Cell Reprogramming, IPS Limitations, and Overcoming Strategies in Dental Bioengineering by Gaskon Ibarretxe, Antonia Alvarez, Maria-Luz Cañavate, Enrique Hilario, Maitane Aurrekoetxea, Fernando Unda

“…The procurement of induced pluripotent stem cells, or IPS cells, from adult differentiated animal cells has the potential to revolutionize future medicine, where reprogrammed IPS cells may be used to repair disease-affected tissues on demand. …”
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zIncubascope: Long-term quantitative imaging of multi-cellular assemblies inside an incubator. by Anirban Jana, Naveen Mekhileri, Adeline Boyreau, Aymerick Bazin, Nadège Pujol, Kevin Alessandri, Gaëlle Recher, Pierre Nassoy, Amaury Badon

“…We exemplify the capabilities of our system by investigating human induced pluripotent stem cells (hiPSCs) enclosed in spherical capsules, hiPSCs in tubular capsules and yeast cells in spherical capsules. …”
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Cholangiocyte organoids for disease, cancer, and regenerative medicine by Munemasa Nagao, Akihisa Fukuda, Hirotaka Kashima, Sho Matsuyama, Kei Iimori, Shinnosuke Nakayama, Kenta Mizukoshi, Munenori Kawai, Go Yamakawa, Mayuki Omatsu, Mio Namikawa, Tomonori Masuda, Yukiko Hiramatsu, Yu Muta, Takahisa Maruno, Yuki Nakanishi, Tatsuaki Tsuruyama, Hiroshi Seno

“…With advancements in 3D culture technology, cholangiocyte organoids (COs) derived from primary tissues or induced pluripotent stem cells (iPSCs) can accurately replicate the structural and functional properties of biliary tissues. …”
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Current Bioengineering Methods for Whole Kidney Regeneration by Shuichiro Yamanaka, Takashi Yokoo

“…Here, we review recent advances in the field of kidney regeneration, including (i) the directed differentiation of induced pluripotent stem cells/embryonic stem cells into kidney cells; (ii) blastocyst decomplementation; (iii) use of a decellularized cadaveric scaffold; (iv) embryonic organ transplantation; and (v) use of a nephrogenic niche for growing xenoembryos for de novo kidney regeneration from stem cells. …”
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Unchain My Heart: Integrins at the Basis of iPSC Cardiomyocyte Differentiation by Rosaria Santoro, Gianluca Lorenzo Perrucci, Aoife Gowran, Giulio Pompilio

“…In particular, mechanotransduction is of growing importance in groundbreaking cellular models such as induced pluripotent stem cells (iPSC), since this process may strongly influence cell fate and, thus, augment the precision of differentiation into specific cell types, e.g., cardiomyocytes. …”
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Protein Kinases and Their Inhibitors in Pluripotent Stem Cell Fate Regulation by Jungwoon Lee, Young-Jun Park, Haiyoung Jung

“…Furthermore, new reprogramming technologies have been developed using kinase modulators, which have provided novel insight of the mechanisms underlying the kinase regulatory networks involved in the generation of induced pluripotent stem cells (iPSCs). In this review, we highlight the recent progress made in defining the roles of protein kinase signaling pathways and their small molecule modulators in regulating the pluripotent states, self-renewal, reprogramming process, and lineage differentiation of PSCs.…”
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Emerging Stem Cell Therapies: Treatment, Safety, and Biology by Joel Sng, Thomas Lufkin

“…The discovery of induced pluripotent stem cells has led to an improved understanding of the underlying epigenetic keys of pluripotency and carcinogenesis. …”
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A synthetic chronogenetic gene circuit for programmed circadian drug delivery by Lara Pferdehirt, Anna R. Damato, Kristin L. Lenz, Maria F. Gonzalez-Aponte, Daniel Palmer, Qing-Jun Meng, Erik D. Herzog, Farshid Guilak

“…Gene circuits were transduced into induced pluripotent stem cells that were tissue-engineered into cartilage constructs. …”
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Current Therapeutic Strategies for Stem Cell-Based Cartilage Regeneration by Yoojun Nam, Yeri Alice Rim, Jennifer Lee, Ji Hyeon Ju

“…We also review the chondrogenic potential of cells according to their origin, including autologous chondrocytes, mesenchymal stem cells, and induced pluripotent stem cells.…”
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Highly efficient construction of monkey blastoid capsules from aged somatic cells by Junmo Wu, Tianao Shao, Zengli Tang, Gaojing Liu, Zhuoyao Li, Yuxi Shi, Yu Kang, Jiawei Zuo, Bo Zhao, Guangyu Hu, Jiaqi Liu, Weizhi Ji, Lei Zhang, Yuyu Niu

“…In this work, we demonstrate the efficient generation of blastoids from induced pluripotent stem cells and somatic cell nuclear transfer embryonic stem cells derived from aged monkeys, achieving an 80% formation efficiency. …”
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Regenerative Therapy of Type 1 Diabetes Mellitus: From Pancreatic Islet Transplantation to Mesenchymal Stem Cells by Nadine E. Rekittke, Meidjie Ang, Divya Rawat, Rahul Khatri, Thomas Linn

“…MSCs are recognized to meet the demand of an individual therapy not raising the concerns of embryonic or induced pluripotent stem cells for therapy.…”
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Mesenchymal Stem and Progenitor Cells in Regeneration: Tissue Specificity and Regenerative Potential by Rokhsareh Rohban, Thomas Rudolf Pieber

“…The potential use of embryonic stem cells and invention of induced pluripotent stem cells raised hope for clinical regenerative purposes; however, the use of these interventions for regenerative therapy showed its dark side, as many health concerns and ethical issues arose in terms of using these cells in clinical applications. …”
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Long Noncoding RNA Regulation of Pluripotency by Alessandro Rosa, Monica Ballarino

“…As such, human Embryonic Stem Cells (hESCs) and human induced Pluripotent Stem Cells (hiPSCs) provide a unique opportunity for studying the earliest steps of human embryogenesis and, at the same time, are of great therapeutic interest. …”
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Atlas of multilineage stem cell differentiation reveals TMEM88 as a developmental regulator of blood pressure by Sophie Shen, Tessa Werner, Samuel W. Lukowski, Stacey Andersen, Yuliangzi Sun, Woo Jun Shim, Dalia Mizikovsky, Sakurako Kobayashi, Jennifer Outhwaite, Han Sheng Chiu, Xiaoli Chen, Gavin Chapman, Ella M. M. A. Martin, Di Xia, Duy Pham, Zezhuo Su, Daniel Kim, Pengyi Yang, Men Chee Tan, Enakshi Sinniah, Qiongyi Zhao, Sumedha Negi, Meredith A. Redd, Joseph E. Powell, Sally L. Dunwoodie, Patrick P. L. Tam, Mikael Bodén, Joshua W. K. Ho, Quan Nguyen, Nathan J. Palpant

“…Here, we engineer barcoded induced pluripotent stem cells to generate an atlas of multilineage differentiation from pluripotency, encompassing an eight-day time course with modulation of WNT, BMP, and VEGF signalling pathways. …”
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Autophagy in Stem Cell Biology: A Perspective on Stem Cell Self-Renewal and Differentiation by Xihang Chen, Yunfan He, Feng Lu

“…In this review, we describe the most significant advances in the understanding of autophagy regulation in hematopoietic and mesenchymal stem cells, as well as in induced pluripotent stem cells. In particular, we highlight the roles of various autophagy activities in the regulation of self-renewal and differentiation of these stem cells.…”
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Dysregulation of REST and its target genes impacts the fate of neural progenitor cells in down syndrome by Tan Huang, Sharida Fakurazi, Pike-See Cheah, King-Hwa Ling

“…Furthermore, we identified nuclear REST loss and the neuroblast marker, DCX, was downregulated in DS human trisomic induced pluripotent stem cells (hiPSCs)-derived NPCs, whereas the glioblast marker, NFIA, was upregulated. …”
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Phospho-seq: integrated, multi-modal profiling of intracellular protein dynamics in single cells by John D. Blair, Austin Hartman, Fides Zenk, Philipp Wahle, Giovanna Brancati, Carol Dalgarno, Barbara Treutlein, Rahul Satija

“…We apply our workflow to cell lines, induced pluripotent stem cells, and months-old retinal and brain organoids to demonstrate its broad applicability. …”
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Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components by Malena Herrera Lopez, Matías Bertone Arolfo, Mónica Remedi, Laura Gastaldi, Carlos Wilson, Gonzalo G. Guendulain, Danilo Ceschin, Andrés Cardozo Gizzi, Alfredo Cáceres, Ana Lis Moyano

“…Human neural rosettes (hNRs) are radial cell structures that assemble from induced pluripotent stem cells (hiPSCs) and recapitulate some stages of neural tube morphogenesis. …”
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Engineering synthetic signaling receptors to enable erythropoietin-free erythropoiesis by Aadit P. Shah, Kiran R. Majeti, Freja K. Ekman, Sridhar Selvaraj, Devesh Sharma, Roshani Sinha, Eric Soupene, Prathamesh Chati, Sofia E. Luna, Carsten T. Charlesworth, Travis McCreary, Benjamin J. Lesch, Tammy Tran, Simon N. Chu, Matthew H. Porteus, M. Kyle Cromer

“…By integrating synEPOR at the endogenous EPOR locus in O-negative induced pluripotent stem cells, we achieve equivalent erythroid differentiation, transcriptomic changes, and hemoglobin production using small molecules compared to EPO-supplemented cultures. …”
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Epigenetic Research of Neurodegenerative Disorders Using Patient iPSC-Based Models by Rubén Fernández-Santiago, Mario Ezquerra

“…Current in vitro systems of induced pluripotent stem cells (iPSCs) generated by cell reprogramming of somatic cells from patients have provided valuable disease models recapitulating key pathological molecular events. …”
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