Evaluation of maSSS/maSES-PEG2-RM26 for their potential therapeutic use after labeling with Re-188. Could their [99mTc]Tc-labeled counterparts be used to estimate dosimetry? by Panagiotis Kanellopoulos, Quanyi Yu, Abouzayed Abouzayed, Ekaterina Bezverkhniaia, Vladimir Tolmachev, Anna Orlova

“…Results Both peptides were successfully labeled with Re-188 and evaluated both in vitro and in vivo. In GRPR expressing PC-3 cells, both [188Re]Re-labeled peptides displayed high cellular uptake (8.5 ± 0.1% and 5 ± 0.3% of added activity, respectively), heavily GRPR-driven, while retaining the radioantagonistic profile with slow internalization rates. …”
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Nanocomposite magnetic hydrogel with dual anisotropic properties induces osteogenesis through the NOTCH-dependent pathways by Shijia Tang, Yue Yan, Xiaoli Lu, Peng Wang, Xueqin Xu, Ke Hu, Sen Yan, Zhaobin Guo, Xiao Han, Feimin Zhang, Ning Gu

“…Abstract Physical factors in the cellular microenvironment have critical effects on stem cell differentiation. …”
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SIRT5‐mediated HOXA5 desuccinylation inhibits ferroptosis to alleviate sepsis induced‐lung injury by Lei Wang, Heng Fan, Min Sun, Ji‐Hui Ye

“…Cell viability, ferrous iron (Fe2+) level, and cellular lipid reactive oxygen species (ROS) were determined with Cell Counting Kit‐8 assay, iron assay kit, and BODIPY™ 665/676 molecular probe, respectively. …”
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Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells by Fraser I. Young, Vsevolod Telezhkin, Sarah J. Youde, Martin S. Langley, Maria Stack, Paul J. Kemp, Rachel J. Waddington, Alastair J. Sloan, Bing Song

“…Cellular heterogeneity presents an important challenge to the development of cell-based therapies where there is a fundamental requirement for predictable and reproducible outcomes. …”
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Identification of natural phytochemicals as AKT2 inhibitors using molecular docking and dynamics simulations as potential cancer therapeutics by Jibon Kumar Paul, Mahir Azmal, Md Naimul Haque Shohan, Mohua Mrinmoy, ANM Shah Newaz Been Haque, Omar Faruk Talukder, Ajit Ghosh

“…The PI3K/AKT/mTOR pathway is central in regulating key cellular processes such as proliferation, survival, metabolism, and angiogenesis. …”
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Interaction of the Wolbachia surface protein with a novel pro-viral protein from Aedes aegypti by Mazhar Hussain, Zhi Qi, Sassan Asgari

“…To date, several reports have appeared that highlight multiple molecular and cellular pathways involved in the blocking mechanism, which underlines the complicated nature of the mechanism. …”
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Scalable Manufacturing Method for Model Protein-Loaded PLGA Nanoparticles: Biocompatibility, Trafficking and Release Properties by Selin Akpinar Adscheid, Marta Rojas-Rodríguez, Salma M. Abdel-Hafez, Francesco S. Pavone, Marc Schneider, Akif E. Türeli, Martino Calamai, Nazende Günday-Türeli

“…<b>Results</b>: The formulation demonstrated sustained and prolonged protein release profiles. Importantly, cellular uptake studies revealed that the NPs were not internalized. …”
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MiR-181a-5p Delivered by Adipose-Derived Mesenchymal Stem Cell Exosomes Alleviates Klebsiella pneumonia Infection-Induced Lung Injury by Targeting STAT3 Signaling by Ren-Jing Hu, Xiao-Chun Chen, Lei Xu, Xiao-Hong Rui, Lin Wan, Jie Lu, Jun Liu, Hao Pei

“…ADSCs and exosomes were extracted and characterized in vitro. The translocation of ADSC-derived exosomes to bone marrow-derived macrophages (BMDMs) was detected. …”
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Enucleated bone marrow-derived mesenchymal stromal cells regulate immune microenvironment and promote testosterone production through efferocytosis by Lu Sun, Jiayu Huang, Xuezi Wang, Peng Huang, Baolin Dong, Zehang Liang, Jiahong Wu, Jiancheng Wang

“…Stem cell therapy has been wildly studied in vitro. However, due to the multidirectional differentiation potential and heterogeneity of stem cells, it is difficult to achieve the good efficiency and reproducibility in basic research and clinical applications. …”
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Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα by Sujuan Xu, Edward Lee, Zhaoxing Sun, Xiaoyan Wang, Ting Ren, Zhouping Zou, Jifu Jin, Jie Li, Jian Zhang, Yingxiang Li, Qiang Yang, Yang Zhang, Man Guo, Yi Fang, Xiaoqiang Ding

“…Renal proximal tubular cells are susceptible to oxidative stress, and the dysregulation of renal proximal tubular cellular homeostasis can damage cells via apoptotic pathways. …”
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Identification and validation of CDK1 as a promising therapeutic target for Eriocitrin in colorectal cancer: a combined bioinformatics and experimental approach by Jiemiao Shen, Xing Gong, Haili Ren, Xia Tang, Hairong Yu, Yilu Tang, Shen Chen, Minghui Ji

“…Knockdown of CDK1 inhibited the proliferation, migration, and invasion of CRC cells in vitro. Furthermore, Eriocitrin emerged as a potential inhibitor, exerting its anti-tumor effects by targeting and inhibiting CDK1 activity. …”
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PRMT5 Inhibitor EPZ015666 Decreases the Viability and Encystment of <i>Entamoeba invadens</i> by Rigoberto Ortiz-Hernández, Elmer Joel Millán-Casarrubias, Jeni Bolaños, Susana Munguía-Robledo, Carlos Vázquez-Calzada, Elisa Azuara-Licéaga, Jesús Valdés, Mario Alberto Rodríguez

“…Protein arginine methyltransferase 5 (PRMT5) is an enzyme that produces monomethyl arginine (MMA) and symmetric dimethyl arginine (sDMA), post-translational modifications that regulate several cellular processes, including stage conversion in parasitic protozoans. …”
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Effectively alleviate rheumatoid arthritis via maintaining redox balance, inducing macrophage repolarization and restoring homeostasis of fibroblast-like synoviocytes by metformin-... by Rui Zhang, Xingyu Lin, Rongjie Lin, Zhenbin Chen, Chenfang Miao, Yao Wang, Xiaoqin Deng, Jianlong Lin, Shishui Lin, Shaohuang Weng, Min Chen

“…In this study, we successfully synthesized metformin-derived carbon dots (MCDs), and investigated the antirheumatic effect in vivo and in vitro. Designed MCDs could target inflamed cells and accumulate at the inflammatory joints of collagen-induced arthritis (CIA) rats. …”
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Multi-Omics and Network-Based Drug Repurposing for Septic Cardiomyopathy by Pei-Pei Liu, Xin-Yue Yu, Qing-Qing Pan, Jia-Jun Ren, Yu-Xuan Han, Kai Zhang, Yan Wang, Yin Huang, Tao Ban

“…Among them, acetaminophen and pyridoxal phosphate significantly improved cardiac function in SCM moues, as demonstrated by the increased ejection fraction (EF) and fractional shortening (FS), and the reduced levels of cardiac injury biomarkers: B-type natriuretic peptide (BNP) and cardiac troponin I (cTn-I). In vitro assays revealed that acetaminophen inhibited prostaglandin synthesis, reducing inflammation, while pyridoxal phosphate restored amino acid balance, supporting cellular function. …”
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Antioxidant and anti-inflammatory function of walnut green husk aqueous extract (WNGH-AE) on human hepatocellular carcinoma cells (HepG2) treated with t-BHP. by Zheng-Qiang Li, Yu-Ting Gao, Chen-Fa Zhao, Rui An, Yan-Lv Wu, Zhi-Pang Huang, Ping Ma, Xu Yang, Rong She, Xiao-Yan Yang

“…In this study, we aimed to investigate the effectiveness of walnut green husk (WNGH) as an antioxidant and anti-inflammatory agent in an in vitro setting. HepG2 cells were treated with tert-butyl hydroperoxide (t-BHP) to establish a cellular model of oxidative damage and inflammation. …”
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A Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Analysis of Primary Cultured Cells and Subcultured Cells Using Mouse Adipose-Derived Mesenchymal Stem Cells by Yoshiki Nakashima, Saifun Nahar, Chika Miyagi-Shiohira, Takao Kinjo, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Jiro Fujita, Hirofumi Noguchi

“…A total of 256 proteins were classified as cellular process-related proteins, while 179 were classified as metabolic process-related proteins in P0. …”
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Efficient Dual Cas9 Nickase Correction of a Prevalent Pathogenic LAMB3 Variant for Junctional Epidermolysis Bullosa by Alex du Rand, John Hunt, Daniel Verdon, Ben Buttle, P. Rod Dunbar, Diana Purvis, Vaughan Feisst, Hilary Sheppard

“…Dual-Cas9n–corrected JEB keratinocytes demonstrated restored laminin-332 expression and secretion in vitro, leading to improved cellular adhesion and accurate laminin-332 localization in engineered skin equivalents. …”
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Glutamine-glutamate centered metabolism as the potential therapeutic target against Japanese encephalitis virus-induced encephalitis by Mengyuan Li, Hang Yuan, Xiaofei Yang, Yingfeng Lei, Jianqi Lian

“…Given that viruses are obligate intracellular parasites, cellular metabolic reprogramming triggered by viral infection is intricately related to the establishment of infection and progression of disease. …”
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Pre-Conditioning with CDP-Choline Attenuates Oxidative Stress-Induced Cardiac Myocyte Death in a Hypoxia/Reperfusion Model by Héctor González-Pacheco, Aurelio Méndez-Domínguez, Salomón Hernández, Rebeca López-Marure, Maria J. Vazquez-Mellado, Cecilia Aguilar, Leticia Rocha-Zavaleta

“…CDP-choline is a key intermediate in the biosynthesis of phosphatidylcholine, which is an essential component of cellular membranes, and a cell signalling mediator. …”
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Phytochemical and toxicological evaluation of aqueous leaf extract of Premna integrifolia L. in male balb/c mice by Suman, Pratibha Gaurav, Rajesh Saini, Kavindra Nath Tiwari, Gautam Geeta Jiwatram

“…Identification of pharmacologically active compounds in ALEPI was performed using UHPLC-HRMS analysis. In vitro, estimation of Polyphenol Content, Total Flavonoid Content, saponins and Antioxidant Properties of ALEPI was performed. …”
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