Skeletal Muscle-Specific CPT1 Deficiency Elevates Lipotoxic Intermediates but Preserves Insulin Sensitivity by Wanchun Shi, Siping Hu, Wenhua Wang, Xiaohui Zhou, Wei Qiu

“…Objective. By specific knockout of carnitine palmitoyl transferase 1b (CPT1b) in skeletal muscles, we explored the effect of CPT1b deficiency on lipids and insulin sensitivity. …”
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CD276 regulates the immune escape of esophageal squamous cell carcinoma through CXCL1–CXCR2 induced NETs by Yu Liang, Shuang Chen, Zhi Chen, Fang Peng, Qianwen Liu, Gan Xiong, Caihua Zhang, Maosheng Cheng, Rongsong Ling, Demeng Chen, Qimin Zhou

“…In vivo, we established a 4-nitroquinoline 1-oxide (4NQO)-induced CD276 knockout (CD276wKO) and K14cre; CD276 conditional knockout (CD276cKO) mouse model of ESCC to study the functional role of CD276 in ESCC. …”
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Astrocytic NLRP3 cKO mitigates depression-like behaviors induced by mild TBI in mice. by Hui-Tao Miao, Jun Wang, Jing-Jing Shao, Rong-Xin Song, Wen-Guang Li, Jian-Kai Sun, Shi-Yan Jia, Dong-Xue Zhang, Xiao-Ming Li, Jian-Yong Zhao, Li-Min Zhang

“…Notably, under mild TBI conditions, astrocyte-specific NLRP3 exhibited greater neuroprotective effects in female knockout mice compared to males. Conclusion: Astrocyte NLRP3 knockout demonstrated a protective mechanism in mice subjected to mild TBI, possibly attributed to the inhibition of pyroptosis through the NLRP3 signaling pathway in astrocytes.…”
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NLRP3 inflammasome-modulated angiogenic function of EPC via PI3K/ Akt/mTOR pathway in diabetic myocardial infarction by Jia-Peng Li, Shu Qiu, Guang-Jie Tai, Yi-Ming Liu, Wei Wei, Meng-Meng Fu, Pan-Qi Fang, Joseph Nicolao Otieno, Tungalag Battulga, Xiao-Xue Li, Ming Xu

“…The therapeutic effects of transplanted wild-type EPC, Nlrp3 knockout EPC, and Nlrp3 overexpression EPC were evaluated. …”
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Thioredoxin-interacting protein (TXNIP) inhibition promotes retinal ganglion cell survival and facilitates M1-like microglial transformation via the PI3K/Akt pathway in glaucoma by Junjue Chen, Huimin Zhong, Bingqiao Shen, Huan Yu, Yang Zhang, Ruiqi Han, Ping Huang, Shouyue Huang, Yisheng Zhong

“…Western blot, RT-PCR, immunofluorescence and F-VEP were used to detect neuroinflammation level, glial activation and RGCs survival in retina of wild type, TXNIP knockout and MCC950 treatment COH mice. Microglia high-pressure cultured model was constructed. …”
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Gαi1/3 signaling mediates IL-5-induced eosinophil activation and type 2 inflammation in eosinophilic chronic rhinosinusitis by Huanxia Xie, Jiang Ji, Zhichen Liu, Ning Lu, Yuqian Wei, Aina Zhou, Jisheng Liu, Qingqing Jiao, Qingqing Jiao

“…Signaling pathway activation in IL-5-induced Gαi1/3-knockout or knockdown mouse embryonic fibroblasts (MEFs) and eosinophils (EoL-1 cells) was detected by western blotting. …”
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Induction of phospholipase A2 group 4C by HCV infection regulates lipid droplet formation by Masahiko Ito, Jie Liu, Masayoshi Fukasawa, Koji Tsutsumi, Yumi Kanegae, Mitsutoshi Setou, Michinori Kohara, Tetsuro Suzuki

“…The amount and size of lipid droplets were reduced in PLA2G4C-knockout cells. Inhibition of NF-κB or c-Myc activity suppressed lipid droplet formation in HCV-infected cells. …”
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Deletion of bZIP Transcription Factor <i>PratfA</i> Reveals Specialized Metabolites Potentially Regulating Stress Response in <i>Penicillium raistrickii</i> by Anxin Zhang, Shu Zhang, Xinran Xu, Wen-Bing Yin

“…In this study, we purposefully altered <i>PratfA</i>, a crucial regulator associated with oxidative stress in <i>Penicillium raistrickii</i> CGMCC 3.1066. After the knockout of <i>PratfA</i>, a novel polyketide (PK) raistrilide A (<b>1</b>) and the known nonribosomal peptide (NRP) tunicoidine (<b>2</b>) subsequently disappeared. …”
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P-cadherin-dependent adhesions are required for single lumen formation and HGF-mediated cell protrusions during epithelial morphogenesis by Sydnie K. Tran, Jessanne Y. Lichtenberg, Corinne E. Leonard, Jessica R. Williamson, Hazel R. Sterling, Grace K. Panek, Amanda H. Pearson, Santiago Lopez, Christopher A. Lemmon, Daniel E. Conway, Priscilla Y. Hwang

“…CDH3 knockout cells exert lower forces on the surrounding environment compared to wild-type cells, suggesting CDH3 acts as a mechanosensor for stable cell protrusion establishment. …”
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Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice by Hua Yang, Mengjie Zhang, Jiahao Shi, Yunhe Zhou, Zhipeng Wan, Yicheng Wang, Yinghan Wan, Jun Li, Zhugang Wang, Jian Fei

“…We showed that SNAP-25 conditional knockout (cKO) mice exhibited typical schizophrenia-like phenotype. …”
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Circular RNA circFGFR1 Functions as an Oncogene in Glioblastoma Cells through Sponging to hsa-miR-224-5p by Qian Zhang, Shan Chen, Yingwei Zhen, Peng Gao, Zhenyu Zhang, Hao Guo, Yong Wang

“…The effect of circFGFR1 on glioma malignancy is abolished in CXCR4 knockout cells. Then, RIP, RNA pull-down, and luciferase reporter assay results showed that hsa-miR-224-5p directly binds to circFGFR1 and CXCR4 mRNA. …”
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HuR Plays a Positive Role to Strengthen the Signaling Pathways of CD4+ T Cell Activation and Th17 Cell Differentiation by Shiguang Yu, Morgan Tripod, Ulus Atasoy, Jing Chen

“…Here, we used HuR conditional knockout (HuR KO) CD4+ T cells to study mechanisms underlying HuR regulation of T cell activation and differentiation through distinct signaling pathways. …”
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ClaPEPCK4: target gene for breeding innovative watermelon germplasm with low malic acid and high sweetness by Congji Yang, Jiale Shi, Yuanyuan Qin, ShengQi Hua, Jiancheng Bao, Xueyan Liu, Yuqi Peng, Yige Gu, Wei Dong

“…It is interesting that the ClaPEPCK4 gene triggered the closure of leaf stomata under dark conditions in the knockout transgenic plants, which indicated its involvement in stomatal movement. …”
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Temporally and Spatially Controlled Age-Related Prostate Cancer Model in Mice by Sen Liu, Keyi Shen, Zixuan Li, Seleste Rivero, Qiuyang Zhang

“…Hereby, we present the prostate-specific temporally and spatially controlled Pten knockout PCa mouse model that can be induced using an adenovirus-based Cre-LoxP system. …”
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Calcium/calmodulin-dependent protein kinase II α and β differentially regulate mammalian sleep by Weiwen Yang, Jingyi Shi, Chenggang Li, Jingqun Yang, Jianjun Yu, Juan Huang, Yi Rao

“…While the basal sleep is reduced in Camk2a knockout mice, it remains unaltered in Camk2b mutants. …”
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The haploid induction ability analysis of various mutation of OsMATL and OsDMPs in rice by Siyi Liang, Qin Wen, Wenyu Lu, Guili Yang, Yingyun Yao, Hairong Cai, Jiafeng Wang, Ming Huang, Hui Wang, Tao Guo

“…Notably, in this study, the HI capacity of OsMATL knockout mutants in indica rice surpassed that of japonica rice knockout mutants, with the proton active site in the third exon exhibiting a higher HIR compared to the first and fourth exons. …”
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Extracellular matrix stiffness regulates colorectal cancer progression via HSF4 by Kangtao Wang, Siyi Ning, Shuai Zhang, Mingming Jiang, Yan Huang, Haiping Pei, Ming Li, Fengbo Tan

“…HSF4 expression increased with higher TNM stages, and its knockout significantly inhibited cell proliferation, migration, and invasion, especially on high-stiffness matrices. …”
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Crop genes modified using CRISPR/Cas system by A. M. Korotkova, S. V. Gerasimova, V. K. Shumny, E. K. Khlestkina

“…In most of these cases, modifications resulted in knockout of the genes such as negative growth and development regulators or negative regulators of plant resistance. …”
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CRISPR/cas9 Allows for the Quick Improvement of Tomato Firmness Breeding by Qihong Yang, Liangyu Cai, Mila Wang, Guiyun Gan, Weiliu Li, Wenjia Li, Yaqin Jiang, Qi Yuan, Chunchun Qin, Chuying Yu, Yikui Wang

“…We generated single and double gene knockout mutants utilizing the tomato genetic transformation system. …”
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HSP60 inhibits DF-1 apoptosis through its mitochondrial signal peptide by Shengliang Cao, Yanlan Li, Lele Chen, Xiaojing Lei, Xiujuan Feng, Yubao Li

“…This suggests that the HSP60 knockout induces apoptosis by up-regulating Caspase 3 and down-regulating Bax expression. …”
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