Defective Slc7a7 transport reduces erythropoietin compromising erythropoiesis by Judith Giroud-Gerbetant, Fernando Sotillo, Gonzalo Hernández, Irene Ruano, David Sebastián, Joana Fort, Mayka Sánchez, Günter Weiss, Neus Prats, Antonio Zorzano, Manuel Palacín, Susanna Bodoy

“…Methods We employed total inducible and cell type-specific Slc7a7 knockout mouse models to determine whether the hematological abnormalities observed in LPI are due to the loss of Slc7a7 function in hematopoietic cells. …”
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Gene function revealed at the moment of stochastic gene silencing by Shreyan Gupta, James J. Cai

“…Analysis of real data demonstrates that scSGS can reveal regulatory relationships up- and downstream of target genes, circumventing the survivorship bias that often affects gene knockout and perturbation studies. scSGS thus offers an efficient approach for gene function prediction, with significant potential to reduce the use of genetically modified animals in gene function research.…”
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DYRK1A (Dual-Specificity Tyrosine-Phosphorylated and -Regulated Kinase 1A): A Gene with Dosage Effect During Development and Neurogenesis by M. Dierssen, M. Martínez de Lagrán

“…In Drosophila, disruption of the homolog minibrain gene results in flies with reduced neuroblast proliferation, decreased numbers of central brain neurons, and learning/memory deficits. Knockout DYRK1A mice are embryonic lethal, and heterozygotes show decreased viability and region-specific reductions in brain size. …”
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SCRAPPER Regulates the Thresholds of Long-Term Potentiation/Depression, the Bidirectional Synaptic Plasticity in Hippocampal CA3-CA1 Synapses by Hiroshi Takagi, Mitsutoshi Setou, Seiji Ito, Ikuko Yao

“…Compared to wild-type mice, Scrapper-knockout mice exhibited LTDs with smaller magnitudes after induction with low-frequency stimulation and LTPs with larger magnitudes after induction with tetanus stimulation. …”
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FGF21 as an Endocrine Regulator in Lipid Metabolism: From Molecular Evolution to Physiology and Pathophysiology by Yusuke Murata, Morichika Konishi, Nobuyuki Itoh

“…However, experiments with Fgf21 knockout mice indicate that FGF21 inhibits lipolysis in adipocytes during fasting and attenuates torpor induced by a ketogenic diet but maybe not a physiological regulator for these hepatic functions. …”
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CAMSAP2 is required for bridging fiber assembly to ensure mitotic spindle assembly and chromosome segregation in human epithelial Caco-2 cells. by Naoko Nishizawa, Riku Arai, Koki Hiranuma, Mika Toya, Masamitsu Sato

“…Here, we demonstrate that CAMSAP2, but not CAMSAP3, is required for spindle assembly upon mitotic entry. CAMSAP2 knockout (KO) Caco-2 cells showed a delay in mitotic progression, whereas CAMSAP3 KO cells did not. …”
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Hemoglobin: A Nitric-Oxide Dioxygenase by Paul R. Gardner

“…Unraveling the physiological functions of multiple NODs with varying expression in organisms and the complexity of NO as both a poison and signaling molecule remain grand challenges for the NO field. NOD knockout organisms and cells expressing recombinant NODs are helping to advance our understanding of NO actions in microbial infection, plant senescence, cancer, mitochondrial function, iron metabolism, and tissue O2 homeostasis. …”
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CD4 expression controls epidermal stem cell balance by Nadine Brandes, Heidi Hahn, Anja Uhmann

“…Wound healing studies furthermore show that the progeny of CD4+ epidermal cells accumulates at wound sites. Finally, using CD4 knockout mice we demonstrate that CD4 expression is essential for maintaining fast-cycling epidermal stem cells during homeostasis and that CD4 loss mitigates the age-related decline in wound repair capacity. …”
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Beam stacking experiment at a fixed field alternating gradient accelerator by T. Uesugi, Y. Ishi, Y. Kuriyama, Y. Mori, C. Jolly, D. J. Kelliher, J.-B. Lagrange, A. P. Letchford, S. Machida, D. W. Posthuma de Boer, C. T. Rogers, E. Yamakawa, M. Topp-Mugglestone

“…The intensity of the first beam was, however, significantly reduced. rf knockout is the suspected source of the beam loss.…”
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CPEB4 modulates liver cancer progression by translationally regulating hepcidin expression and sensitivity to ferroptosis by M. Eugenia Delgado, Salvador Naranjo-Suarez, Marta Ramírez-Pedraza, Beatriz I. Cárdenas, Carmen Gallardo-Martínez, Alexandra Balvey, Eulalia Belloc, Judit Martín, Mark Boyle, Raúl Méndez, Mercedes Fernandez

“…In mouse models of diet-induced liver cancer (n = 10–15 per group), both systemic and hepatocyte-specific CPEB4 knockout mice exhibited significantly increased tumor burden compared with wild-type controls (p <0.05). …”
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Disulfidptosis-related gene signatures as prognostic biomarkers and predictors of immunotherapy response in HNSCC by Haotian Qin, Haotian Qin, Juan Xu, Yaohang Yue, Yaohang Yue, Meiling Chen, Zheng Zhang, Panpan Xu, Yan Zheng, Hui Zeng, Jian Weng, Jian Weng, Jun Yang, Fei Yu

“…DSTN upregulation promotes tumor growth, and its knockout inhibits proliferation, migration, and invasion.…”
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Telomere Length Is Associated With Adverse Atrial Remodeling in Patients With Atrial Fibrillation by Kabita Pradhan, Balram Neupane, Paul Niehues, Martin Kirschner, Fabian Beier, Chao‐Chung Kuo, Erika Anneliese Hilbold, Christian Bär, Oana‐Maria Thoma, Maximilian Waldner, Margherita Vieri, Tim H. Brümmendorf, Vithurithra Tharmapalan, Wolfgang Wagner, Michael Kleines, Mahdi Emrani, Matthias Daniel Zink, Andreas Napp, Nikolaus Marx, Michael Gramlich

“…Telomerase reverse transcriptase knockout (Tert−/−) and telomerase RNA component knockout (Terc−/−) mice were used to investigate the mechanistic impact of telomere shortening on atrial remodeling. …”
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Fhl1, a new spatially specific protein, regulates vein graft neointimal hyperplasia by Chaoqun Wang, Jiantao Chen, Zicong Feng, Bohao Jian, Suiqing Huang, Kangni Feng, Haoliang Liu, Zhuoming Zhou, Ziyin Ye, Jing Lu, Mengya Liang, Zhongkai Wu

“…Immunohistochemical analysis identified neointima‐specific markers, while NIH progression was assessed in SD rats with four and a half LIM domains protein 1 (Fhl1) knockout and in human saphenous veins (HSV) with adenovirus‐mediated Fhl1 overexpression. …”
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ATP1B3 may promote glioma proliferation and migration through MAPK/NF-KB signaling pathway by Qikang Yan, Quan Sun, Yan Feng, Qingyun Hu, Jinling Zhu

“…The mRNA and protein expression levels of ATP1B3 were significantly decreased after knockout, and the proliferation, migration and invasion ability of cells in knockout group were significantly lower than those in control group, with statistical difference. …”
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Tendon Stem/Progenitor Cells and Their Interactions with Extracellular Matrix and Mechanical Loading by Chuanxin Zhang, Jun Zhu, Yiqin Zhou, Bhavani P. Thampatty, James H-C. Wang

“…Finally, future research efforts are suggested, which include using specific gene knockout models and single-cell transcription profiling to enable a broad and deep understanding of the physiology and pathophysiology of tendons.…”
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LOX-1, OxLDL, and Atherosclerosis by Angela Pirillo, Giuseppe Danilo Norata, Alberico Luigi Catapano

“…The key contribution of LOX-1 to the atherogenic process has been confirmed in animal models; LOX-1 knockout mice exhibit reduced intima thickness and inflammation and increased expression of protective factors; on the contrary, LOX-1 overexpressing mice present an accelerated atherosclerotic lesion formation which is associated with increased inflammation. …”
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Tubulin tyrosination/detyrosination regulate the affinity and sorting of intraflagellar transport trains on axonemal microtubule doublets by Aditya Chhatre, Ludek Stepanek, Adrian Pascal Nievergelt, Gonzalo Alvarez Viar, Stefan Diez, Gaia Pigino

“…Here, we test whether tubulin detyrosination (enriched on B-tubules) and tyrosination (enriched on A-tubules) have a role in IFT logistics. We report that knockout of tubulin detyrosinase VashL in Chlamydomonas reinhardtii causes frequent IFT train stoppages and impaired ciliary growth. …”
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Peroxisome Proliferator-Activated Receptor α Plays an Important Role in the Expression of Monocyte Chemoattractant Protein-1 and Neointimal Hyperplasia after Vascular Injury by Yu Peng, Qiang Li, Lu Zhang, Ming Bai, Zheng Zhang

“…Carotid artery injury was performed in the PPARα knockout and wild type (WT) mice, treated and untreated with Wy14643, a PPARα activator. …”
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Cell-specific functions of androgen receptor in skeletal muscles by Hiroshi Sakai, Yuuki Imai

“…Drawing from insights gained using conditional knockout mouse models facilitated by Cre/loxP technology, we review the cell-specific functions of AR in skeletal muscles. …”
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The role of oxidative stress, glucocorticoid receptor and ARMC5 in lipid metabolism by Yosuke Okuno, Atsunori Fukuhara, Iichiro Shimomura

“…Using adipocyte-specific glucocorticoid receptor knockout mice, we found that glucocorticoids also inhibited healthy adipose expansion and decreased insulin sensitivity. …”
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