Metallothionein-3-mediated intracellular zinc mediates antioxidant and anti-inflammatory responses in the complete Freund’s adjuvant-induced inflammatory pain mouse model by Ngoc Buu Tran, Sook-Jeong Lee

“…We investigated the role of metallothionein-3 (MT3) in modulating complete Freund’s adjuvant (CFA)-induced intracellular Zn2+ activity in an MT3 knockout mouse model of inflammatory pain in the hind paw. …”
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Utilisation of an in vivo malaria model to provide functional proof for RhopH1/CLAG essentiality and conserved orthology with P. falciparum by Mitchell L. Trickey, Mrittika Chowdury, Georgina Bramwell, Natalie A. Counihan, Tania F. de Koning-Ward

“…Yet curiously, the combined knockout of both clag3 genes does not completely eliminate NPP function. …”
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Inflammatory adhesion mediates myocardial segmental necroptosis induced by mixed lineage kinase domain-like protein in acute myocardial infarction by Lijiang Wei, Naifu Wan, Wentong Zhu, Chenchen Liu, Zeyu Chen, Wuwei Rong, Lujun Zhang, Meifeng Xie, Yueqi Qin, Ting Sun, Qing Jing, Ankang Lyu

“…Although existing reports indicating that mixed lineage kinase domain-like protein (MLKL) is involved in macrophage necroptosis and inflammasome activation, the downstream mechanism of MLKL in necroptosis remain poorly characterized in AMI. Methods MLKL knockout mice (MLKLKO), RIPK3 knockout mice (RIPK3KO), and macrophage-specific MLKL conditional knockout mice (MLKLM−KO) were established. …”
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Lack of Hypoxia Inducible Factor-1α Influences on Macrophages Ability to Deal with Leishmania braziliensis In Vitro and Affects Pathology In Vivo by Rodrigo C.O. Sanches, Leonardo G. Vaz, Fabio V. Marinho, Erika S. Guimarães, Edgar M. Carvalho, Lucas P. Carvalho, Sergio C. Oliveira

“…Moreover, draining lymph nodes from myeloid HIF-1α–knockout mice also harbored increased populations of T cells. …”
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Keratinocyte Integrin α3β1 Promotes Efficient Healing of Wound Epidermis by Sanjana Dhulipalla, Giesse Albeche Duarte, Lei Wu, Mathieu R. DiPersio, John M. Lamar, C. Michael DiPersio, Whitney M. Longmate

“…This allows for the elucidation of α3β1-dependent wound healing in adult skin, free from compensatory mechanisms that may occur after embryonic deletion of epidermal α3β1 in the widely used constitutive α3β1-knockout mouse. We found that re-epithelializing wound gaps are larger in inducible α3 epidermal knockout mice than in control mice, indicating delayed healing, and that epidermal integrin α3β1 promotes healing of wounds, at least in part by enhancing keratinocyte proliferation. …”
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3-D Image Analysis of Fluorescent Drug Binding by M. Raquel Miquel, Venessa Segura, Z. Ali, M. P. D'Ocon, J. C. McGrath, Craig J. Daly

“…The histogram obtained for the 1D knockout aorta lay to the left of that of control and 1B knockout aorta, indicating a reduction in 1D receptors. …”
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Rodent Models for Investigating the Dysregulation of Immune Responses in Type 1 Diabetes by Feng-Cheng Chou, Heng-Yi Chen, Shyi-Jou Chen, Mei-Cho Fang, Huey-Kang Sytwu

“…Because the genetic manipulation of mice is well developed (transgenic, knockout, and conditional knockout/transgenic), most studies are performed using the nonobese diabetic (NOD) mouse model. …”
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Genetically edited human placental organoids cast new light on the role of ACE2 by Anya L. Arthurs, Bianca Dietrich, Martin Knöfler, Caleb J. Lushington, Paul Q. Thomas, Fatwa Adikusuma, Jessica M. Williamson, Susan Babikha, Tyla Damhuis, Tanja Jankovic-Karasoulos, Melanie D. Smith, Kirsty G. Pringle, Claire T. Roberts

“…Here, we report that ACE2 knockout results in delayed placental cell growth and increased cell death. …”
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CD2AP deficiency aggravates Alzheimer’s disease phenotypes and pathology through p38 MAPK activation by Yan-Yan Xue, Zhe-Sheng Zhang, Rong-Rong Lin, Hui-Fen Huang, Ke-Qing Zhu, Dian-Fu Chen, Zhi-Ying Wu, Qing-Qing Tao

“…Furthermore, the phenotypes of neuronal Cd2ap knockout were ameliorated by a p38 MAPK inhibitor. …”
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Exploring nagZ as a virulence biomarker and treatment target in Enterobacter cloacae by Xianggui Yang, Xuejing Yu, Li Zhou, Dan Wang, Weixian Chen

“…Deleting nagZ significantly reduced E. cloacae virulence in various infection models, including Galleria mellonella larvae, mice, and RAW264.7 cells. Moreover, nagZ knockout decreased the bacterium's ability to induce inflammatory factor levels, while complementing nagZ in knockout strains partially rescued this ability. …”
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mTOR Modulates the Endoplasmic Reticulum Stress-Induced CD4+ T Cell Apoptosis Mediated by ROS in Septic Immunosuppression by Hao Wang, Jianwei Chen, Guangxu Bai, Wen Han, Ran Guo, Na Cui

“…Additionally, we constructed T cell-specific mTOR and TSC1 gene knockout mice to determine the role of mTOR in ROS-mediated, ERS-induced CD4+ T cell apoptosis. …”
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Automatic Quantification of Atherosclerosis in Contrast-Enhanced MicroCT Scans of Mouse Aortas Ex Vivo by Vincent A. Stadelmann, Gabrielle Boyd, Martin Guillot, Jean-Guy Bienvenu, Charles Glaus, Aurore Varela

“…Approach and Results. 42 WT and 42 apolipoprotein E knockout mouse aortas were scanned. The walls, lumen, and plaque objects were segmented using dual-threshold algorithms. …”
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Claudin-11 regulates immunological barrier formation and spermatogonial proliferation through stem cell factor by Taichi Sugawara, Kayoko Sonoda, Nattapran Chompusri, Kazuhiro Noguchi, Seiji Okada, Mikio Furuse, Tomohiko Wakayama

“…Defective spermatogenesis in Cldn11-deficient mice was not restored on a recombination activating gene 2 (Rag2) knockout background lacking mature T and B lymphocytes. …”
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Diabetes Is Associated with Increased Autoreactivity of Mannan-Binding Lectin by Esben Axelgaard, Jakob Appel Østergaard, Steffen Thiel, Troels Krarup Hansen

“…After 24 weeks, fluorescently labelled recombinant MBL was injected intravenously in diabetic MBL knockout mice after which the distribution was investigated using in vivo fluorescence imaging. …”
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Identification of Binding Partners of Deafness-Related Protein PDZD7 by Haibo Du, Rui Ren, Panpan Chen, Zhigang Xu, Yanfei Wang

“…Unexpectedly, Cadm1 knockout mice show normal hearing threshold, which might be explained by the possible compensation by its homologs that are also expressed in the inner ear. …”
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Ly6E on tumor cells impairs anti-tumor T-cell responses: a novel mechanism of tumor-induced immune exclusion by Lan Hailin, Chen Yiting, Wu Yue, Li Lijun, Zhang Renlu, Chen Yunhan, Zhu Yanyang, Zhang Qiuyu

“…The enhanced anti-tumor effect of Ly6E knockout in tumor cells was dependent on T cell response and formed long-lasting memory. …”
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MUC2 expression modulates immune infiltration in colorectal cancer by Christophe M. Raynaud, Ayesha Jabeen, Eiman I. Ahmed, Eiman I. Ahmed, Satanay Hubrack, Apryl Sanchez, Shimaa Sherif, Ahmad A. Al-Shaibi, Bernice Lo, Bernice Lo, Jessica Roelands, Davide Bedognetti, Davide Bedognetti, Wouter Hendrickx, Wouter Hendrickx

“…In HT-29 cells, MUC2 knockout increased immune infiltration, while in LS-174T cells, compensatory expression of other mucins (MUC6, MUC5B) maintained the barrier. …”
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Divergent roles of m6A in orchestrating brown and white adipocyte transcriptomes and systemic metabolism by Ling Xiao, Dario F. De Jesus, Cheng-Wei Ju, Jiang-Bo Wei, Jiang Hu, Ava DiStefano-Forti, Valeria Salerno Gonzales, Tadataka Tsuji, Siying Wei, Matthias Blüher, Yu-Hua Tseng, Chuan He, Rohit N. Kulkarni

“…Stable METTL14-knockout hBAs/hWAs show METTL14-mediated m6A promotes mRNA decay of PTGES2 and CBR1 in hBAs and TRAIL and TNFR1 in hWAs. …”
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Essential and recurrent roles for hairpin RNAs in silencing de novo sex chromosome conflict in Drosophila simulans. by Jeffrey Vedanayagam, Marion Herbette, Holly Mudgett, Ching-Jung Lin, Chun-Ming Lai, Caitlin McDonough-Goldstein, Stephen Dorus, Benjamin Loppin, Colin Meiklejohn, Raphaëlle Dubruille, Eric C Lai

“…In the w[XD1] genetic background, knockout of nmy derepresses Dox and MDox in testes and depletes male progeny, whereas knockout of tmy causes misexpression of PDox genes and renders males sterile. …”
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Rice glycosyltransferase OsDUGT1 is involved in heat stress tolerance by glycosylating flavonoids and regulating flavonoid metabolism by Guang-rui Dong, Shu-man Zhao, Yi Ding, Yu-qing Ma, Xing-mei Ma, Chong-lin Liu, Bing-kai Hou

“…Its function was studied by overexpression and knockout methods. The results showed that under heat stress, OsDUGT1 overexpression lines (OsDUGT1-OE) increased the survival rate of rice, while Osdugt1 knockout lines (Osdugt1-ko) decreased the survival rate compared to wild type (ZH11). …”
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