96.9%, with a reaction rate constant 6.21 times that of bulk-g-C3N4. …”
Get full text

Development and comprehensive greenness assessment of HPLC method for quality control of β-sitosterol in pharmaceutical ointments with trio-color coded evaluation by Haya I. Aljohar, Abdullah M. Al-Hossaini, Seham A. Alzammay, Samiah Alhabardi, Hadir M. Maher, Aya R. Ahmed

“…Additionally, the separation was achieved in a remarkably short analysis time of just 2.1 min, which not only enhanced throughput but also significantly minimized waste and solvent consumption, thereby making it a more sustainable and effective alternative for β-sitosterol extraction. Moreover, in the light of green and white analytical chemistry, a comprehensive ecological and sustainable tri-color coded assessment protocol was established. …”
Get full text