Point Mutations in the folP Gene Partly Explain Sulfonamide Resistance of Streptococcus mutans by W. Buwembo, S. Aery, C. M. Rwenyonyi, G. Swedberg, F. Kironde

“…In contrast, plasmids carrying four other mutations (A46V, E80K, Q122H, and S146G) in folP did not similarly confer any sulfonamide resistance to folP-knockout bacteria. Nevertheless, sulfonamide resistance (MIC = 50 μM) of folP-knockout bacteria transformed with plasmid-encoded folP was much less than the resistance (MIC = 4 mM) expressed by chromosomally-encoded folP. …”
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HDAC6 Facilitates PRV and VSV Infection by Inhibiting Type I Interferon Production by Hu Zheng, Xiaohui Yang, Haiwen Zhong, Changxu Song, Zhenfang Wu, Huaqiang Yang

“…Here, we investigated the role of HDAC6 in influencing viral infection in pig cells with the aim of exploiting the potential antiviral gene targets in pigs. Using gene knockout and overexpression strategies, we found that HDAC6 knockout greatly reduced PRV and VSV infectivity, whereas HDAC6 overexpression increased their infectivity in PK15 cells. …”
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Differential Expression of -Associated Genes in Autosomal Dominant Polycystic Kidney Disease by Yeon Joo Yook, Yu Mi Woo, Moon Hee Yang, Je Yeong Ko, Bo Hye Kim, Eun Ji Lee, Eun Sun Chang, Min Joo Lee, Sunyoung Lee, Jong Hoon Park

“…To address this question, we presently created Pkd2 knockout and PKD2 transgenic mouse embryo fibroblasts. …”
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Design of a resonant slow extraction from low-emittance electron booster rings using transverse resonance island buckets by E. C. Cortés García, I. V. Agapov, W. Hillert

“…In this contribution, we present the design of a resonant slow extraction based on the radio-frequency knockout (rf-KO) scheme, where we make use of the transverse resonance islands bucket (TRIB) optics. …”
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Zinc Supplementation Prevented Type 2 Diabetes-Induced Liver Injury Mediated by the Nrf2-MT Antioxidative Pathway by Lechu Yu, Yuanyuan Liu, Yichun Jin, Tinghao Liu, Wenhan Wang, Xuemian Lu, Chi Zhang

“…A high-fat diet, along with a one-time dose of streptozotocin, was applied to metallothionein (MT) knockout mice, nuclear factor-erythroid 2-related factor (Nrf) 2 knockout mice, and age-matched wild-type (WT) control mice, in order to induce type 2 diabetes. …”
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Piezo1 aggravates ischemia/reperfusion-induced acute kidney injury by Ca2+-dependent calpain/HIF-1α/Notch signaling by Xiaoting Chen, Jintao Jiang, Bin He, Shangfei Luo, Qiaorui Tan, Youfen Yao, Rentao Wan, Honglin Xu, Silin Liu, Xianmei Pan, Xin Chen, Jing Li

“…Genetically modified mice with targeted knockout of Piezo1 in myeloid cells were established, and acute kidney injury was induced by bilateral renal vascular clamping surgery. …”
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Muscarinic acetylcholine receptor 3 localized to primary endothelial cilia regulates blood pressure and cognition by Hannah C. Saternos, Kathleen V. Forero, Mahmood A. Meqdad, Raghad Buqaileh, Clare L. Sunderman, Gillian Gallagher, William S. Messer, Ashraf M. Mohieldin, Claudio A. Mucci, Sanjana Kumariya, Islam A. Osman, James P. Burkett, Wissam A. AbouAlaiwi

“…To further examine the role of vascular CHRM3 in vivo, we showed that endothelial CHRM3 knockout was associated with increased BP and attenuated acetylcholine-mediated vascular relaxation. …”
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Anti-Inflammatory Effect Fraction of Bletilla striata and Its Protective Effect on LPS-Induced Acute Lung Injury by Chunchun Zhang, Dandan Ning, Jieli Pan, Cheng Chen, Chengxian Gao, Zhishan Ding, Fusheng Jiang, Meiya Li

“…Additionally, component-knockout assay confirmed that these four quantitative components contributed significantly to the anti-inflammatory effect of EFBS. …”
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Inactivation of the TIM complex components leads to a decrease in the level of DNA import into Arabidopsis mitochondria by T. A. Tarasenko, K. D. Elizova, V. I. Tarasenko, M. V. Koulintchenko, Yu. M. Konstantinov

“…In this work, we studied the role of individual components of the TIM inner membrane translocase in the process of DNA import into isolated Arabidopsis mitochondria and their possible association with the porin VDAC1. Using knockout mutants for the genes encoding Tim17 or Tim23 protein isoforms, we demonstrated for the first time the involvement of these proteins in the import of DNA fragments of different lengths. …”
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STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation by Yu Qian Wong, Hongyan Xu, Qiang Wu, Xinyu Liu, Chengchen Lufei, Xiu Qin Xu, Xin-Yuan Fu

“…However, there is still lack of direct evidence on STAT3 functions in ESCs and embryogenesis because constitutive STAT3 knockout (KO) mouse is embryonic lethal at E6.5–E7.5, prior to potential functional role in early development can be assessed. …”
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Formation of the Ventricles by Catherine A. Risebro, Paul R. Riley

“…Several genes have been identified through studies on mouse knockout and transgenic models, which have contributed to our understanding of the molecular events governing these developmental processes. …”
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Cytokines in inflammatory bowel disease by P. L. Beck, J. L. Wallace

“…Over the past decade, much has been learned regarding the role of various cytokines in the pathogenesis of inflammatory bowel disease. Several cytokine ‘knockout’ models in mice have been shown to develop colitis, while alterations in the production of various cytokines has been documented in human Crohn's disease and ulcerative colitis. …”
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Downregulation of Long Noncoding RNA LINC00261 Attenuates Myocardial Infarction through the miR-522-3p/Trinucleotide Repeat-Containing Gene 6a (TNRC6A) Axis by Chaoxin Jiang, Qing Zhao, Chenlong Wang, Minyan Peng, Guoqing Hao, Zhifeng Liu, Wenjin Fu, Kewei Zhao

“…LINC00261 and TNRC6A were upregulated, while miR-522-3p was downregulated in coronary heart disease tissues with MI. Knockout of LINC00261 can increase the viability of cardiomyocytes and inhibit cell apoptosis. …”
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H. Pylori‐Facilitated TERT/Wnt/β‐Catenin Triggers Spasmolytic Polypeptide‐Expressing Metaplasia and Oxyntic Atrophy by Lijiao He, Xiao Zhang, Shengwei Zhang, Yi Wang, Weichao Hu, Jie Li, Yunyi Liu, Yu Liao, Xue Peng, Jianjun Li, Haiyan Zhao, Liting Wang, Yang‐Fan Lv, Chang‐Jiang Hu, Shi‐Ming Yang

“…Here, an increase in telomerase reverse transcriptase (TERT) protein expression is observed in chief cells upon infection with cagA‐positive H. pylori. Tert knockout significantly ameliorated H. pylori‐induced SPEM and single‐cell RNA sequencing demonstrated that the Wnt/β‐Catenin pathway is suppressed in gastric cells with Tert knockout. …”
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Adipose tissue-derived microRNA-450a-5p induces type 2 diabetes mellitus by downregulating DUSP10 by Jiaojiao Zhu, Yanting Hou, Wei Yu, Jingzhou Wang, Xiaolong Chu, Xueting Zhang, Huai Pang, Dingling Ma, Yihan Tang, Menghuan Li, Chenggang Yuan, Jianxin Xie, Cuizhe Wang, Jun Zhang

“…Moreover, the adipose Dicer-knockout mouse model was constructed to identify adipose tissue as the main source of microRNA-450a-5p. microRNA-450a-5p could inactivate the insulin signal pathway by targeting the inhibited Dual Specificity Phosphatase 10 (DUSP10) and inducing IR and glucose metabolism disorders in vitro cultured hepatocytes and adipocytes. …”
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Zinc finger proteins facilitate adaptation of a global insect pest to climate change by Tianpu Li, Jiao Guo, Guilei Hu, Fang Cao, Haiyin Su, Mengdi Shen, Huimin Wang, Minsheng You, Yuanyuan Liu, Geoff M. Gurr, Shijun You

“…We also employed CRISPR/Cas9 technology to create five stable homozygous knockout strains to elucidate ZFP functions in high-temperature tolerance. …”
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Cone photoreceptor phosphodiesterase PDE6H inhibition regulates cancer cell growth and metabolism, replicating the dark retina response by Ceren Yalaz, Esther Bridges, Nasullah K. Alham, Christos E. Zois, Jianzhou Chen, Karim Bensaad, Ana Miar, Elisabete Pires, Ruth J. Muschel, James S. O. McCullagh, Adrian L. Harris

“…PDE6H knockdown induced G1 cell cycle arrest and cell death and reduced mTORC1 signalling in cancer cell lines. Both knockdown and knockout of PDE6H resulted in the suppression of mitochondrial function. …”
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Patient-derived response estimates from zero-passage organoids of luminal breast cancer by Róża K. Przanowska, Najwa Labban, Piotr Przanowski, Russell B. Hawes, Kristen A. Atkins, Shayna L. Showalter, Kevin A. Janes

“…Combining sulforaphane with knockout of NQO1 (a phase II antioxidant response gene and downstream effector of sulforaphane) also yielded a breadth of organoid growth phenotypes, including growth inhibition with sulforaphane, growth promotion with NQO1 knockout, and growth antagonism when combined. …”
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HLA DR Genome Editing with TALENs in Human iPSCs Produced Immune-Tolerant Dendritic Cells by Yoo-Wook Kwon, Hyo-Suk Ahn, Jin-Woo Lee, Han-Mo Yang, Hyun-Jai Cho, Seok Joong Kim, Shin-Hyae Lee, Heung-Mo Yang, Hyun-Duk Jang, Sung Joo Kim, Hyo-Soo Kim

“…Teratomas formed by HLA DR knockout iPSCs did not express HLA DR, and dendritic cells differentiated from HLA DR knockout iPSCs reduced CD4+ T cell activation. …”
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MKRN1 promotes colorectal cancer metastasis by activating the TGF-β signalling pathway through SNIP1 protein degradation by Yi Zhang, Qin-shan Li, Hong-lin Liu, Hong-ting Tang, Han-lin Yang, Dao-qiu Wu, Yu-ying Huang, Li-cheng Li, Li-hong Liu, Meng-xing Li

“…Finally, using conditional knockout mice, intestinal lesions and metastatic liver microlesions were greatly reduced in the intestinal knockout MKRN1 group compared to that in the control group. …”
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