Deficiency of the palmitoyl acyltransferase ZDHHC7 modulates depression-like behaviour in female mice after a mild chronic stress paradigm by Christa Hohoff, Nicole Kerkenberg, Mingyue Zhang, Weronika Palkowska, Lydia Wachsmuth, Maja Peng, Lena Stiehl, Christiane Schettler, Johannes C. S. Zang, Andreas Huge, Evgeni Ponimaskin, Cornelius Faber, Bernhard T. Baune, Weiqi Zhang

“…Female and male Zdhhc7-knockout (KO) and -wildtype (WT) mice underwent a four-week-mild CS paradigm or non-stress control (C) condition. …”
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MMP9-Associated Tumor Stem Cells, CCL1-Silenced Dendritic Cells, and Cytokine-Induced Killer Cells Have a Remarkable Therapeutic Efficacy for Acute Myeloid Leukemia by Activating T... by Min Dong, Guozhen Zhang, Jie Meng, Biou Liu, Duanfeng Jiang, Feng Liu

“…DC-CIK cells with MMP9 and CCL1 knockout alone had little effect on leukemia cells, while knockdown of MMP9 and CCL1 in DC-CIK cells increased cytotoxicity, suppressed proliferation, and induced apoptosis of leukemia cells. …”
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Consequences of the Lack of CD73 and Prostatic Acid Phosphatase in the Lymphoid Organs by Gennady G. Yegutkin, Kaisa Auvinen, Marika Karikoski, Pia Rantakari, Heidi Gerke, Kati Elima, Mikael Maksimow, Ileana B. Quintero, Pirkko Vihko, Marko Salmi, Sirpa Jalkanen

“…Therefore, we analyzed enzymatic activities and leukocyte subtypes of CD73 and PAP knockouts and generated CD73/PAP double knockout mice to elucidate the contribution of CD73 and PAP to immunological parameters. …”
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A hypomorphic mutation in the mouse Csn1s1 gene generated by CRISPR/Cas9 pronuclear microinjection by A. V. Smirnov, T. A. Shnaider, A. N. Korablev, A. M. Yunusova, I. A. Serova, N. R. Battulin

“…Functionally most mutations led to gene knockout due to a frameshift or a start codon loss. …”
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Cardiomyocyte-Restricted Deletion of PPARβ/δ in PPARα-Null Mice Causes Impaired Mitochondrial Biogenesis and Defense, but No Further Depression of Myocardial Fatty Acid Oxidation... by Jian Liu, Peiyong Wang, Lan He, Yuquan Li, Jinwen Luo, Lihong Cheng, Qianhong Qin, Lawrence A. Brako, Woo-kuen Lo, William Lewis, Qinglin Yang

“…However, previous studies demonstrated that cardiomyocyte-restricted PPARβ/δ deficiency in mice leads to severe cardiac pathological development, whereas global PPARα knockout shows a benign cardiac phenotype. It is unknown whether a PPARα-null background would alter the pathological development in mice with cardiomyocyte-restricted PPARβ/δ deficiency. …”
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A novel anti-epileptogenesis strategy of temporal lobe epilepsy based on nitric oxide donor by Xian-Hui Zhu, Ya-Ping Zhou, Qiao Zhang, Ming-Yi Zhu, Xiao-Wei Song, Jun Li, Jiang Chen, Yun Shi, Kang-Jian Sun, Yong-Jie Zhang, Jing Zhang, Tian Xia, Bao-Sheng Huang, Fan Meng, Qi-Gang Zhou

“…In the present study, Nos1 conditional knockout mice were constructed, and we found that selective deletion of Nos1 in hilar interneurons rather than dentate granular cells (DGCs) triggered epileptogenesis. …”
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Assessing cell lines with inducible depletion of cohesin and condensins components through analysis of metaphase chromosome morphology by A. M. Yunusova, A. V. Smirnov, I. E. Pristyazhnuk, T. A. Shnaider, E. K. Maltseva, S. D.  Afonnikova, O. A. Gusev, N.  R. Battulin

“…For this purpose, cells or organisms with a knockout of the gene encoding the protein of interest are obtained. …”
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The guanine nucleotide exchange factor DOCK5 negatively regulates osteoblast differentiation and BMP2-induced bone regeneration via the MKK3/6 and p38 signaling pathways by Ju Ang Kim, Soomin Im, Jiwon Lim, Jung Min Hong, Hye Jung Ihn, Jong-Sup Bae, Jung-Eun Kim, Yong Chul Bae, Eui Kyun Park

“…C21 significantly enhanced osteoblast differentiation and mineral deposition in mouse MC3T3-E1 cells and in human and mouse BMSCs. Dock5 knockout (KO) mice exhibited increased bone mass and mineral apposition rate, with their BMSCs showing enhanced osteoblast differentiation. …”
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Effects of genetic ablation and pharmacological inhibition of HuR on gene expression, iron metabolism, and hormone levels by Nathalie Idlin, Sivakumar Krishnamoorthy, Magdalena Wolczyk, Mouad Fakhri, Michal Lechowski, Natalia Stec, Jacek Milek, Pratik Kumar Mandal, Jaroslaw Cendrowski, Christos Spanos, Magdalena Dziembowska, Katarzyna Mleczko-Sanecka, Juri Rappsilber, Gracjan Michlewski

“…We show that both HuR knockout and Eltrombopag treatment modulate iron metabolism by decreasing ferritin heavy chain (FTH1) and light chain (FTL) synthesis while increasing the expression of iron-regulatory protein 2 (IRP2), a key regulator of ferritin translation. …”
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Induced Pluripotent Stem Cell Derivation and Ex Vivo Gene Correction Using a Mucopolysaccharidosis Type 1 Disease Mouse Model by Toshio Miki, Ludivina Vazquez, Lisa Yanuaria, Omar Lopez, Irving M. Garcia, Kazuo Ohashi, Natalie S. Rodriguez

“…Disease-affected iPSCs were generated from Idua knockout mouse embryonic fibroblasts, which carry a disrupting neomycin-resistant gene cassette (Neor) in exon VI of the Idua gene. …”
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Invasion of pancreatic ductal epithelial cells by Enterococcus faecalis is mediated by fibronectin and enterococcal fibronectin-binding protein A by Munefumi Shimosaka, Jumpei Kondo, Mamika Sonoda, Rui Kawaguchi, Emika Noda, Kaho Nishikori, Asuka Ogata, Shinji Takamatsu, Ken Sasai, Hirofumi Akita, Hidetoshi Eguchi, Yoshihiro Kamada, Shigefumi Okamoto, Eiji Miyoshi

“…Mechanistically, bacterial expression of enterococcal fibronectin-binding protein A (EfbA) was correlated with adhesive potential of E. faecalis strains. Knockout of fibronectin 1, a binding partner of EfbA, in iPDECs resulted in suppressed E. faecalis adhesion and invasion, suggesting the importance of the EfbA-fibronectin axis in infection of pancreatic ductal epithelial lineage cells. …”
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Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization by Junpei Fukumoto, Minako Yoshida, Suzumi M. Tokuoka, Eri Saki H. Hayakawa, Shinya Miyazaki, Takaya Sakura, Daniel Ken Inaoka, Kiyoshi Kita, Jiro Usukura, Hideo Shindou, Fuyuki Tokumasu

“…Mass spectrometry-based enzyme assay and lipidomic analysis demonstrate that recombinant PfLPLAT1 exhibits lysophospholipid acyltransferase activity with a preference for unsaturated fatty acids as its acyl donors and lysophosphatidic acids as an acceptor, with its conditional knockout leading to abnormal lipid composition and marked morphological and developmental changes including stage arrest. …”
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IL-17 Is a Key Regulator of Mucin-Galectin-3 Interactions in Asthma by Manoj J. Mammen, Jamil Ali, Amita Aurora, Umesh C. Sharma, Ravikumar Aalinkeel, Supriya D. Mahajan, Mark Sands, Stanley A. Schwartz

“…We previously showed that the absence of tissue inhibitor of metalloproteinase 1 (TIMP-1) enhances lung inflammation, airway hyperreactivity, and lung remodeling in asthma in an ovalbumin (OVA) asthma model of TIMP-1 knockout (TIMPKO) mice as compared to wild-type (WT) controls and mediated by increased galectin-3 (Gal-3) levels. …”
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Betacoronaviruses Differentially Activate the Integrated Stress Response to Optimize Viral Replication in Lung-Derived Cell Lines by David M. Renner, Nicholas A. Parenti, Nicole Bracci, Susan R. Weiss

“…Interestingly, genetic ablation of growth arrest and DNA damage-inducible protein (GADD34) expression, an inducible protein phosphatase 1 (PP1)-interacting partner targeting eIF2α for dephosphorylation, did not significantly alter HCoV-OC43 or SARS-CoV-2 replication, while siRNA knockdown of the constitutive PP1 partner, constitutive repressor of eIF2α phosphorylation (CReP), dramatically reduced HCoV-OC43 replication. Combining GADD34 knockout with CReP knockdown had the maximum impact on HCoV-OC43 replication, while SARS-CoV-2 replication was unaffected. …”
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Phenylbutyric Acid Modulates Apoptosis and ER Stress‐Related Gene Expression in Glycogen Storage Disease Type Ib In Vitro Model by Marina Parezanovic, Nina Stevanovic, Marina Andjelkovic, Milena Ugrin, Sonja Pavlovic, Maja Stojiljkovic, Anita Skakic

“…Methods G6PT‐deficient FlpInHEK293 cell line was created and validated using the CRISPR/Cas9 knockout method. Molecular markers of unfolded protein response (ATF4, DDIT3, HSPA5, XBP1s), and apoptosis (BCL2/BAX, CASP3, CASP7) in G6PT‐deficient cells were analyzed using RT‐qPCR method before and upon the treatment with 4‐PBA. …”
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The regulatory effects of (p)ppGpp and indole on cAMP synthesis in <i>Escherichia coli</i> cells by N. M. Kashevarova, E. A. Khaova, A. G. Tkachenko

“…E. coli parent strain BW25141 ((p)ppGpp+) and deletion knockout BW25141ΔrelAΔspoT which is unable to synthesize (p)ppGpp ((p)ppGpp0) were cultivated in M9 medium supplemented with different glucose concentrations (5.6 and 22.2 mM) in the presence of tryptophan as a substrate for indole synthesis and in its absence. …”
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A Novel Mouse Model of Alzheimer's Disease with Chronic Estrogen Deficiency Leads to Glial Cell Activation and Hypertrophy by Annik Prat, Maik Behrendt, Edwige Marcinkiewicz, Sebastien Boridy, Ram M. Sairam, Nabil G. Seidah, Dusica Maysinger

“…These chimeric mice exhibit a total FSH-R knockout (FORKO) and carry two transgenes, one expressing the β-amyloid precursor protein (APPsw, Swedish mutation) and the other expressing presenilin-1 lacking exon 9 (PS1Δ9). …”
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Significant Contribution of Mouse Mast Cell Protease 4 in Early Phases of Experimental Autoimmune Encephalomyelitis by Louisane Desbiens, Catherine Lapointe, Marjan Gharagozloo, Shaimaa Mahmoud, Gunnar Pejler, Denis Gris, Pedro D’Orléans-Juste

“…C57BL/6 wild type (WT) or mMCP-4 knockout (KO) mice were immunized with MOG35–55 plus complete Freund’s adjuvant followed by pertussis toxin. …”
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Comprehensive Pan-Cancer Analysis and Functional Studies Reveal SLC2A6 as a Ferroptosis Modulator in Hepatocellular carcinoma by Guohong Yan, Hailian Huang, Ziyan Lu, Meifeng Chen, Xiang Wang, Pei Zhong, Chongjiu Qin, Shutian Mo, Chuangye Han, Xiaoling Luo, Xinping Ye

“…Furthermore, in vitro and in vivo experiments were performed to assess the impact of SLC2A6 knockout on the proliferation, migration, invasion, and underlying mechanisms in hepatocellular carcinoma (LIHC) cells. …”
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The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma by Shahid Banday, Alok K. Mishra, Romana Rashid, Tianyi Ye, Amjad Ali, Junhui Li, Jason T. Yustein, Michelle A. Kelliher, Lihua Julie Zhu, Sara K. Deibler, Sunil K. Malonia, Michael R. Green

“…Here, using genome-scale CRISPR/Cas9 knockout screening, we identify C1GALT1, a galactosyltransferase required for the biosynthesis of many O-glycoproteins, as a factor that promotes EWSR1::FLI1 expression. …”
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