Comprehensive genome-scale CRISPR knockout screening of CHO cells by Sung Wook Shin, Su Hyun Kim, Aghiles Gasselin, Gyun Min Lee, Jae Seong Lee

“…In the present study, we conducted a genome-scale pooled CRISPR knockout (KO) screening using a virus-free, recombinase-mediated cassette exchange-based platform in CHO-K1 host and CHO-K1 derived recombinant cells. …”
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Effect of Global ATGL Knockout on Murine Fasting Glucose Kinetics by Margarida Coelho, Patricia Nunes, Vera M. Mendes, Bruno Manadas, Arend Heerschap, John G. Jones

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C-Reactive Protein Knockout Attenuates Temporomandibular Joint Inflammation in Rats by Yao He, Mengjiao Zhou, Zixiang Jian, Lingli Fang, Lan Huang, Jinlin Song

“…Freund’s complete adjuvant (CFA) was used to induce TMJ inflammation in CRP-knockout (CRP-/-) and control rats. Degenerative changes in the TMJ were compared to elucidate the role of CRP in TMJ inflammation. …”
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Gut Microbiome and Inflammation: A Study of Diabetic Inflammasome-Knockout Mice by Roma Pahwa, Miriam Balderas, Ishwarlal Jialal, Xinpu Chen, Ruth Ann Luna, Sridevi Devaraj

“…Gut microbiome composition and biomarkers of inflammation (IL-18, serum amyloid A) were assessed in streptozotocin- (STZ-) induced diabetic mice on a NLRP3-knockout (KO) background versus wild-type diabetic mice. …”
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Schistosoma japonicum Infection in Treg-Specific USP21 Knockout Mice by Youxiang Zhang, De-Hui Xiong, Yangyang Li, Guina Xu, Baoxin Zhang, Yang Liu, Shan Zhang, Qing Huang, Simin Chen, Fansheng Zeng, Jingyi Guo, Bin Li, Zhiqiang Qin, Zuping Zhang

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ARABIDOPSIS ATMKK1 KNOCKOUT MUTANT FLOWERS UNDER SHORT DAY CONDITIONS by Chad CONROY, Khadijah M. NIZAM, Tim XING

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Necroptotic Cell Death Signaling and Execution Pathway: Lessons from Knockout Mice by José Belizário, Luiz Vieira-Cordeiro, Sylvia Enns

“…Studies on multiple double- and triple- knockout mice identified caspase-8, flip, and fadd genes as key regulators of embryonic lethality and inflammation. …”
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Impaired Healing of a Cutaneous Wound in an Inducible Nitric Oxide Synthase-Knockout Mouse by Takashi Kitano, Hiroshi Yamada, Maki Kida, Yuka Okada, Shizuya Saika, Munehito Yoshida

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Knockout of <i>dhx38</i> Causes Inner Ear Developmental Defects in Zebrafish by Mengmeng Ren, Xiang Chen, Liyan Dai, Jiayi Tu, Hualei Hu, Xiaohan Sun, Jiong Luo, Pei Li, Yiyang Fu, Yuejie Zhu, Weiqiang Sun, Zhaohui Tang, Mugen Liu, Xiang Ren, Qunwei Lu

“…Here, we utilized a <i>dhx38</i> knockout zebrafish model to monitor the ear morphology and elucidate a crucial role for DHX38 in the development of the zebrafish inner ear. …”
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Capsaicin-Mediated Neurogenic Vasodilatation in Neurokinin-1, NK1, Receptor Knockout Mice by A. D. Grant, S. D. Brain

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Effect of Zbtb33 gene knockout and bacterial lipopolysaccharide on home cage behavior in mice by N. V. Khotskin, I. E. Sorokin, E. A. Kulikova, A. V.  Kulikov

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Estrogens and Spermiogenesis: New Insights from Type 1 Cannabinoid Receptor Knockout Mice by Giovanna Cacciola, Teresa Chioccarelli, Silvia Fasano, Riccardo Pierantoni, Gilda Cobellis

“…In this review, we summarize data which fuel this debate, with a particular attention to our recent results, obtained using type 1 cannabinoid receptor knockout male mice as animal model.…”
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Impaired cognitive function and decreased monoamine neurotransmitters in the DNAJC12 gene knockout mouse model by Shunan Wang, Ming Shen, Bo Pang, Bo Zhou, Yuan Yuan, Mei Lu, Xiangling Deng, Min Yang, Shufang Liu, Qiong Wang, Mei Xue, Qisheng Xia, Zhixin Zhang

Subjects: “…DNAJC12 gene knockout mice…”
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Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract by Hiroyuki Tanaka, Osamu Morinaga, Takuhiro Uto, Shunsuke Fuji, Frederick Asare Aboagye, Nguyen Huu Tung, Xiao Wei Li, Waraporn Putalun, Yukihiro Shoyama

“…The immunoaffinity concentration of glycyrrhizin was determined by immunoaffinity column conjugated with antiglycyrrhizin MAb resulting in the glycyrrhizin-knockout extract, which was determined by the synergic effect with glycyrrhizin on NO production using the cell line.…”
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Investigating the Impact of HTNV Infection on the Gut Microbiota of Wild-Type and NLRX1 Knockout Mice by Wenjie Sun, Yaxin Ding, Ziyu Liu, Shiyuan Hou, Danni Sun, Ruixue Ma, Huarui Kang, Xiaohan Ma, Jiayu Wang, Hongrui Mu, Yunhua Lv, Tianle Gu, Qikang Ying, Fang Wang, Xingan Wu, Rongrong Liu

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Fatty Liver and Insulin Resistance in the Liver-Specific Knockout Mice of Mitogen Inducible Gene-6 by Byung Kil Park, Eun-Ah Lee, Hee-Youn Kim, Jun Choul Lee, Koon Soon Kim, Won Hoon Jeong, Ki Young Kim, Bon Jeong Ku, Sang Dal Rhee

“…Mitogen inducible gene-6 (Mig-6) is a feedback inhibitor of epidermal growth factor receptor (EGFR) signaling pathway. The liver-specific knockout mice of the Mig-6 gene (Mig-6d/d) showed hepatomegaly and increased hypercholesterolemia. …”
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Compensatory synaptotagmin-11 expression conceals parkinson’s disease-like phenotypes in parkin knockout mice by Nan Dong, Zhenli Xie, Anqi Wei, Yuxin Yang, Yongning Deng, Xu Cheng, Bianbian Wang, Yang Chen, Yuhao Gu, Jingyu Yao, Yuhao Qin, Chaowen Zheng, Xi Zhang, Yuqing Zhang, Xinjiang Kang, Guoqing Chen, Qiumin Qu, Changhe Wang, Huadong Xu

“…Here, we provide compelling evidence that the compensatory expression of synaptotagmin-11 (Syt11) plays a key role in concealing PD-associated phenotypes in parkin knockout (KO) mouse models. Unlike the normal dopamine (DA) release and motor behaviors observed in parkin KO mice, parkin knockdown (KD) in the substantia nigra pars compacta (SNpc) in adult mice led to both the impaired DA release and the pronounced motor deficits. …”
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The mutation atlas of giant kelp (Macrocystis pyrifera): a mutation database resource for natural knockouts by Jose Francisco Diesel, Gary Molano, Sergey V. Nuzhdin

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<i>shp-2</i> gene knockout upregulates CAR-driven cytotoxicity of YT NK cells by V. G. Subrakova, S. V. Kulemzin, T. N. Belovezhets, A. N. Chikaev, N. A. Chikaev, O. A. Koval, A. A. Gorchakov, A. V. Taranin

“…We demonstrate that the combination of shp-2 gene knockout and CAR expression increases the cytotoxicity of effector NK-like YT cells against human prostate cancer cell line Du-145 with ectopic expression of PSMA protein, which is specifically targeted by the CAR.…”
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PD-L1 knockout or ZG16 overexpression inhibits PDAC progression and modulates TAM polarization by Hui Meng, Manman Nan, Yizhen Li, Yi Ding, Xiaokun Fang, Weiqian Ma, Mingzhi Zhang

Subjects: “…PD-L1 knockout…”
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