Intercellular TIMP-1-CD63 signaling directs the evolution of immune escape and metastasis in KRAS-mutated pancreatic cancer cells by Chu-An Wang, Ya-Chin Hou, Yi-Kai Hong, Yu-Jing Tai, Chieh Shen, Pei-Chi Hou, Jhao-Lin Fu, Cheng-Lin Wu, Siao Muk Cheng, Daw-Yang Hwang, Yung-Yeh Su, Yan-Shen Shan, Shaw-Jenq Tsai

“…Genetically engineered pancreas-specific Kras-mutated, dual specificity phosphatase-2 (Dusp2) knockout mouse models were established. Human and mouse primary pancreatic cancer cell lines were used for in vitro assessment of cancer characteristics. …”
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Leucine-rich alpha-2-glycoprotein 1 deficiency suppresses ischemia–reperfusion injury-induced renal fibrosis by Naohito Okami, Hiromichi Wakui, Kengo Azushima, Tomohito Miyazawa, Eisuke Kubo, Shunichiro Tsukamoto, Mari Sotozawa, Shinya Taguchi, Shingo Urate, Kohei Ishiga, Sho Kinguchi, Tomohiko Kanaoka, Kouichi Tamura

“…Unilateral IRI was induced in C57BL/6J wild-type (WT) mice and systemic LRG1 knockout (KO) mice. In C57BL/6J WT mice, renal LRG1 mRNA expression was significantly elevated on the ischemia/reperfusion side compared to the sham side over a 28-day period. …”
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The Rickettsia actin-based motility effectors RickA and Sca2 contribute differently to cell-to-cell spread and pathogenicity by Cuong J. Tran, Zahra Zubair-Nizami, Ingeborg M. Langohr, Matthew D. Welch

“…We further compared the roles of RickA and Sca2 in vivo following intradermal (i.d.) infection of Ifnar1−/−; Ifngr1−/− mice carrying knockout mutations in the genes encoding the receptors for IFN-I (Ifnar1) and IFN-γ (Ifngr1), which exhibit eschars and succumb to infection with wild-type (WT) R. parkeri. …”
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Pan-cancer and experimental analyses reveal the immunotherapeutic significance of CST2 and its association with stomach adenocarcinoma proliferation and metastasis by Dan Huang, Jing Li, Zhijun He, Wenjing Liang, Likun Zhong, Jun Huang, Yinteng Wu, Shijian Zhao

“…Finally, in gastric cancer cell lines, we will investigate the impact of CST2 knockout on expression levels, clonal proliferation, cell apoptosis, and cell migration.ResultsCST2 exhibits abnormal overexpression in multiple tumors. …”
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Deficiency of purinergic P2Y2 receptor impairs the recovery after renal ischemia-reperfusion injury and accelerates renal fibrosis and tubular senescence in mice by Kyuho Jeong, Jihyun Je, Theodomir Dusabimana, Jacques Karekezi, Tatang Aldi Nugroho, Edvard Ntambara Ndahigwa, Hyun Joon Kim, Seung Pil Yun, Hye Jung Kim, Hwajin Kim, Sang Won Park

“…To investigate the role of the purinergic P2Y2 receptor (P2Y2R) in these processes, we used P2Y2R knockout (KO) mice subjected to IR. KO mice showed severe kidney dysfunction and structural damage compared to WT mice. …”
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Exploring the functional role of POU5F1 using CRISPR RNP electroporation of zygote (CRISPR-EZ) in buffaloes by Meeti Punetha, Dharmendra Kumar, Suman Choudhary, Sheetal Saini, Kamlesh Kumari Bajwa, Surabhi Sharma, Manu Mangal, P. S. Yadav, T. K. Datta

“…We targeted the buffalo POU5F1 gene using the abovementioned parameters, which caused nonsense-mediated mRNA decay and led to complete knockout (KO) of the POU5F1 gene. We analyzed the mutation rates and mosaic mutations using tracking of Indels by decomposition (TIDE) software. …”
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Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue Lipolysis by Liming Liu, Cuiqing Zhao, Ying Yang, Xiaoxia Kong, Tuo Shao, Li Ren, Xinyu Zhuang, Baishuang Yin, Gerald Dryden, Craig McClain, Weimin Luan, Wenke Feng

“…The role of FGF21 was investigated using antibody neutralization or knockout (KO) mice. Lipolysis index and adipose lipolytic enzymes were determined. …”
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Improving the integrity and reproducibility of research that uses antibodies: a technical, data sharing, behavioral and policy challenge by M. Biddle, P. Stylianou, M. Rekas, A. Wright, J. Sousa, D. Ruddy, M. I. Stefana, K. Kmiecik, A. Bandrowski, R.A. Kahn, C. Laflamme, E. M. Krockow, H.S. Virk

“…The open-science company YCharOS works with major antibody manufacturers and knockout cell line producers to characterize antibodies, identifying high-performing renewable antibodies for many targets in neuroscience. …”
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Elite Alleles of EPE1 Identified via Genome-wide Association Studies Increase Panicle Elongation Length in Rice by Hao Sun, Qiqi Yao, Mei Hai, Tianhu Li, Jinghan Sun, Zhengbo Liu, Yang Ang, Yingying Zhao, Yanan Zhang, Xianping Cheng, Tao Huang, Yinping Chang, Mingyu Du, Erbao Liu

“…Compared with the short PELs of wild plants, mutant plants with Cas9-induced knockout (KO) of OsGA2ox9 exhibit long PELs. Therefore, OsGA2ox9 was identified as the key gene controlling PEL in rice. …”
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The Role of β-Catenin in Th1 Immune Response against Tuberculosis and Profiles of Expression in Patients with Pulmonary Tuberculosis by Kunlong Xiong, Jinxia Niu, Ruijuan Zheng, Zhonghua Liu, Yanzheng Song, Lin Wang, Changtai Zhu, Lin Fan

“…In this study, CD4+ T cell-specific β-catenin conditional knockout mice (β-CAT-cKO mice) were aerosol infected with Mycobacteria tuberculosis (Mtb) H37RV with wild-type mice as controls. …”
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RETRACTED ARTICLE: P16INK4a Deletion Ameliorated Renal Tubulointerstitial Injury in a Stress-induced Premature Senescence Model of Bmi-1 Deficiency by Jianliang Jin, Jianguo Tao, Xin Gu, Zhenzhen Yu, Rong Wang, Guoping Zuo, Qing Li, Xianhui Lv, Dengshun Miao

“…Abstract To determine whether p16INK4a deletion ameliorated renal tubulointerstitial injury by inhibiting a senescence-associated secretory phenotype (SASP) in Bmi-1-deficient (Bmi-1−/−) mice, renal phenotypes were compared among 5-week-old Bmi-1 and p16INK4a double-knockout, and Bmi-1−/− and wild-type mice. Fifth-passage renal interstitial fibroblasts (RIFs) from the three groups were analyzed for senescence and proliferation. …”
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Kaempferol protects against high-salt-induced hypertension and vascular endothelial injury by inhibiting ferroptosis through the ATF4/ACSL4 pathway by Qiuer Liang, Minghao Chen, Guangtian Chen, Pengli Xu, Lai Kwan Lam, Pengcheng Xie, Ting Xie, Wanqing Tu, Tianhao Liu, Xiaopeng Peng, Haoyao Yuan, Liguo Chen, Ya Xiao

“…Using an 8 % high-salt diet model, we evaluated the effects of Kae (200 mg/kg/day) in wild-type (WT) and activating transcription factor 4 (ATF4+/−) knockout mice. Additionally, human aortic endothelial cells (HAECs) cultured under high-salt conditions were used to assess Kae's protective effects, with ATF4 and acyl-CoA synthetase long-chain family member 4 (ACSL4) knocked down via siRNA transfection. …”
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Germinal Center B Cells are Uniquely Targeted by Antibody-Suppressor CXCR5+CD8+ T Cells by Jason M. Zimmerer, PhD, Sachi Chaudhari, BS, Kavya Koneru, BS, Jing L. Han, MD, PhD, Mahmoud Abdel-Rasoul, MA, Hope Uwase, MA, Tai Yi, MD, Christopher K. Breuer, MD, Ginny L. Bumgardner, MD, PhD

“…In vivo CD8-mediated cytotoxicity to wild-type germinal center (GC) B cells or wild-type CD4+ T follicular helper cells (TFH cells) was assessed in RAG1 knockout mice. The impact of in vivo adoptive transfer of CD8+ TAb-supp cells into hepatocyte or kidney transplant recipients on the quantity of lymphoid immune-cell subsets was assessed. …”
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Dual inhibition of BET and EP300 has antitumor activity in undifferentiated pleomorphic sarcomas and synergizes with ferroptosis induction by Stéphanie Verbeke, Aurélien Bourdon, Mathilde Lafon, Vanessa Chaire, Bertolo Frederic, Amina Naït Eldjoudi, Marie-Alix Derieppe, Francis Giles, Antoine Italiano

“…The most potent inhibitor was evaluated in vivo in two animal models and the mechanisms of action were investigated by RNA sequencing, Western blotting and immunofluorescence staining. A CRISPR knockout screen was performed to identify resistance mechanisms. …”
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Regulation of enzymatic lipid peroxidation in osteoblasts protects against postmenopausal osteoporosis by Qiong-Yi Zhang, Hai-Biao Gong, Man-Ya Jiang, Fujun Jin, Guan Wang, Chang-Yu Yan, Xiang Luo, Wan-Yang Sun, Shu-Hua Ouyang, Yan-Ping Wu, Wen-Jun Duan, Lei Liang, Yun-Feng Cao, Xin-Xin Sun, Meijing Liu, Gen-Long Jiao, Hua-Jun Wang, Kurihara Hiroshi, Xiaogang Wang, Rong-Rong He, Yi-Fang Li

“…The deficiency of estrogen reduces GPX4 expression and increases phospholipid peroxidation in osteoblasts. Inhibition or knockout of GPX4 impairs osteoblastogenesis, while the elimination of phospholipid peroxides rescues bone formation and mitigates osteoporosis. …”
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The Legionella pneumophila effector PieF modulates mRNA stability through association with eukaryotic CCR4−NOT by Harley O'Connor Mount, Malene L. Urbanus, Francesco Zangari, Anne-Claude Gingras, Alexander W. Ensminger

“…Heterologous expression of PieF phenocopies knockout of the CNOT7 ortholog (POP2) in Saccharomyces cerevisiae, resulting in 6-azauracil sensitivity. …”
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Blockades of effector T cell senescence and exhaustion synergistically enhance antitumor immunity and immunotherapy by Xia Liu, Emily Shaw, Fusheng Si, Guangyong Peng, Yan Tao, Feiya Ma, David Bagley, Yuanqin Zhang

“…Loss-of-function strategies with pharmacological inhibitors and the knockout mouse model were used to identify the potential molecules and pathways involved in T cell senescence. …”
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The cAMP Pathway Amplifies Early MyD88-Dependent and Type I Interferon-Independent LPS-Induced Interleukin-10 Expression in Mouse Macrophages by Orna Ernst, Yifat Glucksam-Galnoy, Muhammad Athamna, Iris Ben-Dror, Hadar Ben-Arosh, Galit Levy-Rimler, Iain D. C. Fraser, Tsaffrir Zor

“…IL-10 promoter reporter activity was synergistically induced by cAMP elevation in macrophages stimulated by an agonist of either TLR4, TLR2/6, or TLR7, receptors which signal via MyD88, but not by an agonist of TLR3 which signals independently of MyD88. Moreover, MyD88 knockout largely reduced the synergistic IL-10 expression, indicating that MyD88 is required for the synergism displayed by LPS with cAMP. …”
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Presynaptic CRF1 Receptors Mediate the Ethanol Enhancement of GABAergic Transmission in the Mouse Central Amygdala by Zhiguo Nie, Eric P. Zorrilla, Samuel G. Madamba, Kenner C. Rice, Marissa Roberto, George Robert Siggins

“…We recently reported that the ethanol augmentation of GABAergic synaptic transmission in rat CeA involves CRF1 receptors, because both CRF and ethanol significantly enhanced the amplitude of evoked GABAergic inhibitory postsynaptic currents (IPSCs) in CeA neurons from wild-type (WT) and CRF2 knockout (KO) mice, but not in neurons of CRF1 KO mice. …”
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Adrenomedullin 2 attenuates anxiety-like behaviors by increasing IGF-II in amygdala and re-establishing blood–brain barrier by Denian Wang, Zhi Yang, Pengfei Wu, Qingyan Li, Chunyan Yu, Ying Yang, Yuefan Du, Mengwei Jiang, Junpeng Ma

“…Furthermore, as evidenced by increased Evans blue staining and decreased VE-cadherin levels, the BBB exhibited dysfunction in ADM2 knockout mice with anxiety-like behaviors. In in vitro studies, ADM2 administration promoted the expression of VE-cadherin and decreased IGF-II leakage through the endothelial barrier in a BBB model. …”
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