Induced protein expression in Leptospira spp. and its application to CRISPR/Cas9 mutant generation by L. G. V. Fernandes, A. L. T. O. Nascimento, J. E. Nally

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Study about the Expression of CypA Proteins and Its Blocking Effect in the Cerebellum from FMR1 Gene Knockout Mice by Electroacupuncture of DU1 Acupoint by Dong LIN, Wanping BU, Xiaoting YANG

Subjects: “…gene knockout…”
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The protective effect of oleic acid on Lactiplantibacillus plantarum during freeze-drying by Yongjun Xia, Yizhen Wang, Jing Pu, Yan Wu, Zhiqiang Xiong, Xin Song, Hui Zhang, Lianzhong Ai, Guangqiang Wang

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OsHMA3 overexpression works more efficiently in generating low-Cd rice grain than OsNramp5 knockout mutation by Yuejing Gui, Joanne Teo, Dongsheng Tian, Raji Mohan, Zhongchao Yin

“…In this study, we compared the efficiency of OsNramp5 knockout mutation and OsHMA3 overexpression in reducing Cd content in rice grain. …”
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Using intermediate energy knockout, pickup, and charge exchange reactions with invariant mass spectroscopy for investigating nuclear structure beyond the proton drip line by L. G. Sobotka, L. G. Sobotka, R. J. Charity

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Enhanced membrane protein production in HEK293T cells via ATF4 gene knockout: A CRISPR-Cas9 mediated approach by Byung-Jo Choi, Ba Reum Kim, Ho Joong Choi, Ok-Hee Kim, Say-June Kim

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The small GTPases FoRab5, FoRab7, and FoRab8 regulate vesicle transport to modulate vegetative development and pathogenicity in Fusarium oxysporum f. sp. conglutinans by Xiangyu Tan, Lin Chen, Ying Chen, Yuxin Li, Lihan Lu, Erfeng Li

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TAB2 deficiency induces dilated cardiomyopathy by promoting mitochondrial calcium overload in human iPSC-derived cardiomyocytes by Wenrui Sun, Jianchao Zhang, Shuang Li, Wanrong Fu, Yangyang Liu, Mengduan Liu, Jianzeng Dong, Xiaoyan Zhao, Xiaowei Li

Subjects: “…TAB2 knockout…”
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Conducting and automating the water Morris maze test in SPF conditions by N. V. Khotskin, V. A. Kulikov, E. L. Zavyalov, D. V. Fursenko, A. V. Kulikov

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Knockout of BnaX.SGT.a caused significant sinapine reduction in transgene-free rapeseed mutants generated by protoplast-based CRISPR RNP editing by Oliver Moss, Xueyuan Li, Eu Sheng Wang, Selvaraju Kanagarajan, Rui Guan, Emelie Ivarson, Li-Hua Zhu

“…We achieved successful knockout of the BnaX.SGT.a paralogues, with an average mutation efficiency of over 30%. …”
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Knockout of the inhibitory receptor TIGIT enhances the antitumor response of ex vivo expanded NK cells and prevents fratricide with therapeutic Fc-active TIGIT antibodies by Dean A Lee, Jeremiah L Oyer, Md Faqrul Hasan, Tayler J Croom-Perez, Liza D Robles-Carrillo, Sanjana Kumar, Brendan W Andersen, Brian P Tullius, Alicja J Copik, Amanda R Campbell, Thomas A Dieffenthaller, Catherine A Cash, Jonathan E Eloriaga, Meisam Naeimi Kararoudi

“…Since NK cells express TIGIT and Fc-receptor CD16, Fc-active anti-TIGIT may deplete NK cells via fratricide, which has not been studied.Methods CRISPR-Cas9-based TIGIT knockout (KO) was performed in expanded NK cells. Phenotypic and transcriptomic properties of TIGIT KO and wild-type (WT) NK cells were compared with flow cytometry, CyTOF, and RNA sequencing. …”
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Host Susceptibility to Brucella abortus Infection Is More Pronounced in IFN-γ knockout than IL-12/β2-Microglobulin Double-Deficient Mice by Ana Paula M. S. Brandão, Fernanda S. Oliveira, Natalia B. Carvalho, Leda Q. Vieira, Vasco Azevedo, Gilson C. Macedo, Sergio C. Oliveira

“…Herein, IFN-γ and IL-12/β2-microglobulin (β2-m) knockout mice were used to determine whether CD8+ T cells and IL-12-dependent IFN-γ deficiency would be more critical to control B. abortus infection compared to the lack of endogenous IFN-γ. …”
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Influence of urokinase gene-knockout in C57BL/6-PlautmI. IBugThisPlau6FDhu/GFDhu mice on growth factors in malignant melanoma by E. M. Frantsiyants, I. V. Kaplieva, I. V. Neskubina, V. A. Bandovkina, L. K. Trepitaki, E. I. Surikova, N. D. Cheryarina, L. A. Nemashkalova, N. S. Lesovaya

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Alterations of Amino Acids and Monoamine Metabolism in Male Fmr1 Knockout Mice: A Putative Animal Model of the Human Fragile X Mental Retardation Syndrome by Michael Gruss, Katharina Braun

“…We describe morphological and behavioral abnormalities for both affected humans and Fmr1 knockout mice, a putative animal model for the human Fragile X syndrome. …”
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Magnetic Resonance Imaging Visualization of Vulnerable Atherosclerotic Plaques at the Brachiocephalic Artery of Apolipoprotein E Knockout Mice by the Blood-Pool Contrast Agent B22956/1 by Cinzia Parolini, Marco Busnelli, Giulia S. Ganzetti, Federica Dellera, Stefano Manzini, Eugenio Scanziani, Jason L. Johnson, Cesare R. Sirtori, Giulia Chiesa

“…The aim of this study was to identify, by magnetic resonance imaging (MRI), the ability of the blood-pool contrast agent B22956/1 to detect atherosclerotic plaques developing at the brachiocephalic artery of apolipoprotein E knockout (apoE-KO) mice and to possibly identify vulnerable atherosclerotic lesions. …”
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Comparison of natural and artificial vasopressin deficiency: why the latter is lethal? by D. Zelena

Subjects: “…brattleboro rat, vasopressin knockout mice, vasopressin gene, vasopressin receptors, hypovolaemia.…”
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Thyroid hormone receptor- and stage-dependent transcriptome changes affect the initial period of Xenopus tropicalis tail regeneration by Shouhong Wang, Liezhen Fu, Bin Wang, Yanmei Cai, Jianping Jiang, Yun-Bo Shi

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CRISPR/Cas9-editing of PRNP in Alpine goats by Aurélie Allais-Bonnet, Christophe Richard, Marjolaine André, Valérie Gelin, Marie-Christine Deloche, Aurore Lamadon, Gwendoline Morin, Béatrice Mandon-Pépin, Eugénie Canon, Dominique Thépot, Johann Laubier, Katayoun Moazami-Goudarzi, Ludivine Laffont, Olivier Dubois, Thierry Fassier, Patrice Congar, Olivier Lasserre, Tiphaine Aguirre-Lavin, Jean-Luc Vilotte, Eric Pailhoux

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Advancing Obsessive–Compulsive Disorder Research: Insights from Transgenic Animal Models and Innovative Therapies by Xinyuejia Huang, Linglong Xiao, Mengqi Wang, Yang Wu, Hao Deng, Wei Wang

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Positron Emission Tomographic Imaging of the Cannabinoid Type 1 Receptor System with [C]OMAR ([C]JHU75528): Improvements in Image Quantification Using Wild-Type and Knockout Mice by Raúl Herance, Santiago Rojas, Sergio Abad, Xavier Jiménez, Juan Domingo Gispert, Olga Millán, Elena Martín-García, Aurelijus Burokas, Miquel Àngel Serra, Rafael Maldonado, Deborah Pareto

“…Wild-type (WT) andCB1 knockout (KO) animals were imaged at baseline and after pretreatment with blocking doses of rimonabant. …”
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