Metformin and Probiotics Interplay in Amelioration of Ethanol-Induced Oxidative Stress and Inflammatory Response in an In Vitro and In Vivo Model of Hepatic Injury by Farhin Patel, Kirti Parwani, Dhara Patel, Palash Mandal

“…Endoplasmic reticulum (ER) stress markers, inflammatory markers, lipid metabolism, reactive oxygen species (ROS) production, and oxidative stress were evaluated, using qRT-PCR, Oil red O staining, fluorimetry, and HPLC. In vitro, probiotic V and Met in combination prevented ethanol-induced cellular injury, ER stress, oxidative stress, and regulated lipid metabolism as well as inflammatory response in HepG2 cells. …”
Get full text

Interactions of the anti-FcRn monoclonal antibody, rozanolixizumab, with Fcγ receptors and functional impact on immune cells in vitro by Omar S. Qureshi, Emma J. Sutton, Rosemary F. Bithell, Shauna M. West, Rona M. Cutler, Gillian McCluskey, Graham Craggs, Asher Maroof, Nicholas M. Barnes, David P. Humphreys, Stephen Rapecki, Bryan J. Smith, Anthony Shock

“…Furthermore, data from in vitro experiments using relevant human cell types that express both FcRn and FcγRI indicated no evidence for functional sequelae in relation to cellular activation events (e.g., intracellular signaling, cytokine production) upon either FcRn or FcγR binding of rozanolixizumab. …”
Get full text

Synthetic CB1 Cannabinoids Promote Tunneling Nanotube Communication, Cellular Migration, and Epithelial–Mesenchymal Transition in Pancreatic PANC-1 and Colorectal SW-620 Cancer Cel... by David A. Bunsick, Leili Baghaie, Yunfan Li, Abdulrahman M. Yaish, Emilyn B. Aucoin, Elizabeth Skapinker, Rashelle Aldbai, Myron R. Szewczuk

“…Here, we investigated the in vitro effects of the synthetic CB1 cannabinoids AM-404, arvanil, and olvanil on human pancreatic PANC-1 and colorectal SW-620 cancer cell lines to understand further cellular behaviors and the potential risks of their use in cancer therapy. …”
Get full text

Biotechnological Phytocomplex of <i>Zanthoxylum piperitum</i> (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo by Giovanna Rigillo, Giovanna Pressi, Oriana Bertaiola, Chiara Guarnerio, Matilde Merlin, Roberto Zambonin, Stefano Pandolfo, Angela Golosio, Francesca Masin, Fabio Tascedda, Marco Biagi, Giulia Baini

“…Human fibroblasts (HFFs) were treated with varying ZPP concentrations to assess cellular viability, collagen metabolism, and ECM-related enzyme activities, both under normal and cell stress conditions. …”
Get full text

Synergistic Effects of SDS and H2O2 Combinations on Tracheal Scaffold Development: An In Vitro Study Using Goat Trachea by Dhihintia Jiwangga, Ferdiansyah Mahyudin, Gondo Mastutik, Estya Nadya Meitavany, null Juliana, Priangga Adi Wiratama

“…In the development of tissue-engineered tracheal scaffolds, in vitro studies play a crucial role in allowing researchers to evaluate the efficacy and safety of scaffold designs and fabrication techniques before progressing to in vivo or clinical trials. …”
Get full text

Corrigendum to “In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some Traditionally Fermented Ethiopian Food Products” by Guesh Mulaw, Tesfaye Sisay Tessema, Diriba Muleta, Anteneh Tesfaye

Get full text

Understanding retinal tau pathology through functional 2D and 3D iPSC-derived in vitro retinal models by Lorenza Mautone, Federica Cordella, Alessandro Soloperto, Silvia Ghirga, Giorgia Di Gennaro, Ylenia Gigante, Silvia Di Angelantonio

“…Abstract The generation of retinal models from human induced pluripotent stem cells holds significant potential for advancing our understanding of retinal development, neurodegeneration, and the in vitro modeling of neurodegenerative disorders. The retina, as an accessible part of the central nervous system, offers a unique window into these processes, making it invaluable for both study and early diagnosis. …”
Get full text

Food nutrition and toxicology targeting on specific organs in the era of single-cell sequencing by Xiaofei Wang, Xiaowen Cheng, Huiling Liu, Xiaohuan Mu, Hao Zheng

“…However, the limitations of traditional in vitro assays or in vivo animal experiments cannot comprehensively examine cellular heterogeneity and the tissue-biased influences. …”
Get full text

THE MAIN REASONS OF THE LOW EFFICIENCY OF OBTAINING ALUMINUM-RESISTANT REGENERANTS OF CEREAL CROPS by E. M. Lisitsyn

Get full text

Reactivity in cell culture medium and in vitro anticancer activity of 3,5-di-tert-butylcatechol: link to metal-catechol interactions by Aviva Levina, Debbie C. Crans, Peter A. Lay

Get full text

Construction of complex three-dimensional vascularized liver tissue model in vitro based on a biphasic cell-laden embedding medium by Weikang Lv, Haoran Yu, Abdellah Aazmi, Tuya Naren, Wanli Cheng, Mengfei Yu, Zhen Wang, Xiaobin Xu, Huayong Yang, Liang Ma

“…Constructing an in vitro vascularized liver tissue model that closely simulates the human liver is crucial for promoting cell proliferation, mimicking physiological heterogeneous structures, and recreating the cellular microenvironment. …”
Get full text

DNA hypomethylation-mediated upregulation of GADD45B facilitates airway inflammation and epithelial cell senescence in COPD by Yuan Zhan, Qian Huang, Zhesong Deng, Shanshan Chen, Ruonan Yang, Jiaheng Zhang, Yating Zhang, Maocuo Peng, Jixing Wu, Yiya Gu, Zhilin Zeng, Jungang Xie

“…Eventually, in vivo and vitro experiments demonstrated that GADD45B overexpression was partially mediated by CS-induced DNA hypomethylation. …”
Get full text

Insights into the relations between cell wall integrity and in vitro digestion properties of granular starches in pulse cotyledon cells after dry heat treatment by Ping Li, Bin Zhang, Rui Liu, Li Ding, Xiong Fu, Haiteng Li, Qiang Huang, Xiaowei He

Get full text

Overexpression of metalloproteinase PAPPA accelerates cancer progression and correlates with immune cell infiltration in gastric cancer: insights from bioinformatics and in vitro i... by Shiya Yu, Shiyao Zheng, Jinwei Qiu, Hongming Lin, Xiaojuan Yu, Huiyan Chen, Junhong Wu, Weihang Wu, Jiong Chen, Yongyuan Chen, Jianwei Chen, Hu Zhao, Yu Wang

Get full text

Comprehensive Physicochemical Characterization and in Vitro Human Cell Culture Studies of an Innovative Biocompatible and Biodegradable Silk-Derived Protein Hydrolysate, SDP‑4 by Hasham Shafi, Andrea J. Lora, Saurabh Aggarwal, David W. Infanger, Brian D. Lawrence, Heidi M. Mansour

Get full text

Enhancing DNA Vaccine Delivery Through Stearyl-Modified Cell-Penetrating Peptides: Improved Antigen Expression and Immune Response In Vitro and In Vivo by Sheng Jiang, Cheng Zu, Bin Wang, Yiwei Zhong

“…Background: Inefficient cellular uptake is a significant limitation to the efficacy of DNA vaccines. …”
Get full text

Gingival fibroblast suppress the osteogenesis process mediated by bone substitute materials via WNT/β-catenin signaling pathway in vitro and in vivo by Guanqi Liu, Jiahui Lin, Xiaoyan Chen, Runheng Liu

“…These findings highlight the importance of considering the cellular microenvironment in tissue engineering and regenerative medicine and suggest potential targets for modulating MMSCs behavior to enhance bone regeneration.…”
Get full text

In vitro and molecular docking evaluation of black sesame seeds' anti-prostate cancer and antioxidant activity processed by nine steaming nine drying by Glennielyn D. Magdamit, Cheng-Yang Hsieh, Mon-Juan Lee, Kathlia A. De Castro-Cruz, Samantha Franchette B. Austria, Beatrice D. Sipat, Steven Kuan-Hua Huang, Po-Wei Tsai

Get full text

In Vitro Expansion of Bone Marrow Derived Mesenchymal Stem Cells Alters DNA Double Strand Break Repair of Etoposide Induced DNA Damage by Ian Hare, Marieta Gencheva, Rebecca Evans, James Fortney, Debbie Piktel, Jeffrey A. Vos, David Howell, Laura F. Gibson

“…To investigate the response to DSB stress with passage in vitro, primary human MSCs were exposed to etoposide (VP16) at various passages with subsequent evaluation of cellular damage responses and DNA repair. …”
Get full text

An In Vitro Pilot Study Comparing the Novel HemoClear Gravity-Driven Microfiltration Cell Salvage System with the Conventional Centrifugal XTRA™ Autotransfusion Device by Anneloes Hoetink, Sabine F. Scherphof, Frederik J. Mooi, Paul Westers, Jack van Dijk, Sjef J. van de Leur, Arno P. Nierich

Get full text