Beclin 1-Mediated Autophagy Is Potentiated by an Interaction with the Neuronal Adaptor FE65 by Wai Wa Ray Chan, Jessica Chow, Dennis Dik-Long Chau, Yuqi Zhai, Kwok-Fai Lau

“…Notably, the overexpression of FE65 enhances Beclin 1-mediated autophagy, whereas this process is attenuated in FE65 knockout cells. Moreover, the stimulatory effect of FE65 on Beclin 1-mediated autophagy is diminished by an FE65 C-terminus deletion mutant that disrupts the FE65–Beclin 1 interaction. …”
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CRYβB2 alters cell adhesion to promote invasion in a triple-negative breast cancer cell line by Amr A. Waly, London Harper, Jodie M. Fleming, Lindsey M. Costantini

“…Specifically, CRYβB2 suppressed the expression of key cell-cell adhesion genes known as clustered protocadherins and promoted the expression of PCDH7, a nonclustered protocadherin with known oncogenic roles in various cancers. Notably, the knockout of PCDH7 diminished the invasive capacity induced by CRYβB2 (median invasion %; SUM159 = 0.093, SUM159 + CRYβB2 = 0.184 and SUM159 + CRYβB2/PCDH7−/−=0.082). …”
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In vitro sperm generation from immature mouse testicular tissue using plasma rich in growth factors by Seyyed Amir Moradian, Mansoureh Movahedin

“…Abstract Background Culture medium enriched with Knockout serum replacement (KSR) can produce in vitro mouse sperm, but it is inefficient, strain-specific and contains bovine products, which limits its use in the human clinic. …”
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Loss of BRCA1 Spontaneously Induces the Tumorigenesis in Lacrimal Gland by Sun Eui Kim, Hye Jung Baek, Eun Jung Park, Sung Chul Lim, Sang Soo Kim

“…Female Brca1-mutant mice (Brca1co/coMMTV-cre) were generated by crossing Brca1 conditional knockout mice and MMTV-cre mice, and the occurrence of lacrimal gland abnormalities and tumors was followed until mice reached 18 months of age. …”
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SOD1 Is Essential for the Viability of DT40 Cells and Nuclear SOD1 Functions as a Guardian of Genomic DNA by Eri Inoue, Keizo Tano, Hanako Yoshii, Jun Nakamura, Shusuke Tada, Masami Watanabe, Masayuki Seki, Takemi Enomoto

“…We generated conditional SOD1 knockout cells from chicken DT40 cells and analyzed their phenotypes. …”
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Mettl3-m6A-NPY axis governing neuron–microglia interaction regulates sleep amount of mice by Qihang Sun, Jinpiao Zhu, Xingsen Zhao, Xiaoli Huang, Wenzheng Qu, Xia Tang, Daqing Ma, Qiang Shu, Xuekun Li

“…We also showed that Mettl3 regulates neuropeptide Y (NPY) via m6A modification and Mettl3 conditional knockout (cKO) mice displayed significantly decreased expression of NPY in thalamus. …”
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Siglec-E augments adipose tissue inflammation by modulating TRAF3 signaling and monocytic myeloid-derived suppressor cells during obesity by Ahmed Rakib, Mousumi Mandal, Md Abdullah Al Mamun, Sonia Kiran, Nelufar Yasmen, Lexiao Li, Daniel M. Collier, Jianxiong Jiang, Frank Park, Udai P. Singh

“…RNA-Seq analysis of AT immune cells isolated from mice fed a high-fat diet (HFD) versus normal fat diet (ND) identified a panel of genes that were markedly downregulated, including sialic acid-binding Ig-like lectin E (siglec-E), in HFD compared to ND mice.MethodsA series of experiments in wild-type (WT) and siglec-E knockout (siglec-E KO) mice was designed to investigate the effect of HFD on the functional role of siglec-E in the regulation of AT inflammation and adipogenesis. …”
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Adiponectin Deficiency Blunts Hypoxia-Induced Mobilization and Homing of Circulating Angiogenic Cells by Bert R. Everaert, Vincent J. Nijenhuis, Florence C. M. Reith, Vicky Y. Hoymans, Jean-Pierre Timmermans, Christiaan J. Vrints

“…AMI was induced in wild-type (WT) (n=10) and adiponectin knockout (Adipoq−/−) mice (n=7). One week after AMI, bone marrow (BM) concentration and mobilization of Sca-1+ and Lin−Sca-1+ progenitor cells (PCs) were markedly attenuated under Adipoq−/− conditions, as assessed by flow cytometry. …”
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Nuclear Factor I-C Regulates Stemness Genes and Proliferation of Stem Cells in Various Mineralized Tissue through Epithelial-Mesenchymal Interactions in Dental Epithelial Stem Cell... by Dong-Seol Lee, Yeo Joon Song, Hye Ri Gug, Ji-Hyun Lee, Hyun Sook Bae, Joo-Cheol Park

“…Among its members, nuclear factor I-C (NFI-C) has been reported to play an essential role in odontogenesis. Nfic knockout mice show malformation in all mineralized tissues, but it remains unclear which stage of development Nfic is involved in. …”
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NRF2 Exerts Anti-Inflammatory Effects in LPS-Induced gEECs by Inhibiting the Activation of the NF-κB by Hongchu Bao, Qinglan Qu, Wei Zhang, Xinrong Wang, Jianye Fang, Jinwen Xue, Zhenteng Liu, Shunzhi He

“…LPS was found to increase the NRF2 expression and the nuclear abundance of NRF2 in gEECs in a dose-dependent manner. NRF2 knockout (KO) not only increased the expression of LPS-induced proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-8) but also increased the expression of TLR4, p-IκBα/IκBα, and p-p65/p65 proteins. …”
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Mechanism of hyperbaric oxygen therapy downregulating H-type angiogenesis in subchondral bone of knee osteoarthritis through the PHD2/HIF-1α pathway by Jianjian Wang, Wen Yu, Yuxin Zhang, Bo Chen, Zhaoxiang Meng

“…The activation relationship between PHD2 and downstream signaling pathways was investigated through gene knockout and overexpression experiments. Finally, cell scratch assays, tube formation assays, and chondrogenic differentiation experiments were conducted to verify the mechanism of the PHD2/HIF-1α signaling pathway under hyperbaric oxygen stimulation. …”
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NupR Is Involved in the Control of PlcR: A Pleiotropic Regulator of Extracellular Virulence Factors by Jiaxin Qin, Ziqi Wang, Cheng Qian, Guohui Ji, Yizhuo Zhang, Zhanglei Cao, Bing Yan, Jun Cai

“…In addition, NupR regulates the expression of the PlcR regulon, including hemolysin, phospholipase C, <i>papR</i>, and oligopeptide permease, which could result in the culture supernatant of BMB171 being less virulent to sf9 cells compared to the <i>nupR</i> knockout strain. The results combine the nutritional status of cells with virulence to form a regulatory loop, providing new ideas and research foundations for the study of bacterial virulence.…”
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Network analyses of brain tumor multiomic data reveal pharmacological opportunities to alter cell state transitions by Brandon Bumbaca, Jonah R. Huggins, Marc R. Birtwistle, James M. Gallo

“…These four-cell state PPINs were pooled to form a single Boolean network that was used for in silico protein knockout simulations to investigate mechanisms that either promote or prevent cell state transitions. …”
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Wogonin effects on the efflux transporters BCRP and MRP2, explain its effectiveness in ulcerative colitis: Implications for metabolic and transport interactions by Yuanyuan Liu, Zerong Zhang, Xiaoyan Li, Qinghong Hu, Zhangyu Jiang, Jia Lv, Jiayi Xue, Dongyu Wang, Jianxiong Cao, Lingyu Li, Xiaowen Ou, Lijun Zhu, Zhongqiu Liu, Tao Su

“…Moreover, in vitro results showed that after knockout of BCRP and MRP2, the concentration of wogonin increased, and its UGT metabolite wogonoside decreased in both cells and mitochondria. …”
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Allelic differences in the key genes of betalain biosynthesis in table beet accessions with contrasting root color from the VIR collection by A. S. Mikhailova, D. V. Sokolova, N. A. Shvachko, V. S. Popov, E. K. Khlestkina

“…Attention should be paid to these copies, together with the CYP76AD6 gene, as they seem the most preferable targets for a knockout to increase the red pigment content.Conclusion. …”
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Prioritization of genes associated with the pathogenesis of leukosis in cattle by N. S. Yudin, N. L. Podkolodnyy, T. A. Agarkova, E. V. Ignatieva

“…The following criteria were used to prioritize 446 genes from the catalog: (1) the gene is associated with leukemia according to a genome-wide association study; (2) the gene is associated with leukemia according to a case-control study; (3) the role of the gene in leukemia development has been studied using knockout mice; (4) protein-protein interactions exist between the gene-encoded protein and either viral particles or individual viral proteins; (5) the gene is annotated with Gene Ontology terms that are overrepresented for a given list of genes; (6) the gene participates in biological pathways from the KEGG or REACTOME databases, which are over-represented for a given list of genes; (7) the protein encoded by the gene has a high number of protein-protein interactions with proteins encoded by other genes from the catalog. …”
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Maturation and detoxification of synphilin-1 inclusion bodies regulated by sphingolipids by Xiuling Cao, Xiang Wu, Lei Zhao, Ju Zheng, Xuejiao Jin, Xinxin Hao, Joris Winderickx, Shenkui Liu, Lihua Chen, Beidong Liu

“…Pharmacological inhibition of the sphingolipid metabolism pathway or knockout of its key genes results in a delayed IB maturation and increased SY1 cytotoxicity. …”
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Downmodulation of Vaccine-Induced Immunity and Protection against the Intracellular Bacterium Francisella tularensis by the Inhibitory Receptor FcγRIIB by Brian J. Franz, Ying Li, Constantine Bitsaktsis, Bibiana V. Iglesias, Giang Pham, Raju Sunagar, Sudeep Kumar, Edmund J. Gosselin

“…Naïve and iFt-immunized FcγRIIB knockout (KO) or wildtype (WT) mice were challenged with Ft-live vaccine strain (LVS). …”
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MCU expression in hippocampal CA2 neurons modulates dendritic mitochondrial morphology and synaptic plasticity by Katy E. Pannoni, Quentin S. Fischer, Renesa Tarannum, Mikel L. Cawley, Mayd M. Alsalman, Nicole Acosta, Chisom Ezigbo, Daniela V. Gil, Logan A. Campbell, Shannon Farris

“…Using a CA2-specific MCU knockout (cKO) mouse, we found that MCU deletion impairs plasticity at distal dendrite synapses. …”
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Transcriptomic Analysis Reveals an Altered Hcy Metabolism in the Stria Vascularis of the Pendred Syndrome Mouse Model by Wenyue Xue, Yuxin Tian, Yuanping Xiong, Feng Liu, Yanmei Feng, Zhengnong Chen, Dongzhen Yu, Shankai Yin

“…The altered expression of Bhmt results in a dramatic change in multiple biochemical reactions and a disruption of nutrient homeostasis in the endolymph which may contribute to hearing loss of Slc26a4 knockout mouse.…”
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