A patient-based iPSC-derived hepatocyte model of alcohol-associated cirrhosis reveals bioenergetic insights into disease pathogenesis by Bani Mukhopadhyay, Cheryl Marietta, Pei-Hong Shen, Abdul Oiseni, Faridoddin Mirshahi, Maria Mazzu, Colin Hodgkinson, Eli Winkler, Qiaoping Yuan, Daniel Miranda, George Kunos, Arun J. Sanyal, David Goldman

“…While differences in metabolism, inflammation, signaling, microbiome signatures and genetic variations have been tied to the pathogenesis of AC, the key underlying mechanisms for this interindividual variability, remain to be fully elucidated. Induced pluripotent stem cell-derived hepatocytes (iHLCs) from patients with AC and healthy controls differ transcriptomically, bioenergetically and histologically. …”
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Optimized gene transduction in human lung organoids: A high-efficiency method for advanced research applications by Jasmin Khateeb, Jady Liang, Yuchong Li, Thenuka Thanabalasingam, Julie Khang, Mirjana Jerkic, Giovanna Pellecchia, Bhooma Thiruv, Ya-Wen Chen, Ori Rotstein, Arthur S. Slutsky, Haibo Zhang

“…Abstract Human induced pluripotent stem cell (iPSC)-derived lung organoids, engineered to carry targeted genes, offer a robust platform for investigating mechanistic insights in lung research. …”
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N-Cadherin promotes cardiac regeneration by potentiating pro-mitotic β-Catenin signaling in cardiomyocytes by Yi-Wei Tsai, Yi-Shuan Tseng, Yu-Shuo Wu, Wei-Lun Song, Min-Yi You, Yun-Chia Hsu, Wen-Pin Chen, Wei-Han Huang, Jia-Ci Chng, Chai-Ling Lim, Ke-Hsuan Wei, Shih-Lei Ben Lai, Wen-Chih Lee, Kai-Chien Yang

“…N-Cadherin knockdown reduces, whereas overexpression increases, the proliferation activity of neonatal mouse CMs and human induced pluripotent stem cell-derived CMs. Mechanistically, N-Cadherin binds and stabilizes pro-mitotic transcription regulator β-Catenin, driving CM self-renewal. …”
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Epicardially Placed Bioengineered Cardiomyocyte Xenograft in Immune-Competent Rat Model of Heart Failure by Ikeotunye Royal Chinyere, Pierce Bradley, Joshua Uhlorn, Joshua Eason, Saffie Mohran, Giuliana G. Repetti, Sherry Daugherty, Jen Watson Koevary, Steven Goldman, Jordan J. Lancaster

“…Background. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are under preclinical investigation as a cell-based therapy for heart failure post-myocardial infarction. …”
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Human Fetal Liver: An In Vitro Model of Erythropoiesis by Guillaume Pourcher, Christelle Mazurier, Yé Yong King, Marie-Catherine Giarratana, Ladan Kobari, Daniela Boehm, Luc Douay, Hélène Lapillonne

“…Human embryonic stem (ES) cells or induced pluripotent stem cell (iPS) are the natural candidates. …”
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Extracellular Recordings of Patterned Human Pluripotent Stem Cell-Derived Cardiomyocytes on Aligned Fibers by Junjun Li, Itsunari Minami, Leqian Yu, Kiyotaka Tsuji, Minako Nakajima, Jing Qiao, Masato Suzuki, Ken Shimono, Norio Nakatsuji, Hitetoshi Kotera, Li Liu, Yong Chen

“…Human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) hold high potential for use in drug assessment and myocardial regeneration. …”
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Metabolic adaptations to acute glucose uptake inhibition converge upon mitochondrial respiration for leukemia cell survival by Monika Komza, Jesminara Khatun, Jesse D. Gelles, Andrew P. Trotta, Ioana Abraham-Enachescu, Juan Henao, Ahmed Elsaadi, Andriana G. Kotini, Cara Clementelli, JoAnn Arandela, Sebastian El Ghaity-Beckley, Agneesh Barua, Yiyang Chen, Mirela Berisa, Bridget K. Marcellino, Eirini P. Papapetrou, Masha V. Poyurovsky, Jerry Edward Chipuk

“…When wild-type and oncogenic RAS patient-derived induced pluripotent stem cell acute myeloid leukemia (AML) models were examined, KL-11743 mediated induction of mitochondrial respiration and dependency for survival associated with oncogenic RAS. …”
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Susceptibility to Ventricular Arrhythmias Resulting from Mutations in FKBP1B, PXDNL, and SCN9A Evaluated in hiPSC Cardiomyocytes by Hector Barajas-Martinez, Maya Smith, Dan Hu, Robert J. Goodrow, Colleen Puleo, Can Hasdemir, Charles Antzelevitch, Ryan Pfeiffer, Jacqueline A. Treat, Jonathan M. Cordeiro

“…We assessed the functional impact of these mutations in induced pluripotent stem cell cardiomyocytes (hiPSC-CMs) derived from the proband and an unaffected family member. …”
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NPT100-18A rescues mitochondrial oxidative stress and neuronal degeneration in human iPSC-based Parkinson’s model by Julian E. Alecu, Veronika Sigutova, Razvan-Marius Brazdis, Sandra Lörentz, Marios Evangelos Bogiongko, Anara Nursaitova, Martin Regensburger, Laurent Roybon, Kerstin M. Galler, Wolfgang Wrasidlo, Beate Winner, Iryna Prots

“…NPT100-18A and structurally similar compounds (such as NPT200-11/UCB0599, currently being assessed in clinical studies) point towards a promising new approach for disease-modification. Methods Induced pluripotent stem cell (iPSC)-derived mDANs from PD patients with a monoallelic SNCA locus duplication and unaffected controls were treated with NPT100-18A. αSyn aggregation was evaluated biochemically and reactive oxygen species (ROS) levels were assessed in living mDANs using fluorescent dyes. …”
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Cellular senescence induced by cholesterol accumulation is mediated by lysosomal ABCA1 in APOE4 and AD by Shaowei Wang, Boyang Li, Jie Li, Zhiheng Cai, Cristelle Hugo, Yi Sun, Lu Qian, Julia TCW, Helena C. Chui, Dante Dikeman, Isaac Asante, Stan G. Louie, David A. Bennett, Zoe Arvanitakis, Alan T. Remaley, Bilal E. Kerman, Hussein N. Yassine

“…Additionally, we used APOE4-TR mice and induced pluripotent stem cell (iPSC) models to explore cholesterol-ABCA1-senescence pathways. …”
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Amyloidogenic immunoglobulin light chains disturb contractile function and calcium transients in a human cardiac spheroid model of light chain (AL) amyloidosis by Mélanie Bézard, Jean-Sébastien Vartanian-Grimaldi, Julien Henri, Denisa Calin, Amira Zaroui, Mounira Kharoubi, Thibaud Damy, Onnik Agbulut, Ekaterini Kordeli

“…To mimic the disease in a reconstituted system, soluble amyloidogenic LCs purified from urine of AL-CA patients were added to a mixture of induced pluripotent stem cell-issued human cardiomyocytes (hiPSC-CM) and human primary cardiac fibroblasts, which resulted in formation of spheroids within 7 days. …”
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Integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high-fidelity drug screening by Yu-hong Wang, Ting-ting Liu, Yan-ping Guo, Shuo-ji Zhu, Zi-ming Liao, Jia-mei Song, Xi-ming Zhu, Jia-liang Liang, Moussa Ide Nasser, Nan-bo Liu, De-hua Chang, Ping Zhu, Bin Yao

“…Although human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and the engineered heart organoid have been used for drug screening and mimicking disease models, they are always limited by the immaturity and lack of functionality of the cardiomyocytes. …”
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Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells by Gergo Kovacs, Viktoria Szabo, Melinda K. Pirity

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New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells by Xiangjun He, Yin-Xiong Li, Bo Feng

“…Recent technological advances by a coupling CRISPR system with various DNA repair mechanisms have further opened up new opportunities to overcome existing challenges in knocking-in foreign DNA in human pluripotent stem cells, including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). In this review, we summarized the very recent development of CRISPR-based knock-in strategies and discussed the results obtained as well as potential applications in human ESC and iPSC.…”
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Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity by Anjana Nityanandam, Mary H. Patton, Ildar T. Bayazitov, Kyle D. Newman, Kristen T. Thomas, Stanislav S. Zakharenko

“…Summary: Human induced pluripotent stem cells (hiPSCs) can be used to generate assembloids that recreate thalamocortical circuitry displaying short-term and long-term synaptic plasticity. …”
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Engineering aortic valves via transdifferentiating fibroblasts into valvular endothelial cells without using viruses or iPS cells by Peng Tang, Fuxiang Wei, Weihua Qiao, Xing Chen, Chenyang Ji, Wanzhi Yang, Xinyu Zhang, Sihan Chen, Yanyan Wu, Mingxing Jiang, Chenyu Ma, Weiqiang Shen, Qi Dong, Hong Cao, Minghui Xie, Ziwen Cai, Li Xu, Jiawei Shi, Nianguo Dong, Junwei Chen, Ning Wang

“…The technology of induced pluripotent stem cells (iPSCs) has enabled the conversion of somatic cells into primitive undifferentiated cells via reprogramming. …”
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iPSCs: A Minireview from Bench to Bed, including Organoids and the CRISPR System by Andrés Javier Orqueda, Carla Alejandra Giménez, Federico Pereyra-Bonnet

“…Ten years later, the reprogramming process became a defined method of producing induced pluripotent stem cells (iPSCs) through the overexpression of four transcription factors. iPSCs are capable of originating virtually all types of cells and tissues, including a whole new animal. …”
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Structural characterization of stem cell factors Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to detect phospho-dependent binding partners by Bouguechtouli, Chafiaa, Ghouil, Rania, Alik, Ania, Dingli, Florent, Loew, Damarys, Theillet, Francois-Xavier

“…The combined expression of a handful of pluripotency transcription factors (PluriTFs) in somatic cells can generate induced pluripotent stem cells (iPSCs). Here, we report the structural characterization of disordered regions contained in four important PluriTFs, namely Oct4, Sox2, Nanog and Esrrb. …”
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Identification of Molecular Signatures in Neural Differentiation and Neurological Diseases Using Digital Color-Coded Molecular Barcoding by Debora Salerno, Alessandro Rosa

“…Human pluripotent stem cells (PSCs), including embryonic stem cells and induced pluripotent stem cells, represent powerful tools for disease modeling and for therapeutic applications. …”
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Protocol for differentiating cardiomyocytes and generating engineered heart tissues from human feeder-free extended pluripotent stem cells by Zhongjun Wan, Shanshan Wen, Ran Zheng, Li Li, Wei Jiang, Donghui Zhang

“…We describe steps for converting human embryonic stem cells or induced pluripotent stem cells (ESCs/iPSCs) into ffEPSCs, followed by their long-term maintenance, cryopreservation, seed preservation, and differentiation into cardiomyocytes. …”
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