Chicken C4BPM facilitates replication of H5N1 and H9N2 Avian Influenza Viruses by inhibiting the production of type I interferons by Huizi Li, Shishi Wang, Ruihong Guo, Xijiao Chen, Yuanyuan Huang, Weixin Jia, Zhangyong Ning

“…To verify the role of C4BPM in replication of AIVs, C4BPM knockout (C4BPM-KO) and stable overexpressing (C4BPM-overexpressing) DF-1 cells were generated using lentivirus-mediated CRISPR/Cas9 gene editing technology and molecular cloning strategies. …”
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A novel technique of cryodenervation for murine vagus nerve: implications for acute lung inflammation by Di Wu, Ximing Liao, Jing Gao, Kun Wang, Wujian Xu, Feilong Wang, Zhixian Jin, Dandan Wu, Qiang Li, Wei Gao

“…We then constructed the acute lung injury (ALI) model triggered by lipopolysaccharide (LPS) to verify the effect of VSCD in the resolution of pulmonary inflammation. Besides, the IL-10 knockout mice were also applied to explain the underlying mechanism of the protective activity of VSCD in ALI mice. …”
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Anti-IL-6 Receptor Antibody Causes Less Promotion of Tuberculosis Infection than Anti-TNF-𝛼 Antibody in Mice by Masaji Okada, Yoko Kita, Noriko Kanamaru, Satomi Hashimoto, Yasushi Uchiyama, Masahiko Mihara, Yoshikazu Inoue, Yoshiyuki Ohsugi, Tadamitsu Kishimoto, Mitsunori Sakatani

“…Similar results were obtained in IL-6 knockout (KO) mice, compared with TNF receptor 1 (TNFR1) KO and TNFR1/IL-6 double KO (DKO) mice. …”
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Caerin 1.1/1.9-mediated antitumor immunity depends on IFNAR-Stat1 signalling of tumour infiltrating macrophage by autocrine IFNα and is enhanced by CD47 blockade by Junjie Li, Yuandong Luo, Quanlan Fu, Shuxian Tang, Pingping Zhang, Ian H. Frazer, Xiaosong Liu, Tianfang Wang, Guoying Ni

“…In this study, we explored the impact of F1/F3 on the tumor microenvironment in a transplanted B16 melanoma model, and further investigated the mechanism of action of F1/F3 using monoclonal antibodies to deplete relevant cells, gene knockout mice and flow cytometry. We show that F1/F3 is able to inhibit the growth of melanoma B16 tumour cells both in vitro and in vivo. …”
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ERK1/2 Inhibition Alleviates Diabetic Cardiomyopathy by Suppressing Fatty Acid Metabolism by Erin McLean, Caroline De Roo, Annabel Maag, Megan Coble, Jefferson Cano, Ruijie Liu

“…In contrast, elevated phosphorylation of ERK1/2 in Dusp6/8 knockout (DKO) mice resulted in fibrosis. Mechanistically, ERK1/2 activation enhanced the expression of fatty acid metabolism genes PPARα, CPT1A, and FACS in the heart, which was reversed by U0126 treatment. …”
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TaWI12 may be involved in pistillody and leaf cracking in wheat by Yuhuan Guo, Yan Zhang, Yuhao Li, Yichao Wu, Mingli Liao, Zhengsong Peng, Zaijun Yang, Yonghong Zhou

“…We noticed two distinct phenotypic differences in the knockout mutant. First, we observed the different degrees of homologous conversion of stamens to pistils in the single mutant TaWI12-4D. …”
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Roles of the <i>Sec2p</i> Gene in the Growth and Pathogenicity Regulation of <i>Aspergillus fumigatus</i> by Yuhuan Liu, Shumi Shang, Cong Liu, Yichen Liu, Keyang Xu, Dan He, Li Wang

“…Transmission electron microscopy revealed that the vacuoles of the gene knockout strain <i>ΔSec2p</i> accumulated more autophagosomes, indicating inhibition of autophagosome degradation. …”
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miR-210 in Exosomes Derived from Macrophages under High Glucose Promotes Mouse Diabetic Obesity Pathogenesis by Suppressing NDUFA4 Expression by Feng Tian, Ping Tang, Zhilian Sun, Ruifen Zhang, Danhua Zhu, Junying He, Jixing Liao, Qinghua Wan, Jie Shen

“…Furthermore, mice with miR-210 knockout showed greatly repressed diabetic obesity development. …”
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Effect of custom-designed transfection buffer on delivery of genome modification components into primary cells of buffalo, cattle, goats, and sheep by Shreya Malhotra, Priyanka Singh, Aseem Tara, Bosco Jose, Devika Gautam, Ram Prasad, Gaurav Tripathi, Sacchinandan De, Naresh L. Selokar

“…In addition, we also generated MSTN (myostatin) KO (Knockout) cell clones from these cell populations, in which the efficiency of single-cell clone generation was high for goats and sheep cells. …”
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An Attenuated Cytomegalovirus Vaccine with a Deletion of a Viral Chemokine Gene Is Protective against Congenital CMV Transmission in a Guinea Pig Model by Michael P. Leviton, Juan C. Lacayo, K. Yeon Choi, Nelmary Hernandez-Alvarado, Andrew Wey, Mark R. Schleiss

“…To resolve this problem, this study employed guanosyl phosphoribosyl transferase (gpt) mutagenesis to generate a recombinant guinea pig CMV (GPCMV) with a knockout of a viral chemokine gene, GPCMV MIP (gp1). …”
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Developmental deficits, synapse and dendritic abnormalities in a Clcn4 KO autism mice model: endophenotypic target for ASD by Seong Mi Lee, Yura Choi, Dayeon Kim, Ha Jin Jeong, Young Ho Do, Sohee Jung, Bomee Lee, Hyung Jun Choi, Suhyeon Kim, Jung-Min Oh, Songhee Jeon, Jinju Han, Yeni Kim

“…Abstract Autism spectrum disorder (ASD) is linked to ion channel dysfunction, including chloride voltage-gated channel-4 (CLCN4). We generated Clcn4 knockout (KO) mice by deleting exon 5 of chromosome 7 in the C57BL/6 mice. …”
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Neuronal density in the brain cortex and hippocampus in Clsnt2-KO mouse strain modeling autistic spectrum disorder by I. N. Rozhkova, S. V. Okotrub, E. Yu. Brusentsev, E. E. Uldanova, E. А. Chuyko, T. V. Lipina, T. G. Amstislavskaya, S. Ya. Amstislavsky

“…This mouse strain was obtained by a knockout of the calsinthenin-2 gene (Clsnt2) in C57BL/6J mice; the latter (wild type) was used as controls. …”
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hnRNPU-mediated pathogenic alternative splicing drives gastric cancer progression by Guoguo Jin, Yanming Song, Shaobo Fang, Mingyang Yan, Zhaojie Yang, Yang Shao, Kexin Zhao, Meng Liu, Zhenwei Wang, Zhiping Guo, Zigang Dong

“…The effects of hnRNPU on cancer progression were assessed through in vitro and in vivo experiments. Gene knockout models and the FTO inhibitor (meclofenamic acid) were used to validate the interaction between hnRNPU and FTO and their impact on AS. …”
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YTHDF2 in peritumoral hepatocytes mediates chemotherapy-induced antitumor immune responses through CX3CL1-mediated CD8+ T cell recruitment by Zhenyun Yang, Xin Wang, Yizhen Fu, Weijie Wu, Zili Hu, Qingyang Lin, Wei Peng, Yangxun Pan, Juncheng Wang, Jinbin Chen, Dandan Hu, Zhongguo Zhou, Li Xu, Yaojun Zhang, Jiajie Hou, Minshan Chen

“…Studies using tumor-bearing liver-specific Ythdf2 knockout mice revealed that hepatocyte YTHDF2 suppresses liver tumor growth through CD8+ T cell recruitment and activation. …”
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Deciphering the potential ability of DExD/H-box helicase 60 (DDX60) on the proliferation, diagnostic and prognostic biomarker in pancreatic cancer: a research based on silico, RNA-... by Dongdong Zhang, Enze Zhang, Ying Cai, Yixin sun, Peiji Zeng, Xiaohua Jiang, Yifan Lian

“…Then MTT assay was performed to explore the effect of DDX60 knockout on the proliferation of pancreatic cancer cells. …”
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Functional genomics implicates ebony in the black pupae phenotype of tephritid fruit flies by Daniel F. Paulo, Thu N. M. Nguyen, Chris M. Ward, Renee L. Corpuz, Angela N. Kauwe, Pedro Rendon, Rocio E. Y. Ruano, Amanda A. S. Cardoso, Georgia Gouvi, Elisabeth Fung, Peter Crisp, Anzu Okada, Amanda Choo, Christian Stauffer, Kostas Bourtzis, Sheina B. Sim, Simon W. Baxter, Scott M. Geib

“…Here, we present evidence from classical and modern genetics showing that the black pupae (bp) phenotype in the GUA10 strain of Anastrepha ludens is caused by a large deletion at the ebony locus, removing the gene’s entire coding region. Targeted knockout of ebony induced analogous bp phenotypes across six major tephritid agricultural pests, demonstrating that disruption of Ebony alone is sufficient to produce the mutant trait in distantly related species. …”
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IL-33 Deficiency Attenuates Lung Inflammation by Inducing Th17 Response and Impacting the Th17/Treg Balance in LPS-Induced ARDS Mice via Dendritic Cells by Li Cheng, Yang Jiao, Wei Jiang, Xin Zhang, Liping Zhang, Gongwei Jia

“…Methods. IL-33 gene knockout mice and the administration of recombinant mouse IL-33 (rmIL-33) were used to investigate the role of IL-33 and the underlying mechanisms in an LPS-induced ARDS model. …”
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Metallothioneins 1 and 2 Modulate Inflammation and Support Remodeling in Ischemic Cardiomyopathy in Mice by Georg D. Duerr, Daniela Dewald, Eva J. Schmitz, Luise Verfuerth, Katharina Keppel, Christine Peigney, Alexander Ghanem, Armin Welz, Oliver Dewald

“…Daily 15-minute LAD-occlusion was performed for 1, 3, and 7 days in SV129 (WT)- and MT1/2 knockout (MT-/-)-mice (n = 8–10/group). Hearts were examined with M-mode echocardiography and processed for histological and mRNA studies. …”
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ZBP1-mediated PANoptosis is a crucial lethal form in diverse keratinocyte death modalities in UVB-induced skin injury by Xuechan Bi, Min Li, Yiming Guo, Mengyao Hu, Yujie Chen, Ni Lian, Sihan Chen, Min Li, Heng Gu, Xu Chen

“…Through keratinocyte-specific gene knockout or using corresponding inhibitors, we found that individual inhibition of GSDMD-mediated pyroptosis, caspase-3-mediated apoptosis, or MLKL-mediated necroptosis did not reduce the overall level of keratinocyte death after UVB exposure, and that the other two pathways maintained the activation. …”
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IL-21 shapes the B cell response in a context-dependent manner by Youngjun Kim, Francesca Manara, Simon Grassmann, Kalina T. Belcheva, Kanelly Reyes, Hyunu Kim, Stephanie Downs-Canner, William T. Yewdell, Joseph C. Sun, Jayanta Chaudhuri

“…Using IL-21 receptor (Il21r) conditional knockout mice and ex vivo culture systems, we demonstrate that IL-21 has dual effects on B cells. …”
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