Targeted Inhibition of GATA-3 by Pyrrothiogatain: Implications for Adipocyte Biology and Inflammatory Response by Shamma Almuraikhy, Maha Alser, Khaled Naja, Aisha Al-Malki, Nayef A. Mazloum, Mohamed A. Elrayess

“…Pyrrothiogatain treatment reduced cell proliferation in both wild-type and GATA-3 knockout 3T3-L1 cells, but did not alter their adipogenic capacity. …”
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Lack of adipocyte FAM20C improves whole body glucose homeostasis by Liping Deng, Yanshan Huang, Feifei Zhao, Puxin Chen, Xiaohong Huang

“…Here we generated adipocyte‐specific FAM20C knockout mice to investigate the role of FAM20C in adipose tissue expansion and obesity. …”
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Transcription factor-dependent regulatory networks of sexual reproduction in Fusarium graminearum by Wonyong Kim, Da-Woon Kim, Zheng Wang, Meng Liu, Jeffrey P. Townsend, Frances Trail

“…We evaluated in silico knockout impacts to the networks of the developmental phenotypes among the TFs and guided knockout transcriptomics experiments to properly assess regulatory roles of genes with same developmental phenotypes. …”
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Circular RNA circ-Olfm1 induces progression of Alzheimer’s disease by regulating FOXO3a by YANG Hongyan, LIAO Qirong, HOU Mingliang

“…ELISA revealed that knockout of FOXO3a significantly increased the levels of inflammatory factors IL-6, IL-1β, and TNF-α (P<0.01). …”
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Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells by Zixi Yin, Lingyi Chen

“…The CRISPR/Cas9 system provides a powerful method for the genetic manipulation of the mammalian genome, allowing knockout of individual genes as well as the generation of genome-wide knockout cell libraries for genetic screening. …”
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Functional Expression Study of Igf2 Antisense Transcript in Mouse by Carolina Duart-Garcia, Martin H. Braunschweig

“…Insulin-like growth factor antisense gene (Igf2as) expression was investigated in different mouse tissues during development, in differentiating C2C12 cells and in a ΔDMR1-U2 knockout mouse model. The expression levels of Igf2as were high in fetal and newborn liver and muscle tissues compared to adults. …”
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RBM4-mediated intron excision of Hsf1 induces BDNF for cerebellar foliation by Chiu-Lun Shen, Yu-Young Tsai, Shen-Ju Chou, Yao-Ming Chang, Woan-Yuh Tarn

“…Here, we show that Rbm4 knockout induced intron 6 retention of Hsf1, leading to downregulation of HSF1 protein and its downstream target BDNF. …”
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Stage-specific expression and divergent functions of two insulinase-like proteases associated with host infectivity in Cryptosporidium. by Yue Huang, Shifeng Pei, Xin Lv, Fuxian Yang, Xiaoqing Gong, Na Li, Yaqiong Guo, Yaoyu Feng, Lihua Xiao

“…Immunofluorescence analysis revealed that INS-19 and INS-20 are expressed at different developmental stages of the pathogen. Although knockout of either had no detectable effect on the in vitro growth of C. parvum, knockout of INS-20, deletion of its multiple domains, or mutation of the active motif in the functional domain reduced the intensity of C. parvum infection in IFN-γ knockout mice. …”
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Cromolyn sodium reduces LPS-induced pulmonary fibrosis by inhibiting the EMT process enhanced by MC-derived IL-13 by Cheng Tan, Hang Zhou, Qiangfei Xiong, Xian Xian, Qiyuan Liu, Zexin Zhang, Jingjing Xu, Hao Yao

“…Cromolyn sodium intervention and mast cell knockout alleviate the symptoms of pulmonary fibrosis probably via the PI3K/AKT/mTOR signaling pathway. …”
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γ-Aminobutyric Acid (GABA) Metabolic Bypass Plays a Crucial Role in Stress Tolerance and Biofilm Formation in <i>C. sakazakii</i> ATCC 29544 by Jiangchao Wu, Yigang Yu, Fengsong Liu, Yifang Cao, Jiahao Ren, Yiting Fan, Xinglong Xiao

“…The GABA content and survival rates of both the wild-type and knockout strains were compared under various stress conditions. …”
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HACE1 protects against myocardial ischemia–reperfusion injury via inhibition of mitochondrial fission in mice by Bang-Xia Liu, Juan Zheng, Zhan-Wei Tang, Lei Gao, Meng Wang, Ying Sun, Chen Chen, Heng-Chen Yao

“…Methods Male C57BL6 mice with HACE1 knockout (KO) were subjected to 30 min of ischemia via ligation of the left anterior descending artery, followed by 0, 2, 6, or 24 h of reperfusion. …”
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Neuronal BDNF Signaling Is Necessary for the Effects of Treadmill Exercise on Synaptic Stripping of Axotomized Motoneurons by Joey Krakowiak, Caiyue Liu, Chandana Papudesu, P. Jillian Ward, Jennifer C. Wilhelm, Arthur W. English

“…No further reduction in coverage was found if nerves were cut in knockout mice. Two weeks of moderate daily treadmill exercise following nerve injury in these BDNF knockout mice did not affect synaptic inputs onto motoneurons. …”
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Manipulations with early mouse embryos for generation of genetically modified animals by A. N. Korablev, I. A. Serova, B. V. Skryabin

“…Until last time, the CRISPR/Cas9 system noticeably simplified the preparation of knockout or transgenic mice. CRISPR/Cas9 technology was successfully applied for gene knockout and knock-in, generation of large deletions or directed insertions in targeted genome regions in embryonic stem cells (ESCs).When injected into blastocysts, such  modified ESCs are able to generate chimeras producing gametes with an identical genotype with ESC. …”
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Ficolin B in Diabetic Kidney Disease in a Mouse Model of Type 1 Diabetes by Charlotte Berg Holt, Jakob Appel Østergaard, Esben Axelgaard, Gitte Krogh Nielsen, Yuichi Endo, Steffen Thiel, Troels Krarup Hansen

“…We induced diabetes with streptozotocin in female wild-type mice and ficolin B knockout mice and included two similar nondiabetic groups. …”
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CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris by Kaidi Chen, Gulikezi Maimaitirexiati, Qiannan Zhang, Yi Li, Xiangjian Liu, Hongting Tang, Xiang Gao, Bo Wang, Tao Yu, Shuyuan Guo

“…The efficiency of dual-site knockout ranged from 60 % to 100 %, with functional genes knockout achieving approximately 60 % (Δaox1Δgut1), while dual neutral sites knockout reached 100 %. …”
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Major facility superfamily sugar transporter protein SsMFSST1 regulates Sporisorium scitamineum mating, pathogenicity, and sugar transport/absorption by Yi Zhang, Yongding He, Meixin Yan, Shilong Zhang, Sisi Zhou, Wankuan Shen

“…The research results showed that the sexual mating ability of the knockout mutants significantly decreased compared with the wild type, while the sexual mating ability of the complementary mutants was almost restored to the level of the wild type. …”
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Epac1 Restores Normal Insulin Signaling through a Reduction in Inflammatory Cytokines by Elizabeth Curtiss, Youde Jiang, Li Liu, Claire Hawthorne, Jessica Zhang, Jena J. Steinle

“…Confocal microscopy was performed to localize the insulin receptor in the retina of Epac1 floxed and endothelial cell-specific Epac1 knockout mice. Whole retinal lysates from Epac1 floxed and endothelial cell-specific Epac1 knockout mice were used to investigate proteins involved in the insulin signaling cascade. …”
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Role of intestinal mucin-2 in the effectiveness of the treatment of Helicobacter spp. infection in laboratory mice by E. A. Litvinova, M. D. Belyaev, A. V. Prokhortchouk, V. S. Korostina, E. B. Prokhortchouk, E. N. Kozhevnikova

“…Moreover, the mortality of Muc2 knockout mice during the antibacterial treatment was 60 %. …”
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Diacylglycerol kinase δ is required for skeletal muscle development and regeneration by Hiromichi Sakai, Chiaki Murakami, Mayumi Takechi, Takeshi Urano, Fumio Sakane

“…The knockout mice showed a significant body weight reduction and apparent mass decrease in skeletal muscle, including the tibialis anterior (TA) muscle. …”
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The critical role of MLKL in hemorrhagic stroke and the therapeutic potential of its associated protein network by Yi Wang, Yi Wang, Moran Xu, Xiaoli Zuo, Sheng Wang, Yong Yu, Zhaobing Gao, Zhaobing Gao, Jingbo Qie, Ye Jiang, Fang Huang, Bingqing Xia, Bingqing Xia

“…However, we found that Mlkl knockout alone was insufficient to fully reverse neuroinflammation and pathological damage. …”
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